Article
Plant Sciences
Maxime Versluys, Ebru Toksoy Oner, Wim Van den Ende
Summary: This study found that chicory can detect both extracellular levan oligosaccharides (LOS) of microbial origin and extracellular small inulin-type fructooligosaccharides (FOS) of endogenous origin. It also discovered that sulfated LOS has a direct antifungal effect and can trigger an increase in apoplastic sugar concentrations. These findings provide new insights into the immune response of plants.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Microbiology
Judith Pozo de la Hoz, Javier Rivero, Concepcion Azcon-Aguilar, Miguel Urrestarazu, Maria J. Pozo
Summary: The intensity of light plays a crucial role in the establishment of mycorrhizal symbioses, influencing plant growth, nutrition, and resistance to pathogens. Under different light intensities, mycorrhizal symbioses efficiently reduced disease incidence and severity, providing biotic stress protection regardless of nutritional or growth benefits.
Article
Plant Sciences
Daniel De Vega, Nicola Holden, Pete E. Hedley, Jenny Morris, Estrella Luna, Adrian Newton
Summary: Current crop protection strategies against Botrytis cinerea rely on conventional fungicides and host genetic resistance, but these strategies are not sufficient due to pathogen evolution and fungicide regulations. Defence elicitors like chitosan can prime plant defense mechanisms for faster and stronger resistance expression upon pathogen recognition. This study identified novel tomato genes, ACRE75 and ACRE180, which act as positive regulators of plant resistance against B. cinerea, providing valuable information for protecting Solanaceae plants.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Plant Sciences
Moira Giovannoni, Lucia Marti, Simone Ferrari, Natsuki Tanaka-Takada, Masayoshi Maeshima, Thomas Ott, Giulia De Lorenzo, Benedetta Mattei
Summary: This study demonstrates the essential role of the protein PCaP1 in mediating late responses to oligogalacturonides and flagellin. PCaP1 is not involved in early responses but may be important for maintaining its stability on the plasma membrane through endocytic turnover. The findings suggest a mechanism of PCaP1 endocytosis induced by oligogalacturonides.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Plant Sciences
Moira Giovannoni, Lucia Marti, Simone Ferrari, Natsuki Tanaka-Takada, Masayoshi Maeshima, Thomas Ott, Giulia De Lorenzo, Benedetta Mattei
Summary: The study reveals the crucial role of PCaP1 in plant immune responses triggered by OGs and flagellin, mainly impacting late responses and affecting the responsiveness of plants to a second treatment with OGs. Localization studies on PCaP1 post-OG treatment indicate its presence on the plasma membrane with specific structures, as well as its association with endocytic vesicles.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Maxime Versluys, Wim Van den Ende
Summary: Research investigated the use of fructan-induced immunity against Botrytis cinerea in crops. Results showed that immune resistance significantly increased after priming with natural and sulfated levan oligosaccharides (LOS). Fructo-oligosaccharides (FOS), inulin, and branched-type fructans had no clear positive effects. Only sulfated LOS induced a direct ROS burst, while LOS acted as a priming compound. Total leaf sugar levels increased after LOS priming and subsequent infection, with a temporary increase in apoplastic sugar levels after LOS priming. Dynamics of LOS and small soluble sugars in the apoplast showed a temporal peak.
Article
Plant Sciences
Yanwei Wang, Jing Sun, Chen Deng, Shouzhen Teng, Guoxin Chen, Zhenhua Chen, Xuean Cui, Thomas P. Brutnell, Xiao Han, Zhiguo Zhang, Tiegang Lu
Summary: This study identified the role of the SEM1 gene in regulating sucrose transport in rice by analyzing two mesophyll starch excess mutants. Through characterization and experimental validation, the relationship between sucrose and vascular cell development was revealed.
Article
Plant Sciences
Ping Gao, Rong Zeng, Shigang Gao, Lihui Xu, Zhiwei Song, Fuming Dai
Summary: Fluxapyroxad has been used for over 5 years to control gray mold on strawberries in China. However, resistance to this fungicide has become a threat to its effectiveness. Recent testing revealed an increased frequency of resistance in Botrytis cinerea isolates collected in 2021 compared to 2020.
Article
Plant Sciences
Ngoc Huu Nguyen, Patricia Trotel-Aziz, Sandra Villaume, Fanja Rabenoelina, Christophe Clement, Fabienne Baillieul, Aziz Aziz
Summary: Camalexin plays a significant role in induced systemic resistance (ISR) against Botrytis cinerea and Pseudomonas syringae Pst DC3000 in Arabidopsis. The analysis of CYP71A12 and PAD3 mutants revealed that loss of camalexin synthesis affects ISR against B. cinerea, but not always against Pst DC3000. Additionally, experiments with Arabidopsis mutants compromised in different hormonal signaling pathways highlighted the importance of salicylic acid in ISR camalexin accumulation upon infection with both pathogens.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Multidisciplinary Sciences
Richa Thakur, Rajni Devi, Milan Kumar Lal, Rahul Kumar Tiwari, Sucheta Sharma, Ravinder Kumar
Summary: Biotic stress caused by fungal infection has a negative impact on the growth and development of chickpea. This study compared the response of a resistant genotype (C. pinnatifidum) and a susceptible genotype (PBG5) to Botrytis cinerea infection, and found morphological, ultrastructural, and molecular differences between them. Fluorescence microscopy revealed a higher stomatal index in the resistant genotype. PCR analysis also identified a Botrytis responsive gene in the resistant genotype, potentially contributing to its resistance against grey mould.
Article
Plant Sciences
Le Chen, Baixin Sun, Yang Zhao, Peng Xiang, Zeyan Miao
Summary: Botrytis cinerea is a common phytopathogen that causes significant losses in plant cultivation worldwide. The study found that the phenylpyrrole fungicide fludioxonil has high activity against B. cinerea, but resistant isolates have been found in tomato greenhouses. The resistant isolates showed reduced fitness, including sporulation, pathogenicity, mycelial growth, and hypersensitivity to osmotic stress.
Article
Agronomy
Lincoln A. Harper, Scott Paton, Barbara Hall, Suzanne McKay, Richard P. Oliver, Francisco J. Lopez-Ruiz
Summary: Botrytis bunch rot, caused by Botrytis cinerea, is a significant disease in Australian grape-growing regions. Control relies on canopy management and fungicide application, but the latter can lead to resistance selection. The study found relatively low resistance frequencies and recommended continued monitoring of critical fungicide modes of action in Australian vineyards.
PEST MANAGEMENT SCIENCE
(2022)
Article
Horticulture
Yuanhua Wang, Fengxia Zhao, Geng Zhang, Sizhen Jia, Zhiming Yan
Summary: Plant WRKY transcriptional factors are crucial in defense responses against biotic stresses, with FaWRKY11 found to positively regulate defense in strawberry fruit against Botrytis cinerea. Overexpression of FaWRKY11 significantly alleviated tissue damage in strawberries, while its silencing led to slightly increased disease incidence. Variations in gene expression levels related to disease resistance and phytohormone metabolism were observed, suggesting a role of FaWRKY11 in regulating defense mechanisms against B. cinerea in strawberries.
SCIENTIA HORTICULTURAE
(2021)
Article
Biotechnology & Applied Microbiology
Hongyi Liu, Shaoqing Wang, Bo Lang, Yaqian Li, Xinhua Wang, Jie Chen
Summary: In this study, it was found that the engineered Trichoderma afroharzianum strain OE:SCf, which expresses the fused Sm1-Chit42 protein, exhibited synergistic inhibition against Botrytis cinerea growth and reproduction. The SCf protein showed inhibitory effects at multiple stages of the mycoparasitic interaction between T. afroharzianum and B. cinerea, including chemotropism sensing, hyphal coiling, hydrophobicity modulation, cell wall adhesion, virulence reduction, and pathogen killing by ROS. These findings highlight a novel mycoparasitic system in Trichoderma strains engineered with chimeric proteins to combat B. cinerea, providing a solid foundation for future development of engineered Trichoderma biofungicides created with chimeric proteins.
MICROBIAL CELL FACTORIES
(2023)
Article
Agronomy
Seiya Saito, Fei Wang, Chang-Lin Xiao
Summary: This study evaluated the efficacy of natamycin as an alternative fungicide for controlling gray mold of blueberries. The results showed that natamycin significantly reduced gray mold incidence and lesion size, and decreased overall fruit decay caused by natural infections, without adverse effects on fruit quality.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Microbiology
Isaac A. Salmeron-Santiago, Miguel Martinez-Trujillo, Juan J. Valdez-Alarcon, Martha E. Pedraza-Santos, Gustavo Santoyo, Maria J. Pozo, Ana T. Chavez-Barcenas
Summary: Arbuscular mycorrhizal fungi (AMF) modify carbon distribution in plants by altering key enzymes involved in sucrose and lipid metabolism, thus influencing plant growth and development.
Article
Plant Sciences
Lisanne Smulders, Victoria Ferrero, Eduardo de la Pena, Maria J. Pozo, Juan Antonio Diaz Pendon, Emilio Benitez, Alvaro Lopez-Garcia
Summary: This study evaluates the impact of plant traits on root-associated bacterial communities and finds that plant resistance traits and genetic relatedness play important roles in shaping the composition of these communities. Furthermore, there is a positive correlation between plant resistance traits and bacterial diversity. These results highlight the potential of breeding to affect plant microbiomes.
Article
Biotechnology & Applied Microbiology
Sara Selma, Neus Sanmartin, Ana Espinosa-Ruiz, Silvia Gianoglio, Maria Pilar Lopez-Gresa, Marta Vazquez-Vilar, Victor Flors, Antonio Granell, Diego Orzaez
Summary: Transcriptional regulators based on CRISPR architecture allow for the reprogramming of gene expression in plants. In this study, the multiplexable CRISPR activator dCasEV2.1 was used to selectively enrich four different flavonoids in Nicotiana benthamiana leaves. Resulting metabolic analysis showed a specific enrichment of the targeted metabolites, demonstrating the potential of dCasEV2.1 for customizing plant metabolome.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Agronomy
Ada Frattini, Maria Martinez-Solis, Angel Llopis-Gimenez, Maria J. Pozo, Javier Rivero, Cristina M. Crava, Salvador Herrero
Summary: This study provides the first evidence of compatibility between tomato plants and AMF symbiosis with bacterial and viral entomopathogens, contributing to the development of novel approaches for biological pest control.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
J. Pastor-Fernandez, P. Sanchez-Bel, J. Gamir, V. Pastor, N. Sanmartin, M. Cerezo, S. Andres-Moreno, V. Flors
Summary: The research explores the molecular mechanisms of Systemin-induced resistance against Plectosphaerella cucumerina in Arabidopsis plants, revealing the activation of protein complexes and signaling events by Systemin, as well as the involvement of phenolic and indolic compounds in Sys-IR.
Article
Biotechnology & Applied Microbiology
Guadalupe Zitlalpopoca-Hernandez, Maria J. Pozo, Thure P. Hauser, Nicolai Meyling
Summary: Co-inoculations of different beneficial fungi can reduce the lesion sizes of the phytopathogen Botrytis cinerea and promote plant growth. However, obtaining consistent results of plant-microbe-phytopathogen interactions remains a challenge.
BIOLOGICAL CONTROL
(2022)
Article
Agronomy
Patryk Frackowiak, Barbara Wrzesinska, Przemyslaw Wieczorek, Paloma Sanchez-Bel, Laura Kunz, Antje Dittmann, Aleksandra Obrepalska-Steplowska
Summary: This study investigated the effects of benzo(1,2,3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) treatment on the defense and developmental processes in tomato plants. Multi-omics analyses revealed that BTH treatment significantly influenced the plant's immune response and activation of certain genes, as well as the accumulation of important phytohormones. The study also uncovered the involvement of BTH in the induction of thiamine metabolism in tomatoes. These findings provide valuable insights into the plant's defense mechanisms against virus infections.
Article
Plant Sciences
Beatriz Ramirez-Serrano, Marina Querejeta, Zhivko Minchev, Jordi Gamir, Elfie Perdereau, Maria J. Pozo, Geraldine Dubreuil, David Giron
Summary: Mycorrhizal symbiosis affects the growth of maize and the performance of Spodoptera exigua, but the effects are dependent on nitrogen availability. The reduced performance of the herbivore is potentially due to the nitrogen-mycorrhiza interaction and its impact on plant nutritional value, rather than changes in the gut microbiota.
JOURNAL OF PLANT INTERACTIONS
(2022)
Article
Plant Sciences
Isaac A. Salmeron-Santiago, Miguel Martinez-Trujillo, Juan J. Valdez-Alarcon, Martha E. Pedraza-Santos, Gustavo Santoyo, Pedro A. Lopez, John Larsen, Maria J. Pozo, Ana T. Chavez-Barcenas
Summary: The exchange of P and C between plants and AMF is crucial for their mutualistic symbiosis. Tomato plants inoculated with R. irregularis showed increased growth response under Low P, but similar response to non-inoculated plants under High P. AMF colonization was reduced under High P, but accumulation of AMF biomass and spores was higher under Low P. Our results suggest that P-limiting conditions can stimulate plant growth through increased source and sink strength, which is influenced by AMF performance.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Plant Sciences
Javier Lidoy, Estefania Berrio, Marta Garcia, Luis Espana-Luque, Maria J. Pozo, Juan Antonio Lopez-Raez
Summary: A study found that flavonoids serve as signaling molecules in AM symbiosis, promoting spore germination and root colonization. This discovery opens up possibilities for using flavonoids to enhance symbiosis in agricultural products.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Laura Dejana, Beatriz Ramirez-Serrano, Javier Rivero, Jordi Gamir, Juan A. Lopez-Raez, Maria J. Pozo
Summary: The effect of phosphorus fertilization on plant and pest performance, as well as plant resistance to pathogens and insect herbivores, is dependent on the mycorrhizal status of the plant. The enhanced resistance in mycorrhizal plants is influenced by phosphorus availability and the priming of plant defenses.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Microbiology
Julia Pastor-Fernandez, Paloma Sanchez-Bel, Victor Flors, Miguel Cerezo, Victoria Pastor
Summary: The plant immunity system is gaining more attention as new elements and roles are discovered. Phytocytokines are being recognized as a part of a compound family that can amplify the immune response. This review aims to highlight the role of phytocytokines in the complex plant perception and signaling events during the immune response to biotic stress.
Article
Plant Sciences
Javier Lidoy, Alvaro Lopez-Garcia, Clara Amate, Juan Manuel Garcia, Victor Flors, Jose Manuel Garcia-Garrido, Concepcion Azcon-Aguilar, Juan Antonio Lopez-Raez, Maria Jose Pozo
Summary: This study compares the colonization abilities of two arbuscular mycorrhizal fungal species, Funneliformis mosseae and Rhizophagus irregularis, in tomato plants under different stress conditions. The results show that the root colonization by the two fungi differs depending on the stress treatment and is correlated with changes in nutrient exchange, plant defensive responses, and symbiosis regulatory genes. These findings suggest that the benefits provided by each fungal species influence the carbon reward and control the root colonization by the host plant.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2023)
