Article
Polymer Science
Guangyue Zu, Marnix Meijer, Olga Mergel, Heng Zhang, Patrick van Rijn
Summary: The hierarchical nature of ECM allows for storage of small biomolecules, while nanogels are versatile tools in nanomedicine due to their simplicity of chemical control and biological compatibility. This study presents a stable hierarchical nanogel-GelMA composite hydrogel system with the potential to achieve in situ delivery and controllable release of bioactive molecules in 3D cell culture systems.
Article
Engineering, Biomedical
Simon C. Kellaway, Victoria Roberton, Joshua N. Jones, Rabea Loczenski, James B. Phillips, Lisa J. White
Summary: Incorporating dECMh derived from bone, liver, and small intestinal tissues can promote neurite extension and axonal regeneration, providing an alternative to current collagen I hydrogel.
ACTA BIOMATERIALIA
(2023)
Article
Polymer Science
Jian Guan, Fu-zhen Yuan, Zi-mu Mao, Hai-lin Zhu, Lin Lin, Harry Huimin Chen, Jia-kuo Yu
Summary: The 3D-printed PEGDA-GelMA-CSMA scaffolds not only possess adequate mechanical strength, but also maintain a suitable 3D microenvironment for differentiation, proliferation, and extracellular matrix production of bone marrow mesenchymal stem cells (BMSCs).
Article
Biotechnology & Applied Microbiology
Jun Li, Wenzhao Wang, Mingxin Li, Ping Song, Haoyuan Lei, Xingyu Gui, Changchun Zhou, Lei Liu
Summary: This study developed a photocrosslinked biomimetic methacrylated gelatin (Bio-GelMA) hydrogel scaffold to simulate the natural bone extracellular matrix, providing a 3D template and extracellular matrix microenvironment. Bone marrow mesenchymal stem cells (BMSCS) encapsulated in Bio-GelMA scaffolds showed therapeutic effects in a 3D environment for segmental bone defects, with good biocompatibility and mechanical properties. In vivo rat model experiments demonstrated that BMSC-loaded Bio-GelMA promoted robust new bone formation, indicating its potential for future clinical applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Chun Liu, Ting Dai, Xiaoyu Wu, Jiayi Ma, Jun Liu, Siyu Wu, Lei Yang, Hongbin Zhao
Summary: This study investigated the potential of nano-attapulgite (nano-ATP)/GelMA composite hydrogels for bone tissue engineering. The incorporation of nano-ATP improved the printability and mechanical properties of the hydrogels, resulting in enhanced bone regeneration and angiogenesis. This research highlights the clinical practicality of nano-ATP for bone defect repair.
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Adolf Krige, Ulrika Rova, Paul Christakopoulos
Summary: Microbial Electrosynthesis (MES) is emerging as a promising technology for decarbonization, using CO2 as feedstock for third generation biofuels. A study introduces 3D bio-printing for a synthetic biofilm, enhancing acetate production and reducing start-up time in MES reactors. The synthetic biofilm allows dense growth of S. ovata cells at the cathode, improving electron transfer efficiency and potentially catalyzation of H-2.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Engineering, Biomedical
Bin Jiang, Wenquan Ou, James G. Shamul, Hao Chen, Sarah Van Belleghem, Samantha Stewart, Zhenguo Liu, John P. Fisher, Xiaoming He
Summary: The use of Rock inhibitor (RI) in 3D cardiac differentiation of human induced pluripotent stem cells (iPSCs) poses challenges, but reducing the RI concentration can improve efficiency and homogeneity, which is important for understanding and treating heart diseases.
BIOACTIVE MATERIALS
(2022)
Article
Engineering, Biomedical
Amal George Kurian, Rajendra K. Singh, Kapil D. Patel, Jung-Hwan Lee, Hae-Won Kim
Summary: Polymeric hydrogels are fascinating platforms for tissue repair and delivery systems. Methacrylated gelatin (GelMA) is a representative hydrogel formulation that has found various biomedical applications. Recent efforts have focused on combining GelMA with bioactive and functional nanomaterials to enhance its properties and create multifunctional platforms for tissue therapeutics.
BIOACTIVE MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Alexandra Bayles, Tazio Pleij, Martin Hofmann, Fabian Hauf, Theo Tervoort, Jan Vermant
Summary: Polymer hydrogels, with their biocompatibility, stimuli responsiveness, and affordability, find broad application as advanced biomaterials and functional materials. A continuous processing scheme using laminar flow is reported to direct the organization of cross-linking density across a single sample, enabling the production of structured hydrogel filaments for soft actuator and sensor applications.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Emergency Medicine
Yulan Huang, Wenbi Wu, Haofan Liu, Yuwen Chen, Bo Li, Zhiyuan Gou, Xun Li, Maling Gou
Summary: Nerve guide conduits (NGCs) have been explored as advanced tools for peripheral nerve injury treatment, with 3D printing technologies showing great potential in fabricating functional NGCs. Various printing methods and construction strategies for functional NGCs are discussed in this review, highlighting the challenges and prospects for their development.
Article
Oncology
Hongcheng Zhang, Jinhui Xu, Saijilafu
Summary: The use of GelMA hydrogel in treating spinal cord injury in mice resulted in enhanced nerve regeneration and recovery of movement function. Injection of GelMA hydrogel reduced the area of syringomyelia and inhibited scar formation, while also promoting axon growth through the regulation of specific proteins.
ANNALS OF TRANSLATIONAL MEDICINE
(2021)
Article
Engineering, Biomedical
Wenbi Wu, Yinchu Dong, Haofan Liu, Xuebing Jiang, Ling Yang, Jing Luo, Yu Hu, Maling Gou
Summary: In this study, an elastic hydrogel conduit encapsulating with drug-releasable nanoassemblies was fabricated using a 3D printing technique, showing potential for promoting nerve regeneration. The conduit sustainedly released the drug and facilitated axon elongation, resulting in axon regeneration, remyelination, and function recovery. These findings suggest that the drug-loaded hydrogel conduit has potentials for the treatment of long-gap peripheral nerve injury.
MATERIALS TODAY BIO
(2023)
Article
Engineering, Biomedical
Kang Yu, Xinjie Zhang, Yuan Sun, Qing Gao, Jianzhong Fu, Xiujun Cai, Yong He
Summary: Projection-based 3D bioprinting (PBP) is suitable for printing delicate structures, but the requirements for printing details with photocurable bioink are not well understood. This study systematically investigates the printing details using GelMA-based bioink as an example, establishes quantized models for evaluating printing errors, and presents better strategies for bioprinting different structures.
BIOACTIVE MATERIALS
(2022)
Article
Chemistry, Applied
Eva D. Carvalho, Miguel R. G. Morais, Ana P. Pego, Cristina C. Barrias, Marco Araujo
Summary: Biofunctionalization of polysaccharides is commonly used to obtain ECM-mimicking biomaterials. In this study, the performance of carbodiimide and reductive amination chemistries for the synthesis of peptide-alginate hybrid hydrogels was compared. Reductive amination showed improved peptide grafting efficiency and allowed for adjusting the gelation, stiffness, and degradability of the hydrogels. The resulting hydrogels better supported the 3D culture of primary astrocytes.
CARBOHYDRATE POLYMERS
(2024)
Article
Polymer Science
Rebeca Leu Alexa, Horia Iovu, Jana Ghitman, Andrada Serafim, Cristina Stavarache, Maria-Minodora Marin, Raluca Ianchis
Summary: The focus of this study was on developing suitable GelMA hydrogel inks for 3D printing in tissue engineering applications. By exploring different degrees of GelMA methacrylation, concentrations of GelMA, and concentrations of photoinitiator, the researchers were able to determine the optimal formulations for the printing process. The presence of methacryloyl groups, the isoelectric point, hydrophilicity, mechanical properties, and structural integrity of the GelMA-based inks and 3D-printed scaffolds were thoroughly characterized to ensure their suitability for biological applications.