Article
Clinical Neurology
Stylianos Ravanidis, Anastasia Bougea, Dimitra Karampatsi, Nikolaos Papagiannakis, Matina Maniati, Leonidas Stefanis, Epaminondas Doxakis
Summary: This study identified specific circRNAs in blood mononuclear cells of patients with Parkinson's disease, which may serve as diagnostic biomarkers for PD. The circRNAs are derived from genes with essential brain functions and may hint on the PD pathways affected.
MOVEMENT DISORDERS
(2021)
Article
Medicine, Research & Experimental
Jie Zhu, Ling Han, Ruilai Liu, Zhenghua Zhang, Qiong Huang, Xu Fang, Ke Yang, Guiqin Huang, Zhizhong Zheng, Nikhil Yawalkar, Hui Deng, Kexiang Yan
Summary: By analyzing the proteome profile of PBMCs from patients with PsA, PsO, and healthy controls, this study identified 14 potential biomarkers for PsA diagnosis. Additionally, the expression of SIRT2 was significantly higher in PsA patients compared to PsO and healthy controls, and was negatively correlated with the phosphorylation of p38MAPK.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Oncology
Jacobo Rogado, Fernando Pozo, Kevin Troule, Jose Miguel Sanchez-Torres, Nuria Romero-Laorden, Rebeca Mondejar, Olga Donnay, Anabel Ballesteros, Vilma Pacheco-Barcia, Javier Aspa, Fatima Al-Shahrour, Arantzazu Alfranca, Ramon Colomer
Summary: Peripheral blood mononuclear cells (PBMCs) have been explored as potential biomarkers to predict the efficacy of anti-PD-1 immunotherapy in advanced non-small cell lung cancer (NSCLC) patients. High baseline levels of circulating T cell subpopulations related to tissue lymphocyte recruitment were found to be associated with poorer outcomes in immunotherapy-treated advanced NSCLC patients.
Article
Biochemistry & Molecular Biology
Orietta Pansarasa, Maria Garofalo, Eveljn Scarian, Francesca Dragoni, Jessica Garau, Rosalinda Di Gerlando, Luca Diamanti, Matteo Bordoni, Stella Gagliardi
Summary: This review provides an overview of peripheral blood mononuclear cells (PBMCs) as a potential source of biomarkers in amyotrophic lateral sclerosis (ALS). It focuses on altered RNA metabolism and protein abnormalities, as well as the correlation between biological parameters and disease phenotypes. Although further studies are needed, PBMCs show great potential in ALS research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemical Research Methods
Michal Alexovic, Joshua Raoul Lindner, Peter Bober, Remi Longuespee, Jan Sabo, Katarina Davalieva
Summary: In this review, recent applications of proteomic analysis of PBMCs are described and promising protein biomarker candidates are emphasized.
Article
Immunology
Santiago G. Lago, Jakub Tomasik, Geertje F. van Rees, Marina Rubey, Emiliano Gonzalez-Vioque, Jordan M. Ramsey, Frieder Haenisch, Jantine A. Broek, Javier Vazquez-Bourgon, Sergi Papiol, Paula Suarez-Pinilla, Tillmann Ruland, Bonnie Auyeug, Olya Mikova, Nikolett Kabacs, Volker Arolt, Simon Baron-Cohen, Benedicto Crespo-Facorro, Sabine Bahn
Summary: Recent evidence suggests that comorbidities between neuropsychiatric conditions and metabolic syndrome may exacerbate long-term side-effects of psychiatric medication and increase mortality. Peripheral blood cells are considered as an accessible surrogate model for metabolic alterations, facilitating stratification and prediction of pathological changes and side effects in schizophrenia patients following antipsychotic therapy.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Article
Pharmacology & Pharmacy
Vera Vorstandlechner, Dragan Copic, Katharina Klas, Martin Direder, Bahar Golabi, Christine Radtke, Hendrik J. Ankersmit, Michael Mildner
Summary: This study demonstrates the anti-fibrotic effect of PBMCsec on cutaneous scars in both mouse and human experimental settings. PBMCsec regulates the expression of fibrosis-related genes, attenuates myofibroblast differentiation, and inhibits the breakdown of elastic fibers. These findings suggest that PBMCsec could be a novel therapeutic option for treating skin scarring.
Article
Materials Science, Multidisciplinary
Byeongyeon Kim, In-Ho Seo, Gwang Myeong Seo, Woo-Joong Kim, Eui-Cheol Shin, Sungyoung Choi
Summary: The study introduces a blood divider (B-DV) as a centrifuge tube insert to prevent RBC contamination during PBMC harvest, simplifying the separation process and enabling rapid, easy PBMC isolation.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Biochemistry & Molecular Biology
Kosar Hooshmand, Glenda M. Halliday, Sandy S. Pineda, Greg T. Sutherland, Boris Guennewig
Summary: Genetic factors and pathological mechanisms play crucial roles in the early detection and treatment of neurodegenerative disorders. This study discovered that there is little overlap between AD and PD, with AD mainly characterized by changes related to nervous system development in the brain, and PD involving abnormalities in exocytosis and genes related to the SNARE complex. These findings contribute to a better understanding of the specific pathomechanisms of these two diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Belay Tessema, Janine Riemer, Ulrich Sack, Brigitte Koenig
Summary: The results of CSA parameters measured in PBMCs are influenced by the PBMC isolation method, age, seasonal variation, and gender. Researchers and physicians should properly interpret the results of CSA parameters in PBMCs by considering these factors. Separate CSA evaluation standards should be used based on the isolation method, age, gender, and season-dependent factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Keiichiro Yoshioka, Hironori Sato, Takeshi Kawasaki, Daisuke Ishii, Takuro Imamoto, Mitsuhiro Abe, Yoshinori Hasegawa, Osamu Ohara, Koichiro Tatsumi, Takuji Suzuki
Summary: RNA sequencing analysis revealed differential gene expression patterns in peripheral blood mononuclear cells (PBMCs) between patients with pulmonary sarcoidosis and healthy controls, with 270 differentially expressed genes involved in various biological processes related to the pathobiology of sarcoidosis. Enrichment analysis of gene ontology and KEGG pathways indicated the potential involvement of specific signaling pathways in sarcoidosis pathogenesis, providing insight into potential therapeutic targets for the disease.
FRONTIERS IN MEDICINE
(2022)
Article
Medicine, General & Internal
Chiung-Jung Wen, Chin-Hao Chang, Chin-Ying Chen, Jen-Kuei Peng, Hsien-Liang Huang, Pei-Ni Chuang, Ching-Yu Chen, Jaw-Shiun Tsai
Summary: The study found that the mRNA levels of ICAM-1 and TLR4 in PBMCs are significantly higher in older adults compared to younger adults. TLR4 is an independent factor affecting the expression of ICAM-1 in PBMCs. This suggests that ICAM-1 and TLR4 in PBMCs could be potential biomarkers for the ageing process.
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Endocrinology & Metabolism
Mehmet M. Altintas, Salvatore DiBartolo, Lana Tadros, Beata Samelko, Haimanot Wasse
Summary: This study aimed to elucidate mitochondrial function and associated stress pathologies in kidney disease using peripheral blood mononuclear cells (PBMCs) of hemodialysis patients. The analysis revealed a significant reduction in mitochondrial respiration in ESKD patient PBMCs, correlated with changes in key bioenergetic parameters and protein expression levels. The findings suggest a link between mitochondrial dysfunction and ESKD, with potential therapeutic implications.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Medicine, General & Internal
Vincent M. Tutino, Haley R. Zebraski, Hamidreza Rajabzadeh-Oghaz, Muhammad Waqas, James N. Jarvis, Konrad Bach, Maxim Mokin, Kenneth V. Snyder, Adnan H. Siddiqui, Kerry E. Poppenberg
Summary: PBMCs play a crucial role in the inflammation associated with intracranial aneurysm (IA) pathophysiology. By analyzing PBMC RNA from patients with and without IAs, researchers identified a signature of 54 genes associated with IA, which could potentially serve as blood-based biomarkers for the disease. Further bioinformatics analyses highlighted key processes and pathways involved in IA pathophysiology, and correlation analysis revealed certain genes associated with rupture risk, indicating their potential as predictive biomarkers.
Article
Veterinary Sciences
Seo-Young Hwang, Ju-Hyun An, Kyung-Bo Kim, Jeong-Hwa Lee, Su-Min Park, Ye-In Oh, Hyung Kyu Chae, Hwa-Young Youn
Summary: This study evaluated the role of PBMCs in obesity-related chronic inflammation and found that the gene expression levels of inflammation-related adipokines and pro-inflammatory cytokines were altered in PBMCs, potentially contributing to the low-grade chronic inflammation observed in obesity.
VETERINARY MEDICINE AND SCIENCE
(2022)