Article
Biochemistry & Molecular Biology
Tingting Shen, Ning Jia, Shanshan Wei, Wenyan Xu, Tingting Lv, Jiaoteng Bai, Bing Li
Summary: The mitochondria-localized heat shock protein mtHSC70-1 in Arabidopsis plays a crucial role in regulating root development and growth through the modulation of reactive oxygen species homeostasis and polar auxin transport.
Article
Plant Sciences
Taras Pasternak, Ivan A. Paponov, Serhii Kondratenko
Summary: Procedures have been optimized for direct regeneration of entire plants from shoot and root protoplasts of Arabidopsis thaliana, including adjustments to culture media, detection protocols, and optimization of cell reprogramming. Cell division competence was detected through DNA replication events and high cell density, and further optimized by applying glutathione to increase sensitivity to cell cycle activation by auxin. The developed protocol allows investigation into the molecular mechanism of somatic plant cell de-differentiation.
Article
Plant Sciences
Sapana Nongmaithem, Rachana Ponukumatla, Yellamaraju Sreelakshmi, Pierre Frasse, Mondher Bouzayen, Rameshwar Sharma
Summary: Our study reveals a connection between polar auxin transport (PAT) and glutathione levels in tomato similar to Arabidopsis. Furthermore, TIBA rescues the short root phenotype of the pct1-2 mutant by acting on a PAT component distinct from the site of action of other PAT inhibitors.
JOURNAL OF PLANT GROWTH REGULATION
(2021)
Article
Biochemistry & Molecular Biology
Meng Wang, Panpan Li, Yao Ma, Xiang Nie, Markus Grebe, Shuzhen Men
Summary: The composition of plant membrane sterols has been found to antagonistically affect auxin biosynthesis, thus influencing plant growth and gravitropism. This study sheds light on a previously unexplored sterol-dependent modulation of auxin biosynthesis during root elongation in Arabidopsis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Haiyue Ai, Julia Bellstaedt, Kai Steffen Bartusch, Lennart Eschen-Lippold, Steve Babben, Gerd Ulrich Balcke, Alain Tissier, Bettina Hause, Tonni Grube Andersen, Carolin Delker, Marcel Quint
Summary: Roots are able to sense and respond to elevated temperature independently of shoot-derived signals. This response is mediated by an unknown root thermosensor that uses auxin as a messenger to relay temperature signals to the cell cycle. Growth promotion is primarily achieved by increasing cell division rates in the root apical meristem, dependent on de novo local auxin biosynthesis and temperature-sensitive organization of the polar auxin transport system.
Article
Biochemistry & Molecular Biology
Michael Stitz, David Kuster, Maximilian Reinert, Mikhail Schepetilnikov, Beatrice Berthet, Jazmin Reyes-Hernandez, Denis Janocha, Anthony Artins, Marc Boix, Rossana Henriques, Anne Pfeiffer, Jan Lohmann, Emmanuel Gaquerel, Alexis Maizel
Summary: Plant organogenesis requires the matching of metabolic resources with developmental programs. The root system in Arabidopsis is determined by primary root-derived lateral roots (LRs) and adventitious roots (ARs) formed from non-root organs. Lateral root formation requires the activation of transcription factors ARF7, ARF19, and LBD16. Adventitious root formation relies on the activation of LBD16 by auxin and WOX11. The allocation of shoot-derived sugar to the roots affects branching, but the mechanism of LRs formation is still unknown.
Article
Plant Sciences
Huabin Liu, Qiong Luo, Chao Tan, Jia Song, Tan Zhang, Shuzhen Men
Summary: Auxin plays an important role in the coordinated development of the embryo, endosperm, and seed coat during seed development. In this study, the distribution pattern and function of auxin during Arabidopsis seed development were investigated. It was found that auxin response signals exhibited a dynamic distribution pattern, with strong signals observed at specific locations in the seed coat. Auxin biosynthesis genes and auxin carriers were identified as contributing factors to the accumulation and polar distribution of auxin in the seed coat. Furthermore, the accumulation of auxin in the seed coat was found to regulate seed size. These findings provide valuable insights and tools for improving crop yields.
Article
Plant Sciences
Kacper Dziewit, Ales Pencik, Katarzyna Dobrzynska, Ondrej Novak, Bozena Szal, Anna Podgorska
Summary: NH4+-mediated accelerated auxin turnover rates lead to transient and local IAA peaks. The distinct auxin pattern in tissues is correlated with the short and highly branched root system adaptations of NH4+-grown plants. The spatiotemporal distribution of auxin may act as a root-shaping signal specific for adapting to NH4+ stress conditions.
Article
Biochemistry & Molecular Biology
Houjun Zhou, Haiman Ge, Jiahong Chen, Xueqin Li, Lei Yang, Hongxia Zhang, Yuan Wang
Summary: This study reveals the mechanism by which high concentration of SA affects gravitropic root growth and root hair development in plants. The regulation of PIN2 gene transcription and endocytosis of PIN2 protein are involved in this process. Exogenous SA application inhibits gravitropic root growth and root hair development by affecting auxin accumulation and PIN2 distribution.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ana Smolko, Natasa Bauer, Iva Pavlovic, Ales Pencik, Ondrej Novak, Branka Salopek-Sondi
Summary: Salt and osmotic stress have significant effects on the auxin metabolome and distribution, leading to changes in gene expression levels. Long-term salt stress resulted in stable auxin metabolites but altered distribution and gene expression profiles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Developmental Biology
Yingzhang Huang, Limeng Zhou, Chunhui Hou, Dianjing Guo
Summary: This study reveals the proteome landscape of Arabidopsis early embryogenesis through nanoproteomic analysis, and identifies gene enrichment related to ribosome biogenesis and auxin signaling. The positive correlation between mRNA and protein is confirmed by integrating RNA-sequencing data. This work provides valuable insights for further investigation into plant embryo development.
Article
Plant Sciences
Jiehua Wang, Muhammad Moeen-ud-din, Shaohui Yang
Summary: Zinc has dose-dependent effects on both vegetative and reproductive growth of Arabidopsis, regulated by differences in auxin synthesis and transport. Exogenous IAA can alleviate zinc-induced short-root phenotype by affecting auxin accumulation, while mutants with deficient auxin mechanisms exhibit hypersensitivity to zinc.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Huirui Guan, Xin Liu, Yanping Fu, Xiaomin Han, Yanli Wang, Qing Li, Liang Guo, Luis A. J. Mur, Yahui Wei, Wei He
Summary: In this study, physiological and genetic analyses were conducted to show that A. oxytropis promotes growth and development in plants by regulating auxin, suggesting a possible role in benefiting its locoweed hosts independent of its toxin production.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Xudong Zhang, Xing Huang, Yan Li, Faqing Tao, Qinshi Zhao, Weiqi Li
Summary: The study found that flavonoids with a 6-hydroxyl group, unlike known flavonoid-PAT inhibitors, can promote polar auxin transport in roots, leading to increased root length and changes in root tissue structure. In contrast, flavonoids without a 6-hydroxyl group may have opposite or no effects on root growth.
Article
Plant Sciences
Omid Karami, Cheryl Philipsen, Arezoo Rahimi, Annisa Ratna Nurillah, Kim Boutilier, Remko Offringa
Summary: The study reveals that endogenous auxin is crucial for maintaining embryonic cell identity and the formation of multicellular proembryos during somatic embryogenesis induced by 2,4-D and AHL15 in Arabidopsis.
Article
Biochemistry & Molecular Biology
Abu Imran Baba, Gabor Rigo, Ferhan Ayaydin, Ateeq Ur Rehman, Norbert Andrasi, Laura Zsigmond, Ildiko Valkai, Janos Urbancsok, Imre Vass, Taras Pasternak, Klaus Palme, Laszlo Szabados, Agnes Cseplo
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Plant Sciences
Norbert Andrasi, Gabor Rigo, Laura Zsigmond, Imma Perez-Salamo, Csaba Papdi, Eva Klement, Aladar Pettko-Szandtner, Abu Imran Baba, Ferhan Ayaydin, Ramakrishna Dasari, Agnes Cseplo, Laszlo Szabados
JOURNAL OF EXPERIMENTAL BOTANY
(2019)
Article
Biochemistry & Molecular Biology
Abu Imran Baba, Norbert Andrasi, Ildiko Valkai, Terez Gorcsa, Lilla Koczka, Zsuzsanna Darula, Katalin F. Medzihradszky, Laszlo Szabados, Attila Feher, Gabor Rigo, Agnes Cseplo
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Plant Sciences
Hajnalka Kovacs, David Aleksza, Abu Imran Baba, Anita Hajdu, Anna Maria Kiraly, Laura Zsigmond, Szilvia Z. Toth, Laszlo Kozma-Bognar, Laszlo Szabados
FRONTIERS IN PLANT SCIENCE
(2019)
Article
Biochemistry & Molecular Biology
Agnes Cseplo, Laura Zsigmond, Norbert Andrasi, Abu Imran Baba, Nitin M. Labhane, Andrea Peto, Zsuzsanna Kolbert, Hajnalka E. Kovacs, Gabor Steinbach, Laszlo Szabados, Attila Feher, Gabor Rigo
Summary: The study reveals that Arabidopsis AtCRK5 protein kinase is involved in establishing the auxin gradient at root tips, and the delayed gravitropic response in the Atcrk5-1 mutant is associated with oxidative stress and nitric oxide levels. Treatment with oxidative stress inducers or H2O2 can partially restore proper auxin distribution.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Abu Imran Baba, Mohd Yaqub Mir, Riyazuddin Riyazuddin, Agnes Cseplo, Gabor Rigo, Attila Feher
Summary: Plants are essential for a balanced life on Earth, providing food and oxygen. The study of plants in space exploration can contribute to the development of life support technologies for long-term space missions. However, our knowledge about how plants sense and adapt to microgravity in space is limited. Investigating the ability of plants to survive in microgravity is important for providing food for astronauts and understanding plant adaptation to unique environments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)