Article
Oncology
Danfang Wang, Jianhui Li, Wenhan Li
Summary: This study investigates the role of LHPP in gastric cancer (GC) and finds that LHPP acts as a tumor suppressor gene by inhibiting proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) of GC cells. The underlying mechanism involves regulating the PI3K/AKT/mTOR pathway.
Review
Biochemistry & Molecular Biology
Antonino Glaviano, Aaron S. C. Foo, Hiu Y. Lam, Kenneth C. H. Yap, William Jacot, Robert H. Jones, Huiyan Eng, Madhumathy G. Nair, Pooyan Makvandi, Birgit Geoerger, Matthew H. Kulke, Richard D. Baird, Jyothi S. Prabhu, Daniela Carbone, Camilla Pecoraro, Daniel B. L. Teh, Gautam Sethi, Vincenzo Cavalieri, Kevin H. Lin, Nathalie R. Javidi-Sharifi, Eneda Toska, Matthew S. Davids, Jennifer R. Brown, Patrizia Diana, Justin Stebbing, David A. Fruman, Alan P. Kumar
Summary: The PI3K/AKT/mTOR (PAM) signaling pathway plays a crucial role in cell survival, growth, and cell cycle progression. Dysregulation of this pathway is associated with cancer development and resistance to therapy. This review focuses on the major dysregulations in the PAM signaling pathway in cancer and discusses strategies for overcoming treatment resistance. The role of PAM signaling in immunology and immunotherapies is also discussed.
Review
Oncology
Wojciech Wiese, Julia Barczuk, Olga Racinska, Natalia Siwecka, Wioletta Rozpedek-Kaminska, Artur Slupianek, Radoslaw Sierpinski, Ireneusz Majsterek
Summary: The PI3K/Akt/mTOR pathway is crucial in cancer development and can be a potential therapeutic target for leukemia. Recent research indicates that inhibition of this pathway may lead to improved treatment outcomes for leukemia.
Article
Medicine, Research & Experimental
Jie Yao, Zefen Wang, Yong Cheng, Chao Ma, Yahua Zhong, Yilei Xiao, Xu Gao, Zhiqiang Li
Summary: This study found that exosomal miR-15a and miR-92a derived from M2 macrophages inhibit cell migration and invasion of glioma cells by activating the PI3K/AKT/mTOR signaling pathway.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Oncology
Qianxu Jin, Jiahui Zhao, Zijun Zhao, Shiyang Zhang, Zhimin Sun, Yunpeng Shi, Hongshan Yan, Yizheng Wang, Liping Liu, Zongmao Zhao
Summary: CAMK1D is expressed at lower levels in glioma tissues and is positively correlated with the WHO classification of gliomas. Knockdown of CAMK1D promotes the proliferation, migration, and invasion of glioma cells, while overexpression has the opposite effect. Mechanistically, CAMK1D regulates glioma cell function through the PI3K/AKT/mTOR pathway. These findings suggest that CAMK1D may serve as a prognostic predictor and a potential therapeutic target for glioma.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lan Ma, Ruixue Zhang, Demin Li, Tingting Qiao, Xiaoying Guo
Summary: Fluoride inhibits chondrocyte proliferation and promotes autophagy through the PI3K/AKT/mTOR signaling pathway in chondrogenesis. The study also showed that fluoride downregulates the mTOR signaling pathway-related genes and is associated with changes in autophagy-related gene expressions. MHY1485, a small-molecular mTOR activator, can reverse the fluoride-induced promotion of autophagy and recover the downregulation of proliferative chondrocyte markers induced by fluoride in chondrogenic cells.
CHEMICO-BIOLOGICAL INTERACTIONS
(2021)
Article
Medicine, Research & Experimental
Maryam Akbarzadeh, Ainaz Mihanfar, Shabnam Akbarzadeh, Bahman Yousefi, Maryam Majidinia
Summary: The PI3K/AKT/mTOR signaling pathway plays a crucial role in cancer and has been found to interact with miRNAs, which is important for understanding cancer biology.
Review
Oncology
Huayi Li, Lorenzo Prever, Emilio Hirsch, Federico Gulluni
Summary: The PI3K signaling pathway is crucial in breast cancer and inhibitors targeting this pathway show promising activity, but resistance and adverse reactions limit their efficacy. Combination therapies and identifying suitable patient subpopulations are needed to enhance therapeutic benefit.
Review
Pharmacology & Pharmacy
Amin Iranpanah, Leila Kooshki, Seyed Zachariah Moradi, Luciano Saso, Sajad Fakhri, Haroon Khan
Summary: As a major public health concern, neurological diseases and neurodegenerative diseases significantly impact the growing aging population, leading to disability and mortality. Apoptosis, inflammation, and oxidative stress play crucial roles in the development of neurodegenerative diseases, with the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) pathway being a key player. The blood-brain barrier poses challenges for drug delivery, but exosomes have emerged as potential nano-sized vehicles for central nervous system drug delivery due to their ability to cross the blood-brain barrier and their unique characteristics such as low immunogenicity and great tissue/cell penetration capabilities. This systematic review highlights the therapeutic potential of exosomes targeting the PI3K/Akt/mTOR signaling pathway in the context of neurodegenerative diseases and neurological diseases.
Review
Pharmacology & Pharmacy
Ayda Baghery Saghchy Khorasani, Atieh Pourbagheri-Sigaroodi, Ali Pirsalehi, Ava Safaroghli-azar, Mohammad Reza Zali, Davood Bashash
Summary: Genetic and epigenetic alterations have long been the focus of cancer pathogenesis research, with dysregulated genes and protein products potentially affecting cancer cell growth through signaling cascades regulating cell survival and metabolism. The activation of the phosphatidylinositol-3-kinase (PI3K) axis in about one-third of human cancers, including gastric cancer, plays a significant role in the development of malignancies. This indicates the potential importance of targeting this axis in therapeutic strategies for such cancers.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Plant Sciences
Dan Qiao, Jian Xing, Yunxiao Duan, Shiyu Wang, Guangyuan Yao, Shengjun Zhang, Jingchun Jin, Zhenhua Lin, Liyan Chen, Yingshi Piao
Summary: Baicalein inhibits the progression of gastric cancer by regulating the miR-7/FAK/AKT signaling pathway, leading to the suppression of proliferation, metastasis, and angiogenesis in gastric cancer cells.
Article
Pharmacology & Pharmacy
Yunhao Ma, Hao Xu, Zhongkun Zhou, Yanan Tian, Kangjia Du, Hao Zhang, Xinrong Jiang, Juan Lu, Yuqing Niu, Lixue Tu, Huanxiang Liu, Hongmei Zhu, Peng Chen, Yingqian Liu
Summary: In the treatment of gastric cancer, natural products modified by structural modification can be a promising strategy for finding anti-gastric cancer drugs. In this study, a novel derivative CFNC was synthesized and found to have potential anti-gastric cancer effects by regulating the PI3K/AKT/mTOR signaling pathway.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2023)
Article
Medicine, Research & Experimental
Yong Zhong, Fan Yu, Ling Yang, Yu Wang, Lin Liu, Chengyou Jia, Haidong Cai, Jianshe Yang, Shiyang Sheng, Zhongwei Lv, Li Weng, Bo Wu, Xiaoping Zhang
Summary: This study found that HOXD9 is upregulated in ATC and its knockdown can inhibit proliferation, migration, invasion, and EMT, while increasing apoptosis in ATC cells. Additionally, HOXD9 may be involved in ATC progression by regulating miR-451a and PSMB8.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Plant Sciences
Sajad Fakhri, Amin Iranpanah, Mohammad Mehdi Gravandi, Seyed Zachariah Moradi, Mohammad Ranjbari, Mohammad Bagher Majnooni, Javier Echeverria, Yaping Qi, Mingfu Wang, Pan Liao, Mohammad Hosein Farzaei, Jianbo Xiao
Summary: Natural products derived from plants exhibit promising potential in managing and treating various neuronal disorders by targeting the PI3K/Akt/mTOR signaling pathway. Recent studies have highlighted the multi-target nature of these compounds, showcasing their diverse pharmacological and biological activities for combating neurodegenerative diseases effectively. The exploration of plant-derived secondary metabolites in neuroprotection through the PI3K/Akt/mTOR pathway represents an innovative strategy for developing novel therapeutic agents.
Article
Cell Biology
Hui Li, Shufen Zhao, Liwei Shen, Peige Wang, Shihai Liu, Yingji Ma, Zhiwei Liang, Gongjun Wang, Jing Lv, Wensheng Qiu
Summary: The study revealed that high expression of E2F2 in gastric cancer is associated with poor prognosis, and it promotes cancer cell migration and invasion by inhibiting autophagy through the PI3K/Akt/mTOR pathway. E2F2 may also modulate tumor immunity.
Article
Gastroenterology & Hepatology
Stephan Buch, Hamish Innes, Philipp Ludwig Lutz, Hans Dieter Nischalke, Jens U. Marquardt, Janett Fischer, Karl Heinz Weiss, Jonas Rosendahl, Astrid Marot, Marcin Krawczyk, Markus Casper, Frank Lammert, Florian Eyer, Arndt Vogel, Silke Marhenke, Johann von Felden, Rohini Sharma, Stephen Rahul Atkinson, Andrew McQuillin, Jacob Nattermann, Clemens Schafmayer, Andre Franke, Christian Strassburg, Marcella Rietschel, Heidi Altmann, Stefan Sulk, Veera Raghavan Thangapandi, Mario Brosch, Carolin Lackner, Rudolf E. Stauber, Ali Canbay, Alexander Link, Thomas Reiberger, Mattias Mandorfer, Georg Semmler, Bernhard Scheiner, Christian Datz, Stefano Romeo, Stefano Ginanni Corradini, William Lucien Irving, Joanne R. Morling, Indra Neil Guha, Eleanor Barnes, M. Azim Ansari, Jocelyn Quistrebert, Luca Valenti, Sacha A. Mueller, Marsha Yvonne Morgan, Jean-Francois Dufour, Jonel Trebicka, Thomas Berg, Pierre Deltenre, Sebastian Mueller, Jochen Hampe, Felix Stickel
Summary: This study identifies rs2242652 in TERT as a novel protective factor for HCC in patients with alcohol-related cirrhosis.
Article
Chemistry, Multidisciplinary
Ruta Palepsiene, Martynas Maciulevicius, Paulius Ruzgys, Baltramiejus Jakstys, Saulius Satkauskas
Summary: Gene electrotransfer (GET) is a promising technique for gene therapy, but its success rate is influenced by the understanding of electroporation mechanism and other factors. This study analyzed the impact of low concentrations of Ca2+ on DNA electrotransfer and found that even 0.25 mM CaCl2 can significantly decrease the efficiency of GET and cell viability. The observed decrease in pDNA electrotransfer was not due to cell death but secondary mechanisms. The findings highlight the importance of Ca2+ in gene electrotransfer and its potential application in vivo.
APPLIED SCIENCES-BASEL
(2023)
Article
Gastroenterology & Hepatology
Katrin Bose, Florian G. Scurt, Cosima Thon, Sabine Franke, Christian Schulz, Peter Malfertheiner, Alexander Link
Summary: This study aimed to evaluate DNA methylation as a diagnostic tool for differentiating between benign and malignant ascites. The results showed that methylation levels were associated with ascites in patients with tumors, but not in patients without tumors. In addition, inflammatory cells were also found to be related to DNA methylation changes.
JOURNAL OF GASTROINTESTINAL AND LIVER DISEASES
(2023)
Article
Engineering, Environmental
Cristina Povedano-Priego, Fadwa Jroundi, Pier L. Solari, Isabel Guerra-Tschuschke, Maria del Mar Abad-Ortega, Alexander Link, Ramiro Vilchez-Vargas, Mohamed L. Merroun
Summary: In this study, the impact of bentonite microbial communities on the allotropic transformation of Se(IV) bioreduction products under DGR relevant conditions was investigated. Moreover, the Se amendment-dependent shifts in the bentonite microbial populations were assessed. The results showed that the bentonite color changed from orange to black after selenite treatment, and the allotropic changes of Se bioreduction products were tracked using analytical techniques. The distribution of strains involved in Se(IV) reduction and Se(0) allotropic biotransformation potential was identified.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Gastroenterology & Hepatology
Darja Nikitina, Konrad Lehr, Ramiro Vilchez-Vargas, Laimas Virginijus Jonaitis, Mindaugas Urba, Juozas Kupcinskas, Jurgita Skieceviciene, Alexander Link
Summary: This study characterized the microbiota of gastric cancer using 16S rRNA gene and its transcript and identified differences in bacterial composition. The results showed that there was only partial overlap between DNA and RNA samples and differences between gastric cancer patients and healthy controls depending on the chosen sequencing modality. Furthermore, RNA sequencing was more sensitive for detecting differences in bacterial richness and specific bacteria. Overall, the choice of sequencing modality affects the characterization of the microbiota and the differences between case and control.
WORLD JOURNAL OF GASTROENTEROLOGY
(2023)
Article
Gastroenterology & Hepatology
Andreas Stallmach, Lutz von Mueller, Martin Storr, Alexander Link, Peter C. C. Konturek, Philipp Christoph Solbach, Karl Heinz Weiss, Steffen Wahler, Maria J. G. T. Vehreschild
Summary: The frequency of FMT use in Germany is lower compared to other European countries. One of the obstacles is the regulatory classification of FMT as a non-approved drug, resulting in higher costs and reimbursement difficulties. The proposed regulation by the European Commission to classify FMT as a transplant may change the regulatory situation in Germany and promote nationwide availability of this recommended therapeutic procedure.
ZEITSCHRIFT FUR GASTROENTEROLOGIE
(2023)
Article
Gastroenterology & Hepatology
Wolfgang Fischbach, Jan Bornschein, Jorg C. Hoffmann, Sibylle Koletzko, Alexander Link, Lukas Macke, Peter Malfertheiner, Kerstin Schuette, Dieter-Michael Selgrad, Sebastian Suerbaum, Christian Schulz
ZEITSCHRIFT FUR GASTROENTEROLOGIE
(2023)
Article
Microbiology
Baptiste Oosterlinck, Hannah Ceuleers, Wout Arras, Joris G. De Man, Karen Geboes, Heiko De Schepper, Marc Peeters, Sarah Lebeer, Jurgita Skieceviciene, Georgina L. Hold, Juozas Kupcinskas, Alexander Link, Benedicte Y. De Winter, Annemieke Smet
Summary: This study identified the mucin-microbiome signatures in gastric adenocarcinomas and their impact on clinical outcomes. The findings showed that intestinal mucin phenotype and high-level MUC13 expression were associated with poor survival, while gastric MUC5AC or MUC6 abundance was associated with a more favorable outcome. Furthermore, oral bacteria such as Neisseria, Prevotella, and Veillonella were found to play a potential role in MUC13 signaling and were associated with intestinal and mixed mucin phenotype tumors.
Article
Pharmacology & Pharmacy
Martynas Maciulevicius, Renaldas Raisutis, Baltramiejus Jakstys, Linas Svilainis, Andrius Chaziachmetovas, Saulius Satkauskas
Summary: Microbubble and ultrasound facilitated intracellular Ca2+ delivery, known as sonoporation, offers a promising alternative to conventional chemotherapy due to its spatio-temporally controllable and side-effect-free nature. This study demonstrates that a 5 mM concentration of Ca2+ in combination with ultrasound or ultrasound and Sonovue microbubbles can be used as an alternative to the anticancer drug bleomycin. Ca2+ delivery via sonoporation induces cell death in Chinese hamster ovary cells, similar to the combination of bleomycin and sonoporation, without causing systemic toxicity. Furthermore, Ca2+ delivery via sonoporation alters vital characteristics of viable cells and elicits sudden cell death within a short period of time.
Article
Plant Sciences
Lina Jociene, Edvina Krokaite, Tomas Rekasius, Ramunas Vilcinskas, Asta Judzentiene, Vitas Marozas, Eugenija Kupcinskiene
Summary: In this study, the ionomic parameters of Juniperus communis needles in different habitats in Lithuania were analyzed. The concentrations of elements in the needles varied significantly between contrasting populations, and the concentrations of main nutritional elements were lower in transition mires and quaking bogs.
Article
Medicine, General & Internal
Jastin Link, Cosima Thon, Vytenis Petkevicius, Ruta Steponaitiene, Peter Malfertheiner, Juozas Kupcinskas, Alexander Link
Summary: This study aimed to evaluate the clinical relevance of xeno-miRNA miR-168 in the gastric mucosa during preneoplastic conditions and gastric carcinogenesis. The results showed that miR-168 levels were higher in patients with moderate and severe intestinal metaplasia, suggesting its potential involvement in mucosal lesions and gastric cancer development.
Article
Endocrinology & Metabolism
Francisco J. Osuna-Prieto, Huiwen Xu, Lourdes Ortiz-Alvarez, Xinyu Di, Isabelle Kohler, Lucas Jurado-Fasoli, Jose Rubio-Lopez, Julio Plaza-Diaz, Ramiro Vilchez-Vargas, Alexander Link, Angel Gil, Jonatan R. Ruiz, Patrick C. N. Rensen, Borja Martinez-Tellez
Summary: The study investigated the relationship between fecal microbiota diversity and composition and plasma bile acid levels in young adults. The results showed that fecal microbiota diversity and evenness, as well as the relative abundance of Firmicutes and Bacteroidetes phyla, were positively associated with plasma levels of secondary bile acids. However, further investigations are needed to confirm whether gut microbiota composition can regulate plasma bile acid concentrations in humans.
Article
Chemistry, Multidisciplinary
Aivaras Salasevicius, Dovile Uzdavinyte, Mindaugas Visockis, Paulius Ruzgys, Saulius Satkauskas
Summary: In this study, the influence of pulsed electric field (PEF) processing on the nutritional properties of raw milk was evaluated and compared to conventional thermal pasteurization methods. It was found that PEF treatment significantly reduced the content of beta-lactoglobulin in liquid whey samples. However, all pasteurization methods had minimal impact on the concentrations of vitamins, amino acids, cholesterol, and fatty acids. Additionally, thermal pasteurization effectively eliminated bacterial colonies.
APPLIED SCIENCES-BASEL
(2023)