期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 21, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/ijms21010042
关键词
PH and NNMS; BSA; QTL; haplotype analysis; soybean
资金
- National Nature Science Foundation of China [31771882, 31971899]
- Natural Science Foundation of Heilongjiang [C2018017]
- Jilin Province Agricultural Science and Technology Innovation Project [C92070420]
- subproject of National Key RD Program [2016YFD0100201-21]
- Nature Science Fund for Distinguished Young Scholars of Heilongjiang Province [JC2017006, JC2016004]
Soybean is one of the most important food and oil crops in the world. Plant height (PH) and the number of nodes on the main stem (NNMS) are quantitative traits closely related to soybean yield. In this study, we used 208 chromosome segment substitution lines (CSSL) populations constructed using SN14 and ZYD00006 for quantitative trait locus (QTL) mapping of PH and NNMS. Combined with bulked segregant analysis (BSA) by extreme materials, 8 consistent QTLs were identified. According to the gene annotation of the QTL interval, a total of 335 genes were obtained. Five of which were associated with PH and NNMS, potentially representing candidate genes. RT-qPCR of these 5 candidate genes revealed two genes with differential relative expression levels in the stems of different materials. Haplotype analysis showed that different single nucleotide polymorphisms (SNPs) between the excellent haplotypes in Glyma.04G251900 and Glyma.16G156700 may be the cause of changes in these traits. These results provide the basis for research on candidate genes and marker-assisted selection (MAS) in soybean breeding.
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