Article
Environmental Sciences
Patricia Aline Bressiani, Geiciane Locatelli Alves, Inara Giacobbo de Marco, Mariana Tonello Biffi, Sabrina Ishikawa, Fernando Cesar Manosso, Eduardo Michel Vieira Gomes, Ticiane Sauer Pokrywiecki, Ana Paula de Oliveira Schmitz, Elisangela Dusman
Summary: This study aimed to investigate the effects of agrochemical exposure on cytotoxicity and mutagenicity using a buccal micronucleus cytome assay in a population from Vila Rural ' Agua Viva, Brazil. The results showed statistically significant differences between the control group and the exposed population. Smoking, physical activity, age, and duration of exposure did not have a linear relationship with the frequencies of cytotoxicity and mutagenicity. Additionally, the presence of 2,4-D herbicide was detected in the community's water samples.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Tereza Cervena, Andrea Rossnerova, Tana Zavodna, Jitka Sikorova, Kristyna Vrbova, Alena Milcova, Jan Topinka, Pavel Rossner
Summary: The evaluation of genotoxicity induced by nanomaterials in bronchial epithelial cells using different variants of the micronuclei assay showed that the DNA damage level is affected by the selected methodological approach. Transmission electron microscopy demonstrated the impact of cytochalasin-B on the transportation of NMs into cells, while dynamic light scattering measurement revealed the behavior of NMs in culture media. The genotoxic potential of various TiO2 and Ag particles differed, with the application of cytochalasin-B tending to increase the percentage of aberrant cells.
Article
Plant Sciences
Ioanna Efthimiou, Dimitris Vlastos, Vassilios Triantafyllidis, Antonios Eleftherianos, Maria Antonopoulou
Summary: This study investigated the genotoxic and cytotoxic activity of Betula pendula leaf extract and found that it exhibited antigenotoxic potential. The mixture of the extract with mitomycin-C showed significant antigenotoxic activity at low concentrations.
Article
Chemistry, Physical
S. Garcia-Carpintero, V. Jehova Gonzalez, J. Frontinan-Rubio, A. Esteban-Arranz, E. Vazquez, M. Duran-Prado
Summary: In recent years, the genotoxicity of graphene-related materials (GRMs) and other 2D materials has been evaluated using different models. The OECD TG 487 in vitro micronucleus (MNu) test is commonly used to assess the genotoxicity of nanomaterials (NMs). However, the nature of NMs interferes with the standard MNu process, requiring adaptations. The study proposes a modified in vitro MNu test with improvements to accurately test the genotoxicity of NMs, demonstrating risks associated with graphene oxide (GO) and boron nitride (hBN).
Review
Environmental Sciences
Ingra Tais Malacarne, Daniel Vitor De Souza, Maria Esther Suarez Alpire, Ana Carolina Flygare Souza, Ana Claudia Muniz Renno, Daniel Araki Ribeiro
Summary: This review evaluated the micronucleus assay in oral exfoliated cells as a tool for biomonitoring children exposed to environmental pollutants, finding conflicting results on the cytogenetic damage induced by environmental pollutants in oral cells.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Environmental Sciences
Milda Babonaite, Matas Cepulis, Jurate Kazlauskaite, Juozas Rimantas Lazutka
Summary: According to PlasticEurope, global plastics production reached 390.7 million tons in 2021. Unfortunately, a large portion of these plastics end up as waste in the ocean or on land due to their non-biodegradable nature. This study focused on evaluating the internalization rates, cytotoxicity, and genotoxicity of polystyrene nanoparticles (PS-NPs) in human peripheral blood mononuclear cells (PBMCs) in vitro. The results showed that while PS-NPs did not have a cytotoxic effect on PBMCs, they did induce primary DNA damage and micronucleated cells, indicating their genotoxic potential.
Article
Pharmacology & Pharmacy
Raffaella di Vito, Mattia Acito, Cristina Fatigoni, Carl H. Schiesser, Michael J. Davies, Francesca Mangiavacchi, Milena Villarini, Claudio Santi, Massimo Moretti
Summary: This study evaluated the cytotoxic and genotoxic potential of SeTal in liver cells through in vitro experiments. The results showed that SeTal had minimal impact on cell viability and did not cause clastogenic or aneugenic activity. These findings suggest that SeTal may be a potential active pharmaceutical ingredient.
Article
Food Science & Technology
Miroslav Misik, Armen Nersesyan, Michael Kment, Benjamin Ernst, Tahereh Setayesh, Franziska Ferk, Klaus Holzmann, Georg Krupitza, Siegfried Knasmueller
Summary: The micronucleus (MN) test using Huh6 cells shows promising results with a sensitivity of 80% and a specificity of 94% for the detection of genotoxic carcinogens. The validity of MN tests with Huh6 is higher than that of other experimental models, which suggests a potential reduction in the use of laboratory rodents in genetic toxicology studies.
FOOD AND CHEMICAL TOXICOLOGY
(2021)
Article
Environmental Sciences
Claudia Galeano-Paez, Pedro Espitia-Perez, Luisa Jimenez-Vidal, Karina Pastor-Sierra, Shirley Salcedo-Arteaga, Luz Stella Hoyos-Giraldo, Adriana Gioda, Tatiana Dillenburg Saint'Pierre, Solange Cristina Garcia, Hugo Brango, Lyda Espitia-Perez
Summary: The study found that residents of La Mojana region showed significant increase in cytogenetic damage and total Hg levels in hair, which were related to high fish consumption. High fish consumption and T-Hg contents were also correlated with necrotic and apoptotic cell frequencies. Additionally, exposure to Hg induced genetic instability and increased micronuclei frequencies in individuals who consumed certain fish species with carnivorous habits.
Article
Plant Sciences
Debora Elisa Antunes de Mendonca, Mariane Aparecida Franco de Godoy, Naiara Cristina Lucredi, Jurandir Fernando Comar, Igor Vivian Almeida, Veronica Elisa Pimenta Vicentini
Summary: This study evaluated the effects of Ganoderma lucidum fruiting body dry extract (GLE) on human liver and kidney tumor cells as well as peripheral blood lymphocytes. The results showed that low concentrations of GLE increased lymphocyte viability while high concentrations were toxic to lymphocytes. On the other hand, low concentrations of GLE exhibited cytotoxic activity and induced DNA damage in tumor cells. Therefore, G. lucidum has potential antitumor effects and could be developed into a therapeutic agent for cancer.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Environmental Sciences
Ilana Struys, Eline Verscheure, Liesbeth Lenaerts, Frederic Amant, Lode Godderis, Manosij Ghosh
Summary: Due to the genotoxic consequences of antineoplastic agents on non-cancerous tissue, it is crucial to understand their effects. This study aimed to characterize the genotoxic profile of different classes of antineoplastic drugs using a cell line assay. The results showed increased micronucleus formation with all tested drugs, as well as specific abnormalities for each drug class. These findings can aid in interpreting genotoxic aberrations in patients treated with these drugs.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2023)
Article
Chemistry, Medicinal
Zeineb Marzougui, Sylvie Huet, Anne-Louise Blier, Ludovic Le Hegarat, Haifa Tounsi-Kettiti, Riadh Kharrat, Riadh Marrouchi, Valerie Fessard
Summary: In this study, the in vivo genotoxic effects of C17-sphinganine analog mycotoxin (C17-SAMT) were evaluated using comet assays and micronucleus assay. The results showed that C17-SAMT induced DNA damage in the duodenum and spleen, but had no significant effect on chromosome and genome damage in the bone marrow. Microscopic observations of the liver revealed increased mitosis and hepatocytes' cytoplasm clarification.
Article
Environmental Sciences
Ronan Adler Tavella, Caroline Lopes Feijo Fernandes, Lucia Emanueli Schimith, Lisiane Martins Volcao, Marina dos Santos, Flavio Manoel Rodrigues da Silva Junior
Summary: Socioeconomic and demographic factors, lifestyle, and cultural characteristics may have an important impact on genetic damage. This study analyzed the association between a set of factors and genetic damage by studying populations in southern Brazil. The results showed that age had a weak association with genetic damage, while factors such as skin color, occupational exposure, drug use, exposure to radiation, and the cultural habit of consuming chimarrao were found to be associated with genetic damage. Yerba mate consumption was identified as a confounding factor in cytogenetic studies in the southern region of South America.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Food Science & Technology
Antonio Cascajosa-Lira, Maria Puerto, Ana I. Prieto, Silvia Pichardo, Leticia Diez-Quijada Jimenez, Alberto Banos, Enrique Guillamon, Rosario Moyano, Veronica Molina-Hernandez, Angeles Jos, Ana M. Camean
Summary: This study confirmed the lack of in vivo genotoxicity of PTS at the doses tested, indicating that it is a promising natural alternative in animal feed without adverse effects on genetic material.
Article
Food Science & Technology
Concepcion Medrano-Padial, Maria Puerto, Ana Isabel Prieto, Nahum Ayala, Pauline Beaumont, Caroline Rouger, Stephanie Krisa, Silvia Pichardo
Summary: This study investigated the in vivo genotoxic effects of rats orally exposed to different doses of ST-99 and found no genotoxicity in the cells isolated from stomach, liver, and blood. Analytical studies confirmed the presence of stilbenes and their metabolites in plasma and tissues, supporting the safety profile of ST-99 for potential application as a preservative in the wine industry.
Article
Biochemistry & Molecular Biology
Kamil Brzoska, Malgorzata Szczygiel, Agnieszka Drzal, Martyna Sniegocka, Dominika Michalczyk-Wetula, Eva Biela, Martyna Elas, Lucyna Kapka-Skrzypczak, Hanna Lewandowska-Siwkiewicz, Krystyna Urbanska, Marcin Kruszewski
Summary: Gold nanoparticles (AuNPs) were investigated for their potential anticancer effects in tumor growth and metastasis. The results showed that AuNPs did not inhibit tumor growth but caused significant vasodilation in the tumor tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Krzysztof Sawicki, Magdalena Czajka, Magdalena Matysiak-Kucharek, Jacek Kurzepa, Paulina Wojtyla-Buciora, Karol Zygo, Marcin Kruszewski, Lucyna Kapka-Skrzypczak
Summary: This study investigated the impact of the pesticide chlorpyrifos (CPS) on the expression of enzymes involved in synthesis and metabolism of vitamin D3 in human keratinocytes and fibroblasts. The results indicate that exposure to CPS significantly altered the expression of cytochrome P450 isoforms in cells, potentially disrupting vitamin D3 metabolism. Further research is needed to better understand these mechanisms.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Oncology
Monika Ruzycka-Ayoush, Patrycja Kowalik, Agata Kowalczyk, Piotr Bujak, Anna M. Nowicka, Maria Wojewodzka, Marcin Kruszewski, Ireneusz P. Grudzinski
Summary: A new targeted drug delivery system based on Ag-In-Zn-S quantum dots was developed for lung cancer treatment. The system demonstrated significant cytotoxicity and genotoxicity effects, showing potential as an effective therapy for lung cancer.
CANCER NANOTECHNOLOGY
(2021)
Article
Medicine, Research & Experimental
Aleksandra Paterek, Barbara Sochanowicz, Marta Okninska, Witold Smigielski, Marcin Kruszewski, Urszula Mackiewicz, Michal Maczewski, Przemyslaw Leszek
Summary: Ivabradine may prevent the detrimental effects of dopamine treatment during exacerbations of chronic heart failure, potentially offering additional benefits beyond heart rate reduction. However, preventing ventricular HCN4 overexpression may not play a major role in this setting.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Genetics & Heredity
Somayeh Shahmoradi Ghahe, Konrad Kosicki, Maria Wojewodzka, Bartosz A. Majchrzak, Anna Fogtman, Roksana Iwanicka-Nowicka, Agata Ciuba, Marta Koblowska, Marcin Kruszewski, Barbara Tudek, Elzbieta Speina
Summary: Resistance to photodynamic therapy in cancer cells may be caused by various mechanisms, including efficient repair of oxidative DNA damage and DNA breaks. Higher activity of APE1 endonuclease and increased expression and activation of DNA damage kinase ATM in the U-87 MGR cell line suggest that they are potential targets for sensitizing resistant cells to PDT.
Article
Biochemistry & Molecular Biology
Anna Lankoff, Malwina Czerwinska, Rafal Walczak, Urszula Karczmarczyk, Kamil Tomczyk, Kamil Brzoska, Giulio Fracasso, Piotr Garnuszek, Renata Mikolajczak, Marcin Kruszewski
Summary: Metastatic castration-resistant prostate cancer is a progressive and incurable disease with poor prognosis. PSMA-based targeted radioimmunotherapy shows promise in extending patients' lives, but there are still safety concerns that need to be addressed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Damian Krzyzanowski, Marcin Kruszewski, Agnieszka Grzelak
Summary: Silver nanoparticles (AgNPs) have unique properties that have attracted significant attention, showing antibacterial, antifungal, and anticancer properties. However, they can also exhibit cytotoxic effects and modulate the activity and expression of ABC transporters.
Article
Biochemistry & Molecular Biology
Katarzyna Dziendzikowska, Jacek Wilczak, Wojciech Grodzicki, Joanna Gromadzka-Ostrowska, Malgorzata Wesierska, Marcin Kruszewski
Summary: The study found that silver nanoparticles disrupted the antioxidant system in the hippocampus of rats and induced oxidative stress in a coating-dependent manner, which could potentially lead to neurodegeneration and cognitive disorders. The coating material of the nanoparticles also modulated the concentrations of steroids and the expression of genes involved in steroid synthesis and metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Bogna Kozlowska, Barbara Sochanowicz, Leszek Kraj, Malgorzata Palusinska, Piotr Kolsut, Lukasz Szymanski, Slawomir Lewicki, Witold Smigielski, Marcin Kruszewski, Przemyslaw Leszek
Summary: This study investigated the changes in iron-related proteins in the myocardium of heart failure patients. The results showed reduced expression of several proteins responsible for iron transport and storage, along with increased expression of oxidative stress markers. The reduction of iron-gathering proteins and elevated oxidative stress in failing hearts is detrimental to myocardial cells.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Chemistry, Physical
Kamil Brzoska, Maria Wojewodzka, Malgorzata Szczygiel, Agnieszka Drzal, Martyna Sniegocka, Dominika Michalczyk-Wetula, Eva Biela, Martyna Elas, Malgorzata Kucinska, Hanna Piotrowska-Kempisty, Lucyna Kapka-Skrzypczak, Marek Murias, Krystyna Urbanska, Marcin Kruszewski
Summary: This study analyzed the impact of different silver nanoparticles on the development and metastatic potential of breast cancer using a mouse model. The results showed that citrate-coated silver nanoparticles had a significant effect on reducing tumor volume and lung metastases in mice treated intragastrically. However, no effect on lung metastases was observed after intravenous administration of silver nanoparticles. The findings suggest that the anticancer activity of silver nanoparticles may depend on the activation of the immune system.
Article
Biochemistry & Molecular Biology
Michal Oczkowski, Jacek Wilczak, Katarzyna Dziendzikowska, Johan Ovrevik, Oddvar Myhre, Anna Lankoff, Marcin Kruszewski, Joanna Gromadzka-Ostrowska
Summary: This study investigated the effects of exposure to biodiesel exhaust emissions on oxidative stress in testes using an animal model. The results showed that dietary intervention with blackcurrant pomace could reduce oxidative stress in testes to some extent.
Review
Biology
Bogna Kozlowska, Barbara Sochanowicz, Leszek Kraj, Malgorzata Palusinska, Piotr Kolsut, Lukasz Szymanski, Slawomir Lewicki, Marcin Kruszewski, Marta Zaleska-Kociecka, Przemyslaw Leszek
Summary: Heart failure (HF) is a common disease and iron deficiency (ID) plays a significant role in worsening the condition of HF patients. Iron supplementation is recommended but may have adverse effects. There are still many knowledge gaps regarding the interaction between iron deficiency and HF. Summarizing the latest translational and clinical research on iron deficiency in HF is important for raising awareness among clinicians.
Review
Biochemistry & Molecular Biology
Anna Lankoff, Malwina Czerwinska, Marcin Kruszewski
Summary: Prostate cancer is a common and deadly malignancy in men, with limited treatment options for advanced stages. Nanoparticles have been developed to selectively target cancer cells and improve imaging and therapy. This review focuses on the selection criteria and progress of nanoparticle-based radioconjugates for targeted imaging and therapy of prostate cancer.
Article
Biochemistry & Molecular Biology
Magdalena Czajka, Krzysztof Sawicki, Magdalena Matysiak-Kucharek, Marcin Kruszewski, Jacek Kurzepa, Paulina Wojtyla-Buciora, Lucyna Kapka-Skrzypczak
Summary: Chlorpyrifos, a representative of organophosphorus pesticides, has effects on the viability, proliferation, differentiation, and fatty acid uptake in 3T3-L1 cells. Exposure to chlorpyrifos decreases cell viability but increases it at low concentrations. It inhibits proliferation and differentiation of preadipocytes, reduces lipid accumulation, and down-regulates key transcription factors. In fully differentiated adipocytes, chlorpyrifos increases basal free fatty acid uptake. These findings suggest that chlorpyrifos may play a role in the development of obesity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Physical
Katarzyna Sikorska, Krzysztof Sawicki, Magdalena Czajka, Lucyna Kapka-Skrzypczak, Marcin Kruszewski, Kamil Brzoska
Summary: The interest in nanoparticles and their effects on living organisms has been increasing in recent decades. Non-metallic nanoparticles appear to be less toxic than metallic ones, leading to their growing use in medicine and industry. This review summarizes current knowledge of the in vitro and in vivo neurotoxicity of non-metallic nanoparticles, as well as the mechanisms associated with their positive or negative effects on the central nervous system (CNS).
