期刊
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
卷 15, 期 3, 页码 2727-2738出版社
ESG
DOI: 10.20964/2020.03.62
关键词
HIV gene; Differential pulse voltammetry; Amino-reduced graphene oxide; Biosensor; beta-Cyclodextrin
资金
- National Natural Science Foundation of China [21864006, 21563006, 21763005]
- Natural Science Foundation of Guangxi Province [2017GXNSFDA198034, 2017GXNSFFA198005]
- Guangxi Scientific and Technological Development Projects [AD17195081]
- Guangxi Higher Education Institutes [guijiaoren[2017]38]
- BAGUI Scholar Program of Guangxi Province of China
A differential pulse voltammetry (DPV) platform for the sensing of human immunodeficiency virus (HIV) gene was described through the self-assembling of amino-reduced graphene oxide (NH2-rGO) and beta-cyclodextrin (beta-CD) on the surface of glassy carbon electrode (GCE). It is making use of the single-stranded DNA (ssDNA) with sequence hybridized with HIV gene. The ssDNA was attached on NH2-rGO/beta-CD modified GCE (NH2-rGO/beta-CD/GCE) surface, then methylene blue (MB) was interacted with ssDNA to general obvious DPV signal. The HIV gene was then initiated to hybridize with ssDNA on NH2-rGO/beta-CD/GCE surface, causing a dramatic decrease in DPV response of MB at potential of -0.25 V (vs. Ag/AgCl). Under optimal experimental conditions, response was linear in the 0.05 pM to 1 pM HIV gene concentration range. The electrochemical biosensor displayed an ultrahigh sensitivity with detection limit of 8.7 fM and excellent selectivity. The modified electrode displayed good reproducibility, ultra-high sensitivity, and terrific selectivity. This electrochemical biosensor was successfully used for the determination of HIV gene in human serum samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据