期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 153, 期 -, 页码 931-941出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.02.216
关键词
Reduced graphene oxide; Cationic poly(allylamine hydrochloride); Folic acid; Near infrared; Deoxyribonucleic acid (DNA) release
资金
- Science and Engineering Research Board (SERB), Department of Science and Technology, Government of India [SERB/F/5627/2015-16]
- DST-INSPIRE [IF160513]
- BioX Centre, Indian Institute of Technology Mandi
- AdvancedMaterials Research Centre (AMRC), Indian Institute of Technology Mandi
Reduced graphene oxide (RGO) has shown tremendous potential as a MR responsive nanomaterial and has been extensively explored for NIR mediated photothermal therapy and drug delivery. However, the potential of NIR as a stimulus to trigger release of entrapped/complexed DNA from its surface have not been explored. Strong complexation between the loaded cargo and the carrier often leads to no-release or decrease in the release of the therapeutic cargo. Herein, we investigated NIR as a stimulus for inducing DNA release from RGO nanocomposites. A quaternary ammonium modified poly(allylamine hydrochloride) functionalized RGO nanocomposite (RGO-MPAH) was synthesized, which was further tagged with a targeting moiety, folic add (FA).The structural, optical and chemical properties of the synthesized nanocomposites were characterized which validated successful reduction and functionalization of GO with PAH/MPAH. The nanocomposites were found to be non-toxic and showed excellent DNA binding ability at complexation ratios as low as 3:1 (w/w). Additionally, the nanocomposites demonstrated NIR responsive release of complexed DNA from their surfaces, with RGO-PAH showing maximum DNA release followed by RGO-MPAH and RGO-MPAH-FA. This study shows the potential of NIR light to act as a stimulus for inducing release of entrapped nucleic acids from the surface of nanocarriers. (C) 2020 Elsevier B.V. All rights reserved.
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