期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 145, 期 -, 页码 740-749出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2019.12.218
关键词
Russula alutacea Fr.; Inflammation; Zebrafish (Danio rerio) larvae
资金
- Science and Technology Department of Zhejiang Province [2018C02045]
Russula alutacea Fr. (RaF) is a tasty mushroom with high nutritional value and have been regarded as food by local people for a long time. However, few researches have focused on the polysaccharide in RaF. In this study, a purified polysaccharide (Rap-1) was isolated with an average molecular weight of 1029.7 kDa. In vitro, Rap-1 significantly suppressed cell morphological changes and inhibited the production of nitric oxide (NO) in LPS-induced RAW 264.7 cells extracellularly and intracellularly. Besides, Rap-1 also down-regulated the expression of nuclear factor kappa-B (NF-kappa B), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) contents. Rap-1 ameliorated the oxidative stress and mitochondrial dysfunction caused by LPS via MAPKs signaling pathways in macrophages. In vivo, Rap-1 decreased the ROS and O-2(-) levels and recovered the heart in zebrafish (Danio rerio) larvae induced by LPS, These results together suggested that Rap-1 could be a potential functional resource to protect against inflammatory and oxidative damage. (C) 2019 Elsevier B.V. All rights reserved.
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