Review
Pharmacology & Pharmacy
Ruth Nussinov, Chung-Jung Tsai, Hyunbum Jang
Summary: Driver mutations play a critical role in cancer initiation and progression, with drug resistance being a common challenge in pharmacological treatment. The emergence of drug resistance can be attributed to a variety of mechanisms, such as mutations occurring in the same or different proteins, and in the same or parallel pathways. The complexity of genetic alterations and tumor microenvironment changes poses challenges in predicting drug resistance and requires further research in precision medicine.
DRUG RESISTANCE UPDATES
(2021)
Article
Medicine, Research & Experimental
Onat Kadioglu, Mohamed E. M. Saeed, Nuha Mahmoud, Shaymaa Azawi, Kristin Mrasek, Thomas Liehr, Thomas Efferth
Summary: The study characterized U87.MG Delta EGFR glioblastoma cells with constitutively active EGFR and identified novel drug resistance mechanisms related to the expression of mutation-activated EGFR. The cells showed numerous chromosomal aberrations and gene overexpression, including well-known drug resistance genes and novel ones. These findings may have important implications for future treatment strategies.
Article
Biology
Antonella Raffo-Romero, Soulaimane Aboulouard, Emmanuel Bouchaert, Agata Rybicka, Dominique Tierny, Nawale Hajjaji, Isabelle Fournier, Michel Salzet, Marie Duhamel
Summary: Developing a robust canine mammary tumor model in the form of tumoroids has been achieved, which can replicate the diversity and heterogeneity of tumors. Canine tumoroids are a reliable drug screening model and can be cryopreserved to create a biobank.
Article
Chemistry, Multidisciplinary
Xiaoyan Liu, Wei Zhang, Wenfu Zheng, Xingyu Jiang
Summary: The study highlights the vital role of the neural microenvironment in cancer development and metastasis, emphasizing the need for drug candidates targeting this microenvironment. The mu CHC system integrates a micro-culture chip with high-content analysis for efficient drug screening and interaction studies between neurons and cancer cells.
Article
Chemistry, Multidisciplinary
Wanheng Zhang, Yan Chen, Mengyuan Li, Shucheng Cao, Nana Wang, Yingjian Zhang, Yongtao Wang
Summary: 2D cell culture plays a crucial role in cancer research and drug discovery, but it inadequately models the biology of tumors in vivo. 3D tumor culture systems provide a better mimicry of tumor characteristics and pose significant challenges. In this study, polydopamine-modified decellularized lung scaffolds are designed to study tumor progression, drug screening, and mimic the tumor microenvironment. The modified scaffolds with enhanced hydrophilicity and cell compatibility promote cell growth and proliferation. The observed higher survival rates in the modified scaffolds after treatment with anticancer drugs suggest their potential application in drug resistance and immunotherapy screening.
Article
Biochemistry & Molecular Biology
Unnati Modi, Dhaval Kedaria, Rajesh Vasita
Summary: The chemically modified Dextran-Chitosan (MDC) hydrogel has been developed as a 3D tumoroid aiding scaffold to create a physiologically relevant in vitro model of lung adenocarcinoma. This innovative model allows for the study of disease mechanisms and drug efficacy on relevant 3D cancer models in vitro.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Medicine, Research & Experimental
Wei-Zhi Lin, Yen-Chun Liu, Meng-Chang Lee, Chi-Tun Tang, Gwo-Jang Wu, Yu-Tien Chang, Chi-Ming Chu, Chia-Yang Shiau
Summary: This study identified 12 candidate drugs for the potential treatment of glioblastoma based on genome-wide association studies. The results showed that these candidate drugs were more effective in inhibiting glioma cell lines compared to the standard treatment.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Review
Pharmacology & Pharmacy
Ge Gao, Minjun Ahn, Won-Woo Cho, Byoung-Soo Kim, Dong-Woo Cho
Summary: Advances in 3D printing technology and tailored biomaterials have enabled precise fabrication of biological components and complex 3D geometrics, as well as the development of customized drug screening and drug delivery systems in pharmaceutical applications. This review discusses the necessary elements for advanced pharmaceutical techniques, state-of-the-art 3D printing technology, and limitations of traditional approaches in drug screening and drug delivery systems.
Article
Oncology
Vladimir Shapovalov, Liliya Kopanitsa, Lavinia-Lorena Pruteanu, Graham Ladds, David S. Bailey
Summary: Glioblastoma remains a challenging cancer with limited treatment options, and future therapies will require personalized approaches targeting aggressive cellular phenotypes. The use of DIGEX has identified genes encoding drug targets and validated effective antiproliferative agents in human GBM cell models. Further research using this method may uncover new therapeutic opportunities and diagnostic strategies for the complex drug response networks in GBM.
Article
Biochemistry & Molecular Biology
Beata Pajak, Ewelina Siwiak-Niedbalska, Anna Jaskiewicz, Maja Soltyka, Tomasz Domoradzki
Summary: Glioblastoma multiforme (GBM) is a common primary malignant brain tumor with limited treatment options due to the blood-brain barrier (BBB). This study focuses on the design and synthesis of small-molecule anticancer agents that can penetrate the BBB, with a specific focus on the compound 2-deoxy-D-glucose (2-DG). A derivative of 2-DG called WP1234 has shown promising anticancer potential in vitro, inhibiting GBM cell viability, glycolysis, and histone deacetylases (HDAC) activity. Further studies are needed to evaluate its efficacy and safety in animal models and toxicology studies.
Article
Biochemistry & Molecular Biology
Sakthi Lenin, Elise Ponthier, Kaitlin G. Scheer, Erica C. F. Yeo, Melinda N. Tea, Lisa M. Ebert, Mariana Oksdath Mansilla, Santosh Poonnoose, Ulrich Baumgartner, Bryan W. Day, Rebecca J. Ormsby, Stuart M. Pitson, Guillermo A. Gomez
Summary: This study conducted a systematic review on the molecular mechanisms driving glioblastoma progression, identifying 65 drugs/inhibitors for screening their efficacy on patient-derived glioma stem cells. Costunolide, a TERT inhibitor, was found effective in reducing cell viability in both primary and pre-treated tumor models. This novel workflow could lead to personalized and effective treatment for recurrent glioblastoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemical Research Methods
Mike Fang, Brian Richardson, Cheryl M. Cameron, Jean-Eudes Dazard, Mark J. Cameron
Summary: dpGSEA is a modified GSEA method that can detect phenotypically relevant drug targets through transcriptomic enrichment. It has been shown to effectively detect perturbation of specific drugs in independent datasets, and identify drug targeting molecules in differentially expressed genes in immune cells from HIV-infected individuals.
BMC BIOINFORMATICS
(2021)
Article
Biochemistry & Molecular Biology
Beata Pajak, Ewelina Siwiak-Niedbalska, Anna Jaskiewicz, Maja Soltyka, Rafal Zielinski, Tomasz Domoradzki, Izabela Fokt, Stanislaw Skora, Waldemar Priebe
Summary: Research on 2-DG and its analogs has made significant progress in the past decade. Challenges in using 2-DG as a monotherapy have led researchers to focus on enhancing its bioavailability and exploring combinations with glycolysis inhibitors to synergistically eliminate cancer cells. The study presents promising evidence of synergistic cytotoxic effects in glioblastoma cells using 2-DG analogs combined with HDAC inhibitors.
Article
Biochemistry & Molecular Biology
Jihye Shin, Yinhua Piao, Dongmin Bang, Sun Kim, Kyuri Jo
Summary: Recent studies on drug sensitivity prediction have utilized graph neural networks and model interpretability, but it is important to develop a prediction model that is both knowledge-guided and interpretable. In this study, we propose the DRPreter model for predicting the response of anticancer drugs, using graph neural networks and domain knowledge on biological pathways to interpret the mechanism of drug action.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Hui Niu, Jiarui Xiao, Xiaoli Lou, Lingling Guo, Yongsheng Zhang, Runhuai Yang, Hao Yang, Shouli Wang, Fuzhou Niu
Summary: This study utilized natural silk fibroin and chitosan to fabricate 3D microscaffolds with good biocompatibility for cancer cell culture and drug sensitivity testing. The scaffolds were characterized using various testing methods and showed promising results in facilitating cell growth and drug sensitivity screening.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Nudjanad Heebkaew, Narawadee Rujanapun, Phongsakorn Kunhorm, Thiranut Jaroonwitchawan, Nipha Chaicharoenaudomrung, Wilasinee Promjantuek, Parinya Noisa
BIOMED RESEARCH INTERNATIONAL
(2019)
Article
Cell Biology
Nipha Chaicharoenaudomrung, Phongsakorn Kunhorm, Wilasinee Promjantuek, Nudjanad Heebkaew, Narawadee Rujanapun, Parinya Noisa
JOURNAL OF CELLULAR PHYSIOLOGY
(2019)
Article
Food Science & Technology
Ali Hamzeh, Parinya Noisa, Jirawat Yongsawatdigul
JOURNAL OF FUNCTIONAL FOODS
(2020)
Article
Multidisciplinary Sciences
Siriwalee Siriwibool, Nantawat Kaekratoke, Kantapat Chansaenpak, Kittipan Siwawannapong, Pannipa Panajapo, Kritsana Sagarik, Parinya Noisa, Rung-Yi Lai, Anyanee Kamkaew
SCIENTIFIC REPORTS
(2020)
Article
Food Science & Technology
Xiaogang Zhang, Parinya Noisa, Jirawat Yongsawatdigul
Article
Oncology
Palakorn Kaokaen, Atchara Jaiboonma, Nipha Chaicharoenaudomrung, Phongsakorn Kunhorm, Kajohnkiart Janebodin, Parinya Noisa, Paiboon Jitprasertwong
Summary: This study found that cordycepin-loaded cassava starch nanoparticles can enhance the proliferation, protein secretion, and expression of salivary-specific genes in submandibular gland cells, while also protecting and scavenging ROS in HSGs and inhibiting the growth of human oral squamous carcinoma cells. The encapsulation of CS with cassava nanoparticles enhances the activity of CS, presenting dual actions against HSGs and HSC-4 cells.
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Thitima Pewklang, Sirawit Wet-osot, Sirilak Wangngae, Utumporn Ngivprom, Kantapat Chansaenpak, Chuthamat Duangkamol, Rung-Yi Lai, Parinya Noisa, Mongkol Sukwattanasinitt, Anyanee Kamkaew
Summary: The hypoxia-responsive probe AZO-Flav, based on a flavylium dye containing an azo group, was synthesized for rapid detection of hypoxic conditions in cancer cells. It displayed high enzyme specificity and sensitivity, making it a promising tool for in vivo hypoxia detection applications.
Article
Food Science & Technology
Xiaogang Zhang, Parinya Noisa, Jirawat Yongsawatdigul
Summary: Nine novel peptides were isolated from tilapia hydrolysate, with LPGYF and SC showing the best ABTS.+ scavenging capacity. Peptides containing C and Y were more effective than ascorbic acid, while K-containing peptides exhibited lower antioxidant activity. These peptides demonstrated potent intracellular reactive oxygen species scavenging capabilities, and were able to upregulate the expression of antioxidant enzymes in cells, with PGY being identified as the most effective cellular antioxidant.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Biotechnology & Applied Microbiology
Phongsakorn Kunhorm, Phongsakorn Chueaphromsri, Nipha Chaicharoenaudomrung, Parinya Noisa
Summary: In this study, the epigenetic status of C. militaris was modified using valproic acid (VPA), resulting in a significant enhancement of cordycepin production. By upregulating key genes, VPA successfully increased the production of cordycepin, suggesting that this strategy could be applicable to other microorganism cultures.
BIOTECHNOLOGY LETTERS
(2022)
Article
Cell & Tissue Engineering
Palakorn Kaokaen, Natchadaporn Sorraksa, Ruchee Phonchai, Nipha Chaicharoenaudomrung, Phongsakorn Kunhorm, Parinya Noisa
Summary: The study found that nanoencapsulated cordyceps and turmeric extracts can accelerate neuronal maturation and alleviate neuronal pathology in human neural cells. This opens up new possibilities for nanotechnology-driven drug delivery systems in the future.
CELLULAR AND MOLECULAR BIOENGINEERING
(2023)
Article
Nanoscience & Nanotechnology
Prapassara Muangsopa, Kantapat Chansaenpak, Sastiya Kampaengsri, Jadsada Saetiew, Parinya Noisa, Panomsak Meemon, Anyanee Kamkaew
Summary: This study developed a carrier-free nanosystem for imaging and photodynamic/chemo combination therapy. The resulting nanoparticles showed a synergistic anticancer effect and high PDT efficiency in breast cancer cells. Optical coherence tomography was used as a monitoring tool. These findings suggest a great potential use for I2-IR783/MTX@NPs in breast cancer theranostic application.
ACS APPLIED BIO MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Nudjanad Heebkaew, Wilasinee Promjantuek, Nipha Chaicharoenaudomrung, Ruchee Phonchai, Phongsakorn Kunhorm, Natchadaporn Soraksa, Parinya Noisa
Summary: In this study, the HaCaT secretome was combined with polyesters and cassava starch, which enhanced cell proliferation, migration, and anti-aging effects while reducing reactive oxygen species levels. This suggests that the HaCaT secretome may have potential applications in cosmetics for wound prevention and healing.
MOLECULAR BIOTECHNOLOGY
(2023)
Article
Chemistry, Medicinal
Amorn Pangjantuk, Phongsakorn Chueaphromsri, Phongsakorn Kunhorm, Ruchee Phonchai, Sirilak Chumkiew, Nipha Chaicharoenaudomrung, Parinya Noisa
Summary: This study found that hispolon can inhibit the proliferation of breast cancer cells and induce their apoptosis. It also regulates the expression of apoptosis-related genes, increases intracellular reactive oxygen species levels, downregulates antioxidant gene expression, and activates autophagy as a potential therapeutic mechanism.
JOURNAL OF BIOLOGICALLY ACTIVE PRODUCTS FROM NATURE
(2023)
Article
Medicine, General & Internal
Atchara Jaiboonma, Palakorn Kaokaen, Nipha Chaicharoenaudomrung, Phongsakorn Kunhorm, Kajohnkiart Janebodin, Parinya Noisa, Paiboon Jitprasertwong
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
(2020)