期刊
BIORESOURCE TECHNOLOGY
卷 180, 期 -, 页码 47-53出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.12.072
关键词
Recombinant lipase; Microalgae oil; Esterification reaction; Biodiesel; Conversion efficiency
资金
- Chinese High Technology Research and Development Program [2013AA065802]
- Chinese Scientific and Technical Supporting Programs [2013BAD10B01]
- Undergraduate Innovation Program of China Agricultural University [J1103520]
A recombinant Rhizomucor miehei lipase was constructed and expressed in Pichia pastoris. The target enzyme was termed Lipase GH2 and it can be used as a free enzyme for catalytic conversion of microalgae oil mixed with methanol or ethanol for biodiesel production in an n-hexane solvent system. Conversion rates of two major types of biodiesel, fatty acid methyl ester (FAME) and fatty acid ethyl ester (FAEE), reached maximal values (>90%) after 24 h. The process of FAME production is generally more simple and economical than that of FAEE production, even though the two processes show similar conversion rates. In spite of the damaging effect of ethanol on enzyme activity, we successfully obtained ethyl ester by the enzymatic method. Our findings indicate that Lipase GH2 is a useful catalyst for conversion of microalgae oil to FAME or FAEE, and this system provides efficiency and reduced costs in biodiesel production. (C) 2014 Elsevier Ltd. All rights reserved.
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