Article
Biochemistry & Molecular Biology
Bernhard Luscher, Ivan Ahel, Matthias Altmeyer, Alan Ashworth, Peter Bai, Paul Chang, Michael Cohen, Daniela Corda, Francoise Dantzer, Matthew D. Daugherty, Ted M. Dawson, Valina L. Dawson, Sebastian Deindl, Anthony R. Fehr, Karla L. H. Feijs, Dmitri V. Filippov, Jean-Philippe Gagne, Giovanna Grimaldi, Sebastian Guettler, Nicolas C. Hoch, Michael O. Hottiger, Patricia Korn, W. Lee Kraus, Andreas Ladurner, Lari Lehtio, Anthony K. L. Leung, Christopher J. Lord, Aswin Mangerich, Ivan Matic, Jason Matthews, George-Lucian Moldovan, Joel Moss, Gioacchino Natoli, Michael L. Nielsen, Mario Niepel, Friedrich Nolte, John Pascal, Bryce M. Paschal, Krzysztof Pawlowski, Guy G. Poirier, Susan Smith, Gyula Timinszky, Zhao-Qi Wang, Jose Yelamos, Xiaochun Yu, Roko Zaja, Mathias Ziegler
Summary: ADP-ribosylation, a post-translational modification of proteins, nucleic acids, and metabolites, plays diverse roles in cellular processes such as stress responses, signaling, and transcriptional regulation. Recent advances in research have identified a wide range of cellular pathways regulated by ADP-ribosylation, highlighting the importance of understanding this mechanism in cell biology.
Article
Biochemistry & Molecular Biology
Mirko M. Maksimainen, Sudarshan Murthy, Sven T. Sowa, Albert Galera-Prat, Elena Rolina, Juha P. Heiskanen, Lari Lehtio
Summary: By modifying the structure of inhibitor molecules, the selectivity was successfully shifted from poly-ADP-ribosyltransferases to mono-ADP-ribosyltransferases, and the binding modes of analogs were experimentally verified. The study demonstrates a method to differentiate compound selectivity between mono- and poly-ribosyltransferases in the human ARTD family.
BIOORGANIC & MEDICINAL CHEMISTRY
(2021)
Review
Immunology
Huifang Zhu, Yan-Dong Tang, Guoqing Zhan, Chenhe Su, Chunfu Zheng
Summary: PARPs, a family of proteins responsible for transferring ADP-ribose groups, play important roles in regulating various cellular functions and have widespread involvement in mammalian innate immunity. Understanding the roles of PARPs and ADP-ribosylation in regulating innate immune responses can help in discovering and designing new molecular targets for cancers, pathogenic infections, and inflammations.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marcin J. Suskiewicz, Luca Palazzo, Rebecca Hughes, Ivan Ahel
Summary: Despite years of research on ADP-ribosyltransferases from the PARP family, their substrate specificity has remained unclear. Recent breakthroughs include identifying protein serine residues, cysteine, and tyrosine as potential targets of specific PARPs. These findings shed new light on PARP-mediated catalysis and caution to expect the unexpected with potential substrates.
Review
Microbiology
Katharina Biaesch, Sarah Knapp, Patricia Korn
Summary: Cells have developed various strategies to defend against viral infections, one of which is distinguishing foreign molecules from their own. This is achieved through the perception of foreign nucleic acids by host proteins, leading to an effective immune response. Pattern recognition receptors and RNA-binding proteins play crucial roles in sensing and distinguishing viral RNA from host RNA. Recent findings suggest that some ADP-ribosyltransferases (ARTs; PARP9-PARP15) contribute to immune defense and viral attenuation, but their activation, targets, and interference mechanisms are not well understood. One well-known RNA sensor, PARP13, has antiviral activities, and PARP9 has recently been described as a sensor for viral RNA.
Review
Biochemistry & Molecular Biology
Karla L. H. Feijs, Roko Zaja
Summary: Post-translational modifications play a crucial role in regulating cell health. The PARP family enzymes can transfer ADP-ribose to their targets, and recent studies have expanded the list of potential acceptor amino acids. While PARylation by PARP1 is well studied, knowledge about other family members such as PARP7 and PARP10 is limited. Understanding the biochemical reactions and biological functions of PARPs is important for future research.
BIOSCIENCE REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Kelsie M. Rodriguez, Michael S. Cohen
Summary: In this review, the design principles behind two chemical genetic strategies for identifying direct protein substrates of individual PARP family members are discussed, providing novel insights into the cellular function of individual PARPs and PARP-mediated ADP-ribosylation.
TRENDS IN BIOCHEMICAL SCIENCES
(2022)
Review
Cell Biology
Kira Schuetzenhofer, Johannes Gregor Matthias Rack, Ivan Ahel
Summary: ADP-ribosylation is a common posttranslational modification involved in DNA repair, where serine has been identified as the primary acceptor of ADP-ribosyl moiety following DNA damage. This signal is dependent on HPF1 and ARH3 for degradation and cleavage of the initial poly(ADP-ribose) chain.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Evgeniia Prokhorova, Florian Zobel, Rebecca Smith, Siham Zentout, Ian Gibbs-Seymour, Kira Schutzenhofer, Alessandra Peters, Josephine Groslambert, Valentina Zorzini, Thomas Agnew, John Brognard, Michael L. Nielsen, Dragana Ahel, Sebastien Huet, Marcin J. Suskiewicz, Ivan Ahel
Summary: Recent findings suggest that serine ADP-ribosylation plays a vital role in cellular responses to PARP1/PARP2 inhibitors. The efficient modification of three serine residues within PARP1 by HPF1 counters PARP1 trapping and contributes to inhibitor tolerance, implicating these residues as potential biomarkers for PARP inhibitor therapy.
NATURE COMMUNICATIONS
(2021)
Review
Microbiology
Diego V. Santinelli-Pestana, Elena Aikawa, Sasha A. Singh, Masanori Aikawa
Summary: Aberrant adenosine diphosphate-ribose (ADP)-ribosylation is linked to various diseases. In this review, the roles of PARP1/ARTD1, PARP7/ARTD14, PARP9/ARTD9, and PARP14/ARTD8 in macrophages are discussed. PARPs/ARTs regulate macrophage changes in chronic inflammation through catalytic and non-catalytic mechanisms. Understanding these complex mechanisms and developing innovative technologies are crucial for the development of new therapeutics.
Review
Medicine, Research & Experimental
Jing-Jing Zha, Yi Tang, Ya-Lan Wang
Summary: Current knowledge on ADP-ribosylation modifications of histones, especially mono-ADP-ribosylation modifications, is limited. Recent studies have identified more mono-ADP-ribosyltransferases, making mono-ADP-ribosylation a popular research topic. Mono-ADP-ribosylated histones are involved in various physiological or pathological processes, with limited research methods compared to poly-ADP-ribosylation histone modification.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Multidisciplinary Sciences
Katherine E. Zarn, Sierra A. Jaramillo, Anthony R. Zapata, Nathan E. Stone, Ashley N. Jones, Haley E. Nunnally, Erik W. Settles, Ken Ng, Paul S. Keim, Steen Knudsen, Patricia M. Nuijten, Aloys S. L. Tijsma, Christopher T. French
Summary: A recent study found that the poly (ADP-ribose) polymerase (PARP) inhibitor stenoparib effectively inhibits various strains of SARS-CoV-2 in vitro, including the alpha variant, with the combination of remdesivir resulting in a synergistic effect.
Article
Biochemistry & Molecular Biology
Andrea Hlousek-Kasun, Petra Mikolcevic, Johannes Gregor Matthias Rack, Callum Tromans-Coia, Marion Schuller, Gytis Jankevicius, Marija Matkovic, Branimir Bertosa, Ivan Ahel, Andreja Mikoc
Summary: ADP-ribosylation is an important covalent modification that plays critical roles in various biological processes. The toxin/antitoxin pair DarT/DarG has been discovered, where DarT modifies DNA and DarG reverses this modification. The homologue SCO6735 is highly conserved and associated with antibiotic production in bacteria, and it can also reverse the ADP-ribosylation catalyzed by DarT.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Maike Lehner, Sonja Rieth, Eva Hoellmueller, Daniel Spliesgar, Bastian Mertes, Florian Stengel, Andreas Marx
Summary: This study presents two new NAD(+) derivatives, combined with methods for mild cell delivery, allowing for the study of ADP-ribosylation in living human cells. The approach enables imaging of ADP-ribosylation and proteome-wide analysis of cellular adaptation through protein ADP-ribosylation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Cell Biology
Ping Li, Yushuang Lei, Jia Qi, Wanqin Liu, Kai Yao
Summary: ADP-ribosylation, a reversible post-translational modification, is tightly regulated by its writers, readers and erasers. It plays critical roles in various physiological and pathological processes. Understanding the major players in the ADP-ribosylation cycle can contribute to research on its function and treatment of associated diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
