Integrating gene delivery and gene-editing technologies by adenoviral vector transfer of optimized CRISPR-Cas9 components

The Chromatin Structure of CRISPR-Cas9 Target DNA Controls the Balance between Mutagenic and Homology-Directed Gene-Editing Events

(2019) Josephine M. Janssen et al.   Molecular Therapy-Nucleic Acids

Optimized base editors enable efficient editing in cells, organoids and mice

(2018) Maria Paz Zafra et al.   NATURE BIOTECHNOLOGY

A highly specific SpCas9 variant is identified by in vivo screening in yeast

(2018) Antonio Casini et al.   NATURE BIOTECHNOLOGY

Engineering the Delivery System for CRISPR-Based Genome Editing

(2018) Zachary Glass et al.   TRENDS IN BIOTECHNOLOGY

Comparison of Various Nuclear Localization Signal-Fused Cas9 Proteins and Cas9 mRNA for Genome Editing in Zebrafish

(2018) Peinan Hu et al.   G3-Genes Genomes Genetics

Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

(2018) Johnny H. Hu et al.   NATURE

Directed evolution of CRISPR-Cas9 to increase its specificity

(2018) Jungjoon K. Lee et al.   Nature Communications

Chromatin accessibility and guide sequence secondary structure affect CRISPR-Cas9 gene editing efficiency

(2017) Kristopher Torp Jensen et al.   FEBS LETTERS

The Revolution Continues: Newly Discovered Systems Expand the CRISPR-Cas Toolkit

(2017) Karthik Murugan et al.   MOLECULAR CELL

Enhanced proofreading governs CRISPR–Cas9 targeting accuracy

(2017) Janice S. Chen et al.   NATURE

Efficient genome editing in the mouse brain by local delivery of engineered Cas9 ribonucleoprotein complexes

(2017) Brett T Staahl et al.   NATURE BIOTECHNOLOGY

In trans paired nicking triggers seamless genome editing without double-stranded DNA cutting

(2017) Xiaoyu Chen et al.   Nature Communications

The Chromatin Structure Differentially Impacts High-Specificity CRISPR-Cas9 Nuclease Strategies

(2017) Xiaoyu Chen et al.   Molecular Therapy-Nucleic Acids

Efficient Recreation of t(11;22) EWSR1-FLI1 + in Human Stem Cells Using CRISPR/Cas9

(2017) Raul Torres-Ruiz et al.   Stem Cell Reports

Factors influencing the nuclear targeting ability of nuclear localization signals

(2016) Yanping Sun et al.   JOURNAL OF DRUG TARGETING

Structural Biology and Regulation of Protein Import into the Nucleus

(2016) Mary Christie et al.   JOURNAL OF MOLECULAR BIOLOGY

Engineered Viruses as Genome Editing Devices

(2016) Xiaoyu Chen et al.   MOLECULAR THERAPY

Gene Insertion Into Genomic Safe Harbors for Human Gene Therapy

(2016) Eirini P Papapetrou et al.   MOLECULAR THERAPY

CRISPR-mediated Genome Editing Restores Dystrophin Expression and Function in mdx Mice

(2016) Li Xu et al.   MOLECULAR THERAPY

High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects

(2016) Benjamin P. Kleinstiver et al.   NATURE

Probing the impact of chromatin conformation on genome editing tools

(2016) Xiaoyu Chen et al.   NUCLEIC ACIDS RESEARCH

Selection-free gene repair after adenoviral vector transduction of designer nucleases: rescue of dystrophin synthesis in DMD muscle cell populations

(2016) Ignazio Maggio et al.   NUCLEIC ACIDS RESEARCH

The Impact of Chromatin Dynamics on Cas9-Mediated Genome Editing in Human Cells

(2016) René M. Daer et al.   ACS Synthetic Biology

Adenoviral vectors encoding CRISPR/Cas9 multiplexes rescue dystrophin synthesis in unselected populations of DMD muscle cells

(2016) Ignazio Maggio et al.   Scientific Reports

Engineered CRISPR-Cas9 nucleases with altered PAM specificities

(2015) Benjamin P. Kleinstiver et al.   NATURE

Efficient delivery of nuclease proteins for genome editing in human stem cells and primary cells

(2015) Jia Liu et al.   Nature Protocols

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Dynamics of CRISPR-Cas9 genome interrogation in living cells

(2015) Spencer C. Knight et al.   SCIENCE

Genome editing at the crossroads of delivery, specificity, and fidelity

(2015) Ignazio Maggio et al.   TRENDS IN BIOTECHNOLOGY

Improved Cell-Penetrating Zinc-Finger Nuclease Proteins for Precision Genome Engineering

(2015) Jia Liu et al.   Molecular Therapy-Nucleic Acids

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

(2014) Hiroshi Nishimasu et al.   CELL

Construction and characterization of adenoviral vectors for the delivery of TALENs into human cells

(2014) Maarten Holkers et al.   METHODS

Guide RNA Functional Modules Direct Cas9 Activity and Orthogonality

(2014) Alexandra E. Briner et al.   MOLECULAR CELL

Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease

(2014) Carolin Anders et al.   NATURE

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

(2014) Xuebing Wu et al.   NATURE BIOTECHNOLOGY

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

(2014) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation

(2014) M. Jinek et al.   SCIENCE

Adenoviral vector delivery of RNA-guided CRISPR/Cas9 nuclease complexes induces targeted mutagenesis in a diverse array of human cells

(2014) Ignazio Maggio et al.   Scientific Reports

Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System

(2013) Baohui Chen et al.   CELL

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

(2013) Prashant Mali et al.   NATURE BIOTECHNOLOGY

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Mechanism of Eukaryotic RNA Polymerase III Transcription Termination

(2013) S. Nielsen et al.   SCIENCE

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

RNA-programmed genome editing in human cells

(2013) Martin Jinek et al.   eLife

Development of an AdEasy-based system to produce first- and second-generation adenoviral vectors with tropism for CAR- or CD46-positive cells

(2012) Josephine M. Janssen et al.   JOURNAL OF GENE MEDICINE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Serum-free transient protein production system based on adenoviral vector and PER.C6 technology: High yield and preserved bioactivity

(2007) M.J.E. Havenga et al.   BIOTECHNOLOGY AND BIOENGINEERING