期刊
FUNGAL BIOLOGY
卷 124, 期 5, 页码 490-500出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.funbio.2020.01.007
关键词
Melanin production; Plant pathogen; Resting structure; Transcription factor; Verticillium transcription activator of adhesion 1 (Vta1); Verticillium spp.
类别
资金
- Deutsche Forschungsgemeinschaft (DFG) [BR1502-15-1]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2018/20571-6]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) [88881.289327/2018-01]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [18/20571-6] Funding Source: FAPESP
Many fungi are able to produce resting structures, which ensure survival and protect them against various stresses in their habitat such as exposure to UV light, temperature variations, drought as well as changing pH and nutrient conditions. Verticillium dahliae is a plant pathogenic fungus that forms melanized resting structures, called microsclerotia, for survival of time periods without a host. These highly stress resistant microsclerotia persist in the soil for many years and are therefore problematic for an effective treatment of the fungus. The Verticillium transcription activator of adhesion 1 (Vta1) was initially identified as one of several transcriptional regulators that rescue adhesion in non-adhesive Saccharomyces cerevisiae cells. Vta2 and Vta3 are required for early steps in plant infection and colonization and additionally control microsclerotia formation. Here, we show that Vta1 function is different, because it is dispensable for root colonization and infection. Vta1 is produced in the fungal cell during microsclerotia development. Analysis of the deletion mutant revealed that the absence of Vta1 allows microsclerotia production, but they are colorless and no more melanized. Vta1 is required for melanin production and activates transcription of melanin biosynthesis genes including the polyketide synthase encoding PKS1 and the lactase LAC1. The primary function of Vta1 in melanin production is important for survival of microsclerotia as resting structures of V. dahliae. (C) 2020 Published by Elsevier Ltd on behalf of British Mycological Society.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据