Review
Plant Sciences
Jeremy Astier, Jordan Rossi, Pauline Chatelain, Agnes Klinguer, Angelique Besson-Bard, Claire Rosnoblet, Sylvain Jeandroz, Valerie Nicolas-Frances, David Wendehenne
Summary: The study of nitric oxide in embryophytes and algae has deepened our understanding of its biological functions, including its production pathways, signaling networks, and regulated proteins. Interestingly, while some algae have a conserved nitric oxide synthase, the typical NO-cyclic GMP signaling module is not found in algae or embryophytes, showing a divergent acquisition of NO signaling between green and animal lineages.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Nutrition & Dietetics
Barbora Piknova, Ji Won Park, Samantha M. Thomas, Khalid J. Tunau-Spencer, Alan N. Schechter
Summary: Nitric oxide (NO) (co)regulates physiological processes in the body and its availability is affected by aging. Aging leads to changes in nitrate and nitrite contents in tissues, with higher nitrate levels and lower nitrite levels in old rats compared to young rats. However, the nitrate reduction pathway is not affected by aging. Further investigation is needed to understand the changes in NO accessibility during aging.
Article
Pharmacology & Pharmacy
Rose I. M. Batista, Renato C. Nogueira, Graziele C. Ferreira, Gustavo H. Oliveira-Paula, Lucas C. Pinheiro, Jose E. Tanus-Santos
Summary: Supplementation with L-arginine has been shown to have beneficial effects in hypertension by increasing nitric oxide formation and enhancing nitrate concentrations, but these effects may be prevented by antiseptic mouthwash. Alteration of the nitrate cycle in the body by mouthwash can impact endothelial dysfunction, antihypertensive effects, and antioxidant effects of L-arginine in hypertensive rats. The findings suggest a new mechanism where antibacterial mouth rinse can affect arterial blood pressure and the development of cardiovascular diseases.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Tijana Suboticki, Olivera Mitrovic Ajtic, Dragoslava Djikic, Marijana Kovacic, Juan F. Santibanez, Milica Tosic, Vladan P. Cokic
Summary: In various systems, hydroxyurea has been shown to stimulate the release of nitric oxide or activate nitric oxide synthase. Through inhibiting nitric oxide metabolites, hydroxyurea is able to inhibit the growth of mature erythroid colonies and impact the presence of iNOS immunoreactive CFU-E.
Review
Pharmacology & Pharmacy
Kayleigh Griffiths, Jordan J. Lee, Michael P. Frenneaux, Martin Feelisch, Melanie Madhani
Summary: Nitric oxide plays a crucial role in protecting the heart from ischemia reperfusion injury through the 'canonical' NO-sGC-cGMP pathway. A new nitrate-nitrite-NO pathway has been discovered to improve endothelial dysfunction, protect the myocardium and reduce infarct size. Nitrite itself also provides multi-faceted protection independent of nitric oxide against various pathophysiologies of IRI.
PHARMACOLOGY & THERAPEUTICS
(2021)
Article
Food Science & Technology
Raul Bescos, Mark L. Rollason, Tanisha S. Davies, Patricia Casas-Agustench
Summary: This study analyzed the content of nitrate (NO3-) and nitrite (NO2-) in commercial and self-made beetroot juices (BJs), finding that the levels of NO3- can vary with different seasons and storage conditions. The self-made BJ showed complete degradation of NO3- after 3 days at 20 degrees C, but this degradation was attenuated when stored at lower temperatures or with the addition of lemon juice. Additionally, self-made BJs had higher concentrations of NO2- and a higher pH compared to commercial BJs.
FOOD SCIENCE & NUTRITION
(2023)
Article
Cell Biology
Florentin-Daniel Staicu, Analuce Canha-Gouveia, Cristina Soriano-Ubeda, Juan Carlos Martinez-Soto, Evdochia Adoamnei, Jorge E. Chavarro, Carmen Matas
Summary: The levels of nitrite and nitrate in follicular fluid were found to be related to the yield of MII oocytes, and nitrate levels showed a suggestive inverse correlation with embryos with high potential of implantation. This suggests that nitrite and nitrate concentrations in follicular fluid may be useful in predicting ovarian response and embryo implantation potential.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Clinical Neurology
Yicong Wang, Weiqi Chen, Jian Zhou, Yongjun Wang, Hao Wang, Yilong Wang
Summary: This review aims to describe the relationship between nitrate metabolism and cerebrovascular disease, providing a basis for further advances in laboratory and clinical medicine.
FRONTIERS IN NEUROLOGY
(2022)
Article
Nutrition & Dietetics
Gary D. Miller, Beverly A. Nesbit, Daniel B. Kim-Shapiro, Swati Basu, Michael J. Berry
Summary: This study aimed to investigate the acute response of plasma nitrate and nitrite levels in healthy middle- to older-aged adults to beetroot juice alone and in combination with vitamin C or protein. The results showed no significant differences in plasma nitrate and nitrite levels at 1 h and 3 h after co-ingesting beetroot juice with vitamin C or a whey protein supplement compared to beetroot juice alone.
Article
Physiology
Michaela L. Sundqvist, Jon O. Lundberg, Eddie Weitzberg, Mattias Carlstrom
Summary: The study aimed to investigate potential sex differences in renal nitrate handling during low and high dietary nitrate intake. It was hypothesized that renal clearance and excretion of nitrate are higher in men compared to women. Results showed that women had lower urinary nitrate concentration, amount of nitrate excreted, renal nitrate clearance, and fractional excretion of nitrate compared to men.
Article
Biochemistry & Molecular Biology
Samuel J. Gentle, Khandaker A. Ahmed, Nengjun Yi, Casey D. Morrow, Namasivayam Ambalavanan, Charitharth Lal, Rakesh P. Patel
Summary: The study found that nitrate reductase activity in the oral cavity peaked at 29 weeks' post menstrual age, and infants who developed bronchopulmonary dysplasia (BPD) had significantly lower nitrate reductase activity at this time. The oral microbiota and nitrate reductase activity may play a role in the development of BPD in extremely preterm infants.
Review
Biochemistry & Molecular Biology
Zahra Bahadoran, Parvin Mirmiran, Khosrow Kashfi, Asghar Ghasemi
Summary: The lack of beneficial metabolic effects of inorganic NO3- in patients with T2DM may be attributed to species differences in AA metabolism and abnormal AA metabolism in patients with T2DM. Supplementation of AA may be needed for T2DM patients to attain the benefits of inorganic NO3- therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Physiology
Lauren K. Park, Andrew R. Coggan, Linda R. Peterson
Summary: Despite advances, heart failure with reduced ejection fraction (HFrEF) remains a deadly and disabling disease. Exercise capacity is closely linked to survival in HFrEF patients, and impaired nitric oxide (NO) bioavailability plays a key role in skeletal muscle pathology. Ingestion of inorganic nitrate has shown potential in improving exercise performance for HFrEF patients.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Matthew D. Godwin, Anu Aggarwal, Zachary Hilt, Shalini Shah, Joshua Gorski, Scott J. Cameron
Summary: A diet rich in nitrate may benefit cardiovascular health by increasing platelet NO production, with a more pronounced effect in women compared to men.
JACC-BASIC TO TRANSLATIONAL SCIENCE
(2022)
Review
Nutrition & Dietetics
Kalina Sikorska-Zimny, Luciano Beneduce
Summary: Glucosinolates and their derivatives are secondary plant metabolites abundant in Brassicaceae, with benefits for human health such as anti-inflammatory and anti-cancer effects. However, their formation requires enzymatic reactions catalyzed by myrosinase enzyme. The human gut microbiome can provide myrosinase activity, potentially enhancing the beneficial effects of these compounds.
Article
Surgery
BRITISH JOURNAL OF SURGERY
(2020)
Article
Surgery
S. J. Chapman
BRITISH JOURNAL OF SURGERY
(2020)
Article
Biochemistry & Molecular Biology
Joao Laranjinha, Carla Nunes, Ana Ledo, Catia Lourenco, Barbara Rocha, Rui M. Barbosa
Summary: This review explores the regulatory and toxic role of (NO)-N-center dot in multiple pathways, including its potential impact on Parkinson's disease and cell death processes. Additionally, it briefly discusses the modulatory role of NO in brain energy metabolism and as a key regulator of neurovascular responses. Lastly, it introduces a new pathway, the nitrate:nitrite:NO pathway, providing new perspectives on NO biology.
NEUROCHEMICAL RESEARCH
(2021)
Article
Gastroenterology & Hepatology
COLORECTAL DISEASE
(2020)
Article
Neurosciences
Matilde S. Rodrigues, Claudia Ferreira, Candida Dias, Anna Pliassova, Lisiane Souza, Ana Ledo, Joao Laranjinha, Rodrigo A. Cunha, Attila Kofalvi
Summary: In this study, a simple complementary ex vivo technique for quantifying the activity of key enzymes involved in the production and degradation of 2-AG in brain samples was optimized. The results showed that this technique has potential applications in assessing changes in 2-AG production in neuropsychiatric disorders and health condition models.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Carla Nunes, Joao Laranjinha
Summary: The molecular mechanisms underlying Parkinson's disease are not fully understood, but mitochondrial dysfunction and nitroxidative stress have been identified as major contributors to dopaminergic neuron degeneration. Excessive nitric oxide and abnormal dopamine metabolism are also factors, suggesting a potential pathway for neurotoxicity. This review highlights the connection between abnormal dopamine and nitric oxide metabolism, suggesting a mechanism involving mitochondrial dysfunction, glutathione depletion and nitroxidative stress in dopaminergic cell death.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Neurosciences
Gianni Mancini, Candida Dias, Catia F. Lourenco, Joao Laranjinha, Andreza de Bem, Ana Ledo
Summary: The study found that a high fat/cholesterol diet may lead to mitochondrial dysfunction and significantly decreased nitric oxide production in a mouse model of Alzheimer's disease, exacerbating brain tissue damage. In normal mice, the diet only caused moderate hypercholesterolemia but resulted in significant decline in spatial memory.
JOURNAL OF ALZHEIMERS DISEASE
(2021)
Article
Psychiatry
Ines A. Trindade, Joana Pereira, Ana Galhardo, Nuno B. Ferreira, Paola Lucena-Santos, Sergio A. Carvalho, Sara Oliveira, David Skvarc, Barbara S. Rocha, Francisco Portela, Claudia Ferreira
Summary: This study aims to test the efficacy of a psychological intervention called LIFEwithIBD in improving psychological distress, quality of life, and social functioning. The trial includes 61 participants randomized into an experimental group and a control group, with results expected to support future research on interventions for IBD.
FRONTIERS IN PSYCHIATRY
(2021)
Review
Physiology
Catia F. Lourenco, Joao Laranjinha
Summary: The brain relies heavily on continuous blood supply to meet its energy demands, with the coupling between neuronal activity and cerebral blood flow supported by neurovascular coupling. Nitric oxide (NO) is recognized as a key player in this process, promoting vasodilation and essential for the development of the neurovascular response.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
A. Ledo, E. Fernandes, A. Salvador, J. Laranjinha, R. M. Barbosa
Summary: Hydrogen peroxide is a significant redox signaling molecule that plays a crucial role in cell function and communication. In the brain, it acts as a signaling molecule and neuromodulator. Our study reveals that hydrogen peroxide can diffuse in the extracellular space and has a relatively short half-life. These quantitative details provide valuable insights into the physiology of redox signaling and lay the foundation for studying dysregulation in redox homeostasis associated with diseases.
Review
Biochemistry & Molecular Biology
Cesar G. Fraga, Andres Trostchansky, Barbara S. Rocha, Joao Laranjinha, Homero Rubbo, Monica Galleano
Summary: Certain plant polyphenols regulate the production of nitric oxide enzymes, while nitrolipids can increase the bioavailability of nitric oxide. These molecules play a significant role in maintaining human homeostasis, especially under inflammatory conditions.
MOLECULAR ASPECTS OF MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Katerina Reva, Joao Laranjinha, Barbara S. Rocha
Summary: Diet is recognized as a major factor that influences human health. The gut microbiota plays a crucial role in various physiological functions and produces metabolites that can affect the host. Recent studies have shown that gut microbiota metabolites can induce epigenetic modifications, linking diet, microbiota, and health. Understanding the molecular mechanisms underlying microbiota-host interactions and epigenetic changes induced by diet is opening up new avenues for personalized therapeutic strategies.
Review
Biochemistry & Molecular Biology
A. Ledo, C. F. Lourenco, E. Cadenas, R. M. Barbosa, J. Laranjinha
Summary: Nitric oxide is a biological ubiquitous signaling molecule with unique physical chemical properties, playing important roles in brain physiology, aging, and neurodegeneration. Understanding the concentration dynamics of nitric oxide in the brain is crucial for studying neurovascular coupling and bioenergetic metabolism.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)