期刊
FOOD CONTROL
卷 107, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2019.106761
关键词
Upconversion nanoparticles; Aptamers; Fluorescence; Escherichia coli; Food
资金
- National Natural Science Foundation of China [31772063]
- key R&D Program of Ningxia Hui Autonomous Region [2018BCF1001]
- key R&D Program of Jiangsu Province [BE2017357]
- China Postdoctoral Science Foundation [2019M651748]
- Beijing Key Laboratory of Big Data Technology for Food Safety, Beijing Technology and Business University [BTBD-2019KF09]
- National Key Research and Development Program of China [2017YFC1600801]
The study assembles a highly sensitive upconversion fluorescence sensor for screening Escherichia coli (E. coli) in food. The principle of the strategy was based on the specific matching between two aptamers embedded magnetic nanoparticles (MNPs) and cDNA-upconversion nanoparticles (UCNPs). The fluorescence emission intensity of the MNPs-aptamer-cDNA-UCNPs conjugate system gradually decreased upon rise in the E colt levels. This change was regarded as a concentration equivalent for E coli at 662 nm. The morphology, crystallinity, vibrational and electronic characterization for the formed MNPs-aptamer-eDNA-UCNPs conjugate were safely assigned. Under the optimum conditions, the fluorescence sensor achieved a lower limit of detection (10 cfu mL(-1) in the linear range of 58-58 x 10(6) cfu mL(-1). Furthermore, the applicability of the proposed sensor to detect E coli was examined in adulterated pork samples. The strategy was also validated by a standard plate count method with satisfactory recovery (p > 0.05).
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