Article
Ophthalmology
Yasushi Kitaoka, Kana Sase, Chihiro Tsukahara, Naoki Fujita, Ibuki Arizono, Jiro Kogo, Naoto Tokuda, Hitoshi Takagi
Summary: This study investigated the effect of netarsudil on TNF-induced axon loss and found that it increased autophagy and protected against axon degeneration.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Abdalla Elbialy
Summary: Autophagy is a crucial pathway for maintaining cellular homeostasis through recycling damaged proteins and organelles, with LC3 protein levels often measured by immunofluorescence or western blot analysis. This study demonstrates that 3D image analysis can be used as a quantitative, rapid tool for monitoring autophagy in zebrafish larvae and avoiding drawbacks of LC3 western blot analysis. Using a three-dimensional approach allows for accurate quantification of autophagy from LC3 and/or P62 immunostaining images in zebrafish larvae.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Microbiology
Zhaodi Wang, Yukang Wen, Bingqian Zhou, Yaqin Tian, Yaru Ning, Honglei Ding
Summary: The study demonstrated that Mycoplasma hyopneumoniae infection increases autophagic vacuoles in host cells, with enhanced expressions of autophagy marker proteins. Accumulation of autophagosomes promotes the expression of P97 protein and enhances the survival of M. hyopneumoniae in host cells. Inhibition of autophagosome formation reduces the replication of M. hyopneumoniae.
JOURNAL OF MICROBIOLOGY
(2021)
Article
Plant Sciences
Weiwei Zhang, Xu Geng, Qing Dong, Xiuhan Li, Ping Ye, Mengyuan Lin, Bin Xu, Hong Jiang
Summary: This study investigated the mechanism of realgar-induced neurotoxicity and its effects on autophagy and the Keap1-Nrf2-ARE pathway. The results showed that arsenic from realgar accumulated in the brain and blood, leading to neuronal and synaptic damage, decreased exploratory behavior and spontaneous movement, and impaired memory ability in rats. The mechanism may involve realgar-mediated autophagy impairment and continuous activation of the Nrf2 pathway. However, the activation of the Nrf2 pathway was insufficient to counteract oxidative damage, resulting in aggravated apoptosis in the cerebral cortex.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Oncology
Dongqi Li, Chuanchun He, Fan Ye, En Ye, Hao He, Gong Chen, Jing Zhang
Summary: The overexpression of p62 in lung adenocarcinoma bone metastasis is significantly associated with more bone lesions, shorter overall survival rates, and shorter progression free survival rates. In vitro experiments showed that downregulation of p62 inhibited cell migration but not cell proliferation. The expression of p62 in autophagy flux changed inconsistently according to the switch of LC3I to LC3II in different autophagy conditions.
FRONTIERS IN ONCOLOGY
(2021)
Article
Fisheries
Liming Xu, Jingzhuang Zhao, Miao Liu, Guangming Ren, Shi Cheng Han, Yizhi Shao, Yongsheng Cao, Hongbai Liu, Tongyan Lu
Summary: IHNV enhances the multiplication of IPNV during co-infection, with the enhancement becoming stronger with increasing infection time interval, regardless of the infection order.
Article
Ophthalmology
Kana Sase, Chihiro Tsukahara, Naoki Fujita, Ibuki Arizono, Mizuki Otsubo, Yasushi Kitaoka
Summary: The purpose of this study is to investigate the relationship between p38 and autophagy, and to determine whether an inhibitor of p38 can prevent axon loss induced by TNF and affect autophagy. The results show that the p38 inhibitor can significantly protect against TNF-induced axon loss and promote autophagy expression.
INTERNATIONAL OPHTHALMOLOGY
(2023)
Review
Biology
Akitoshi Nakashima, Atsushi Furuta, Kiyotaka Yamada, Mihoko Yoshida-Kawaguchi, Akemi Yamaki-Ushijima, Ippei Yasuda, Masami Ito, Satoshi Yamashita, Sayaka Tsuda, Satoshi Yoneda, Shibin Cheng, Surendra Sharma, Tomoko Shima
Summary: This review introduces studies related to autophagy in the field of reproductive health and provides tips for interpreting experimental results and understanding autophagy. Autophagy is a fundamental process involved in regulating cellular homeostasis, energy production, protein degradation, and maintenance of organelle function. Accurate evaluation of autophagy activity is difficult in fixed tissues, including human samples.
Article
Cell Biology
Cenxiao Fang, Jung-A A. Woo, Tian Liu, Xingyu Zhao, Sara Cazzaro, Yan Yan, Jenet Matlack, Teresa Kee, Patrick LePochat, David E. Kang
Summary: The study reveals that SSH1, a canonical CFL phosphatase, dephosphorylates phospho-Ser403-SQSTM1, impairing SQSTM1 flux and phospho-MAPT/tau clearance. This unique action of SSH1 on SQSTM1 is separate from CFL activation and suggests an inhibitory role of SSH1 in autophagic cargo clearance.
Article
Biochemistry & Molecular Biology
Guo-En Wang, Xiao-Ting Liu, Fan Yang, Ruo-Hong Wang, Xin-Yu Liu, Xi-Ting Lv, Xiao-Li Lin, Yan-Fen Chen
Summary: This study demonstrated the antihepatosteatosis effect of biochanin A (Bio-A) on oleate (OA)-treated hepatocytes by activating SIRT3/AMPK/ULK-1-mediated autophagy, providing a theoretical basis for the application of Bio-A to hepatic steatosis-related diseases.
JOURNAL OF FOOD BIOCHEMISTRY
(2022)
Article
Oncology
Dongqing Hu, Yanming Huo, Ye Xue, Haixia Feng, Wei Sun, Huiqi Wang, Jing Wu, Xiaoyan Wang
Summary: This study evaluated the prognostic value of autophagy proteins in colorectal cancer (CRC) and found that Beclin-1, LC3B, and p62 may serve as promising prognostic biomarkers for CRC. Additionally, high expression of LC3B and p62 can be reliable tools for predicting tumor metastasis.
Article
Cell Biology
John M. Barbaro, Ana Maria Cuervo, Joan W. Berman
Summary: HIV infection in the CNS early on creates reservoirs, opioid use may worsen neurocognitive symptoms in PWH, and macroautophagy helps maintain macrophage function to reduce HAND.
Article
Clinical Neurology
Yang Huang, Hao Ren, Xiang Gao, Danyang Cai, Huajian Shan, Jinyu Bai, Lei Sheng, Yong Jin, Xiaozhong Zhou
Summary: This study aimed to investigate the effects of amlodipine on spinal cord injury and autophagy. The results showed that amlodipine inhibited motor neuronal apoptosis by upregulating autophagy, promoting spinal cord injury repair.
Article
Biochemistry & Molecular Biology
Yuanchang Zhu, Zhiqin Zhang, Zhuanhong Ma, Weifen Deng, Yaou Zhang, Qiongfang Wu
Summary: This study found an association between autophagy and recurrent implantation failure (RIF), suggesting that autophagy may be involved in the pathogenesis of RIF.
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2022)
Article
Environmental Sciences
Robyn L. Bilski, Joseph M. Wheaton, Joseph E. Merz
Summary: This research investigates the impact of in-channel structure on the hydraulics and hyporheic flow of a river, and examines the relationship between these changes and the survival, development, and growth of Chinook salmon embryos. The study finds that in-channel structure disrupts water velocity patterns and creates pressure differences, leading to increased vertical hydraulic gradients within the subsurface. While overall there is no significant increase in the survival, development, and growth of Chinook salmon embryos, certain downstream areas with low gradients and in-channel structure show significantly higher rates of survival, development, and growth.