Article
Chemistry, Analytical
Yan Zhao, Li Yuan, Xiao-Lin Bai, Xin-Xin Jiang, Yi Zhang, Qiong Fang, Qin Zhang, Xun Liao
Summary: In this study, tyrosinase was immobilized on carboxyl functionalized silica-coated magnetic nanoparticles for the first time to fish tyrosinase's ligands from complex plant extract. The immobilized tyrosinase exhibited high reusability and thermostability compared to its free form. Two tyrosinase's ligands, caffeic acid and rosmarinic acid, were successfully extracted from Prunellae Spica extract using the immobilized tyrosinase. The proposed method, combining magnetic separation and ligand fishing, has great potential for screening bioactive compounds from complex matrices.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Environmental Sciences
Kavita Sharma, Preeti Tewatia, Manpreet Kaur, Deepak Pathania, Fawzi Banat, Gaurav Rattan, Sonal Singhal, Anupama Kaushik
Summary: An easily recyclable biocatalyst called Lac@CDI-MCNFs was synthesized by immobilizing laccase on rice straw-derived carbonyldiimidazole mediated magnetized cellulose nanofibers (MCNFs). Lac@CDI-MCNFs were used for bioremediation of cefixime antibiotic (CT), carbofuran pesticide (CF) and safranin O dye (SO) in wastewater through oxidation-reduction reactions. The Lac@CDI-MCNFs showed high stability and could be reused for up to 25 cycles with only a 20% decline in effectiveness. They effectively degraded 98.2% CT and 96.8% CF within 20 hours, as well as completely degrading SO in just 7 hours.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Applied
Shasha Li, Liangzhi Qiao, Chao Liang, Liangshen Zhao, Kaifeng Du
Summary: High-strength self-reinforced all-cellulose microspheres (SCMs) were fabricated using cellulose nanofibers (CNFs) as fillers, which possessed excellent selectivity. The combination with stiff CNFs enabled efficient stress transfer during the separation process, and the homogeneous surface chemistry and pore structure characteristics made SCMs a more suitable adsorbent material.
CARBOHYDRATE POLYMERS
(2022)
Article
Chemistry, Physical
Siyuan Lu, Hongyi Tu, Yangdong Liu, Boyuan Zhang, Zhenbin Chen
Summary: A Mn2+-Fe3O4@PTA-GMA-g-RAFT NPs carrier with high catalytic activity was successfully prepared through a series of chemical reactions, and efficient and stable immobilization of PGA enzyme was achieved. The experimental results showed that the carrier exhibited high catalytic activity, stability, and recyclability, indicating its potential for biomedical applications.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Biochemistry & Molecular Biology
Hongcai Zhang, Tingting Qiu, Yifei Bai, Bin Chen, Juan Yan, Jianxiong Xu
Summary: In this study, cellulose nanocrystals were used to stabilize Lysozyme encapsulated in quaternary ammonium chitosan nanoparticles. The CNC stabilized Lys loaded QC NPs showed potential implications in the food industry for food preservation and packaging, as well as demonstrated good antibacterial activity against Staphylococcus aureus and Vibrio parahaemolyticus.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Applied
Simei Wu, Yaqi Gong, Shilin Liu, Ying Pei, Xiaogang Luo
Summary: Functionalized phosphorylated cellulose microspheres (CMP) were designed as a carrier for ciprofloxacin (CIP) drug delivery system, successfully incorporating and releasing CIP with a significant antibacterial effect. This study provides insight into the design of suitable cellulose microspheres for CIP delivery.
CARBOHYDRATE POLYMERS
(2021)
Article
Polymer Science
Diana Gago, Marta C. Corvo, Ricardo Chagas, Luisa M. Ferreira, Isabel Coelhoso
Summary: Dicarboxymethyl cellulose (DCMC) was synthesized and tested for protein adsorption. The results showed that DCMC has a high adsorption capacity for positively charged proteins, cytochrome C and lysozyme, and the adsorption process follows the Langmuir adsorption isotherm. DCMC also exhibited good adsorption kinetics and high reuse efficiency.
Article
Chemistry, Multidisciplinary
Chuhan Lv, Xuewei Yang, Zongkang Wang, Ming Ying, Qingguo Han, Shuangfei Li
Summary: The research demonstrates that amination modification significantly improves the performance of bioelectrodes, leading to increased enzyme immobilization and activity, enhanced peak current intensity and transfer rate, as well as remarkable electro-activity and favorable reaction at the electrode.
Article
Chemistry, Physical
Yu Wang, Yue Wang, Chen Liu, Xin Yuan, Jing Peng, Jiuqiang Li, Maolin Zhai
Summary: In this work, acrylic acid was grafted onto covalent organic framework (COF) through a γ-ray radiation grafting approach, resulting in a carboxyl-functionalized adsorbent designated AA-TbDa-COF with high performance for heavy metal removal. Infrared spectroscopy and thermogravimetric analysis confirmed the successful carboxyl modification. The porous nanofiber morphology and high accessibility of carboxyl functional groups contribute to the ultra-fast adsorption kinetics of AA-TbDa-COF, with a decontamination capacity of 127.9 mg/g for Pb2+, 60.6 mg/g for Cd2+, and 39.8 mg/g for Cu2+. The preparation of functional COFs by radiation method demonstrates the great potential of radiation-prepared materials in environmental governance.
RADIATION PHYSICS AND CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Wenkang Li, Manman Zhang, Lifang Peng, Jifu Du, Rong Hua, Long Zhao
Summary: In this study, four nucleobases were used to modify cellulose in order to explore the structure-property relationship for adsorption of rhenium. The adsorption performance was evaluated and it was found that the type of nucleobases affected the maximum adsorption capacity. The adsorbents showed selective capture of trace rhenium from simulated uranium ore leaching solution and exhibited potential for rhenium recovery.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Engineering, Chemical
Di Wen, Cong Xie, Zhen Dong, Manman Zhang, Maolin Zhai, Long Zhao
Summary: Functionalized microcrystalline cellulose microspheres were used to prepare a selective adsorbent (t-DOPOR) for U(VI) removal. The adsorbent showed considerable selectivity towards U(VI) in static and dynamic column experiments, and was successfully applied for U(VI) removal from simulated water.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Optics
Najwa Norimanina Muhammad Rosddi, Yap Wing Fen, Nur Alia Sheh Omar, Nur Ain Asyiqin Anas, Hazwani Suhaila Hashim, Nur Syahira Md Ramdzan, Nurul Illya Mohd Fauzi, Muhammad Fahmi Anuar, Wan Mohd Ebtisyam Mustaqim Mohd Daniyal
Summary: A graphene quantum dots composite thin film-based SPR sensor was developed for glucose detection, showing potential for practical applications. The gold/CGQDs-NCC thin film exhibited high sensitivity and binding affinity in detecting glucose concentrations ranging from 0 to 10 μM.
Article
Agricultural Engineering
Lehao Lun, Yaoming Su, Xing Gong, Lijuan Zhang, Peipei Meng, Dan Peng, Qianya Zhou, Hao Zeng, Liuchun Zheng
Summary: Diethylenetriamine pentaacetic acid (DTPA) functionalized modified corn stalk cellulose (DCS) was prepared and used for the adsorption of sulfamethoxazole (SMZ) wastewater in the presence of copper (Cu). DCS exhibited hydrophilic groups, larger contact space, and lower steric hindrance, compared to raw corn stalk cellulose (RCS). The adsorption amounts for Cu and SMZ in the binary system were 4.41 mg/g and 29.7 mg/g, respectively. DCS demonstrated good pH buffering capability, resistance to interfering ions, and excellent regeneration performance. Competitive adsorption and complexation synergy occurred between Cu, SMZ, and DCS. Density functional theory (DFT) calculations further explained the adsorption mechanism, showing that DCS had more SMZ adsorption sites and could provide additional adsorption sites for Cu or SMZ.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Chemistry, Multidisciplinary
Xin Zhang, Jing Guo, Yue Yu, Xinmin Hao, Fucheng Guan, Sen Zhang
Summary: A cleaning and reusable biochemical degumming method using calcium alginate microspheres loaded with pectinase lyase (CA-PL) was proposed in this study. The method achieved complete degumming in a shorter time and at a lower temperature. The results showed that the biochemical degumming solution could be reused three times with high cellulose extraction rates and breaking strength. Repeating the method two times was considered optimal for better degumming efficiency and cellulose extraction degree.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Environmental Sciences
Zhaolin Du, Hongan Chen, Xiaoyan Guo, Li Qin, Dasong Lin, Lili Huo, Yanpo Yao, Zhihao Zhang
Summary: The study successfully prepared a novel adsorbent RSMW-AC through microwave-assisted approach and demonstrated its excellent adsorption performance towards heavy metal ions in water. By optimizing process conditions and exploring the adsorption mechanisms, it showed potential for industrial applications in treating heavy metal-contaminated water.
Article
Biotechnology & Applied Microbiology
Thore Bach Thomsen, Tobias S. Radmer, Anne S. Meyer
Summary: Plastic pollution, particularly from PET, is a significant environmental challenge. This study investigates the enzyme kinetics of PET degradation and finds that the degree of crystallinity (X-C) has a substantial impact on the enzymatic reaction rate. The mobile amorphous fraction (X-MAF) is a better descriptor for the rate of enzymatic degradation. The longer lag phase observed with increased X-C is proposed to be due to increased substrate entanglement density and unproductive enzyme binding to the crystalline regions of PET.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Purna Bahadur Poudel, Dipesh Dhakal, Jong Cheol Lee, Jae Kyung Sohng
Summary: Methylation plays important roles in various biological processes, and this study characterized the function of ThnM3 in regioselective modifications of 1,8-dihydroxynaphthalene.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Feng Song, Ivan Birukou, Mark Bednarcik, Scott Young
Summary: In this study, a highly sensitive and specific method using ultra performance liquid chromatography (UPLC) was developed to measure the enzymatic activity of APH4 in plants. The method exhibited good precision and accuracy, and was validated for use with samples from different sources.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Yuan Rao, Wei Zhuang, Jinle Liu, Ting Tang, Zhi Wang, Hanjie Ying
Summary: This study describes a mild and versatile strategy to immobilize glucose oxidase (GOD) on carboxyl-activated metal-organic framework (MOF) through DNA-directed immobilization technology. The immobilized GOD showed enhanced stability and catalytic activity, making it a potential candidate for applications in biocatalysis and biotechnology.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Julen Ordenana Manso, Martin B. Nielsen, Eva Balaguer Moya, Juliana P. Sandri, Celina K. Yamakawa, Solange I. Mussatto
Summary: This study focuses on the development of an enzymatic cocktail for the conversion of corn fiber into monosaccharides. The results reveal that the recalcitrance and unique chemical composition of corn fiber require a tailored enzymatic cocktail for degradation, and attention should also be given to the pretreatment step.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Cuixia Zhou, Guangcheng Yang, Panpan Meng, Weishuai Qin, Yanyan Li, Zhenxian Lin, Wei Hui, Huitu Zhang, Fuping Lu
Summary: Bacillus licheniformis 2709 is an important industrial producer of alkaline protease (AprE), and this study identified the UP elements of the aprE gene and demonstrated the lack of significant effect of CggR, FruR, and YhcZ transcription factors on aprE expression. Enhanced aprE expression and enzyme activity were achieved by deleting a negative regulatory factor binding site in the genome. These findings provide valuable insights into the high-level production of proteins in other Bacillus species.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Xinqi Xu, Tianheng Chen, Lian Xu, Juan Lin
Summary: In this study, laccase was immobilized on modified magnetic nanoparticles, improving the enzyme's thermostability and efficiency for polymerization of phenols.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Handan Ustukarci, Gul Ozyilmaz, Ali Tuncay Ozyilmaz
Summary: A biofilm layer called biofouling is formed over time by organic molecules and microorganisms on solid surfaces immersed in a liquid medium. This study focused on the antifouling performance of polyaniline (PANI) and investigated the immobilization of hydrolytic enzymes in PANI coatings. The results showed that the alpha-amylase-containing film exhibited the highest antifouling activity. Surface properties and biofilm composition were analyzed to support these findings.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)
Article
Biotechnology & Applied Microbiology
Mariana Ortiz-Sanchez, Juan Camilo Solarte-Toro, Pablo Jose Inocencio-Garcia, Carlos Ariel Cardona Alzate
Summary: This study evaluated the sustainability of biorefineries using orange peel as a raw material. By applying the concept of biorefineries and experimental data, it was found that steam distillation and polyphenolic compound extraction can improve the process performance, resulting in higher yields of different products. The integrated utilization of orange peel in biorefineries showed a high sustainability index.
ENZYME AND MICROBIAL TECHNOLOGY
(2024)