Article
Food Science & Technology
Chang Liu, Juliang Huang, Yanbo Hu, Xiuli Wu
Summary: This study investigated the influence of different protease treatments on the functional properties, structural characteristics, and biological activities of deer blood hydrolysates. The hydrolysates treated with pepsin and alacase showed higher degree of hydrolysis and smaller molecular weight. Various analyses, including SEM, solubility, FTIR, secondary structure, color properties, amino acids, and antioxidant properties, were conducted. The results indicate that the choice of protease significantly impacts the properties and activities of the hydrolysates.
FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Environmental Sciences
Fadoua Jabeur, Sondes Mechri, Fethi Mensi, Ines Gharbi, Yosri Ben Naser, Mouna Kriaa, Nejla Bejaoui, Samir Bachouche, Abdelmalek Badis, Rachid Annane, Mostapha Djellali, Saloua Sadok, Bassem Jaouadi
Summary: In this study, a co-culture of three protease-producing bacterial isolates was carried out to optimize protease production using blue swimming crab bio-waste. Valuable products, including protein hydrolysate and chitosan with antimicrobial activity, were obtained.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Materials Science, Textiles
Entesar A. Hassan, Hamada M. Mashaly, Zeinab M. Hashem, Salem E. Zayed, Ahmed M. Abo-Bakr
Summary: A series of heterocyclic compounds containing azo function were synthesized and treated with different reagents to form various samples. The dyeing properties, light fastness, washing fastness, and perspiration fastness of these samples were studied, and the results showed that these materials have good resistance to rubbing, washing, and thermal fixation. In addition, their biological activities were screened.
FIBERS AND POLYMERS
(2022)
Article
Biochemistry & Molecular Biology
Ana Maria Sixto-Berrocal, Marlenne Vazquez-Aldana, Susana Patricia Miranda-Castro, M. Aurora Martinez-Trujillo, Martin R. Cruz-Diaz
Summary: In this study, two lactic bacteria were used to sequentially co-culture and demineralize and deproteinize shrimp shells in order to obtain chitin. The highest demineralization (98.63%) and deproteinization (88%) were achieved by adding a glucose pulse during demineralization and controlling the pH value above 6.0 during deproteinization. Furthermore, deacetylation of the resulting chitin was catalyzed by a deacetylases cocktail produced by Aspergillus niger. The chitosan samples obtained had a deacetylation degree higher than 78% and a solubility of 25% in 1.0 N acetic acid.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Applied
Litao Wang, Jinxia Hu, Wenjing Lv, Weihang Lu, Dong Pei, Yuanmeng Lv, Wenjing Wang, Minna Zhang, Ruifang Ding, Mei Lv
Summary: The study found that neutral protease was the best enzymatic protease for treating shrimp shells, and using XDA-8 macroporous adsorption resin could improve the yield and purity of astaxanthin while reducing production costs. This innovative technique offers a high value-added utilization of shrimp shells.
Article
Agriculture, Multidisciplinary
Jun-Hui Cheng, Wen-Xiao Zhao, Hai-Yan Cao, Zhen Wang, Yan Wang, Qi Sheng, Yin Chen, Peng Wang, Xiu-Lan Chen, Yu-Zhong Zhang
Summary: Collagen oligopeptides are widely used in various industries for their high bioactivities and bioavailability. A4095, the most abundant S8 protease secreted by Anoxybacillus caldiproteolyticus 1A02591, was characterized as a collagenolytic protease that can produce collagen oligopeptides by preferentially hydrolyzing the Y-Gly peptide bonds in denatured bovine bone collagen.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Ohood A. Amer, Sameh S. Ali, Maha Azab, Wagih A. El-Shouny, Jianzhong Sun, Yehia A. -G Mahmoud
Summary: Exploitation of shellfish waste for bioconversion can maximize the utilization of chitin-containing seafood processing wastes, producing enzymes and bioactive materials.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Medicinal
Dan-Hsin Lee, Chien Thang Doan, Thi Ngoc Tran, Van Bon Nguyen, Anh Dzung Nguyen, Chuan-Lu Wang, San-Lang Wang
Summary: In this study, a protease-producing bacterial strain Paenibacillus elgii TKU051 was isolated and shown to produce at least four proteases when grown on squid pens powder as the sole carbon/nitrogen source. The crude enzyme cocktail from P. elgii TKU051 exhibited optimal activity at pH 6-7 and 60 degrees C. Additionally, the chitin obtained from deproteinization process by P. elgii TKU051 displayed characteristics similar to commercial chitin in terms of adsorption against Congo Red, suggesting its potential in extracting chitin from chitinous fishery by-products.
Article
Infectious Diseases
Soibam Ngasotter, K. A. Martin Xavier, Layana Porayil, Amjad Balange, Binaya Bhusan Nayak, Shibu Eapen, K. J. Adarsh, M. S. Sreekala, Rupam Sharma, George Ninan
Summary: This study successfully prepared chitin nanocrystals (ChNCs) from shrimp shell chitin using steam explosion method for the first time. Response surface methodology was used to optimize the conditions, and the maximum yield achieved was 76.78%. The ChNCs produced by steam explosion had an irregular spherical shape with an average diameter of 55.70 ± 13.12 nm, slightly different from chitin in terms of peak positions and intensities in FTIR spectra, and exhibited a typical α-chitin structure according to XRD patterns. Thermal analysis showed ChNCs were less thermally stable than chitin. Compared to conventional acid hydrolysis, the steam explosion method described in this study is simpler, faster, and requires less acid concentration and quantity, making it more scalable and efficient for synthesizing ChNCs. Furthermore, the characteristics of the ChNCs have potential industrial applications.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Energy & Fuels
Merve Canpulat, Orkun Pinar, Zeynep Yilmaz-Sercinoglu, Dilek Kazan
Summary: This study investigates the deproteinization of shrimp waste and the production of alkaline protease enzyme by obligate alkalophilic Bacillus marmarensis. Optimum conditions for alkaline protease production are determined, and the highest protease activity is obtained from shrimp waste pretreated at specific conditions. B. marmarensis shows a capacity for deproteinization of shrimp waste and the production of alkaline proteases. The use of different carbon and nitrogen sources is also compared. Shrimp waste remains the best substrate for enzyme production. The results suggest that B. marmarensis can be used for sustainable bioprocesses using shrimp waste.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Chemistry, Applied
Qiao Zhang, Liuyan Wang, Shiguo Liu, Yongcheng Li
Summary: In this study, a process of chitin bio-extraction from shrimp shells powder was simplified by using successive co-fermentation with Bacillus subtilis and Acetobacter pasteurianus. The purified chitin obtained from this method showed excellent structural characteristics compared to commercial chitin, with high deacetylation degree and lower molecular weight.
CARBOHYDRATE POLYMERS
(2021)
Article
Food Science & Technology
Lei Lei, Yue-liu Chen, Cai-hong Zhu, Hao-fan Wu, Zhi-li Wan, Xin-quan Yang, Yang Yuan
Summary: The aim of this paper was to investigate the synergistic mechanism of the novel Pickering emulsion gels stabilized by zein hydrolysate-chitin nanocrystals coacervates and their improvement on the stability and bioaccessibility of curcumin. The results showed that the novel gels exhibited better rheological properties, emulsion stability, and oxidation resistance, making them potential carriers for enhancing stability, delayed lipolysis, or sustained nutrient release in foods and pharmaceuticals.
FOOD RESEARCH INTERNATIONAL
(2022)
Review
Chemistry, Applied
Yihao Liu, Zhen Qin, Chunling Wang, Zhengqiang Jiang
Summary: Chitin, the second most abundant biopolymer, has various applications in different industries. However, its high crystallinity and low solubility limit its potential uses. Two types of GlcNAc-based oligosaccharides, obtained from chitin through enzymatic methods, have lower molecular weights and improved solubility, showing more beneficial health effects compared to chitin. These oligosaccharides have antioxidant, anti-inflammatory, anti-tumor, antimicrobial, plant elicitor activities, as well as immunomodulatory and prebiotic effects, making them suitable for food additives, daily supplements, drug precursors, plant elicitors, and prebiotics.
CARBOHYDRATE POLYMERS
(2023)
Article
Engineering, Chemical
Samuel Verdu, Cristina Fuentes, Ana Fuentes, Alberto J. Perez, Jose M. Barat, Rail Grau
Summary: This study aimed to use RGB laser-scattering imaging to control the protein hydrolysis process of fish by-products. Hydrolysis of mackerel and sardine waste with papain, pepsin, and Protamex ® was measured six times over a 6-hour period. Imaging data captured at sampling times provided non-destructive information. Different enzymes achieved varying degrees of hydrolysis depending on the fish type. Multivariate regression analysis showed high predictive capacity for the degree of hydrolysis, regardless of the enzyme or fish type. A common prediction model was developed which accounted for the effect of the fish matrix, resulting in prediction coefficients up to 0.95. The results demonstrated the capability of this imaging technique to capture the variability during protease hydrolysis regardless of the enzyme or fish type.
JOURNAL OF FOOD ENGINEERING
(2023)
Article
Acoustics
Qiao Zhang, Lirui Duan, Yongcheng Li
Summary: This study investigates the effect of ultrasound on chitin extraction from shrimp shells powder (SSP) through bacterial co-fermentation. High-intensity ultrasound (800 W) pre-treatment of SSP increases protease activity in the fermentation solution and shortens the fermentation time. The ultrasound also leads to the degradation and structural damage of protein in SSP, resulting in higher chitin purity and degree of deacetylation.
ULTRASONICS SONOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Amel Dahdouh, Nawel Boucherba, Khelifa Bouacem, Sondes Mechri, Adel Amirouche, Ali Aksas, Bassem Jaouadi, Djamel Edine Kati
Summary: The study purified a plant peroxidase TRP from turnip roots and characterized its biochemical properties, revealing high catalytic efficiency and antioxidant activities, indicating its potential application in enzyme therapy for the pharmaceutical and medicinal industries.
BIOCATALYSIS AND BIOTRANSFORMATION
(2022)
Article
Biochemistry & Molecular Biology
Khelifa Bouacem, Fawzi Allala, Nadia Zarai Jaouadi, Sondes Hamdi, Sondes Mechri, Kahina Ighilahriz, Hatem Rekik, Hocine Hacene, Amel Bouanane-Darenfed, Bassem Jaouadi
Summary: A novel extracellular lignin peroxidase (LiP PR40) was produced by a white-rot Agaricomycetes fungus strain newly isolated from dead deciduous trees in Algeria. This purified enzyme showed premium activity at pH 3 and 80 degrees C, and exhibited higher catalytic efficiency and dye-decolorisation aptitude compared to commercial horseradish peroxidase and reported LiP BA45. These properties make LiP PR40 a potential candidate for destaining synthetic dyes and lignin biodegradation.
BIOCATALYSIS AND BIOTRANSFORMATION
(2022)
Article
Biochemistry & Molecular Biology
Bilal Kerouaz, Bassem Jaouadi, Alain Brans, Boudjema Saoudi, Amina Habbeche, Soumeya Haberra, Hafedh Belghith, Ali Gargroui, Ali Ladjama
Summary: Two novel extracellular keratinases were produced by Actinomadura keratinilytica strain Cpt20, purified and characterized. KERA-71 and KERB-19 exhibited different optimal activity conditions and hydrolytic efficiency, showing potential applications in valorization of keratin-containing wastes and leather industry.
PROCESS BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Sondes Mechri, Nadia Zarai Jaouadi, Khelifa Bouacem, Fawzi Allala, Aicha Bouraoui, Celine Ferard, Hatem Rekik, Alexandre Noiriel, Abdelkarim Abousalham, Amel Bouanane-Darenfed, Hocine Hacene, Florence Lederer, Laura Baciou, Bassem Jaouadi
Summary: In this study, the sapN gene encoding subtilisin SAPN from Melghiribacillus thermohalophilus Nari2AT was successfully isolated, cloned, and expressed in both P. pastoris and E. coli, with His6-tagged enzymes showing the highest activity and stability. Comparative analysis revealed that these enzymes have great potential for industrial applications due to their catalytic efficiency and hydrolysis degree.
PROCESS BIOCHEMISTRY
(2021)
Article
Chemistry, Applied
Sondes Mechri, Khelifa Bouacem, Taha-Bilel Chalbi, Marwa Khaled, Fawzi Allala, Amel Bouanane-Darenfed, Hocine Hacene, Bassem Jaouadi
Summary: This study focuses on the protease production and characteristics of a strain called TN-X30, identified as Streptomyces mutabilis. The researchers found that optimizing the culture conditions and using lyophilization techniques improved the protease yield and stability. The TN-X30 protease showed excellent performance in detergent formulations, reducing potential environmental chemical threats.
JOURNAL OF SURFACTANTS AND DETERGENTS
(2022)
Article
Ecology
Sidali Kourdali, Kamel Boudjema, Abdellah Meknachi, Nabila Bounakous, Bassem Jaouadi, Sondes Mechri, Abdelmalek Badis
Summary: The present research aims to evaluate the variation of several responses in marine mussels exposed to different sub-lethal concentrations of copper and lead. The results showed that temperature and salinity variations were not significant enough to interfere with the effect of the pollutants. Changes in nitrogen and phosphorus mineral matter were found to be a physiological disturbance in both species. During the contamination cycles, catalase induction and protein depletion reflected the oxidative stress caused by the metals. However, the catalase activity returned to normal state during the decontamination cycles.
REGIONAL STUDIES IN MARINE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ismail Hasnaoui, Ahlem Dab, Sondes Mechri, Houssam Abouloifa, Ennouamane Saalaoui, Bassem Jaouadi, Alexandre Noiriel, Abdeslam Asehraou, Abdelkarim Abousalham
Summary: A novel extracellular lipase P22 from Penicillium crustosum Thom was isolated and characterized. The specific activities of P22 lipase were found to be 5000 and 10,000 U/mg on olive oil and trioctanoin emulsions, respectively. It exhibited clear regioselectivity toward the sn-1 position of surface-coated triglycerides and showed high activity and stability in the presence of water-immiscible organic solvents, surfactants, and oxidizing agents. It also showed compatibility with commercial laundry detergents and effectively removed oil stains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Sondes Hamdi, Fawzi Allala, Sondes Mechri, Khelifa Bouacem, Hatem Rekik, Hocine Hacene, Bassem Jaouadi, Marilize Le Roes-Hill, Nadia Zarai Jaouadi
Summary: Textile dyes in wastewater from textile factories pose a significant environmental problem, threatening aquatic life. In this study, a novel enzyme called MnP AN30 was found to effectively decolorize various textile dyes with high efficiency. The gene encoding this enzyme was cloned and expressed, and the recombinant enzyme showed similar biochemical properties to the native enzyme. This research provides a potential candidate for the biological treatment and decolorization of synthetic textile dyes.
PROCESS BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Sondes Mechri, Fawzi Allala, Khelifa Bouacem, Ismail Hasnaoui, Hassan Gwaithan, Taha Bilel Chalbi, Ennouamane Saalaoui, Abdeslam Asehraou, Alexandre Noiriel, Abdelkarim Abousalham, Hocine Hacene, Amel Bouanane-Darenfed, Marilize Le Roes-Hill, Bassem Jaouadi
Summary: In this study, a serine alkaline protease named SPSM was purified and characterized from Streptomyces mutabilis strain TN-X30. The SPSM gene was cloned and over-expressed in E. coli BL21(DE3)pLysS and E. coli BL21-AITM strains. The immobilization of SPSM using encapsulation and adsorption-encapsulation approaches and different carriers was investigated. The results showed that the immobilized SPSM exhibited similar characteristics to the soluble form, and the white clay and kaolin used as binders showed great potential in the immobilization of SPSM.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biotechnology & Applied Microbiology
Boutheina Bessadok, Bassem Jaouadi, Thomas Brueck, Andrea Santulli, Concetta Maria Messina, Saloua Sadok
Summary: This study isolated and characterized five strains of marine yeast, which showed high protein content, essential amino acids, and polyunsaturated fatty acids. They also exhibited good cell viability and safety. Based on their composition and literature investigation, these yeasts could be classified as oleaginous yeasts, single cell protein (SCP), and single cell oil (SCO) producers, providing alternative ingredients for various sectors including food and feed.
FERMENTATION-BASEL
(2022)
Article
Horticulture
Adiba Benahmed Djilali, Rebiha Mehraz, Khelifa Bouacem, Abdelouahb Benseddik, Idir Moualek, Mohamed Nabiev, Abdelmadjid Benzara
Summary: The study evaluated the phytochemical, antimicrobial, and insecticidal properties of tannins derived from Cydonia oblonga fruit, showing significant extraction levels using different solvents and salts. The extracted tannins had no antimicrobial activity against the tested strains, but demonstrated a bio-insecticidal effect on Tribolium confusum adults. The findings suggest that Cydonia oblonga fruit could be a potential alternative for the development of natural drugs and insecticides against parasites.
INTERNATIONAL JOURNAL OF FRUIT SCIENCE
(2021)
Article
Environmental Sciences
Mouna Ben Elhoul, Nadia Zarai Jaouadi, Khelifa Bouacem, Fawzi Allala, Hatem Rekik, Sondes Mechri, Haifa Khemir Ezzine, Neila Miled, Bassem Jaouadi
Summary: The keratin-degrading bacterium Actinomadura viridilutea DZ50 secretes a keratinase (KERDZ) with potential industrial interest. Recombinant enzyme rKERDZ has been shown to completely degrade chicken feather and remove wool/hair from various hides, while preserving collagen structure. The enzymatic process reduces biological and chemical oxygen demands in leather processing and offers a greener alternative to traditional dehairing methods.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
