期刊
BIORESOURCE TECHNOLOGY
卷 188, 期 -, 页码 49-55出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.01.099
关键词
Volatile fatty acids; Microbial lipids; Biodiesel; Biofuel; Fermentation
资金
- Bill & Melinda Gates Foundation, United States [1019896]
Lipid accumulation in the oleaginous yeast Cryptococcus albidus was evaluated using mixtures of volatile fatty acids (VFA) as substrates. In general, batch growth under nitrogen limitation led to higher lipid accumulation using synthetic VFA. During batch growth, an initial COD:N ratio of 25:1 mg COD:mg N led to maximum intracellular lipid accumulation (28.3 +/- 0.7% g/g dry cell weight), which is the maximum reported for C. albidus using VFA as the carbon source, without compromising growth kinetics. At this feed COD:N ratio, chemostat cultures fed with synthetic VFA yielded statistically similar intracellular lipid content as batch cultures (29.9 +/- 1.9%, g/g). However, batch cultures fed with VFA produced from the fermentation of food waste, yielded a lower lipid content (14.9 +/- 0.1%, g/g). The lipid composition obtained with synthetic and food-waste-derived VFA was similar to commercial biodiesel feedstock. We therefore demonstrate the feasibility of linking biochemical waste treatment and biofuel production using VFA as key intermediates. (C) 2015 Elsevier Ltd. All rights reserved.
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