期刊
BIORESOURCE TECHNOLOGY
卷 198, 期 -, 页码 557-563出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.09.031
关键词
Haematococcus pluvialis; Heterotrophy; Dilution; Photoinduction; Astaxanthin
资金
- National High Technology Research and Development Program of China [2015AA020602]
- National Natural Science Foundation of China [31500062]
- National Key Technologies RD Program [2011BAD23B04]
- National Basic Research Program of China (973 Program) [2011CB200903, 2011CB200904]
- China Postdoctoral Science Foundation [2013M530183, 2014T70400]
- Fundamental Research Funds for the Central Universities [222201414024]
A novel cultivation strategy called Sequential Heterotrophy-Dilution-Photoinduction was successfully applied in the cultivation of Haematococcus pluvialis to produce astaxanthin effectively. Cells were first cultivated heterotrophically to achieve a high cell density, then were diluted to a suitable concentration and switched to a favorable environment for cells acclimation. Finally, the culture was transferred to high light environment for astaxanthin accumulation. By this strategy, the dry cell weight of 26 g/L and biomass productivity of 64.1 mg/L/h were obtained in heterotrophy stage which surpassed ever before reported in literatures. Meanwhile, the cells could accumulate considerable astaxanthin up to 4.6% of dry cell weight after 10 days of photoinduction. Furthermore, the application prospects of the strategy were confirmed further by outdoor experiments. Therefore, this novel strategy provided a promising approach for high-efficient production of natural astaxanthin from H. pluvialis to meet the huge demand of this high value product. (C) 2015 Elsevier Ltd. All rights reserved.
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