期刊
BIORESOURCE TECHNOLOGY
卷 191, 期 -, 页码 362-368出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2015.03.068
关键词
Lipid; Chemical modulators; Metabolomics; C. cohnii
资金
- National High-tech R&D Program (863 program) [2012AA02A707]
- National Basic Research Program of China (973 program) [2014CB745101]
- Doctoral Program of Higher Education of China [20120032110020, 20130032120022]
- Tianjin Municipal Science and Technology Commission
- Zaoneng Biotechnology Inc.
In the study, fourteen chemical modulators from five groups (i.e., auxin, gibberellin, cytokinin, signal transducer and amine) were evaluated for their effects on lipid accumulation in Crypthecodinium cohnii. The results showed that naphthoxyacetic acid (BNOA), 2-chlorodracylicacid, salicylic acid (SA), abscisic acid (ABA) and ethanolamine (ETA), increased lipid accumulation in C. cohnii by 10.00-18.78%. In addition, the combined uses of the above chemicals showed that two combinations, 1.0 mg/L SA & 152.7 mg/L ETA and 4.0 mg/L BNOA & 152.7 mg/L ETA, increased lipid accumulation by 22.45% and 20.54%, respectively. Moreover, a targeted metabolomic approach was employed to decipher the possible mechanisms responsible for the increased lipid accumulation, and the results showed that the enhanced metabolism in glycolysis and TCA cycle as well as the decreased metabolism in PPP pathway could be important for the stimulatory roles of BNOA & ETA and SA & ETA on lipid accumulation in C. cohnii. (C) 2015 Elsevier Ltd. All rights reserved.
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