Article
Cell Biology
Melanie Tingler, Amelie Brugger, Kerstin Feistel, Axel Schweickert
Summary: This study reveals the role of Dmrt2 transcription factor in LRO ciliogenesis and sLRO cell specification in the formation of the vertebrate left-right axis, as well as its functional connection to somitogenesis and LR determination.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Kadeen Forrest, Alexandria C. Barricella, Sonny A. Pohar, Anna Maria Hinman, Jeffrey D. Amack
Summary: Human laterality disorders are caused by embryonic developmental issues. Studying the development of LRO (left-right organizer) in zebrafish models helps us understand the origins of laterality disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Masuelli Sofia, Real Sebastian, Campoy Emanuel, Maria Teresita Branham, Diego Matias Marzese, Salomon Matthew, Gerardo De Blas, Arias Rodolfo, Levin Michael, Roque Maria
Summary: This study discovered differential DNA methylation and bioelectric differences between left-sided and right-sided breast tumors, which were associated with tumor proliferation. These findings provide new targets for future breast cancer therapeutic approaches.
MOLECULAR MEDICINE
(2022)
Article
Multidisciplinary Sciences
Natalia Petri, Rhea Nordbrink, Nikoloz Tsikolia, Stanislav Kremnyov
Summary: Previously, it was believed that there is an early mechanism based on cytoskeletal processes in Xenopus embryos during the early cleavage stages, supported by evidence from chemical inhibition of cytoskeletal protein. However, further analysis revealed that the reported abnormalities only occur after treatment at gastrula-neurula stages, just prior to and during the operation of the left-right organizer. Therefore, these left-right abnormalities cannot be considered as evidence for the early symmetry breaking event in Xenopus embryos.
Article
Multidisciplinary Sciences
Helen M. M. Bellchambers, Amruta R. R. Phatak, Mardi J. J. Nenni, Maria B. B. Padua, Hongyu Gao, Yunlong Liu, Stephanie M. M. Ware
Summary: In this study, the transcriptome of the LRO in mice was defined using single cell RNA sequencing and fluorescent activated cell sorting. The analysis revealed genes associated with cilia and laterality, as well as 127 novel LRO genes. This resource will be valuable for future studies on LRO morphogenesis, laterality establishment, and genetic causes of heterotaxy.
SCIENTIFIC REPORTS
(2023)
Article
Developmental Biology
Christopher J. Derrick, Adrian Santos-Ledo, Lorraine Eley, Isabela Andhika Paramita, Deborah J. Henderson, Bill Chaudhry
Summary: The establishment of left-right axis is crucial for the placement, morphogenesis, and function of internal organs. Cilia-driven fluid flow in the embryonic node is proposed to be dependent on left-right specification. The JNK gene family plays an important role in the development and function of the zebrafish node, determining the length of nodal cilia, generating flow in the node, and restricting southpaw to the left lateral plate mesoderm.
Review
Cell Biology
Rosie B. Little, Dominic P. Norris
Summary: The initial breaking of left-right symmetry in the embryo is controlled by motile cilia-driven leftward fluid flow in the left-right organiser (LRO), resulting in L-R asymmetric gene expression. The activation of the Nodal-Pitx2 pathway is only on the left side in more lateral tissues. The events in the Lateral Plate Mesoderm (LPM) are conserved through the vertebrate lineage.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
Leah T. Johnstone, Emma M. Karlsson, David P. Carey
Summary: Neuroscientific techniques show that left-handers have more unusual cerebral asymmetries for language compared to right-handers, with left-handers demonstrating less lateralization for language, faces, and bodies when they have typical cerebral asymmetries.
Article
Biochemistry & Molecular Biology
Jianguo Wang, Sidi Ma, Peijie Yu, Xionglei He
Summary: The human brain exhibits anatomical symmetry, but also possesses fine-scale structural asymmetries that contribute to distinct functional divisions. Previous studies suggested that these asymmetric structures are primate specific or unique to humans, indicating recent evolution of genes responsible for human brain asymmetry. In our study, we identified over 1,500 traits associated with human brain asymmetry and discovered approximately 200 genes that are predominantly upregulated in brain tissues. Surprisingly, these genes are evolutionarily ancient and show brain-specific upregulation in humans, indicating the role of ancient genes in shaping the structural asymmetry of the human brain over time.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Article
Cell Biology
Yosuke Tanaka, Ai Morozumi, Nobutaka Hirokawa
Summary: Left-dominant [Ca2+]i elevation at the left margin of the ventral node plays a crucial role in determining the initial laterality of mouse embryos. This process is dependent on extracellular leftward fluid flow, fibroblast growth factor receptor (FGFR)/ sonic hedgehog (Shh) signaling, and the PKD1L1 polycystin subunit, although their interrelationships are not fully understood. By studying KikGR-PKD1L1 knockin mice, researchers have discovered that leftward nodal flow guides PKD1L1-containing fibrous strands and facilitates Nodal mediated [Ca2+]i elevation at the left margin. They propose that the leftward transfer of polycystin-containing fibrous strands determines the left-right asymmetry in developing embryos.
DEVELOPMENTAL CELL
(2023)
Article
Cell Biology
Lu Yan, Hailing Yin, Yiwei Mi, Yu Wu, Yufang Zheng
Summary: Cilia are specialized organelles involved in signal transduction and embryonic morphogenesis. Dysfunction of cilia can lead to developmental defects, including neural tube defects (NTDs). In this study, mouse models with disrupted expression of Wdr60 and Wdr34 were established using piggyBac transposon. Wdr60 homozygote mice exhibited head malformation and downregulated Sonic Hedgehog signaling, while Wdr34 homozygote mice showed open cranial and caudal neural tube defects. Both WDR60 and WDR34 interact with IFT88, but only WDR34 interacts with IFT140, indicating their distinct functions in modulating neural tube development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Xin-Xin Fu, Ding-Hao Zhuo, Ying-Jie Zhang, Yun-Fei Li, Xiang Liu, Yan-Yi Xing, Ying Huang, Yi-Fan Wang, Tao Cheng, Dan Wang, Si-Han Chen, Yi-Jian Chen, Guan-Nan Jiang, Fu- Lu, Yu Feng, Xiao Huang, Jun Ma, Wei Liu, Ge Bai, Peng-Fei Xu
Summary: The emergence of left-right (LR) asymmetry in a patterning field along the anterior-posterior axis is still an unresolved problem in developmental biology. This study identifies Follistatin (Fst) as a regulator that restricts the propagation of Nodal ortholog Southpaw (Spaw) in the right lateral plate mesoderm (LPM) and facilitates the establishment of a robust LR asymmetric Nodal patterning. Furthermore, Fst inhibits the Activin-Nodal signaling pathway in the forebrain, contributing to the orderly propagation of asymmetric Nodal activation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Psychology, Biological
Zhiqiang Sha, Dick Schijven, Amaia Carrion-Castillo, Marc Joliot, Bernard Mazoyer, Simon E. Fisher, Fabrice Crivello, Clyde Francks
Summary: Genes associated with brain asymmetry are linked to microtubule-related genes and embryonic brain expression, and have overlap with genetic variants related to autism, educational attainment, and schizophrenia. Left-right hemispheric asymmetry is crucial for healthy brain organization and language functions, but the mechanism behind establishing the human brain's left-right axis remains unidentified.
NATURE HUMAN BEHAVIOUR
(2021)
Article
Dentistry, Oral Surgery & Medicine
Jana Filusova, Iveta Putnova, Pavel Hurnik, Zdenek Danek, Ctirad Machacek, Jan Stembirek, Marcela Buchtova, Barbora Moldovan Putnova
Summary: This study investigated alterations in the occurrence of primary cilia and associated signaling in ameloblastoma, a common odontogenic tumor. The study found differences in the distribution and appearance of primary cilia among histological subtypes of ameloblastoma. The expression of molecules downstream of the SHH signaling pathway was upregulated, and inhibition of the pathway led to downregulation of SHH effector genes and cell cycle regulators. These findings suggest that SHH inhibitors could be a potential therapeutic target for treating ameloblastoma.
ARCHIVES OF ORAL BIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Takehisa Kudo, Maiko Kawasaki, Katsushige Kawasaki, Fumiya Meguro, Jun Nihara, Izumi Honda, Madoka Kitamura, Akira Fujita, Kazuaki Osawa, Kaya Ichikawa, Takahiro Nagai, Yoko Ishida, Paul T. Sharpe, Takeyasu Maeda, Isao Saito, Atsushi Ohazama
Summary: The present study suggests that the function of primary cilia, mediated by Ift88, is critical for the secretory stage of amelogenesis, involving the Shh signaling pathway.
Article
Genetics & Heredity
Natalia A. Shylo, Kasey J. Christopher, Alejandro Iglesias, Aaron Daluiski, Scott D. Weatherbee
Editorial Material
Cell Biology
Natalia A. Shylo, Scott D. Weatherbee
DEVELOPMENTAL CELL
(2016)
Article
Dentistry, Oral Surgery & Medicine
P. Cela, M. Hampl, N. A. Shylo, K. J. Christopher, M. Kavkova, M. Landova, T. Zikmund, S. D. Weatherbee, J. Kaiser, M. Buchtova
JOURNAL OF DENTAL RESEARCH
(2018)
Article
Developmental Biology
Stephanie Vadasz, Jonathan Marquez, Maria Tulloch, Natalia A. Shylo, Martin I. Garcia-Castro
Article
Biochemistry & Molecular Biology
Diane E. Peters, Roman Szabo, Stine Friis, Natalia A. Shylo, Katiuchia Uzzun Sales, Kenn Holmbeck, Thomas H. Bugge
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Multidisciplinary Sciences
Kristin E. Murphy, Natalia A. Shylo, Katherine A. Alexander, Angela J. Churchill, Cecilia Copperman, Maria J. Garcia-Garcia
Article
Cell & Tissue Engineering
Yaochen Li, Matthew Aaron Hibbs, Ashley Lauren Gard, Natalia Aliakseeuna Shylo, Kyuson Yun
Article
Genetics & Heredity
Roman Szabo, Katiuchia Uzzun Sales, Peter Kosa, Natalia A. Shylo, Sine Godiksen, Karina K. Hansen, Stine Friis, J. Silvio Gutkind, Lotte K. Vogel, Edith Hummler, Eric Camerer, Thomas H. Bugge
Article
Anatomy & Morphology
Raul E. Diaz, Natalia A. Shylo, Daniela Roellig, Marianne Bronner, Paul A. Trainor
DEVELOPMENTAL DYNAMICS
(2019)
Article
Multidisciplinary Sciences
Yi-Cheng Chang, Jan Manent, Jan Schroeder, Siew Fen Lisa Wong, Gabriel M. Hauswirth, Natalia A. Shylo, Emma L. Moore, Annita Achilleos, Victoria Garside, Jose M. Polo, Paul Trainor, Edwina McGlinn
Summary: This study identifies Nr6a1 as a master regulator of trunk development in mice, controlling vertebral number and segmentation of the trunk region autonomously from other axial regions. Nr6a1 is also essential for the timely progression of Hox signatures and cell fate choice within axial progenitors.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Natalia A. Shylo, Sarah E. Smith, Andrew J. Price, Fengli Guo, Melainia McClain, Paul A. Trainor
Summary: Using live imaging, veiled chameleon embryos were observed to undergo asymmetric morphological changes that trigger asymmetric expression of Nodal cascade in the left lateral plate mesoderm. This makes veiled chameleons a new and unique model for studying the evolution of L-R patterning.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)