期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 104, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2019.103558
关键词
Bacterial agglutination; GalBL; Japanese sea bass; Sugar binding; Survival rate
资金
- Program for the National Natural Science Foundation of China [31802334, 31772876]
- Natural Science Foundation of Zhejiang Province [LZ18C190001]
- China Postdoctoral Science Foundation [2018M630648]
- Natural Science Foundation of Ningbo City of China [2018A610346]
- Scientific Innovation Team Project of Ningbo [2015C110018]
- K.C. Wong Magna Fund in Ningbo University
Mannose-binding lectin (MBL) plays an important role in host immune responses against pathogens. LjMBL-like-1 was identified from Japanese sea bass (Lateolabrax japonicas), which has selectivity for galactose. Herein, this lectin might be better designated as galactose-binding lectin (LjGalBL-1). LjGalBL-1 transcripts were detected in all tested tissues, with highest expression in liver. Upon Vibrio harveyi infection, LjGalBL-1 mRNA expression was increased in major immune-related tissues, and protein levels in serum were also upregulated. Recombinant. LjGalBL-1 (rLjGalBL-1) bound to monosaccharides and polysaccharides, and both rLjGalBL-1 and native LjGalBL1 (nLjGaIBL-1) agglutinated three Gram-positive bacteria (Staphylococcus aureus, Streptococcus iniae and Micrococcus luteus) and four Gram-negative bacteria (Aeromonas hydrophila, Edwardsiella tarda, Vibrio anguillarum and V. harveyi) in a Ca2+-dependent manner in vitro. Moreover, rLjGalBL-1 increased the survival rate of V. harveyi-infected fish and decreased bacterial load in liver, spleen, kidney and blood. Thus, LjGalBL-1 protects L. japonicas against V. harveyi infection.
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