Review
Immunology
Hideaki Fujiwara
Summary: This article discusses the impact of allo-HSCT on intestinal microbiota and metabolites, as well as the mechanisms for regulating experimental GVHD through dietary intervention or metabolites produced by intestinal bacteria post-allo-HSCT. It also summarizes the major findings of microbiome-based immunotherapeutic approaches for tissue protection against experimental GVHD. Understanding the complex relationships within the gut microbiota-metabolites-host tissues axis is crucial for insight into GVHD pathogenesis and the development of new therapeutic strategies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Zhenyang Gu, Qian Xiong, Lu Wang, Lili Wang, Fei Li, Cheng Hou, Liping Dou, Baoli Zhu, Daihong Liu
Summary: This study indicates that gut dysbiosis in platforms of ATG-based myeloablative allo-HCT features loss of bacterial diversity. The diversity of the intestinal flora at the engraftment period is an independent predictor of longer survival.
Article
Hematology
Lijie Han, Haiyan Zhang, Ping Ma, Jie Peng, Yilu Li, Jiaying Wu, Yuanyuan Li, Jifeng Yu, Wei Li, Mengmeng Zhang, Jia Bao He, Zhiping Fan, Weimin Wang, Li'na Sang, Hui Sun, Qifa Liu, Yang Liu, Zhongxing Jiang
Summary: The intestinal microbiota score at neutrophil recovery could predict survival following allo-HSCT. A low score was associated with better 3-year cumulative overall survival and lower mortality. High score was found to be an independent risk factor for poor survival.
ANNALS OF HEMATOLOGY
(2022)
Article
Immunology
Andrew J. Innes, Benjamin H. Mullish, Rohma Ghani, Richard M. Szydlo, Jane F. Apperley, Eduardo Olavarria, Renuka Palanicawandar, Edward J. Kanfer, Dragana Milojkovic, Julie A. K. McDonald, Eimear T. Brannigan, Mark R. Thursz, Horace R. T. Williams, Frances J. Davies, Julian R. Marchesi, Jiri Pavlu
Summary: The study showed that fecal microbiota transplantation (FMT) from healthy donors for MDRO-colonized patients can lead to higher survival rates, reduce the need for intensive care and fever occurrence, and potentially ameliorate negative clinical outcomes associated with MDRO colonization.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Health Care Sciences & Services
Andrea Pession, Daniele Zama, Edoardo Muratore, Davide Leardini, Davide Gori, Federica Guaraldi, Arcangelo Prete, Silvia Turroni, Patrizia Brigidi, Riccardo Masetti
Summary: FMT has been used in allo-HSCT patients for therapeutic and preventive purposes, but further research on the biological mechanisms is needed. Concerns exist regarding the safety and efficacy of administering microbial consortia in immune-compromised patients.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Hematology
Francesca Marzuttini, Antonella Mancusi, Samanta Bonato, Mario Griselli, Sara Tricarico, Genni Casarola, Matteo Paradiso, Loredana Ruggeri, Adelmo Terenzi, Mara Merluzzi, Anna Prigitano, Anna Maria Tortorano, Lucia Pitzurra, Brunangelo Falini, Alessandra Carotti, Andrea Velardi, Antonio Pierini
Summary: The study evaluated the safety and outcomes of using rifaximin prophylaxis in patients undergoing hematopoietic stem cell transplantation, finding an increased risk of Candida spp. infections in rifaximin-treated patients compared to historical controls, highlighting the need for larger prospective studies to assess the overall impact of rifaximin prophylaxis on patient outcomes.
ANNALS OF HEMATOLOGY
(2021)
Article
Immunology
Armin Rashidi, Jonathan U. Peled, Maryam Ebadi, Tauseef Ur Rehman, Heba Elhusseini, LeeAnn T. Marcello, Hossam Halaweish, Thomas Kaiser, Shernan G. Holtan, Alexander Khoruts, Daniel J. Weisdorf, Christopher Staley
Summary: This study found that the gut microbiota Blautia can prevent neutropenic fever by improving intestinal health. Restoring Blautia may help reduce the incidence of neutropenic fever and related complications.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Oncology
Zev M. Nakamura, Allison M. Deal, Donald L. Rosenstein, Laura J. Quillen, Stephanie A. Chien, William A. Wood, Thomas C. Shea, Eliza M. Park
Summary: The study found that over 50% of cancer patients undergoing allogeneic transplantation exhibit cognitive impairment prior to the procedure. Factors related to worse cognitive function include hematopoietic cell transplantation-comorbidity index score and history of alcohol or substance abuse. Pre-transplant evaluation of medical comorbidities and substance abuse history may help identify patients at risk for cognitive impairment.
SUPPORTIVE CARE IN CANCER
(2021)
Article
Hematology
Akihisa Hino, Kentaro Fukushima, Shinsuke Kusakabe, Tomoaki Ueda, Takao Sudo, Jiro Fujita, Daisuke Motooka, Aya K. K. Takeda, Natsuko O. O. Shinozaki, Satoshi Watanabe, Takafumi Yokota, Hirohiko Shibayama, Shota Nakamura, Naoki Hosen
Summary: This study demonstrates the persistence of gut microbial alterations in long-term survivors after allo-HSCT and their association with the development and exacerbation of late complications.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Article
Oncology
Siddika Songul Yalcin, Tekin Aksu, Baris Kuskonmaz, Namik Yasar Ozbek, Vicente Perez-Brocal, Melda Celik, Duygu Uckan Cetinkaya, Andres Moya, Ener Cagri Dinleyici
Summary: This study evaluated the mycobiota composition pre- and post-HSCT in children with thalassemia, revealing a decrease in diversity following transplantation. Particularly, there was a predominance of Malassezia in children with GVHD. Further research is needed to understand this phenomenon in children with GVHD.
PEDIATRIC BLOOD & CANCER
(2022)
Review
Nutrition & Dietetics
Edoardo Muratore, Davide Leardini, Francesco Baccelli, Francesco Venturelli, Arcangelo Prete, Riccardo Masetti
Summary: Allogeneic hematopoietic stem cell transplantation is a potentially curative strategy, but it is associated with complications and mortality. The disruption of gut microbiota has been linked to these complications, and nutritional strategies are being considered as effective methods of modulating the gut microbiota and improving clinical outcomes.
FRONTIERS IN NUTRITION
(2022)
Article
Hematology
Yoshimitsu Shimomura, Sho Komukai, Tetsuhisa Kitamura, Takayoshi Tachibana, Shuhei Kurosawa, Hidehiro Itonaga, Shokichi Tsukamoto, Noriko Doki, Yuta Katayama, Ayumu Ito, Masashi Sawa, Yasunori Ueda, Hirohisa Nakamae, Yuichiro Nawa, Masatsugu Tanaka, Yasuyuki Arai, Shuichi Ota, Keisuke Kataoka, Tetsuya Nishida, Junya Kanda, Takahiro Fukuda, Yoshiko Atsuta, Ken Ishiyama
Summary: This study aimed to identify risk factors for patients with CK-MDS undergoing allogeneic HCT. The results showed that older age, male sex, poor comorbidity index, transfusion requirements, incomplete remission, high number of karyotype abnormalities, and monosomal karyotype were significantly associated with overall survival in CK-MDS patients.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Article
Immunology
Alessandro Busca, Natascia Cinatti, Jessica Gill, Roberto Passera, Chiara Maria Dellacasa, Luisa Giaccone, Irene Dogliotti, Sara Manetta, Silvia Corcione, Francesco Giuseppe De Rosa
Summary: This study aimed to assess the epidemiology and prognostic factors of invasive fungal infections (IFI) in a large cohort of allo-HSCT patients. The results showed that the presence of IFI had a significant impact on overall survival at 1 year, and donor type was significantly associated with the risk of IFI.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Hematology
Oriana Miltiadous, Nicholas R. Waters, Hana Andrlova, Anqi Dai, Chi L. Nguyen, Marina Burgos da Silva, Sarah Lindner, John Slingerland, Paul Giardina, Annelie Clurman, Gabriel K. Armijo, Antonio L. C. Gomes, Madhavi Lakkaraja, Peter Maslak, Michael Scordo, Roni Shouval, Anna Staffas, Richard O'Reilly, Ying Taur, Susan Prockop, Jaap Jan Boelens, Sergio Giralt, Miguel-Angel Perales, Sean M. Devlin, Jonathan U. Peled, Kate A. Markey, Marcel R. M. van den Brink
Summary: This study analyzed stool samples and peripheral blood samples from patients undergoing allogeneic hematopoietic cell transplantation, and found that the diversity of intestinal microbiota and the relative abundance of specific bacterial genera were associated with the recovery of immune cells.
Review
Immunology
Fatina Siwczak, Elise Loffet, Mathilda Kaminska, Hristina Koceva, Maxime M. Mahe, Alexander S. Mosig
Summary: The article discusses the anatomical structure and biological functions of the gut, introduces the method of using organ-on-chip technology for research, and discusses the latest developments and future research directions on intestinal stem cell differentiation, tissue formation, and the interaction of the gut with the microbiota.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Katie L. O'Brien, Nadine Assmann, Eimear O'Connor, Cathal Keane, Jessica Walls, Chloe Choi, Peter J. Oefner, Clair M. Gardiner, Katja Dettmer, David K. Finlay
Summary: Cellular metabolism plays a crucial role in regulating NK cell responses, with SREBP and cMyc controlling metabolic and functional responses. Polyamine synthesis is essential for NK cell glycolytic and oxidative metabolism, while spermidine supports protein translation. Inhibition of hypusination impacts NK cell metabolism and effector functions.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Critical Care Medicine
Selena Z. Kuo, Katja Dettmer, Medini K. Annavajhala, David H. Chong, Anne-Catrin Uhlemann, Julian A. Abrams, Peter J. Oefner, Daniel E. Freedberg
Summary: The study investigated the relationship between urinary 3-IS levels and patient outcomes in the ICU, finding that low 3-IS levels were associated with fewer ICU-free days and increased one-year mortality. Further research should explore urinary 3-IS as an ICU biomarker.
JOURNAL OF CRITICAL CARE
(2021)
Article
Multidisciplinary Sciences
Jennifer K. Dowling, Remsha Afzal, Linden J. Gearing, Mariana P. Cervantes-Silva, Stephanie Annett, Gavin M. Davis, Chiara De Santi, Nadine Assmann, Katja Dettmer, Daniel J. Gough, Glenn R. Bantug, Fidinny Hamid, Frances K. Nally, Conor P. Duffy, Aoife L. Gorman, Alex M. Liddicoat, Ed C. Lavelle, Christoph Hess, Peter J. Oefner, David K. Finlay, Gavin P. Davey, Tracy Robson, Annie M. Curtis, Paul J. Hertzog, Bryan R. G. Williams, Claire E. McCoy
Summary: The study reveals that Arg2 is a protein in inflammatory macrophages regulated by miR-155 and IL-10, crucial for IL-10-induced modulation of mitochondrial dynamics and oxidative respiration. Arg2 mediates this process by increasing the activity of complex II and is essential for IL-10-mediated downregulation of inflammatory mediators.
NATURE COMMUNICATIONS
(2021)
Article
Allergy
Anders O. Petersen, Martta Jokinen, Damian R. Plichta, Gerhard Liebisch, Wolfram Gronwald, Katja Dettmer, Peter J. Oefner, Hera Vlamakis, Daniel C. Chung, Annamari Ranki, Ramnik J. Xavier
Summary: The gut microbiome in APS-1 patients is correlated with APS-1 symptoms, and probiotic intervention can alleviate some gastrointestinal symptoms. Furthermore, autoantibodies against cytokines appear to shape the gut microbiome by positively correlating with a taxonomically consistent group of bacteria.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Oncology
Raffaela S. Berger, Christian J. Wachsmuth, Magdalena C. Waldhier, Kathrin Renner-Sattler, Simone Thomas, Anuhar Chaturvedi, Hans-Helmut Niller, Elisabeth Bumes, Peter Hau, Martin Proescholdt, Wolfram Gronwald, Michael Heuser, Marina Kreutz, Peter J. Oefner, Katja Dettmer
Summary: Somatic mutations in isocitrate dehydrogenase lead to excessive production of D-2-hydroxyglutarate in certain malignancies. A related metabolite, 2-hydroxyglutarate-gamma-lactone, was discovered and it may impact the anti-tumor immune response. The formation of the lactone is likely linked to transport processes across the cell membrane and favored at low environmental pH.
Article
Biochemistry & Molecular Biology
Stephanie Arndt, Fadi Fadil, Katja Dettmer, Petra Unger, Marko Boskovic, Claudia Samol, Anja-Katrin Bosserhoff, Julia L. Zimmermann, Michael Gruber, Wolfram Gronwald, Sigrid Karrer
Summary: Cold Atmospheric Plasma (CAP) has anti-tumor effects transmitted through direct treatment or plasma-treated solutions (PTS) containing various amino acids and vitamins. Different plasma modes and treatment durations affect the production of reactive species in PTS, influencing the composition of active components and anti-tumor mechanisms. The findings are crucial for plasma device development and tumor treatment effectiveness.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Eva Scholler, James Marks, Virginie Marchand, Astrid Bruckmann, Christopher A. Powell, Markus Reichold, Christian Daniel Mutti, Katja Dettmer, Regina Feederle, Stefan Huttelmaier, Mark Helm, Peter Oefner, Michal Minczuk, Yuri Motorin, Markus Hafner, Gunter Meister
Summary: The mitochondrial protein METTL8 plays a crucial role in facilitating m(3)C methylation on mt-tRNAs, impacting respiratory chain activity and pancreatic cancer development. This modification leads to optimal composition and function of the mitochondria respiratory chain, with implications for patient survival and cellular metabolism.
Article
Immunology
Adam Wahida, Madeleine Mueller, Andreas Hiergeist, Bastian Popper, Katja Steiger, Caterina Branca, Markus Tschurtschenthaler, Thomas Engleitner, Sainitin Donakonda, Jordy De Coninck, Rupert Oellinger, Marie K. Pfautsch, Nicole Mueller, Miguel Silva, Sinem Usluer, Erik Thiele Orberg, Jan P. Boettcher, Nicole Pfarr, Martina Anton, Julia B. Slotta-Huspenina, Andreas G. Nerlich, Tobias Madl, Marijana Basic, Andre Bleich, Geert Berx, Jurgen Ruland, Percy A. Knolle, Roland Rad, Timon E. Adolph, Peter Vandenabeele, Hirokazu Kanegane, Andre Gessner, Philipp J. Jost, Monica Yabal
Summary: Deficiency in XIAP leads to severe and therapy-refractory inflammatory bowel disease in XLP2 patients. The combined activity of TLR5 and TNFR2 signaling plays a key role in promoting intestinal inflammation, with TNF acting as a crucial mediator of this process.
SCIENCE IMMUNOLOGY
(2021)
Article
Medicine, General & Internal
Frank Staemmler, Marcello Grassi, Jeffrey W. Meeusen, John C. Lieske, Surendra Dasari, Laurence Dubourg, Sandrine Lemoine, Jochen Ehrich, Eric Schiffer
Summary: The study optimized an eGFR equation targeting the adult population using a nuclear magnetic resonance spectroscopy (NMR) derived 'metabolite constellation' (GFR(NMR)), which showed lower bias and higher accuracy compared to existing eGFR equations. The new GFR(NMR) equation included serum myo-inositol, valine, creatinine, cystatin C, age and sex, providing superior accuracy in assessing eGFR in adult patients with and without chronic kidney disease (CKD).
Article
Nutrition & Dietetics
Conny Stumptner, Vanessa Stadlbauer, Dominic O'Neil, Andre Gessner, Andreas Hiergeist, Kurt Zatloukal, Peter M. Abuja
Summary: This article introduces the European pre-analytical standard for microbiome DNA analysis in human specimens and discusses the importance and advantages of standardization in biomedical research.
Article
Medicine, General & Internal
Markus Fuhrmann, Amauri Schwaeble Santamaria, Renee Scott, Jeffrey W. Meeusen, Marianna Fernandes, John Venz, Victoria Rothe, Frank Staemmler, Jochen Ehrich, Eric Schiffer
Summary: Accurate and precise monitoring of kidney function is crucial for diagnosing chronic kidney disease promptly and reliably. A new eGFR equation called GFR(NMR) has been developed based on nuclear magnetic resonance measurement of serum myo-inositol, valine, and creatinine, along with the immunoturbidometric quantification of serum cystatin C, age, and sex. The analytical performance evaluation of GFR(NMR) according to established guidelines shows good within-laboratory and between-site reproducibility, as well as stability in sample storage. However, there is limited substance interference, which could affect the accuracy of GFR(NMR) results for certain substances.
Review
Infectious Diseases
Bernhard Nausch, Claudia B. Bittner, Martina Hoeller, Dimitri Abramov-Sommariva, Andreas Hiergeist, Andre Gessner
Summary: Epithelial surfaces in humans harbor microbiota that play a crucial role in the host's health and defense against pathogens. Overuse of antibiotics can negatively impact the microbiota, making herbal treatments a more suitable option for uncomplicated infections.
Article
Multidisciplinary Sciences
Andreas Hiergeist, Jean Ruelle, Stefan Emler, Andre Gessner
Summary: The use of NGS-based testing of the bacterial microbiota often leads to inconsistent and non-reproducible results due to variations in analysis pipelines and reference databases. Through comparing five frequently used software packages, we identified that the inconsistencies were caused by the pipelines or the reference databases used. Based on these findings, we recommend certain standards to improve the consistency and reproducibility of microbiome testing in clinical practice.
Article
Nutrition & Dietetics
Hannes Hoelz, Jeannine Heetmeyer, Anastasia Tsakmaklis, Andreas Hiergeist, Kolja Siebert, Federica De Zen, Deborah Haecker, Amira Metwaly, Klaus Neuhaus, Andre Gessner, Maria J. G. T. Vehreschild, Dirk Haller, Tobias Schwerd
Summary: Background: Exclusive enteral nutrition (EEN) is effective for pediatric Crohn's disease (CD) but relapse rates are high after return to regular diet. This study explored the use of autologous fecal microbiota transfer (FMT) for maintaining the benefits of EEN. Methods: Fecal material from pediatric CD patients was turned into FMT capsules, with microbial composition analyzed before and after processing. Results: FMT capsules were successfully generated in most deliveries, but there was variability in stool weight and consistency. Microbial composition was not significantly altered by processing, but samples often had low microbial richness and positive results for potential pathogens or drug resistance genes. Conclusions: Autologous capsule-FMT may not be suitable for maintenance therapy in pediatric CD patients due to high pathogen burden, low microbial diversity, and practical limitations of EEN-conditioned fecal material.
Article
Cardiac & Cardiovascular Systems
Jing Li, Tamara Ruegamer, Christoph Brochhausen, Karin Menhart, Andreas Hiergeist, Lukas Kraemer, Dirk Hellwig, Lars S. Maier, Christof Schmid, Jonathan Jantsch, Christian Schach
Summary: This study investigated diagnostic tools for infective endocarditis and their correlation with mortality outcomes. Positive blood cultures and transesophageal echocardiography were found to be the most sensitive for diagnosing IE, while vegetation size showed high predictive power. Systemic embolism and NT-proBNP levels were associated with mortality.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2022)
