期刊
BIORESOURCE TECHNOLOGY
卷 180, 期 -, 页码 119-127出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2014.12.091
关键词
Wheat bran; Fumaric acid; Rhizopus oryzae; Metabolic profiling; Xylose
资金
- National 863 Project of China [2011AA02A206]
- Key Program of National Natural Science Foundation of China [21236005]
- National 973 Project of China [2013CB733600]
- Program of Introducing Talents of Discipline to Universities [B06006]
In this work, wheat bran (WB) was utilized as feedstock to synthesize fumaric acid by Rhizopus oryzae. Firstly, the pretreatment process of WB by dilute sulfuric acid hydrolysis undertaken at 100 degrees C for 30 min offered the best performance for fumaric acid production. Subsequently, through optimizing the seed culture medium, a suitable morphology (0.55 mm pellets diameter) of R. oryzae was obtained. Furthermore, a metabolic-based approach was developed to profile the differences of intracellular metabolites concentration of R. oryzae between xylose (the abundant sugar in wheat bran hydrolysate (WBH)) and glucose metabolism. The xylitol, sedoheptulose 7-phosphate, ribulose 5-phosphate, glucose 6-phosphate, proline and serine were responsible for fumaric acid biosynthesis limitation in xylose fermentation. Consequently, regulation strategies were proposed, leading to a 149% increase in titer (up to 15.4 g/L). Finally, by combinatorial regulation strategies the highest production was 20.2 g/L from WBH, 477% higher than that of initial medium. (C) 2014 Elsevier Ltd. All rights reserved.
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