Article
Entomology
Cecilia Decker-Franco, Catalina B. Taibo, Julio A. Di Rienzo, Victoria Alfonso, Joel D. Arneodo
Summary: The study compared the pathogenicity of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Rachiplusia nu nucleopolyhedrovirus (RanuNPV) to Rachiplusia nu larvae, finding that the generalist AcMNPV had a faster speed of kill compared to the specific RanuNPV. Co-inoculation resulted in mostly AcMNPV-infected cells, while in sequential inoculations, the first virus administered predominated over the second one. The implications on baculovirus interactions and biocontrol potential were discussed.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiao Li, Fen Wang, Yanyan Xu, Guijun Liu, Caihong Dong
Summary: Hydrophobins are small secreted proteins exclusively found in fungi, and a genome-wide analysis of the hydrophobin family in Cordyceps militaris revealed a greater diversity among class II members and limited gene duplication. Different transcript patterns of four hydrophobin-encoding genes during the life cycle suggest potential varying functions. The discovery of multi-domain hydrophobins in C. militaris could provide valuable insights for further research on hydrophobins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Entomology
Clerison R. Perini, Veronica I. Sosa, Vicente E. Koda, Horacio Silva, Andres A. Risso, Wanessa N. F. Vasconcelos, Carolina F. Goncalves, Gustavo A. Ugalde, Dayanna N. Machado, Caroline B. Bevilacqua, Daniel M. P. Ardisson-Araujo, Kevin Maebe, Guy Smagghe, Ivair Valmorbida, Jerson C. Guedes
Summary: The Plusiinae subfamily has many polyphagous species, with representatives like Chrysodeixis includens and Rachiplusia nu mainly found in crops like soybeans and cotton in South America. Population genetics studies showed diversity differences between the two species, with R. nu populations being more stable and diverse. Haplotype sharing between populations in Argentina and Uruguay suggested potential haplotype exchange, while demographic analysis indicated recent population expansion in C. includens across the American continent.
AGRICULTURAL AND FOREST ENTOMOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nathania Puspitasari, Cheng-Kang Lee
Summary: Hydrophobins are highly surface-active proteins that can reverse surface hydrophobicity through self-assembly at interfaces, with potential applications in surface functional modifications. By fusing cellulose-binding domain with hydrophobins, soluble expression in E. coli can be enhanced for rapid isolation and purification. This strategy also retains the interfacial activity of hydrophobins and accelerates PETase hydrolysis against PET fiber.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Sang-Oh Ahn, Ho-Dong Lim, Sung-Hwan You, Dae-Eun Cheong, Geun-Joong Kim
Summary: Hydrophobins are small proteins secreted by fungi with amphipathic structure, useful for industrial applications. A tagging strategy and modified separation technique have enabled successful soluble expression and efficient purification of recombinant hydrophobin DewA. This approach sheds light on the industrial production of hydrophobins in prokaryotic hosts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Te-Sheng Lien, Der-Shan Sun, Shih-Che Hung, Wen-Sheng Wu, Hsin-Hou Chang
Summary: The study shows that the dengue virus envelope protein domain III can induce neutrophil NETosis processes, and inhibition of Nlrp3 inflammasome can suppress this process, providing a new molecular target for treating dengue-induced inflammatory pathogenesis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Te-Sheng Lien, Der-Shan Sun, Cheng-Yeu Wu, Hsin-Hou Chang
Summary: The study found that endothelial cell death plays an important role in dengue hemorrhagic fever, and this death can be attenuated by inhibiting the Nlrp3 inflammasome. The results suggest that the Nlrp3 inflammasome may be a potential therapeutic target for treating DHF.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Te-Sheng Lien, Hao Chan, Der-Shan Sun, Jhen-Cheng Wu, You-Yen Lin, Guan-Ling Lin, Hsin-Hou Chang
Summary: The study suggests that EIII could be considered as a virulence factor of DENV, and that Nlrp3 inflammasome is a feasible target for developing therapeutic approaches against dengue-induced platelet defects. Blockages of EIII-induced platelet signaling using inhibitors markedly ameliorated DENV- and EIII-induced thrombocytopenia, platelet activation, and cell death.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Te-Sheng Lien, Der-Shan Sun, Wen-Sheng Wu, Hsin-Hou Chang
Summary: This study aimed to investigate the cytotoxicity of EIII-coated silica nanoparticles (EIII-SNPs) on endothelial cells and their potential role in hemorrhage pathogenesis. The results showed that EIII-SNPs induced greater endothelial cytotoxicity in vitro than EIII or silica nanoparticles alone. Moreover, the combination of EIII-SNPs and antiplatelet antibodies resulted in more severe hemorrhage pathogenesis in mice compared to single treatments. These findings suggest that EIII-coated nanoparticles could be used to develop a dengue hemorrhage pathogenesis model and highlight the potential role of EIII in DHF pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Kum-Kang So, Jeesun Chun, Nguyen Ngoc Luong, Hee-Won Seo, Dae-Hyuk Kim
Summary: The study demonstrated the efficient production of various immunocomplex structures of the target protein in Saccharomyces cerevisiae for future development of oral and injectable vaccines against pathogens.
BIOTECHNOLOGY LETTERS
(2021)
Article
Immunology
Hong-Jyun Huang, Martyr Yang, Hsin-Wei Chen, Shuying Wang, Chih-Peng Chang, Tzong-Shiann Ho, Yu-San Kao, Sen-Mao Tien, Hsing-Han Lin, Po-Chun Chang, Yen-Chung Lai, Yu-Peng Hsiao, Yi-Ling Liu, Chiao-Hsuan Chao, Robert Anderson, Trai-Ming Yeh, Yee-Shin Lin, Shu-Wen Wan
Summary: This study developed a modified DENV vaccine candidate with enhanced features by combining modifications of two DENV proteins. The vaccine candidate showed protective effects against DENV infection in a mouse model, indicating its potential clinical application.
Article
Computer Science, Artificial Intelligence
Muhammad Abdullah Sandhu, Asjad Amin
Summary: In this study, an automated model for dengue larvae detection and tracking using CNN and Kalman filters was proposed. Water areas and dengue larvae datasets were collected, and CNN was used to detect water areas and Kalman filters were used to track the larvae. The experimental results showed excellent performance, and positive feedback was received from health authorities.
JOURNAL OF INTELLIGENT & FUZZY SYSTEMS
(2023)
Article
Multidisciplinary Sciences
Renato J. Horikoshi, Patrick M. Dourado, Geraldo U. Berger, Davi de S. Fernandes, Celso Omoto, Alan Willse, Samuel Martinelli, Graham P. Head, Alberto S. Correa
Summary: The Cry1Ac soybean technology has been widely adopted in Brazil since 2013 and effectively managed the main lepidopteran pests, but there is an increase in the relative abundance of non-target Spodoptera spp. larvae.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Guntur Fibriansah, Elisa X. Y. Lim, Jan K. Marzinek, Thiam-Seng Ng, Joanne L. Tan, Roland G. Huber, Xin-Ni Lim, Valerie S. Y. Chew, Victor A. Kostyuchenko, Jian Shi, Ganesh S. Anand, Peter J. Bond, James E. Crowe, Shee-Mei Lok
Summary: Dengue virus has four serotypes (DENV1-4) with different strains within each serotype. The virus can exhibit smooth or bumpy surface morphologies at 37 degrees C, depending on the strain. High-affinity therapeutic antibodies are able to neutralize diverse morphologies of dengue virus.
Article
Parasitology
Chandana Wijesinghe, Jagath Gunatilake, P. H. D. Kusumawathie, P. D. N. N. Sirisena, S. W. P. L. Daulagala, Bushran N. Iqbal, Faseeha Noordeen
Summary: A study conducted in a high-risk dengue area in Sri Lanka detected comparatively high rates of VT of DENV in Ae. albopictus and Ae. aegypti larvae, with a shift in the predominant DENV serotype observed from December 2015 to March 2017.
PARASITES & VECTORS
(2021)
Article
Immunology
Adrian D. Friedrich, Valeria E. Campo, Eliana M. Cela, Adrian E. Morelli, William J. Shufesky, Olga A. Tckacheva, Juliana Leoni, Mariela L. Paz, Adriana T. Larregina, Daniel H. Gonzalez Maglio
EUROPEAN JOURNAL OF IMMUNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Maria A. Toscanini, Daniel Gonzalez Maglio, Paula Capece, Gladys Posse, Cristina A. Iovannitti, Alejandro D. Nusblat, Maria L. Cuestas
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Letter
Dermatology
Fernando Pinheiro Souza-Neto, Poliana Camila Marinello, Gabriela Pasqual Melo, Leandra Zambeli Naira Ramalho, Eliana M. Cela, Valeria E. Campo, Daniel H. Gonzalez-Maglio, Rubens Cecchini, Alessandra Lourenco Cecchini
JOURNAL OF DERMATOLOGICAL SCIENCE
(2020)
Article
Peripheral Vascular Disease
Carolina Caniffi, Estefania Prentki Santos, Flavia M. Cerniello, Analia L. Tomat, Daniel Gonzalez Maglio, Jorge E. Toblli, Cristina Arranz
JOURNAL OF HYPERTENSION
(2020)
Article
Environmental Sciences
Lourdes Caceres, Mariela L. Paz, Mariana Garces, Valeria Calabro, Natalia D. Magnani, Manuela Martinefski, Pamela V. Martino Adami, Laura Caltana, Deborah Tasat, Laura Morelli, Valeria Tripodi, Giuseppe Valacchi, Silvia Alvarez, Daniel Gonzalez Maglio, Timoteo Marchini, Pablo Evelson
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2020)
Meeting Abstract
Biochemistry & Molecular Biology
Timoteo Marchini, Natalia Magnani, Mariana Garces, Kelly Jazmin, Mariela Paz, Lourdes Caceres, Romina Lasagni Vitar, Valeria Calabro, Laura Caltana, Mario Contin, Sofia Reynoso, Nestor Lago, Tamara Vico, Virginia Vanasco, Valeria Tripodi, Silvia Alvarez, Daniel Gonzalez Maglio, Bruno Buchholz, Alejandro Berra, Ricardo Gelpi, Pablo Evelson
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Mariano E. Justo, Mayra Aldecoa, Eliana Cela, Juliana Leoni, Daniel H. Gonzalez Maglio, Andres M. Villa, Florencia Aguirre, Mariela L. Paz
Summary: The study found that a high percentage of myasthenia gravis patients have insufficient levels of vitamin D, with those with more severe disease having even lower levels. This highlights a potential association between vitamin D deficiency and disease severity in myasthenia gravis patients.
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2021)
Review
Pharmacology & Pharmacy
Maria Priscila Saracino, Cecilia Celeste Vila, Pablo Cesar Baldi, Daniel Horacio Gonzalez Maglio
Summary: Helminth infections pose a significant global health concern with over one billion people affected, and despite efforts, there is no effective human vaccine. The current main drugs used to control helminths are facing increasing resistance in livestock, leading to a need for alternative treatments like plant extracts or probiotics. Probiotics, by definition, are live microorganisms that can benefit the host's health when consumed in adequate amounts, and they have been shown to have immunomodulatory effects that could help in controlling gastrointestinal helminth infections.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Environmental Sciences
Timoteo Marchini, Natalia Magnani, Mariana Garces, Jazmin Kelly, Mariela Paz, Lourdes Caceres, Valeria Calabro, Romina Lasagni Vitar, Laura Caltana, Mario Contin, Sofia Reynoso, Nestor Lago, Tamara Vico, Virginia Vanasco, Dennis Wolf, Valeria Tripodi, Daniel Gonzalez Maglio, Silvia Alvarez, Bruno Buchholz, Alejandro Berra, Ricardo Gelpi, Pablo Evelson
Summary: Exposure to air pollution is positively associated with cardiovascular morbidity and mortality rates, particularly myocardial infarction. This study investigates the metabolic mechanisms underlying this association, focusing on cardiac mitochondrial function and dynamics. The findings highlight the detrimental effects of urban air pollution on heart tissue oxygen metabolism, mitochondrial ultrastructure, and function, and emphasize the importance of considering environmental factors in the development of cardiovascular diseases.
ENVIRONMENTAL POLLUTION
(2022)
Article
Nutrition & Dietetics
Adrian D. Friedrich, Juliana Leoni, Mariela L. Paz, Daniel H. Gonzalez Maglio
Summary: Lipoteichoic acid (LTA) from different Gram-positive bacterial sources has varying immune effects. Lacticaseibacillus rhamnosus GG LTA (LGG-LTA) oral administration reduces UVB-induced immunosuppression and skin tumor development in mice. In this study, the immunomodulatory effects of LGG-LTA on dendritic cells (DC) and T cells were evaluated in vitro and in the gut-associated lymphoid tissue (GALT). The results showed that LGG-LTA activated DC and T cells, promoting Th1-biased immune response after oral LTA administration.
Article
Dermatology
Maria A. Toscanini, Carolina Rodriguez Laboccetta, Agustin Garrido, Gladys B. Posse, Paula Capece, Ruth M. Valdez, Yone A. Chacon, Daniel Gonzalez Maglio, Alejandro D. Nusblat, Maria L. Cuestas
Summary: This study describes a novel ELISA for antibodies testing and a dot blot assay for antigen testing using Hcp100 protein and their polyclonal antibodies, respectively, to diagnose PDH. The results showed that both methods have high sensitivity and specificity for detecting PDH and can serve as valuable screening tools.
Article
Chemistry, Multidisciplinary
Nadia Gruber, Liliana Fernandez-Canigia, Natalia B. Kilimciler, Pierluigi Stipa, Juan A. Bisceglia, Maria B. Garcia, Daniel H. Maglio, Mariela L. Paz, Liliana R. Orelli
Summary: In this study, we conducted an in-depth investigation into the activity of amidinoquinoxaline N-oxides 1 against Gram-positive and Gram-negative anaerobic bacteria. We found that these compounds showed good to excellent antianaerobic activity, low cytotoxicity, and potential as oral drug candidates.
Meeting Abstract
Biochemistry & Molecular Biology
T. Marchini, N. Magnani, M. Garces, J. Kelly, M. Paz, L. Caltana, M. Contin, N. Lago, L. Caceres, D. Margiottiello, V. Calabro, T. Vico, V. Vanasco, V. Tripodi, S. Alvarez, B. Buchholz, D. Gonzalez Maglio, A. Berra, R. J. Gelpi, P. Evelson
FREE RADICAL BIOLOGY AND MEDICINE
(2019)
