Article
Multidisciplinary Sciences
Hailian Wu, Haisheng Wu, Yongshun Wang, Hongying Li, Fuzhang Tian, Kuizhang Zhou, Zhizhen Qi, Yiquan Zhang, Qingwen Zhang, Xuefei Zhang
Summary: This study developed a Himalayan marmot information collection system based on 3S technology and V3.0, and drew a spatial distribution map of Himalayan marmots in Qinghai Province. The accuracy of the map was validated through field data collection, leading to improved efficiency in plague surveillance and reduced workload for researchers.
SCIENTIFIC REPORTS
(2023)
Review
Biochemical Research Methods
Blanca H. Lapizco-Encinas
Summary: This review article discusses the evolution of insulator-based dielectrophoresis (iDEP) and insulator-based electrokinetic (iEK) systems, highlighting recent studies that suggest particle trapping in these systems is mainly due to a balance between electroosmotic and electrophoretic effects under DC and low-frequency AC potentials, rather than dielectrophoresis. The proposed renaming of the field from DC-iDEP to DC-iEK reflects the shift in understanding particle manipulation and separation mechanisms in these systems.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Zhenhua Wen, Xuelian Hu, Rongfang Yan, Wenxiu Wang, He Meng, Yang Song, Shuo Wang, Xianghong Wang, Yiwei Tang
Summary: An immunoassay strategy integrating a UCNP-based immunochromatographic strip with a fluorescence reader was proposed for the detection of olaquindox residues. The method achieved specific recognition and sensitive response to olaquindox. The approach demonstrated a favorable linear range and sensitive detection, and was successfully applied to determine olaquindox in fish muscle and water samples.
Article
Infectious Diseases
Jianyun Li, Yumeng Wang, Fang Liu, Xiaona Shen, Yiting Wang, Mengguang Fan, Yao Peng, Shuyi Wang, Yilan Feng, Wen Zhang, Yanning Lv, Huijuan Zhang, Xin Lu, Enmin Zhang, Jianchun Wei, Lijuan Chen, Biao Kan, Zhongbing Zhang, Jianguo Xu, Wenrui Wang, Wei Li
Summary: The study highlights the significant threat of plague to human health, as seen in the plague outbreaks in Madagascar in 2017 and Inner Mongolia in 2019. By utilizing genome-wide SNP analysis and PCR-based molecular subtyping, the research identifies that local rodent plague epizootics are the source of human plague cases and emphasizes the importance of early warning and risk communication due to the lack of geographic barriers between the plague foci in Inner Mongolia and Mongolia.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Multidisciplinary Sciences
Aida Andrades Valtuena, Gunnar U. Neumann, Maria A. Spyrou, Lyazzat Musralina, Franziska Aron, Arman Beisenov, Andrey B. Belinskiy, Kirsten I. Bos, Alexandra Buzhilova, Matthias Conrad, Leyla B. Djansugurova, Miroslav Dobes, Michal Ernee, Javier Fernandez-Eraso, Bruno Frohlich, Miroslaw Furmanek, Agata Haluszko, Svend Hansen, Eadaoin Harney, Alina N. Hiss, Alexander Hubner, Felix M. Key, Elmira Khussainova, Egor Kitov, Alexandra O. Kitova, Corina Knipper, Denise Kuhnert, Carles Lalueza-Fox, Judith Littleton, Ken Massy, Alissa Mittnik, Jose Antonio Mujika-Alustiza, Inigo Olalde, Luka Papac, Sandra Penske, Jaroslav Peska, Ron Pinhasi, David Reich, Sabine Reinhold, Raphaela Stahl, Harald Stauble, Rezeda I. Tukhbatova, Sergey Vasilyev, Elizaveta Veselovskaya, Christina Warinner, Philipp W. Stockhammer, Wolfgang Haak, Johannes Krause, Alexander Herbig
Summary: A study reveals the dynamic nature of early evolution and ecology of the bacterial pathogen Yersinia pestis, which caused devastating outbreaks throughout human history. The study presents 17 ancient Y. pestis genomes dating back 5,000 to 2,500 years ago, showing correlations between temporal, geographical, and genetic distance and providing clues on its early evolution and potential adaptation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
David Balderas, Erin Mettert, Hanh N. Lam, Rajdeep Banerjee, Tomas Gverzdys, Pablo Alvarez, Geetha Saarunya, Natasha Tanner, Adam Zoubedi, Yahan Wei, Patricia J. Kiley, Victoria Auerbuch
Summary: The research revealed the significance of the iron-sulfur cluster regulator IscR in pathogenic Yersinia, showing its ability to regulate multiple pathways related to iron metabolism, reactive oxygen species metabolism, and cell envelope remodeling, contributing to bacterial survival during infection.
Article
Infectious Diseases
Saber Esmaeili, Ahmad Mahmoudi, Parisa Esmaeili, Zohreh Yousefi Ghalejoogh, Alireza Mordadi, Ahmad Ghasemi, Ali Mohammadi, Amin Bagheri, Aria Sohrabi, Mina Latifian, Minoarisoa Rajerison, Javier Pizarro-Cerda, Ehsan Mostafavi
Summary: This study conducted a large-scale investigation of Yersinia pestis infection in shepherd dogs, rodents, and their fleas in old foci for plague in Western Iran. The results showed serological evidence of Y. pestis infection, indicating the ongoing presence of plague in this region and the need for continuous surveillance.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Chemistry, Analytical
Bin Feng, Liyuan Shi, Haipeng Zhang, Haimei Shi, Chuanfan Ding, Peng Wang, Shaoning Yu
Summary: This study established a complete MALDI-TOF MS data pipeline and identified a Y. pestis-specific biomarker, achieving nearly perfect separation between Y. pseudotuberculosis and Y. pestis with a separation accuracy of 0.999 using an LDA model. The new computing method paves the way for automatic differentiation between the two highly similar bacterial species.
Editorial Material
Biochemistry & Molecular Biology
Verena J. Schuenemann
Summary: Ancient DNA methodologies have the potential to study the past prevalence and evolutionary history of pathogens. A recent study identified bacteria that cause plague and typhoid fever in Minoan Crete, illustrating both the possibilities and limitations of ancient pathogen genomics.
Article
Multidisciplinary Sciences
Magdalena Haller, Kimberly Callan, Julian Susat, Anna Lena Flux, Alexander Immel, Andre Franke, Alexander Herbig, Johannes Krause, Anne Kupczok, Gerhard Fouquet, Susanne Hummel, Dirk Rieger, Almut Nebel, Ben Krause-Kyora
Summary: Archaeological findings in a mass burial site in Lubeck, Germany revealed over 800 skeletal remains, with DNA analysis suggesting the cause of death to be enteric paratyphoid fever between 1270 and 1400 CE. The reconstructed Salmonella Paratyphi C genomes showed similarity to a strain from Norway in 1200 CE, indicating likely trade connections in the Baltic region during the Late Medieval period.
Article
Chemistry, Analytical
Feng-Ping Lin, Hui-Ling Hsu, Pei-Yi Tsui, Chung-Chih Liang, Chien-Hsing Lu, Jem-Kun Chen
Summary: A simple method for detecting pathogens in human blood specimens quickly at the point of care was developed to prevent the widespread transmission of communicable diseases, with Yersinia pestis as the first target. The system achieved high sensitivity and specificity, based on one-dimensional diffraction grating (ODG) chips and laser beams. It was portable and could complete the entire process within minutes at the point of care.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Multidisciplinary Sciences
Diego Leandro Reis da Silva Fernandes, Elainne Christine de Souza Gomes, Matheus Filgueira Bezerra, Ricardo Jose de Paula Souza e Guimaraes, Alzira Maria Paiva de Almeida
Summary: This study utilized satellite data and historical files to analyze the spatial and temporal distribution of human plague cases in Pernambuco, Northeastern Brazil. The findings revealed a shift of infection from urban to wild-sylvatic areas and the reemergence of cases after a period of quiescence.
Article
Multidisciplinary Sciences
Galina A. Eroshenko, Nikolay V. Popov, Zhanna V. Al'khova, Lyubov M. Kukleva, Alina N. Balykova, Nadezhda S. Chervyakova, Ekaterina A. Naryshkina, Vladimir V. Kutyrev
Summary: The study identified distinct phylogenetic branches in Yersinia pestis strains isolated in the Northern Caspian and Northern Aral Sea regions, with the early 2.MED4 branch preceding the later 2.MED1 branch. The 2.MED1 branch emerged as the etiological agent of plague outbreaks in the early 20th century and spread to the Caspian Sea region, Caucasus, and Central Asia in the later part of the 20th century.
Review
Biochemistry & Molecular Biology
Raysa Rosario-Acevedo, Sergei S. Biryukov, Joel A. Bozue, Christopher K. Cote
Summary: Plague, caused by the bacterial pathogen Yersinia pestis, is a vector-borne disease that continues to infect humans worldwide. Research on vaccines and therapeutics for plague is important for infection mitigation and disease treatment, as plague remains a public health threat and biodefense concern.
Article
Immunology
Guillain Mikaty, Christian E. Demeure, Sofia Filali, Javier Pizarro-Cerda, Pierre Goossens, Elisabeth Carniel
Summary: This study aimed to determine whether DIC occurs at the late stages of plague and anthrax. The results showed that animals infected with anthrax exhibited typical DIC characteristics, while those infected with plague did not. Therefore, understanding the mechanisms behind these hemorrhages is crucial.
MICROBES AND INFECTION
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)