期刊
BIORESOURCE TECHNOLOGY
卷 293, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.122098
关键词
Scleroglucan; pH-shift strategy; Fed-batch; Sclerotium rolfsii; Overproduction
资金
- National Key Research and Development Program of China [2017YFC1600403]
- National Science Fund for Excellent Young Scholars [21822806]
- Fundamental Research Funds for the Central Universities [JUSRP51701A]
- National First-class Discipline Program of Light Industry Technology and Engineering [LITE2018-08]
- Distinguished Professor Project of Jiangsu Province
As a stable microbial polysaccharide, scleroglucan has extensive application in the food, medicine, and cosmetics industries. However, its large-scale industrial application is limited by its high production cost, low yield, long production time, etc. This study aims to enhance scleroglucan production by Sclerotium rolfsii WSH-G01. Based on the analysis of batch fermentation kinetics parameters, a pH-shift strategy was adopted. Through systematic kinetics analysis, a 32.4 g/L scleroglucan was accomplished. The kinetic model of the pH-shift batch fermentation process was established using a logistic equation, Luedeking-Piret equation, and a Luedeking-Piret-like equation. As decreased glucose concentration could cause decreased scleroglucan synthesis rates during the batch fermentation process, 30 g/L glucose was fed in the later phase of fermentation. As a result, scleroglucan production increased to 42 g/L, with a productivity of 0.5 g/L.h. Thus, the pH-shift strategy and feeding approach could be useful for industrial scleroglucan production.
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