期刊
BIORESOURCE TECHNOLOGY
卷 293, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.122054
关键词
Ashbya gossypii; Microbial lipids; Regulation; Lignocellulosic hydrolysates; Xylose; Molasses; Glycerol
资金
- Spanish Ministerio de Economia y Competitividad [BIO2014-56930-P, BIO2017-88435-R]
- Junta de Castilla y Leon [SA016P17]
- Portuguese Foundation for Science and Technology (FCT) [UID/BIO/04469/2019, PD/BD/113812/2015]
- European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte [NORTE-01-0145-FEDER-000004, POCI-01-0145-FEDER-016403]
- USAL predoctoral fellowship from the University of Salamanca
- Fundação para a Ciência e a Tecnologia [PD/BD/113812/2015] Funding Source: FCT
This work presents the exploitation of waste industrial by-products as raw materials for the production of microbial lipids in engineered strains of the filamentous fungus Ashbya gossypii. A lipogenic xylose-utilizing strain was used to apply a metabolic engineering approach aiming at relieving regulatory mechanisms to further increase the biosynthesis of lipids. Three genomic manipulations were applied: the overexpression of a feedback resistant form of the acetyl-CoA carboxylase enzyme; the expression of a truncated form of Mga2, a regulator of the main Delta 9 desaturase gene; and the overexpression of an additional copy of DGA1 that codes for diacylglycerol acyltransferase. The performance of the engineered strain was evaluated in culture media containing mixed formulations of corn-cob hydrolysates, sugarcane molasses or crude glycerol. Our results demonstrate the efficiency of the engineered strains, which were able to accumulate about 40% of cell dry weight (CDW) in lipid content using organic industrial wastes as feedstocks.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据