期刊
BIOLOGY OF REPRODUCTION
卷 102, 期 4, 页码 975-983出版社
OXFORD UNIV PRESS INC
DOI: 10.1093/biolre/ioaa002
关键词
spermatogenesis; meiotic arrest; male infertility; knockout
资金
- Ministry of Education, Culture, Sports, Science and Technology (MEXT)/Japan Society for the Promotion of Science (JSPS) KAKENHI [JP15J04519, JP15H05573, JP15K14367, JP18K14715, JP17H01394]
- Japan Agency for Medical Research and Development grant [JP18gm5010001]
- Eunice Kennedy Shriver National Institute of Child Health and Human Development [P01HD087157, R01HD088412]
- Bill & Melinda Gates Foundation [OPP1160866]
In mammals, more than 2000 genes are specifically or abundantly expressed in testis, but gene knockout studies revealed several are not individually essential for male fertility. Tesmin (Metallothionein-like 5; Mtl5) was originally reported as a testis-specific transcript that encodes a member of the cysteine-rich motif containing metallothionein family. Later studies showed that Tesmin has two splicing variants and both are specifically expressed in male and female germ cells. Herein, we clarified that the long (Tesmin-L) and short (Tesmin-S) transcript forms start expressing from spermatogonia and the spermatocyte stage, respectively, in testis. Furthermore, while Tesmin-deficient female mice are fertile, male mice are infertile due to arrested spermatogenesis at the pachytene stage. We were able to rescue the infertility with a Tesmin-L transgene, where we concluded that TESMIN-L is critical for meiotic completion in spermatogenesis and indispensable for male fertility. Summary sentence TESMIN protein, a member of metallothionein family, is essential for mouse spermatogenesis, especially in the meiotic stage.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据