Article
Microbiology
Pengyu Luan, Yanjie Yi, Yifan Huang, Liuqing Cui, Zhipeng Hou, Lijuan Zhu, Xiujuan Ren, Shao Jia, Yang Liu
Summary: This study investigated the antagonistic effect of a bacterium against Bipolaris sorokiniana and found that it effectively inhibited the pathogen and promoted wheat growth.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Pengjie He, Wenyan Cui, Pengbo He, Shahzad Munir, Xingyu Li, Yixin Wu, Yongmei Li, Suhail Asad, Pengfei He, Yueqiu He
Summary: The Bacillus amyloliquefaciens subsp. plantarum strain KC-1 has been identified as an effective biocontrol agent against Pectobacterium carotovora subsp. carotovora, significantly reducing soft rot disease in Zantedeschia species. Application of KC-1 resulted in persistent presence on leaves for up to 30 days and maximum inhibition activity in greenhouse conditions. Pre-treatment with KC-1 showed higher growth levels and reduced disease severity index in Zantedeschia tubers.
BIOLOGICAL CONTROL
(2021)
Article
Biotechnology & Applied Microbiology
Qingxia Zhang, Yinyin Liu, Paul R. Harvey, Belinda E. Stummer, Jinglong Yang, Zhaolin Ji
Summary: The study developed quantitative assays to determine the rhizosphere competence of Bacillus amyloliquefaciens W10 and Pseudomonas protegens FD6, and their suppressive efficacies against Rhizoctonia cerealis. The qPCR assay proved to be an effective method to measure the abundance of these strains in wheat seedlings.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Plant Sciences
Jing Nan, Shaoran Zhang, Ling Jiang
Summary: The study showed that treatment with Bacillus amyloliquefaciens GJ1 can improve citrus immunity by increasing photosynthesis and enhancing the expression of resistance-related genes.
Article
Agronomy
Wan-Qiong Ye, Ya-Fang Sun, Ya-Jie Tang, Wen-Wen Zhou
Summary: Bacillus amyloliquefaciens B4 is a promising biological agent for controlling postharvest diseases of loquat fruit. It shows broad-spectrum antifungal activity and is non-toxic in oral toxicity tests, making it a suitable biocontrol agent. Treatment with B4 results in lower disease incidence compared to untreated fruit.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2021)
Article
Agronomy
Vahida Rahman, Khem Raj Meena, Laith Khalil Tawfeeq Al-Ani, Ashutosh Singh, Arvind Kumar
Summary: In this study, random mutations were induced in Bacillus velezensis to enhance its biocontrol capacity against plant pathogens. Mutant strains showed increased production of biosurfactants and improved antagonistic activity against Helminthosporium maydis. Pot experiments demonstrated significant improvement in maize growth promotion and disease suppression. These findings suggest the potential of mutated B. velezensis strains as biocontrol agents for southern corn leaf blight.
EUROPEAN JOURNAL OF PLANT PATHOLOGY
(2023)
Article
Plant Sciences
Kun Kang, Zhipeng Niu, Wei Zhang, Shan Wei, Yangyong Lv, Yuansen Hu
Summary: Common root rot caused by Bipolaris sorokiniana infestation is a major reason for reduced wheat yield worldwide. Bacillus halotolerans strain JK-25, isolated from wheat rhizosphere soil, shows significant antagonism against B. sorokiniana and other pathogens. The surfactin produced by JK-25 inhibits B. sorokiniana growth and disrupts its cell membranes. In pot experiments, the addition of JK-25 culture fermentation supernatant significantly reduces the incidence of root rot, indicating its potential as a biological control agent.
Article
Microbiology
Lin-Jie Nie, Wan-Qiong Ye, Wan-Yue Xie, Wen-Wen Zhou
Summary: The biofilm of Bacillus amyloliquefaciens plays an important role in the management of postharvest fruit decay and can be regulated by metal compounds. This finding provides a new strategy for the preservation of postharvest fruit.
MICROBIOLOGICAL RESEARCH
(2022)
Review
Microbiology
Mohamad Syazwan Ngalimat, Radin Shafierul Radin Yahaya, Mohamad Malik Al-adil Baharudin, Syafiqah Mohd Yaminudin, Murni Karim, Siti Aqlima Ahmad, Suriana Sabri
Summary: Members of the operational group Bacillus amyloliquefaciens (OGBa) are Gram-positive, endospore-forming, rod-shaped bacteria that have potential applications as plant growth promoters, biocontrol agents, probiotics, bioremediation agents, as well as producers of commercial enzymes and antibiotics. They show promise in removing organophosphorus pesticides.
Article
Microbiology
Yanjie Yi, Youtian Shan, Shifei Liu, Yanhui Yang, Yang Liu, Yanan Yin, Zhipeng Hou, Pengyu Luan, Ruifang Li
Summary: The study showed that Bacillus amyloliquefaciens XZ34-1 strain can produce antifungal bioactive substances with significant inhibitory effects against Bipolaris sorokiniana, reducing the incidence and disease index of common root rot in wheat, and improving the efficacy of biological control.
Article
Agronomy
Hau-Ping Chou, Yi-Chiao Huang, Yi-Hsien Lin, Wen-Ling Deng
Summary: A Bacillus amyloliquefaciens strain PMB01 was found to effectively inhibit the growth of Ralstonia solanacearum and reduce bacterial wilt in tomatoes, especially when supplemented with 1% soybean meal in the culture medium.
Article
Microbiology
Qiubin Huang, Huiping Liu, Juanmei Zhang, Shaowei Wang, Fengying Liu, Chengdie Li, Gang Wang
Summary: Bacillus cereus 0-9 encodes three genes annotated as amylases, among which only the amyS gene produces extracellular amylase. This gene influences the colonization abilities of the bacterium in wheat roots and its biocontrol abilities against Rhizoctonia cerealis.
Article
Microbiology
Fan Mu, Xu Chen, Zhenxin Fu, Xue Wang, Jiexin Guo, Xiaojun Zhao, Baojun Zhang
Summary: This study identified a B. amyloliquefaciens strain XJ5 as a potential biocontrol agent against the phytopathogen A. solani. The inhibition of A. solani growth was achieved through the secretion of antagonistic secondary metabolites and hydrolases by XJ5. Transcriptome analysis revealed the downregulation of genes related to chitin and mannose synthesis in A. solani when exposed to XJ5 crude protein extracts, suggesting the impact of XJ5 metabolites on these pathways.
Article
Immunology
Lingxiao Cui, Chengde Yang, Yinyu Wang, Ting Ma, Fengfeng Cai, Lijuan Wei, Mengjun Jin, Richard Osei, Junlian Zhang, Mei Tang
Summary: The endophytic bacterium B. amyloliquefaciens 3-5, isolated from healthy potato tubers, shows antagonistic activity against the pathogen causing potato scab. It has the ability to produce indole-3-acetic acid (IAA) and fix nitrogen. PCR detection revealed that B. amyloliquefaciens 3-5 possesses several antibacterial substance synthesis genes.
MICROBIAL PATHOGENESIS
(2022)
Article
Plant Sciences
Rui Wang, Chunwei Wang, Bing Zuo, Xinyuan Liang, Danni Zhang, Renxuan Liu, Lina Yang, Baohui Lu, Xue Wang, Jie Gao
Summary: The biocontrol efficacy of Bacillus amyloliquefaciens FS6 against seedling diseases and gray mold of ginseng was evaluated, showing strong antagonistic effects on fungal pathogens and long-lasting protection. The combination of seed and soil treatments exhibited better protection, with FS6 wettable powder soil treatment in combination with thiamethoxam plus metalaxyl-M plus fludioxonil performing the best. FS6 also reduced the diversity of fungi and affected the fungi and bacterial composition in the rhizosphere soil of ginseng.
Article
Biotechnology & Applied Microbiology
Pengjie He, Wenyan Cui, Pengbo He, Shahzad Munir, Xingyu Li, Yixin Wu, Yongmei Li, Suhail Asad, Pengfei He, Yueqiu He
Summary: The Bacillus amyloliquefaciens subsp. plantarum strain KC-1 has been identified as an effective biocontrol agent against Pectobacterium carotovora subsp. carotovora, significantly reducing soft rot disease in Zantedeschia species. Application of KC-1 resulted in persistent presence on leaves for up to 30 days and maximum inhibition activity in greenhouse conditions. Pre-treatment with KC-1 showed higher growth levels and reduced disease severity index in Zantedeschia tubers.
BIOLOGICAL CONTROL
(2021)
Article
Biotechnology & Applied Microbiology
Shuwen Bu, Shahzad Munir, Pengfei He, Yongmei Li, Yixin Wu, Xingyu Li, Baohua Kong, Pengbo He, Yueqiu He
Summary: Gray mold (Botrytis cinerea) is a serious disease that causes significant losses to harvested tomatoes during storage and transportation. The trend is shifting towards prevention and control measures like biological control to ensure environmental protection. Bacillus subtilis L1-21 has shown potential as a biocontrol agent in controlling gray mold and other postharvest diseases during storage time.
BIOLOGICAL CONTROL
(2021)
Review
Biotechnology & Applied Microbiology
Shahzad Munir, Ayesha Ahmed, Yongmei Li, Pengbo He, Brajesh K. Singh, Pengfei He, Xingyu Li, Suhail Asad, Yixin Wu, Yueqiu He
Summary: Huanglongbing has devastated the citrus industry globally, prompting large-scale research into engineering microbiomes through indigenous endophytes as a sustainable cure. This revolutionary approach is not only applicable to citrus-HLB, but also for tailoring customized solutions for general plant-pathogen systems affected by vascular system-restricted pathogens.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2022)
Article
Immunology
Rong Jiao, Yongzhan Cai, Pengfei He, Shahzad Munir, Xingyu Li, Yixin Wu, Junwei Wang, Mengyuan Xia, Pengbo He, Ge Wang, Huanwen Yang, Samantha C. Karunarathna, Yan Xie, Yueqiu He
Summary: Bacillus amyloliquefaciens YN201732 has been found to have significant antifungal activity against tobacco powdery mildew, with bacillomycin D and fengycin playing potential roles in this process. However, gene mutations related to lipopeptide synthesis may also impact the biofilm formation of strains.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Zuxian Pan, Shahzad Munir, Yongmei Li, Pengbo He, Pengfei He, Yixin Wu, Yan Xie, Zongwei Fu, Yongzhan Cai, Yueqiu He
Summary: The study identified potential biocontrol agents that can effectively control tobacco leaf mildew, with B. amyloliquefaciens B9601-Y2 showing the best inhibitory effect on the fungal pathogen. Different application methods of this bacterial strain also demonstrated high control effects in preventing mildew on tobacco leaves during the curing process.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Shahzad Munir, Yongmei Li, Pengbo He, Pengfei He, Pengjie He, Wenyan Cui, Yixin Wu, Xingyu Li, Qi Li, Sixiang Zhang, Yangsu Xiong, Zhanjun Lu, Wenbiao Wang, Kexian Zong, Yongchao Yang, Shaocong Yang, Chan Mu, Heming Wen, Yuehu Wang, Jun Guo, Samantha C. Karunarathna, Yueqiu He
Summary: Huanglongbing (HLB) is a devastating botanical pandemic of citrus crops caused by Candidatus Liberibacter asiaticus (CLas), for which there is no cure currently available. However, a research study has found that introducing an indigenous endophyte Bacillus subtilis L1-21 isolated from healthy citrus can help manage the disease. The study showed that application of B. subtilis L1-21 resulted in a significant reduction in CLas copies in infected citrus trees and orchards over a period of 2 years.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Microbiology
Yongmei Li, Mengyuan Xia, Pengbo He, Qiaoming Yang, Yixin Wu, Pengfei He, Ayesha Ahmed, Xiangsong Li, Yuehu Wang, Shahzad Munir, Yueqiu He
Summary: In this study, the biocontrol activity of endophyte Bacillus subtilis L1-21 against citrus green mold caused by Penicillium digitatum was evaluated. The results showed that B. subtilis L1-21 inhibited the pathogen and produced potential antifungal compounds. Moreover, B. subtilis L1-21 could easily colonize in citrus fruit and eliminate the fungal pathogen.
Article
Chemistry, Medicinal
Lu Zhang, Ping Yi, Hui Yan, Xiao-Nian Li, Meng-Yuan Xia, Jun Yang, Ji-Feng Luo, Yue-Qiu He, Yue-Hu Wang
Summary: Chemical components isolated from the heartwood of Aquilaria sinensis were identified using various analytical techniques. Five new 2-(2-phenylethyl)chromone derivatives and three new sesquiterpenoids were discovered, and their neuroprotective activities were evaluated.
NATURAL PRODUCTS AND BIOPROSPECTING
(2022)
Article
Biotechnology & Applied Microbiology
Pengjie He, Wenyan Cui, Ling Peng
Summary: The Bacillus velezensis strain HC-8 showed high antifungal activity against powdery mildew on honeysuckle plants, with the best biocontrol efficacy observed when applied before pathogen inoculation. This strain also induced defense-related enzyme activities in honeysuckle leaves, providing a potential high-efficiency strategy for biological control.
BIOLOGICAL CONTROL
(2022)
Article
Biochemistry & Molecular Biology
Wenyan Cui, Pengjie He
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Article
Cell Biology
Pengjie He, Wenyan Cui, Shahzad Munir, Pengbo He, Ruirong Huang, Xingyu Li, Yixin Wu, Yuehu Wang, Jing Yang, Ping Tang, Yueqiu He, Pengfei He
Summary: This study demonstrates that Bacillus subtilis XF-1 can efficiently control clubroot disease of cruciferous crops through leaf spraying. The production of fengycin-type cyclopeptides by XF-1 plays a crucial role in dissolving testing spores and infecting root hairs. It also enhances plant defense reactions by activating the jasmonic acid and salicylic acid signaling pathways.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Pengjie He, Xiaodi Tang, Shenghua Wei, Jiangyong An, Wenyan Cui
Summary: Bacillus velezensis TH-1, a plant growth-promoting rhizobacteria, was isolated from the rhizosphere of Sophora tonkinensis Radix and has potential biocontrol activity against root rot and bacterial wilt. The genome sequencing of TH-1 revealed a size of 3,929,846 bp with 3,900 genes and a GC content of 46.48%. The study also identified nine gene clusters responsible for antimicrobial compound biosynthesis. These genomic resources will contribute to the understanding of biological control mechanisms and plant-microbe interactions.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2023)
Article
Chemistry, Multidisciplinary
Xingyu Li, Shahzad Munir, Yan Xu, Yuehu Wang, Yueqiu He
Summary: By performing a comprehensive analysis, various acaricidal activity ingredients were identified in Bacillus velezensis W1, providing a foundation for studying its mechanism of action and for further research on active ingredients from biocontrol strains.