Review
Biochemistry & Molecular Biology
Anton O. Chugunov, Nadezhda A. Potapova, Natalia S. Klimenko, Victor V. Tatarskiy, Sofia G. Georgieva, Nataliya Soshnikova
Summary: The DPF domain of PHF10 is important for transcription activation, although its structure remains undetermined. Our homology modeling revealed common and distinct features in structure and histone modifications recognition capabilities of the PHF10 DPF domain, which may impact the recruitment of the PBAF complex to chromatin. Additionally, we traced the evolution of DPF1-3 and PHF10 genes from unicellular to vertebrate organisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Theodore Busby, Yuechuan Chen, Tanner C. Godfrey, Mohammad Rehan, Benjamin J. Wildman, Caris M. Smith, Quamarul Hassan
Summary: This article investigates tissue-specific regulation in mineralized tissues through chromatin remodeling, focusing on the expression of Baf45a and Baf45d. The study finds that Baf45a and Baf45d are preferentially expressed in osteoblasts and odontoblasts, and their expression is associated with the differentiation and maturation of these cells. The research also reveals the importance of the interaction between BAF45A, PBAF subunits, and the transcription factor RUNX2 in regulating gene expression in mineralized tissues.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cell Biology
Nataliya V. Soshnikova, Asya M. Azieva, Nataliya S. Klimenko, Alvina I. Khamidullina, Alexey V. Feoktistov, Andrey A. Sheynov, Alexander V. Brechalov, Victor V. Tatarskiy, Sofia G. Georgieva
Summary: dcPBAF, a variant of the PBAF complex, is present in differentiated neuronal cells and is responsible for the transcription of specific neuron-specific and housekeeping genes in adult neurons.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
N. V. Soshnikova, E. V. Tatarskiy, V. V. Tatarskiy, N. S. Klimenko, A. A. Shtil, M. A. Nikiforov, S. G. Georgieva
Summary: The PBAF complex, with its PHF10 subunit, plays a crucial role in transcriptional regulation and is associated with disease progression in melanoma. The interaction between PHF10 and MYC enhances MYC's activation of genes involved in cell cycle progression. Depletion of PHF10 or MYC leads to G1 accumulation and a senescence-like phenotype in melanoma cells.
Article
Biophysics
Charles A. Kenworthy, Nayem Haque, Shu-Hao Liou, Panagiotis Chandris, Vincent Wong, Patrycja Dziuba, Luke D. Lavis, Wei-Li Liu, Robert H. Singer, Robert A. Coleman
Summary: Chromatin remodeler PBAF can dynamically target different chromatin states within a live cell and cycles on and off chromatin hubs, with bromodomains potentially playing a direct role in nucleosome remodeling.
BIOPHYSICAL JOURNAL
(2022)
Article
Immunology
Amy E. Baxter, Hua Huang, Josephine R. Giles, Zeyu Chen, Jennifer E. Wu, Sydney Drury, Katherine Dalton, Simone L. Park, Leonel Torres, Brandon W. Simone, Max Klapholz, Shin Foong Ngiow, Elizabeth Freilich, Sasikanth Manne, Victor Alcalde, Viktoriya Ekshyyan, Shelley L. Berger, Junwei Shi, Martha S. Jordan, John Wherry
Summary: The study reveals the roles of two forms of the SWI/SNF chromatin-remodeling complex in CD8+ T cell exhaustion. PBAF promotes Tex-cell proliferation and survival, while BAF is necessary for the generation of effector-like Tex cells. Balancing the activities of these complexes coordinates Tex-cell subset differentiation.
Article
Cell & Tissue Engineering
Jiayi Chen, Guangqin Liu, Xinzheng Wang, Hao Hong, Tingting Li, Lin Li, Hongxiang Wang, Jiong Xie, Bohan Li, Ting Li, Dingyi Lu, Yakun Zhang, Haixin Zhao, Chengcheng Yao, Kaiqing Wen, Teng Li, Jing Chen, Shengming Wu, Kun He, Wei-Na Zhang, Jie Zhao, Na Wang, Qiuying Han, Qing Xia, Ji Qi, Juxiang Chen, Tao Zhou, Jianghong Man, Xue-Min Zhang, Ai-Ling Li, Xin Pan
Summary: This study explores the communication between glioblastoma stem cells (GSCs) and the microenvironment, revealing that GSCs secrete histamine to shape a pro-angiogenic tumor microenvironment. The secretion of histamine by GSCs is associated with the expression and modification of the HDC gene, and the histamine activates endothelial cells through the H1R-Ca2+-NF-kB axis, promoting angiogenesis and GBM progression. The findings suggest that targeting histamine could be a potential strategy for GBM therapy.
Article
Biochemistry & Molecular Biology
A. M. Azieva, A. A. Sheynov, D. A. Kirillova, E. V. Tatarskiy, S. G. Georgieva, N. V. Soshnikova
Summary: The PBAF chromatin remodeling complex plays a crucial role in maintaining and modifying chromatin structure in mammalian cells. The PHF10 subunit interacts with the c-FOS transcription factor and is involved in neuronal development and the establishment of long-term contacts between neurons during LTP induction.
Article
Oncology
Borwyn A. Wang, Hrishikesh M. Mehta, Srinivasa R. Penumutchu, Blanton S. Tolbert, Chonghui Cheng, Marek Kimmel, Torsten Haferlach, Jaroslaw P. Maciejewski, Seth J. Corey
Summary: This study discovers two different isoforms of CSF3R in myelodysplastic syndromes and identifies the role of the splicing factor SRSF2 in regulating their expression, which affects granulopoiesis.
Article
Biochemistry & Molecular Biology
Yuri Prozzillo, Stefano Cuticone, Diego Ferreri, Gaia Fattorini, Giovanni Messina, Patrizio Dimitri
Summary: The chromatin organization in Drosophila melanogaster is regulated by Tip60 chromatin remodeling complex (dTip60), DOM-A.C and DOM-B.C complexes, which share most subunits. This study combined genetics and cell biology to investigate the biological roles of these subunits in Drosophila melanogaster development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Elizabeth T. Wiles, Colleen C. Mumford, Kevin J. McNaught, Hideki Tanizawa, Eric U. Selker
Summary: Establishing and maintaining appropriate gene repression is crucial for multicellular organisms. H3K27 methylation is a common modification associated with gene repression, and recent research has identified the involvement of ISWI and ACF1 genes in the repression of H3K27-methylated genes in the filamentous fungus Neurospora crassa. These genes form an ACF chromatin-remodeling complex and interact with chromatin throughout the genome. Proper nucleosome positioning is critical for the repression of H3K27-methylated genes.
Review
Biochemistry & Molecular Biology
Guohui Qin, Shasha Liu, Li Yang, Weina Yu, Yi Zhang
Summary: Variation in myeloid cell differentiation is common in tumors, inflammation, autoimmune diseases, and metabolic diseases. Single-cell sequencing reveals the differentiation trajectory of myeloid cells in COVID-19 patients. Targeting myeloid cell-derived cytokines or checkpoints is crucial in developing therapeutic strategies for severe COVID-19 patients.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)
Article
Engineering, Biomedical
Xinxin Xu, He Zhang, Yuzhou Li, Fengyi Liu, Zheng Jing, Mingxing Ren, Tao Chen, Yiru Fu, Yanqiu Wu, Ping Ji, Sheng Yang
Summary: This study investigates the effect of matrix stiffness on DNA accessibility and cell differentiation. It was found that a stiff matrix promotes osteogenic differentiation by activating the Wnt pathway, while a soft matrix leads to chromatin condensation and downregulation of beta-catenin target genes. Experimental manipulation of histone acetylation and lamin A/C expression successfully activated beta-catenin signaling in cells cultured on a soft matrix.
MATERIALS TODAY BIO
(2023)
Article
Multidisciplinary Sciences
Evan W. Weber, Kevin R. Parker, Elena Sotillo, Rachel C. Lynn, Hima Anbunathan, John Lattin, Zinaida Good, Julia A. Belk, Bence Daniel, Dorota Klysz, Meena Malipatlolla, Peng Xu, Malek Bashti, Sabine Heitzeneder, Louai Labanieh, Panayiotis Vandris, Robbie G. Majzner, Yanyan Qi, Katalin Sandor, Ling-Chun Chen, Snehit Prabhu, Andrew J. Gentles, Thomas J. Wandless, Ansuman T. Satpathy, Howard Y. Chang, Crystal L. Mackall
Summary: This study showed that transient cessation of chimeric antigen receptor (CAR) signaling can enhance the efficacy of CAR-T cells, reactivating their anti-tumor functions, and challenging the notion that exhaustion is an epigenetically fixed state.
Article
Environmental Sciences
Jianhui Liu, Xiangyang Li, Guiqing Zhou, Yue Zhang, Yujian Sang, Ji Wang, Yanbo Li, Wei Ge, Zhiwei Sun, Xianqing Zhou
Summary: Studies have shown that SiNPs can reduce sperm quantity and quality, increase abnormality rate, and damage testicular structure in male mice. SiNPs also affect protein levels and chromatin condensation, leading to compromised sperm quality and quantity.
ENVIRONMENTAL POLLUTION
(2021)
Article
Biochemistry & Molecular Biology
Galina M. Viryasova, Ekaterina A. Golenkina, Tibor Hianik, Nataliya V. Soshnikova, Nina G. Dolinnaya, Tatjana V. Gaponova, Yulia M. Romanova, Galina F. Sud'ina
Summary: The study reveals the impact of TLR9 and RAGE receptors on neutrophil functions in response to ODNs, particularly through modulating the 5-LOX pathway by binding with specific peptide sequences. Results demonstrate the potential biological activity of peptide-ODN complexes for use in vaccine adjuvants and antimicrobial therapeutics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yuri A. Piven, Margarita A. Yastrebova, Alvina Khamidullina, Alexander M. Scherbakov, Victor V. Tatarskiy, Julia A. Rusanova, Alexander Baranovsky, Veronica G. Zinovich, Tatyana S. Khlebnicova, Fedor A. Lakhvich
Summary: Novel O-acylated (E)-3-aryl-6,7-dihydrobenzisoxazol-4(5H)-one oximes were designed as potential HSP90 inhibitors. The synthesized compounds showed antiproliferative effects on breast cancer cell lines, especially HCC1954 cells. Compound 16s, with high antiproliferative potency, was selected for further evaluation and demonstrated the ability to induce apoptosis in cancer cells.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Svetlana I. Galkina, Natalia V. Fedorova, Alexander L. Ksenofontov, Ekaterina A. Golenkina, Marina V. Serebryakova, Vladimir I. Stadnichuk, Ludmila A. Baratova, Galina F. Sud'ina
Summary: Neutrophil adhesion to tissue, which is integrin-dependent, plays a role in inflammation. 4-Methylumbelliferone (4-MU), an inhibitor of hyaluronic acid synthesis, reduces neutrophil spreading and secretion, and selectively blocks adhesion-induced release of hydroxylysine. The anti-inflammatory effect of 4-MU may be associated with the suppression of secretory processes that ensure neutrophil invasion and initiate inflammation.
Article
Chemistry, Medicinal
Alexey S. Trenin, Elena B. Isakova, Michael Treshchalin, Vasilisa A. Polozkova, Elena P. Mirchink, Alexey A. Panov, Alexander Y. Simonov, Olga P. Bychkova, Victor V. Tatarskiy, Sergey N. Lavrenov
Summary: Three novel synthetic antibacterial agents containing tris(1H-indol-3-yl)methylium fragment were found to have high activity against bacteria in vitro and relatively low cytotoxicity against human cells. In vivo testing on mice showed good tolerance and relatively low toxicity.
Review
Biochemistry & Molecular Biology
Aseel Ali Hasan, Elena Kalinina, Victor Tatarskiy, Alexander Shtil
Summary: Oxidative stress refers to the excessive accumulation of reactive oxygen species (ROS), which are by-products of aerobic respiration, causing damage to macromolecules in cells. To prevent ROS accumulation, various antioxidant defense systems have evolved, with the Trx/TrxR system being the most important. The Trx/TrxR system is used to treat cancer by reducing target proteins and generating oxidized Trx. TrxR maintains reduced Trx levels using NADPH, thus maintaining cell homeostasis.
Editorial Material
Biochemistry & Molecular Biology
Galina F. Sud'ina
Review
Biochemistry & Molecular Biology
Aseel Ali Hasan, Victor Tatarskiy, Elena Kalinina
Summary: This article analyzes the effects of quercetin and curcumin in cancer treatment, highlighting their roles in regulating signaling pathways and influencing non-coding RNA. Additionally, the therapeutic potential of quercetin and curcumin in diabetes, obesity, inflammation, and bacterial infections is discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Leonid A. Ilchuk, Nina Stavskaya, Ekaterina A. Varlamova, Alvina Khamidullina, Victor V. Tatarskiy, Vladislav A. Mogila, Ksenia B. Kolbutova, Sergey A. Bogdan, Alexey M. Sheremetov, Alexandr N. Baulin, Irina A. Filatova, Yulia Yu Silaeva, Maxim A. Filatov, Alexandra Bruter
Summary: This article investigates the efficiency of different induction methods on knockout and finds that efficiency is correlated with the concentration of tamoxifen metabolites. Two specific cases of tamoxifen-induced knockout limitations are described. Increasing tamoxifen dosage and activation duration improves knockout rate in the brain but not in the uterus. Induction of knockout during embryonic development is impossible due to the negative effect of tamoxifen on gestation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Victor V. Tatarskiy, Olga V. Zakharova, Peter A. Baranchikov, Dmitry S. Muratov, Denis V. Kuznetsov, Alexander A. Gusev
Summary: 2D ultrathin nanomaterials have significant potential in biomedicine, but their cytotoxicity and interactions with other materials are not well understood. In this study, we investigated the effects of graphene oxide (GO) nanolayers and ZrS3 nanoribbons on colon carcinoma cells and found that ZrS3 exhibited strong cytotoxicity while GO reduced its toxicity. This finding demonstrates the ability of graphene oxide to decrease the cytotoxicity of other nanomaterials, which is important in the development of biocompatible nanocoatings and theranostic nanostructures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Pharmacology & Pharmacy
Alexey V. Sokolov, Boris V. Chernyak, Roman A. Zinovkin, Tsong-Long Hwang, Galina F. Sud'ina
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Galina F. F. Sud'ina, Ekaterina A. A. Golenkina, Anastasia S. S. Prikhodko, Natalia D. D. Kondratenko, Tatjana V. V. Gaponova, Boris V. V. Chernyak
Summary: This study demonstrates the involvement of mitochondrial reactive oxygen species in the activation of leukotriene synthesis in human neutrophils. Targeting mitochondria with antioxidants could be a promising strategy for inhibiting leukotriene synthesis and treating inflammatory pathologies.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Svetlana I. I. Galkina, Ekaterina A. A. Golenkina, Marina V. V. Serebryakova, Natalia V. V. Fedorova, Alexander L. L. Ksenofontov, Vladimir I. I. Stadnichuk, Galina F. F. Sud'ina
Summary: The study found that ivermectin has an inhibitory effect on neutrophil adhesion and secretion, possibly reducing cell invasion into tissue by suppressing the release of hydroxylysine, while promoting the release of phenylalanine and cathepsin G. Additionally, the experiment suggested the potential involvement of ivermectin in the inactivation of angiotensin II.
Review
Biochemistry & Molecular Biology
Leonid A. Ilchuk, Marina V. Kubekina, Yulia D. Okulova, Yulia Yu. Silaeva, Victor V. Tatarskiy, Maxim A. Filatov, Alexandra V. Bruter
Summary: The Mediator complex is a multi-subunit protein complex that regulates eukaryotic gene transcription by facilitating the interaction between transcriptional factors and RNA polymerase II. While most studies focus on simple models like tumor cell lines and yeast, transgenic mouse models are necessary to explore the role of Mediator components in physiological processes, disease, and development. Due to the embryonic lethality of constitutive knockout models, conditional knockout models and corresponding activator strains are required. Recent developments in genetic engineering techniques have made these models more accessible. This review discusses existing mouse models for studying the Mediator and the data obtained from corresponding experiments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Elena K. Kokinos, Sergey A. Tsymbal, Anastasia V. Galochkina, Svetlana A. Bezlepkina, Julia V. Nikolaeva, Sofia O. Vershinina, Anna A. Shtro, Victor V. Tatarskiy, Alexander A. Shtil, Eugenia V. Broude, Igor B. Roninson, Marina Dukhinova
Summary: Hyperactivation of the immune system is a life-threatening complication in viral and bacterial infections, especially pneumonia. This study found that a selective CDK8/19 inhibitor, Senexin B, could suppress the inflammatory response of monocytic cells to H1N1 influenza virus and bacterial lipopolysaccharides, reducing inflammation symptoms.
Article
Pharmacology & Pharmacy
Ekaterina A. Golenkina, Svetlana I. Galkina, Olga Pletjushkina, Boris Chernyak, Tatjana V. Gaponova, Yulia M. Romanova, Galina F. Sud'ina
Summary: This study demonstrates that Salmonella typhimurium can stimulate the production of LTB4 in neutrophils and suppress its transformation, leading to neutrophil swarming and elimination of pathogens during infection.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)