期刊
BMC MICROBIOLOGY
卷 16, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/s12866-016-0645-3
关键词
Surfactin; NRPS; Module deletion; Fusarium moniliforme
类别
资金
- National Natural Science Foundation of China [31271828]
- National Science and Technology Support program [2012BAK08807]
Background: Bacillus subtilis strain PB2-L1 produces the lipopeptide surfactin, a highly potent biosurfactant synthesized by a large multimodular nonribosomal peptide synthetase (NRPS). In the present study, the modules SrfA-A-Leu, SrfA-B-Asp, and SrfA-B-Leu from surfactin NRPS in B. subtilis BP2-L1 were successfully knocked-out using a temperature-sensitive plasmid, pKS2-mediated-based, homologous, recombination method. Results: Three novel surfactin products were produced, individually lacking amino acid Leu-3, Asp-5, or Leu-6. These surfactins were detected, isolated, and characterized by HPLC and LC-FTICR-MS/MS. In comparison with native surfactin, [Delta wLeu(3)] surfactin and [Delta Leu(6)] surfactin showed evidence of reduced toxicity, while [Delta Asp(5)] surfactin showed stronger inhibition than native surfactin against B. pumilus and Micrococcus luteus. These results showed that the minimum inhibitory concentration of [Delta Leu(6)] surfactin for Fusarium moniliforme was 50 mu g/mL, such that [Delta Leu(6)] surfactin could lead to mycelium projection, cell damage, and leakage of nucleic acids and protein. These factors all contributed to stimulating apoptosis in F. moniliforme. Conclusions: The present results revealed that [Delta Leu(6)] surfactin showed a significant antifungal activity against F. moniliforme and might successfully be employed to control fungal food contamination and improve food safety.
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