Review
Microbiology
Valerio M. Sora, Gabriele Meroni, Piera A. Martino, Alessio Soggiu, Luigi Bonizzi, Alfonso Zecconi
Summary: The One Health approach highlights the importance of antimicrobial resistance in both public health and food animal production, with specific focus on the ExPEC strains of E. coli which may transfer resistance genes to other pathogenic bacteria, posing a serious threat to public health globally.
Article
Infectious Diseases
Antonella Santona, Jose Joao Sumbana, Maura Fiamma, Massimo Deligios, Elisa Taviani, Samuel Elija Simbine, Tomas Zimba, Jahit Sacarlal, Salvatore Rubino, Bianca Paglietti
Summary: ESBL-producing ExPEC, including high-risk lineages, were identified in extraintestinal infections at Maputo Central Hospital. The majority of isolates showed multidrug resistance, with ESBL genes associated with resistance to cefotaxime and ceftazidime. Furthermore, different sequence types were found to carry CTX-M transposition units, potentially facilitating the dissemination of ESBL genes. Genomic analysis revealed a diverse array of virulence-associated genes and clonality among isolates, suggesting the presence of hospital-acquired infections and long-term persistence.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Food Science & Technology
Lucas Harrison, Gregory H. Tyson, Errol Strain, Rebecca L. Lindsey, Nancy Strockbine, Olgica Ceric, Gamola Z. Fortenberry, Beth Harris, Sheryl Shaw, Glenn Tillman, Shaohua Zhao, Uday Dessai
Summary: This study used genomic data to assess potential sources of ExPEC strains and found a higher likelihood of human infection-related strains in food animals and companion animals. Additionally, strains in high relative risk groups were more commonly resistant to antimicrobial drugs. This study highlights the importance of a One Health approach in identifying and managing human pathogens.
Article
Microbiology
Andre Becker S. Saidenberg, Arnoud H. M. van Vliet, Marc Stegger, Thor Bech Johannesen, Torsten Semmler, Marcos Cunha, Alessandro C. de O. Silveira, Eleine Kuroki Anzai, Isabel C. A. Scaletsky, Anders Dalsgaard, Roberto M. La Ragione, Terezinha Knobl
Summary: Extraintestinal pathogenic Escherichia coli (ExPEC) is a globally distributed pathogen associated with human and companion animal diseases, while avian pathogenic pathotype (APEC) severely impacts poultry health and production. The similarities between APEC and human ExPEC suggest a potential for zoonotic transmission.
VETERINARY MICROBIOLOGY
(2022)
Review
Immunology
Marc Bonten, James R. Johnson, Anita H. J. van den Biggelaar, Leonidas Georgalis, Jeroen Geurtsen, Patricia Ibarra de Palacios, Stefan Gravenstein, Thomas Verstraeten, Peter Hermans, Jan T. Poolman
Summary: The study revealed a significant burden of E. coli bacteremia in older adults in high-income countries, especially in individuals aged 55 and above. Implementing community-level prevention programs can effectively prevent E. coli bacteremia and urinary tract infections in this age group.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Pharmacology & Pharmacy
Zermina Zeb, Sadiq Azam, Noor Rehman, Ibrar Khan, Sumera Afzal, Gul-E-Sehra, Anhar Ullah, Muhammad Absar
Summary: The study investigated 367 ExPEC isolates from patients in Pakistan, revealing a high prevalence of hemolysin positivity, multi-drug resistance, and presence of virulence genes, with sat being the most prevalent. The highest resistance was observed towards cefotaxime, ampicillin, and aztreonem. Overall, 15 different virulence gene combinations and 16 virotypes were identified.
PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Immunology
Eveline Weerdenburg, Todd Davies, Brian Morrow, Aldert L. Zomer, Peter Hermans, Oscar Go, Bart Spiessens, Thijs van den Hoven, Gunter van Geet, Moussa Aitabi, Chitrita DebRoy, Edward G. Dudley, Marc Bonten, Jan Poolman, Jeroen Geurtsen
Summary: Extraintestinal pathogenic Escherichia coli (ExPEC) is a major cause of bacteremia worldwide, and the dominant O serotype among ExPEC bacteremia isolates is O25B, particularly among multidrug-resistant isolates.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Microbiology
Ana Carolina M. Santos, Jose F. Santos-Neto, Liana O. Trovao, Ricardo F. T. Romano, Rosa Maria Silva, Tania A. T. Gomes
Summary: Extraintestinal pathogenic Escherichia coli (ExPEC) is the leading cause of urinary tract and bloodstream infections worldwide. This study assessed the pathogenic potential of E. coli strains lacking common ExPEC virulence factors and found that some of these strains displayed characteristics of pathogens. These findings are important for understanding the pathogenesis of ExPEC and developing treatment strategies.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Emma Bartram, Masanori Asai, Philippe Gabant, Sivaramesh Wigneshweraraj
Summary: This study retrofitted probiotic Escherichia coli bacteria to overproduce heterologous bacteriocins and found that strains producing the lowest amount of bacteriocins displayed the highest antibacterial efficacy with minimal impact on their fitness, highlighting important considerations for retrofitting probiotic bacteria.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Review
Infectious Diseases
Juraj Bosak, Matej Hrala, Lenka Micenkova, David Smajs
Summary: Bacteriocins, peptides or proteins with relatively narrow spectra of antibacterial activities produced by various bacterial species, represent a potential alternative to antibiotic treatment. They have potential applications in human and animal medicine, as well as in the food industry.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2021)
Review
Biochemistry & Molecular Biology
Babak Pakbin, Wolfram M. Bruck, John W. A. Rossen
Summary: Escherichia coli are versatile microorganisms found in the normal intestinal microbiota of humans and animals. They can become emerging human pathogens by acquiring genes encoding virulence factors. Nine enteric E. coli pathotypes have been characterized, utilizing various virulence factors to cause intestinal and extraintestinal diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
D. M. Verboom, M. R. J. Varkila, B. Morrow, T. Davies, P. Ibarra de Palacios, J. Poolman, P. W. M. Hermans, E. G. Dudley, E. Roberts, O. L. Cremer, M. J. M. Bonten
Summary: This study investigated E. coli BSI in ICU patients in the Netherlands and identified common O-serogroups. The research suggests that a vaccine currently in development may help prevent E. coli BSI.
Article
Biology
Jolanta Sarowska, Tomasz Olszak, Agnieszka Jama-Kmiecik, Magdalena Frej-Madrzak, Bozena Futoma-Koloch, Andrzej Gawel, Zuzanna Drulis-Kawa, Irena Choroszy-Krol
Summary: This study analyzed the phylogenetic background, virulence patterns, and drug susceptibility of 132 E. coli isolates and correlated these features with the source of bacterial isolation. The results showed similar virulence patterns between isolates from poultry farms and food, while the isolates from patients with UTI symptoms showed diverse virulence patterns. In terms of drug resistance, the three groups of isolates showed similarity.
Article
Microbiology
Maria Belen Sanz, Denise De Belder, J. M. de Mendieta, Diego Faccone, Tomas Poklepovich, Celeste Lucero, Melina Rapoport, Josefina Campos, Ezequiel Tuduri, Mathew O. O. Saavedra, Claudia van der Ploeg, Ariel Roge, Carbapenemases-ExPEC Grp, Fernando Pasteran, Alejandra Corso, Adriana E. E. Rosato, Sonia A. A. Gomez
Summary: This study investigated the distribution and molecular epidemiology of carbapenemase-producing ExPEC in Argentina. The study found a high clonal diversity with CC10 and CC/ST131 being the most common clones. Other international clones were also identified. Further studies are needed to determine the plasmid families responsible for spreading carbapenemases in this region.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Mykhailo Savin, Gabriele Bierbaum, Judith Kreyenschmidt, Ricarda Maria Schmithausen, Esther Sib, Silvia Schmoger, Annemarie Kaesbohrer, Jens Andre Hammerl
Summary: Escherichia coli with high multidrug resistance rates and extraintestinal pathogenic potential were found in poultry and pig slaughterhouses in Germany. The isolates were reservoirs for numerous antimicrobial resistance determinants and contained high-risk clones associated with human infections worldwide. The presence of uropathogenic E. coli in the effluent of a poultry slaughterhouse's wastewater treatment plant suggests potential dissemination into surface waters.
Article
Microbiology
Juraj Bosak, Lenka Micenkova, Magda Dolezalova, David Smajs
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2016)
Article
Microbiology
Lenka Micenkova, Alzbeta Benova, Lucia Frankovicova, Juraj Bosak, Martin Vrba, Alena Sevcikova, Marta Kmet'ova, David Smajs
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2017)
Article
Microbiology
Lenka Micenkova, Lucia Frankovicova, Iva Jabornikova, Juraj Bosak, Petr Dite, Jan Smarda, Martin Vrba, Alena Sevcikova, Marta Kmet'ova, David Smajs
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY
(2018)
Article
Veterinary Sciences
Linda Grillova, Ivo Sedlacek, Gabriela Pachnikova, Eva Stankova, Pavel Svec, Pavla Holochova, Lenka Micenkova, Juraj Bosak, Iva Slaninova, David Smajs
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2018)
Article
Microbiology
Ivana Jamborova, Brian D. Johnston, Ivo Papousek, Katerina Kachlikova, Lenka Micenkova, Connie Clabots, Anna Skalova, Katerina Chudejova, Monika Dolejska, Ivan Literak, James R. Johnson
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2018)
Article
Multidisciplinary Sciences
Juraj Bosak, Lenka Micenkova, Matej Hrala, Katarina Pomorska, Michaela Kunova Bosakova, Pavel Krejci, Eduard Gopfert, Martin Faldyna, David Smajs
SCIENTIFIC REPORTS
(2018)
Article
Biotechnology & Applied Microbiology
Zuzana Jirsova, Marie Heczkova, Helena Dankova, Hana Malinska, Petra Videnska, Hana Vespalcova, Lenka Micenkova, Lenka Bartonova, Eva Sticova, Alena Lodererova, Lucia Prefertusova, Alena Sekerkova, Jaromir Hradecky, Monika Cahova
BIOMED RESEARCH INTERNATIONAL
(2019)
Article
Microbiology
Juraj Bosak, Matej Hrala, Viktoria Pirkova, Lenka Micenkova, Alois Cizek, Jiri Smola, Dana Kucerova, Zdenka Vackova, Eva Budinska, Ivana Kolackova, David Smajs
VETERINARY MICROBIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Lenka Micenkova, Juraj Bosak, Stanislav Smatana, Adam Novotny, Eva Budinska, David Smajs
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2020)
Article
Multidisciplinary Sciences
Lenka Micenkova, Juraj Bosak, Jiri Kucera, Matej Hrala, Tereza Dolejsova, Ondrej Sedo, Dirk Linke, Radovan Fiser, David Smajs
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Petra Videnska, Kristyna Smerkova, Barbora Zwinsova, Vlad Popovici, Lenka Micenkova, Karel Sedlar, Eva Budinska
SCIENTIFIC REPORTS
(2019)
Review
Infectious Diseases
Juraj Bosak, Matej Hrala, Lenka Micenkova, David Smajs
Summary: Bacteriocins, peptides or proteins with relatively narrow spectra of antibacterial activities produced by various bacterial species, represent a potential alternative to antibiotic treatment. They have potential applications in human and animal medicine, as well as in the food industry.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2021)
Article
Microbiology
Pavel Svec, Marcel Kosina, Michal Zeman, Pavla Holochova, Stanislava Kralova, Eva Nemcova, Lenka Micenkova, Urvashi, Vipin Gupta, Utkarsh Sood, Rup Lal, Suresh Korpole, Ivo Sedlacek
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2020)
Article
Microbiology
Ivo Sedlacek, Roman Pantucek, Michal Zeman, Pavla Holochova, Ondrej Sedo, Eva Stankova, Pavel Svec, Stanislava Kralova, Petra Videnska, Lenka Micenkova, Urvashi, Suresh Korpole, Rup Lal
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2020)