Article
Immunology
Peng Chen, Mingming Zhang, Yichan Zhang, Jun Li, Xihe Wan, Tingli Lv, Yiyue Chen, Zhigang Zhao, Zhihao Ma, Zhu Zhu, Lihua Chen, Zhen Li, Zisheng Wang, Guo Qiao
Summary: This study analyzed the impact of Cyprinid herpesvirus 2 (CyHV-2) infection on the gut microbiota and metabolite composition of gibel carp. The results showed that CyHV-2 infection significantly altered the gut microbiota and metabolite profiles, leading to an increase in the opportunistic pathogen Aeromonas. Furthermore, modulation of tryptophan metabolism affected the abundance of Aeromonas, the transcription of antiviral cytokines, and the infection level of CyHV-2.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Zhao-Ran Dong, Qing-Jiang Mu, Wei-Guang Kong, Da-Cheng Qin, Yong Zhou, Xin-You Wang, Gao-Feng Cheng, Yang-Zhi Luo, Tao-Shan Ai, Zhen Xu
Summary: This study developed an oral vaccine against CyHV-2 using yeast cell surface display technology and demonstrated its protective efficacy in gibel carp. The vaccine group showed higher activities of immune-related enzymes and gene expression, and strong innate and adaptive immune responses were elicited in mucosal and systemic tissues after oral vaccination with the yeast vaccine.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Meizhen Su, Cuiyu Lu, Ruizhe Tang, Xiaomi Zhang, Liqun Lu
Summary: The study demonstrates that the plant alkaloid berberine can effectively inhibit the expression and replication of CyHV-2 in crucian carp through negative regulation of the NF-xB signaling pathway. This indicates that berberine has the potential to serve as a small molecular drug for controlling CyHV-2 infection in fish. The findings also show that berberine can attenuate inflammatory injury and viral load in CyHV-2-infected crucian carp cell lines in a dose-dependent manner.
Article
Pharmacology & Pharmacy
Meizhen Su, Ruizhe Tang, Hao Wang, Liqun Lu
Summary: Berberine hydrochloride (BBH) effectively inhibits the replication of Cyprinid herpesvirus 2 (CyHV-2) and provides protection for Crucian carps, showing favorable pharmacokinetic properties in vivo.
ANTIVIRAL RESEARCH
(2021)
Article
Fisheries
Guo Qiao, Yichan Zhang, Jiwu Zhang, Wei Chen, Chang Wei, Peng Chen, Wenping Yang, Zhu Zhu, Mingming Zhang, Zhigang Zhao, Xihe Wan
Summary: Supplementation of poly-beta-hydroxybutyrate (PHB) can enhance the disease resistance and antioxidant capacity of gibel carps against CyHV-2 infection, inhibit viral proliferation in the gill, and improve the immune response. These findings are of great significance for developing novel prevention strategies to control CyHV-2 infection.
AQUACULTURE RESEARCH
(2023)
Article
Immunology
Tingting Zhang, Yuchao Gu, Xiaohan Liu, Rui Yuan, Yang Zhou, Yaping Dai, Ping Fang, Yongjie Feng, Guangli Cao, Hui Chen, Renyu Xue, Xiaolong Hu, Chengliang Gong
Summary: Encapsulating vaccines into protein microcrystals for stable delivery is a promising approach, as demonstrated by this study focusing on vaccinating gibel carp against CyHV-2 using polyhedra. The results showed that the vaccine proteins encapsulated in polyhedra could be protected from degradation, and vaccinated fish exhibited increased antibody titers and survival rates following CyHV-2 challenge, suggesting a novel strategy for developing aquaculture microencapsulated vaccines.
Article
Microbiology
Jinwei Gao, Yiwen Hu, Min Xie, Hao Wu, Jiayu Wu, Bingwen Xi, Rui Song, Dongsheng Ou
Summary: Infection of gibel carp with Cyprinid herpesvirus II leads to disruptions in physiological metabolic processes, liver and kidney damage, and different spatiotemporal expression patterns of Toll-like receptor 2 and Toll-like receptor 9, ultimately stimulating an antiviral response.
Article
Fisheries
Chang Wei, Chaonan Xu, Yuyu Sun, Jinzhou Li, Motohiko Sano, Qiang Li
Summary: This study found that CyHV-2 can establish persistent or latent infection in asymptomatic farmed gibel carp, and different viral mRNA expression patterns were observed in different individuals. This indicates that apparently healthy gibel carp can also be a source of infection.
Article
Fisheries
Arathi Dharmaratnam, Arun Sudhagar, Thangaraj Raja Swaminathan
Summary: In this experiment, a heat-inactivated CyHV-2 vaccine was developed and evaluated in goldfish. The vaccine showed significant upregulation of immune genes and provided better protection against CyHV-2 in the challenged fish.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Immunology
Arathi Dharmaratnam, Arun Sudhagar, Sundar Raj Nithianantham, Sweta Das, Thangaraj Raja Swaminathan
Summary: In this study, the potential of four different reference genes in seven different tissues of goldfish was examined. EF1AA was identified as the best gene in healthy fish, while 18S was found to be stable in healthy fish but least expressed in challenged fish. ACTB also showed stable expression in both healthy and challenged fish. The results emphasize the importance of thorough study of reference genes for accurate gene expression analysis in different tissues.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2021)
Article
Fisheries
Bing-Yao Sun, Hai-Yan Kou, Pan -Yang Jian, Ling-Jiao Kong, Jie Fang, Peng-Kun Meng, Kang Wu, Cai-Gen Yang, Gang Yang, Xue-Hong Song
Summary: This study aimed to develop a specific IgY against CyHV-2 infection in gibel carp. Specific IgY was generated by immunizing laying hens with a recombinant D4ORFs protein and processed into spray-dried egg powder. The results showed that gibel carp receiving D4ORFs IgY had higher survival rates and lower viral load, as well as improved health and enhanced antiviral defense.
Article
Immunology
Arathi Dharmaratnam, Arun Sudhagar, Sweta Das, Reshma Rajeev Nair, Sundar Raj Nithianantham, Prasannan Geetha Preena, Narendrakumar Lekshmi, Thangaraj Raja Swaminathan
Summary: This study focused on developing an inactivated vaccine for the goldfish hematopoietic necrosis viral disease (GHNVD) and examining its immunogenicity. The results showed that the formalin-inactivated vaccine triggered immune gene expression in goldfish and significantly improved survival rates in a virus challenge experiment.
MICROBIAL PATHOGENESIS
(2022)
Article
Fisheries
Hong-jun Wu, Fan Liu, Hong-Yao Du, Yan-Ge He, Chuan-He Bao, Zhong Wei, Ruo-lin Zhu
Summary: A Carassius carassius gill cell line (CCG) was constructed and successfully passaged up to 90 passages, providing a useful tool for studying the propagation and pathogenesis of CyHV-2.
AQUACULTURE REPORTS
(2022)
Article
Agriculture, Dairy & Animal Science
Wanjie Cai, Lele Fu, Cui Liu, Linyue He, Haokun Liu, Dong Han, Xiaoming Zhu, Yunxia Yang, Junyan Jin, Shouqi Xie
Summary: This study investigated the effect of D-ribose on the flesh quality of gibel carp. The results showed that D-ribose supplementation improved the nutritional value, texture, and flavor of the fish. It enhanced the water-holding capacity and hardness of the flesh, facilitated the deposition of amino acids and fatty acids, and activated purine metabolism pathways.
Article
Fisheries
Lu Yu, Qikang Chen, Xin Chu, Yang Luo, Zizhao Feng, Liqun Lu, Ye Zhang, Dan Xu
Summary: In this study, miR-124 was identified as a negative regulator of ccBAX gene expression during CyHV-2 infection in silver crucian carp. The results showed that miR-124 significantly down-regulated the expression of the ccBAX gene in silver crucian carp, indicating a potential mechanism of regulation during viral infection.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Hunter S. Bailey, Ashley N. Fincannon, Lee A. Fuiman
Summary: This study investigated the transfer of fatty acids from broodstock diets to eggs and the time needed for the eggs to equilibrate to the diet in Southern flounder. The findings indicate that different diet treatments significantly affect the fatty acid composition of eggs, and it takes 8-16 weeks for the eggs to adapt to changes in the adult diet. These results are important for improving broodstock diets and feeding protocols in stock-enhancement programs.
Article
Fisheries
Yu Liu, Mingtao Lei, Hector Victor, Yan Wang
Summary: This study confirms the feasibility of replacing raw fish diet with a formulated diet in commercial farming of large yellow croaker, which can increase fish survival and yield, and reduce feed cost and body lipid deposition.
Article
Fisheries
Xiaoyan Zhang, Lize San, Yucong Yang, Yuehong Tao, Jiangong Ren, Yufeng Liu, Zhongwei He, Jiashuo Di, Ziteng Pei, Guixing Wang, Jilun Hou
Summary: Gynogenesis is an effective technique for establishing homogeneous lines and confirming potential chromosomal mechanisms of sex determination in fish. This study successfully induced gynogenetic families in Thamnaconus modestus and established a complete method for gynogenesis induction.
Article
Fisheries
Julieta C. Martinelli, Megan Considine, Helen R. Casendino, Carolyn M. Tarpey, Isadora Jimenez-Hidalgo, Jacqueline L. Padilla-Gamino, Teri L. King, Lorenz Hauser, Steven Rumrill, Chelsea L. Wood
Summary: Shell-boring polychaetes have caused significant damage to mariculture operations worldwide, particularly in the US Pacific region. This study provides a comprehensive dataset on the prevalence, species distribution, and environmental associations of these parasites, highlighting the impact of season, culturing methods, and environmental factors on infestation.
Article
Fisheries
Juan Gao, Xueshan Li, Kangle Lu, Kai Song, Jian Zhang, Ling Wang, Chunxiao Zhang
Summary: This study investigated the effects of dietary protein levels on growth performance, antioxidant capacity, digestive enzyme activity, and metamorphosis rate of bullfrog tadpoles. The optimal dietary protein level for bullfrog tadpoles was estimated to be 42.49%-46.50% of the diet.
Article
Fisheries
Qiang Li, Lijun Duan, Dongsheng Jin, Yuxin Chen, Yirong Lou, Qianjin Zhou, Zhongjie Xu, Fangjie Chen, Hongxian Chen, Guizong Xu, Maocang Yan, Guanjun Yang, Jianfei Lu, Yanjun Zhang, Jiong Chen
Summary: This study developed a centrifugal microfluidic chip with on-chip RPA to detect five pathogenic microorganisms. The chip enabled the parallel analysis of six genetic markers from a single sample and allowed for the highly automated detection of multiple samples. Compared with PCR and DNA sequencing, the on-chip RPA assay showed high sensitivity and specificity for detecting multiple pathogens in shrimp aquaculture.
Article
Fisheries
Xingchen Huo, Pengxu Wang, Fengxia Zhao, Qian Liu, Qingqing Tian, Lingjie Tang, Maolin Lv, Zhaohui Wei, Chunrong Yang, Jianguo Su
Summary: Bacterial diseases in aquaculture ponds have negative effects on sustainable development. The use of composite I20 biotherapy has been shown to effectively treat bacterial diseases in bullfrog ponds, providing a new strategy for controlling bacterial diseases in aquaculture.
Article
Fisheries
Peng Yin, Takaya Saito, Per Gunnar Fjelldal, Bjorn Tharandur Bjornsson, Sofie Charlotte Remo, Sandeep Sharma, Rolf Erik Olsen, Kristin Hamre
Summary: This study aims to investigate the effect of seasonal changes in temperature and photoperiod on the redox metabolism of Atlantic salmon at the molecular level. The research findings suggest that the changing photoperiod and temperature influenced the redox metabolism of Atlantic salmon, highlighting the importance of oxidative status in the ecological implications.
Article
Fisheries
Nicholas Oppong Mensah, Jeffery Kofi Asare, Emmanuel Tetteh-Doku Mensah, Ernest Christlieb Amrago, Frank Osei Tutu, Anthony Donkor
Summary: This study investigates aquaculture farmers' preference for climate-smart aquaculture insurance products, the challenges they face, and their preferred insurance coverage. The results show that farmers prefer Climate-Induced Aquaculture Stock Mortality Insurance and the most significant constraint is the delay in claim settlement.
Article
Fisheries
Qiu-Ping Chai, Pei Wu, Wei-Dan Jiang, Yang Liu, Hong-Mei Ren, Xiao-Wan Jin, Lin Feng, Xiao-Qiu Zhou
Summary: The study found that appropriate levels of potassium diformate (KDF) can enhance the immune defense and mediate the inflammatory process in fish, possibly through the regulation of T cell differentiation via JAK-STAT and NF-kappa B signaling pathways.
Article
Fisheries
Tian Zhu, Haomin Jia, Haopeng Zhang, Yujing Xiao, Cui Han, Jiaxin Yang
Summary: Chicken manure has significant effects on the cultivation of Chlorella and rotifers. The optimal amount of chicken manure extract for continuous cultivation is 10 mLL-1. Chicken manure can promote the growth of Chlorella and improve the fatty acid composition, indirectly enhancing the growth condition and fatty acid content of rotifers.
Article
Fisheries
Zheng Luo, Yang Yu, Zhenning Bao, Fuhua Li
Summary: This study analyzed the heritability and genetic correlation of two growth traits in Pacific white shrimp and evaluated the genomic prediction using different genomic selection models. The results showed that the NeuralNet model had the highest prediction accuracy and better prospects for predicting shrimp growth traits.
Article
Fisheries
Alberto Ruiz, Ignasi Sanahuja, Karl B. Andree, Dolors Furones, Paul G. Holhorea, Josep A. Calduch-Giner, Jose J. Pastor, Marc Vinas, Jaume Perez-Sanchez, Sofia Morais, Enric Gisbert
Summary: Supplementing diets with a combination of spice oleoresins can reduce lipid accumulation and inflammation in farmed fish. The combination of spice oleoresins also has an immunomodulatory effect on the fish's intestinal immune system, potentially offering anti-inflammatory benefits.
Article
Fisheries
Beibei Zhao, Renhui Mei, Le Li, Di Hu, Lei Li
Summary: This study reveals that JfCXCL8_L1a and JfCXCL8_L1b have different immune pathways, and JfCXCL8_L1b plays a significant role in enhancing the adaptive immunity of T cell-dependent antigen.
Article
Fisheries
Yuhang Liu, Danying Cao, Nan Wu, Xuyang Zhao, Qingsong Zhu, Lian Su, Fatima Altaf, Qianqian Zhang, Haokun Liu, Yongming Li, Bruno Hamish Unger, Yingyin Cheng, Wanting Zhang, Aihua Li, Yaping Wang, Xiao-Qin Xia
Summary: Based on previous research, sinomenine has been found to protect mucosal immunity in farmed fish species by preventing intestinal pathological changes and regulating gene expression related to inflammation. It also enhances immune homeostasis and controls the growth of pathogenic bacteria.