Review
Biotechnology & Applied Microbiology
Angelo B. Banares, Grace M. Nisola, Kris Nino G. Valdehuesa, Won-Keun Lee, Wook-Jin Chung
Summary: This review discusses the causes of D-xylonic acid accumulation in metabolically engineered XOP-utilizing microorganisms, and how to address this issue through different engineering and synthetic biology techniques, with a focus on bacterial strains. Additionally, the ability of certain microorganisms to produce and tolerate D-xylonic acid is also examined as an important aspect in developing efficient microbial cell factories.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Wenting Gao, Ying Yin, Pan Wang, Wei Tan, Mingliang He, Jianping Wen
Summary: In this study, the genes of the Dahms xylose-utilization pathway were integrated into Bacillus subtilis and a metabolic control module was designed, resulting in an increased yield of fengycin. Metabonomic analysis revealed the underlying factors for the increase of fengycin titer.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Energy & Fuels
Dongnv Jin, Jiliang Ma, Yancong Li, Gaojie Jiao, Kangning Liu, Shaolong Sun, Jinghui Zhou, Runcang Sun
Summary: Biorefining is a beneficial method for generating high-value biofuels or chemicals from biomass-derived feedstocks. Xylonic acid, an essential chemical produced by biorefining from xylose, has various applications and market prospects. The current production methods of xylonic acid include chemical and biological approaches, with the separation of xylose being a critical step.
Article
Agricultural Engineering
Zhen Zhu, Rong Huang, Shuangquan Yao, Yiruo Liu, Qibo Zhang, Xin Zhou, Kankan Jiang
Summary: In this study, sugarcane bagasse was pretreated using a renewable organic acid (xylonic acid) derived from the biooxidation of xylose. The effects of reaction temperature and time on the release of fermentable xylose and glucose were investigated. After optimization, a pretreatment with 1% xylonic acid at 190 degrees C for 30 min was selected, resulting in a release of 70% xylose and an enzymatic hydrolysis yield of 86.5%. The pretreated hydrolysate liquor was also shown to be suitable for xylonate production through the biooxidation of Gluconobacter oxydans. Additionally, the sequential process of xylonic acid pretreatment and saccharification provides a pathway for recycling lignin as value-added bioproducts. Overall, this study presents a green-like strategy for fully utilizing sugarcane bagasse.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Roland S. Kun, Sandra Garrigues, Marcos Di Falco, Adrian Tsang, Ronald P. de Vries
Summary: In this study, chimeric GaaR-XlnR transcription factors were generated using the CRISPR/Cas9 system in Aspergillus niger, with inducibility by D-xylose confirmed. Proteomics analysis showed that Delta gaaR mutants carrying the chimeric transcription factor produced several pectinolytic enzymes, leading to improved degradation efficiency of pectin.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Dan Xiao, Xianzhi Xu, Kaiyu Gao, Mengjiao Wang, Wen Zhang, Chuanjuan Lu, Xia Wang, Qian Wang, Ping Xu, Cuiqing Ma, Chao Gao
Summary: A FRET-based biosensor for detecting D-2-Hydroxyglutarate (D-2-HG) was developed, which showed excellent sensitivity and specificity. It can accurately measure the concentration of D-2-HG in body fluids and cell-related samples in vitro.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biophysics
Dan Xiao, Chunxia Hu, Xianzhi Xu, Chuanjuan Lue, Qian Wang, Wen Zhang, Chao Gao, Ping Xu, Xia Wang, Cuiqing Ma
Summary: A biosensor capable of detecting both D-lactate and L-lactate was developed in this study, using an allosteric transcription factor LldR and amplified luminescent proximity homogeneous assay technology. The optimized biosensor, BLac-6, showed high accuracy in lactate concentration detection and was tentatively applied in industrial fermentation.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemical Research Methods
Jiawei Li, Mario Roque Huanca Nina, Xiaoyan Zhang, Yunpeng Bai
Summary: Researchers have developed two novel variants of a transcription factor that can detect PA and TPA and activate the expression of a fluorescent protein. Using these variants, they constructed whole-cell biosensors that can detect as little as 10 μM of PA or TPA. They also engineered a PAE hydrolase to enhance its activity towards DBP and p-nitrophenyl butyrate.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Cell Biology
Lanlan Liu, Xiaoli Zhang, Ruihong Zhang, Liyan Wang, Sujuan Zhi, Xiaoning Feng, Xuyue Liu, Ying Shen, Jing Hao
Summary: This study found that Sohlh2 can accelerate pulmonary fibrosis and induce cell death through enhancing oxidative stress. p62 is a direct target of Sohlh2 and mediates the effects of Sohlh2 on ROS generation and cell apoptosis.
CELL DEATH & DISEASE
(2023)
Article
Biochemical Research Methods
Cynthia Ni, Kevin J. Fox, Kristala L. J. Prather
Summary: Microbes are able to facilitate the production of valuable chemicals more sustainably by utilizing galacturonate as both inducer and substrate, eliminating the need for an extrinsic chemical inducer. The activation of pathway expression in response to the feed is actuated by a galacturonate-responsive transcription factor biosensor, showing a favorable biosensor response in the presence and absence of galacturonate consumption. The productivity via substrate-induction with this control strategy outperformed traditional chemical inducers and constitutive expression controls for producing d-glyceric acid.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Biochemical Research Methods
Ingrida Kutraite, Naglis Malys
Summary: This study reports the characterization of a transcription factor-based inducible system that responds to gallic acid in a dose-dependent manner. The activation of this system requires gallate dioxygenase activity and the effector molecule is identified as an intermediate of gallic acid metabolism. Furthermore, the applicability of the P. putida-based biosensor is demonstrated by detecting and measuring gallic acid in tea leaf extracts.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Boran Ni, Yi Liu, Xue Gao, Mengru Cai, Jing Fu, Xingbin Yin, Jian Ni, Xiaoxv Dong
Summary: This study demonstrated that ISL can protect cells from EMO-induced liver injury, which may be associated with the activation of Nrf2, reduction of ROS generation, and enhancement of detoxification enzyme expression.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2022)
Article
Biochemical Research Methods
Jonathan Tellechea-Luzardo, Hector Martin Lazaro, Raul Moreno Lopez, Pablo Carbonell
Summary: Allosteric transcription factor (aTF) based biosensors are widely used in engineering genetic circuits. However, the scattered and incomplete knowledge about validated molecule-TF pairs and the limited number of known TF-compound interactions pose challenges in finding new TF-ligand pairs. In this study, we present Sensbio, a computational tool that utilizes a TF-ligand reference database to identify potential transcription factors activated by specific input molecules. Our tool includes algorithms, an online application, and a predictive model based on machine learning for discovering new matches.
BMC BIOINFORMATICS
(2023)
Article
Biochemistry & Molecular Biology
Kun Wang, Xujie Cui, Xiaocui Ling, Jiarui Chen, Jiachen Zheng, Yuling Xiang, Weihui Li
Summary: D-xylose acts as a signaling molecule to regulate lipid metabolism and affect physiological characteristics in mycobacteria.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Shuling Zhang, Lijun Dong, Xue Zhang, Xiaohong Fu, Lin Zhao, Lizhu Wu, Xingfen Wang, Jianfeng Liu
Summary: In cotton, the transcription factor GhWRKY70 is up-regulated by Verticillium dahliae and plays a positive role in Verticillium wilt tolerance by promoting the production of jasmonic acid (JA) through regulation of GhAOS1 expression.
Article
Biotechnology & Applied Microbiology
Kristine Rose M. Ramos, Kris Nino G. Valdehuesa, Angelo B. Banares, Grace M. Nisola, Won-Keun Lee, Wook-Jin Chung
BIOTECHNOLOGY LETTERS
(2020)
Review
Green & Sustainable Science & Technology
Teklebrahan G. Weldemhret, Angelo B. Banares, Kristine Rose M. Ramos, Won-Keun Lee, Grace M. Nisola, Kris Nino G. Valdehuesa, Wook-Jin Chung
Article
Environmental Sciences
Hana G. Zeweldi, Anelyn P. Bendoy, Myoung Jun Park, Ho Kyong Shon, Eldin M. Johnson, Han-Seung Kim, Hern Kim, Wook-Jin Chung, Grace M. Nisola
Summary: A phosphonium-based ionic liquid with lower critical solution temperature has been successfully synthesized and evaluated as a reusable draw solution for forward osmosis. It shows promising performance in treating saline feed, and can be conveniently regenerated and reused with consistent FO performance. The cost estimates reveal the potential of combining FO with thermo-cyclic regeneration for desalination applications.
Article
Biotechnology & Applied Microbiology
Rhudith B. Cabulong, Angelo B. Banares, Grace M. Nisola, Won-Keun Lee, Wook-Jin Chung
Summary: A modified microbial strain capable of producing glycolic acid from d-xylose was engineered to utilize cellobiose as a co-substrate, resulting in improved glycolic acid production with high yield.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Review
Biotechnology & Applied Microbiology
Angelo B. Banares, Grace M. Nisola, Kris N. G. Valdehuesa, Won-Keun Lee, Wook-Jin Chung
Summary: The lignocellulosic sugar d-xylose is gaining attention as a low-cost alternative substrate for genetically engineered organisms. Escherichia coli is widely used as the host organism for engineered microbial cell factories, with a deep understanding of its systems. Limited literature reviews focus on using engineered E. coli for converting d-xylose to target compounds, making this review essential for researchers in this field.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Angelo B. Banares, Grace M. Nisola, Kris Nino G. Valdehuesa, Won-Keun Lee, Wook-Jin Chung
Summary: This review discusses the causes of D-xylonic acid accumulation in metabolically engineered XOP-utilizing microorganisms, and how to address this issue through different engineering and synthetic biology techniques, with a focus on bacterial strains. Additionally, the ability of certain microorganisms to produce and tolerate D-xylonic acid is also examined as an important aspect in developing efficient microbial cell factories.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Environmental Sciences
John Edward L. Sio, Erwin C. Escobar, Hern Kim, Wook-Jin Chung, Grace M. Nisola
Summary: A magnetic adsorbent HPCA@SiO2@Fe3O4 with high HPCA loading and good stability was developed for Co (II) recovery from liquid waste.
Article
Materials Science, Multidisciplinary
Lawrence A. Limjuco, Hiluf T. Fissaha, Hern Kim, Grace M. Nisola, Wook-Jin Chung
ACS APPLIED POLYMER MATERIALS
(2020)
