Article
Food Science & Technology
Yunhe Zhang, Bo Shan, Jiashun Gong, Yongjin Hu
Summary: This study focuses on the regulation of biogenic amine synthesis in Sanchuan ham by quorum sensing. It found that high temperature and low salt conditions promote the production of tyramine by Enterococcus faecium, a strain isolated from Sanchuan ham. It was also discovered that alanyl-leucine acts as a quorum sensing signal molecule, activating the expression of tyrosine decarboxylase and regulating the synthesis of tyramine. These findings provide a theoretical basis for reducing biogenic amine accumulation in ham.
FOOD SCIENCE & NUTRITION
(2022)
Article
Food Science & Technology
Seung-Eun Oh, Sojeong Heo, Gawon Lee, Hee-Jung Park, Do-Won Jeong
Summary: In this study, the Enterococcus faecium strain DMEA09 was isolated from traditional Korean fermented meju and its safety and technological properties were investigated. The DMEA09 strain showed good sensitivity to antibiotics, except for clindamycin. It did not exhibit hemolysis or biofilm formation, and did not possess virulence factors. Additionally, the DMEA09 strain demonstrated the ability to grow in the presence of salt and exhibited protease activity. Overall, DMEA09 is a safe and promising candidate for use as a starter in fermentation processes.
Article
Nutrition & Dietetics
Lingshuang Yang, Xinqiang Xie, Ying Li, Lei Wu, Congcong Fan, Tingting Liang, Yu Xi, Shuanghong Yang, Haixin Li, Jumei Zhang, Yu Ding, Liang Xue, Moutong Chen, Juan Wang, Qingping Wu
Summary: The study found that Enterococcus faecium strain 132 and Lactobacillus paracasei strain 201 can effectively lower blood lipids, improve cholesterol metabolism, and reduce fat accumulation. Additionally, these two strains can alleviate symptoms of hypercholesterolemia by regulating gut microbiota and increasing levels of acetic acid and propionic acid in the intestines.
Article
Microbiology
Ge Huang, Yizheng Zhou, Hai Cheng, Tao Lv, Lisi Zheng, Chengbin Li, Yunbo Chen
Summary: Through genomic and transcriptomic analysis, we found differences in carbon metabolism and amino acids between endogenously infected and only-colonized Enterococcus faecium, suggesting metabolic diversity as a strategy for endogenous infection.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Faezeh Salek, Hamid Mirzaei, Jalil Khandaghi, Afshin Javadi, Yousef Nami
Summary: The potential probiotic Enterococcus faecalis KUMS-T48, isolated from an Iranian traditional dairy product, has been found to have anti-pathogenic, anti-inflammatory, and anti-proliferative properties against specific cancer cell lines. The strain showed strong effects against Bacillus subtilis and Listeria monocytogenes, moderate effect against Yersinia enterocolitica, and weak effects against Klebsiella pneumoniae and Escherichia coli. Additionally, the cell-free supernatant of E. faecalis KUMS-T48 inhibited the proliferation of cancer cells in a similar manner to Taxol, but without affecting normal cell lines. The supernatant was found to have a proteinaceous nature and induced apoptosis-based cytotoxic mechanism through the regulation of specific genes.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Gregory S. Canfield, Anushila Chatterjee, Juliel Espinosa, Mihnea R. Mangalea, Emma K. Sheriff, Micah Keidan, Sara W. McBride, Bruce D. McCollister, Howard C. Hang, Breck A. Duerkop
Summary: Enterococcus faecium, a commensal of the human intestine, has evolved into a hospital-adapted, multidrug-resistant pathogen. Bacteriophages, natural predators of bacteria, show potential as therapeutics against MDR E. faecium infections, despite the unknown molecular events governing their interactions. While phage resistance may emerge, it is shown that lytic phages could effectively synergize with antibiotics to slow or inhibit E. faecium growth.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Weiyi Shen, Rong Zhang, Jiachang Cai
Summary: This study investigates the molecular characteristics and transferability of plasmid-borne linezolid resistance genes optrA, cfr, poxtA2, and cfr(D) in linezolid-resistant Enterococcus faecalis DM86 from retail meat. The presence of known linezolid resistance genes was detected using PCR analysis. Conjugation experiments were conducted to evaluate the transferability of these resistance genes. The complete genome of DM86 was obtained, and four linezolid resistance genes were identified on three plasmids.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Genetics & Heredity
Maria -Anna Misiakou, Frederik Boetius Hertz, Kristian Schonning, Susanne Haussler, Karen Leth Nielsen
Summary: This study aims to investigate the causes and mechanisms for the increase in linezolid resistance in E. faecium at Copenhagen University Hospital - Rigshospitalet. By analyzing the whole-genome sequencing data for E. faecium isolates collected since 2014, clusters of linezolid-resistant strains related to hospital transmission and de novo linezolid resistance were identified. Patients with the latter isolates were significantly more exposed to linezolid treatment. Additionally, a subset of patients initially carrying vancomycin-resistant, linezolid-sensitive enterococcus developed vancomycin-resistant, linezolid-resistant enterococci after linezolid treatment.
MICROBIAL GENOMICS
(2023)
Article
Biochemistry & Molecular Biology
Yating Du, Shiqi Luo, Xin Zhou
Summary: This study presents the first complete genome sequence of Enterococcus faecium isolated from honey bee gut, and investigates the interactions between E. faecium and honey bees through transcriptome and miRNA analysis. It was found that colonization of E. faecium increased honey bee gut weight, with up-regulation of developmental genes and enrichment of down-regulated miRNAs in developmental pathways. The study adds insights on how E. faecium affects honey bee gut weight at both transcriptional and post-transcriptional levels, as well as the role of miRNAs in mediating host and bacteria interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Infectious Diseases
Ana R. Freitas, Ana P. Pereira, Carla Novais, Luisa Peixe
Summary: Enterococcus faecium is a significant opportunistic human pathogen that has evolved towards a powerful adaptation to healthcare environments. However, the relevance of its dispersal in non-human hosts is still unknown.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Virology
S. Rigvava, I Kusradze, I. Tchgkonia, N. Karumidze, T. Dvalidze, M. Goderdzishvili
Summary: Enterococcus spp. is a common commensal microorganism, but some strains can cause opportunistic infections in humans. The spread of multi-resistant bacterial strains has renewed interest in phage therapy as a potential treatment for Enterococcus faecium infections.
Article
Biochemistry & Molecular Biology
Li Wang, Lin Qiao, Aike Li, Lixian Chen, Beibei He, Gang Liu, Weiwei Wang, Jun Fang
Summary: A high-temperature-resistant strain RS047-wl of Enterococcus faecium (E. faecium) was obtained through continuous heat-adaptation test. Multi-omics analysis revealed the involvement of 98 differentially expressed genes and 115 differential metabolites in regulating heat resistance, including the changes in AgrB, AgrC, and AgrA gene expressions in QS system pathways, accumulation of highly soluble osmotic substances, and down-regulation of organic acids metabolism and purine metabolism. These findings provide target genes for genetic modification and clues for screening heat-resistant bacteria to improve the heat resistance ability of E. faecium for production.
Article
Infectious Diseases
Desire Otsaghe Ekore, Richard Onanga, Pierre Phillipe Mbehang Nguema, Chloe Lozano, Brice Serge Kumulungui
Summary: The study investigated the antibiotics used in livestock and their impact on resistance in Enterococcus faecium and Enterococcus hirae on farms in Gabon. Tetracycline was the most commonly used antibiotic family and the Enterococcus isolates showed high resistance to tetracycline and rifampicin, with the tet(M) gene widely present in tetracycline-resistant isolates, especially in chickens. Antibiotic consumption in animals in Gabon was found to contribute to the emergence of antibiotic resistance.
Article
Microbiology
Li Deng, Wendong Zhen, Jing Wang, Dachuan Lin
Summary: In this study, a strain of Enterococcus faecium SZ21B15 was isolated from a bile sample of a patient with choledocholith in Shenzhen, China in 2021. The strain carried the oxazolidinone resistance gene optrA and showed intermediate resistance to linezolid. Whole-genome sequencing revealed that E. faecium SZ21B15 belonged to ST533 within the clonal complex 17. The optrA gene and two additional resistance genes fexA and erm(A) were located within a 25,777-bp multiresistance region, which was inserted into the chromosomal radC gene.
MICROBIOLOGY SPECTRUM
(2023)
Article
Food Science & Technology
Mina Nasiri, Shahram Hanifian
Summary: The study assessed the prevalence of enterococci in pasteurized milk samples from retail stores in Northwest Iran, finding that most isolates were identified as E. faecalis or E. faecium, carrying various virulence genes and showing high resistance to most antibiotics tested. Additionally, the isolates were able to produce biofilm, suggesting that pasteurized milk could potentially transmit multidrug-resistant virulent enterococci strains to humans.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Susana K. Checa, German F. Giri, Martin Espariz, Jose M. Arguello, Fernando C. Soncini
Summary: Copper (Cu) plays a significant role in the infection process of Salmonella, and through the co-regulation of specific factors, Salmonella is able to counteract the toxicity of copper.
TRENDS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
M. Saladrigas-Garcia, M. D'Angelo, H. L. Ko, S. Traserra, P. Nolis, Y. Ramayo-Caldas, J. M. Folch, P. Vergara, P. Llonch, J. F. Perez, S. M. Martin-Orue
Summary: The study aimed to investigate the impact of early socialization and an enriched neonatal environment on the adaptation of piglets to weaning. Post-weaning differences in microbial colonization, metabolic response, and gene expression were observed between piglets raised in barren vs enriched environments, indicating a possible link between environmental enrichment and improved adaptability to weaning stress.
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Dairy & Animal Science
Sandra Villagomez-Estrada, Jose F. Perez, Sandra van Kuijk, Diego Melo-Duran, Asal Forouzandeh, Francesc Gonzalez-Sole, Matilde D'Angelo, Francisco J. Perez-Cano, David Sola-Oriol
Summary: The study shows that partially replacing inorganic mineral sources with organic counterparts can improve neonate piglet gene expression, particularly in the smallest piglets of the litter, under commercial conditions.
JOURNAL OF ANIMAL SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Lucrecia C. Teran, Pablo Mortera, Gisela Tubio, Sergio H. Alarcon, Victor S. Blancato, Martin Espariz, Luis Esteban, Christian Magni
Summary: This study aimed to identify and evaluate lactic acid bacteria and yeast involved in the production of fermented feed, isolating Lactobacillus paracasei and Kazachstania unispora. Genome sequence analyses were performed to predict potential pathways and safety evaluations were conducted. The study concluded that these strains are good candidates for starter cultures in feed fermentation, providing valuable data for further biotechnological exploration.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Daniel Montoya, Matilde D'Angelo, Susana M. Martin-Orue, Agustina Rodriguez-Sorrento, Mireia Saladrigas-Garcia, Coralie Araujo, Thibaut Chabrillat, Sylvain Kerros, Lorena Castillejos
Summary: Phytogenic feed additives, derived from botanical sources, are increasingly used in pig diets, especially during the weaning period, to improve piglet adaptation and reduce the incidence of post-weaning diarrhea. Two plant-based supplements showed efficacy in enhancing piglet response to an ETEC F4 challenge through different mechanisms, with one improving microbiota balance and the other having anti-inflammatory properties.
Article
Multidisciplinary Sciences
M. Saladrigas-Garcia, M. D'Angelo, H. L. Ko, P. Nolis, Y. Ramayo-Caldas, J. M. Folch, P. Llonch, D. Sola-Oriol, J. F. Perez, S. M. Martin-Orue
Summary: This study investigated the impact of commercial early weaning on the gut microbiota, gene expression, and serum metabolomic response in piglets using an integrated-omic approach. The findings revealed significant changes in the gut microbiota and gene expression of the piglets after weaning, as well as notable alterations in metabolites. The results highlighted the complex interplay between gut microbiota, gene activity, and metabolic response in response to weaning stress.
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Dairy & Animal Science
Mireia Saladrigas-Garcia, David Sola-Oriol, Sergi Lopez-Verge, Matilde D'Angelo, Maria Carmen Collado, Bea Nielsen, Martin Faldyna, Jose Francisco Perez, Susana M. Martin-Orue
Summary: The aim of this study was to determine the effects of probiotic supplementation during gestation and lactation on the performance of sows and piglets, as well as the intestinal health of piglets after separation from the mother. Results showed that probiotic supplementation improved the reproductive performance of sows and had an impact on the fecal microbiota composition of both mothers and piglets.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Immunology
Maria Florencia Pacini, Florencia Belen Gonzalez, Brenda Dinatale, Camila Bulfoni Balbi, Silvina Raquel Villar, Cecilia Farre, Giuliana Lupi, Martin Espariz, Victor Sebastian Blancato, Christian Magni, Ivan Marcipar, Ana Rosa Perez
Summary: Intranasally-administered Trans-sialidase vaccines show promising immunogenicity and prophylactic efficacy against Trypanosoma cruzi, with TS + c-di-AMP formulation demonstrating the best protective capacity.
Article
Agriculture, Dairy & Animal Science
Asal Forouzandeh, Laia Blavi, Jose Francisco Perez, Matilde D'Angelo, Francesc Gonzalez-Sole, Alessandra Monteiro, Hans H. Stein, David Sola-Oriol
Summary: The study investigates the effects of adding copper sulfate or monovalent copper oxide to the diet of growing pigs on oxidative stress, inflammation, gene abundance, and microbial modulation. The results show that these additions can regulate gene abundance related to the immune system and growth, and promote changes in the intestinal microbiota. However, monovalent copper oxide induces less systemic oxidation and inflammation compared to copper sulfate.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Mariano Torres A. Manno, Fernan O. Gizzi, Mariana Martin, Martin Espariz, Christian Magni, Victor S. Blancato
Summary: In this study, the ruminal ecosystem of cows was thoroughly characterized using WGS metagenomic data to infer positive and negative livestock traits determined by the microbiome. Taxonomic characterization revealed the major phyla present, including Bacteroidetes and Firmicutes. Functional profiling of carbohydrate-active enzymes showed the efficient hydrolysis of complex biomass. The combination of bioinformatic analyses used in this study can guide enhanced nutrition and breeding methods.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Microbiology
Mireia Saladrigas-Garcia, Mario Duran, Matilde D'Angelo, Jaume Coma, Jose Francisco Perez, Susana Maria Martin-Orue
Summary: This study investigated the development of piglet gut microbiota from birth to weaning in commercial conditions, highlighting the impact of age on microbial diversity and the influence of farm environment on microbiota composition. Results showed a succession of microbial groups towards a more homogeneous ecosystem with increasing age, and differences in microbial colonization patterns and specific taxa between farms.