期刊
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷 191, 期 2, 页码 631-636出版社
SPRINGER
DOI: 10.1007/s12010-019-03211-x
关键词
Acetobacter; Bacterial cellulose; pH indicator; Screening; Methyl red
资金
- Science and Engineering Research Board [EMR/2016/002786] Funding Source: Medline
It is imperative that promising bacterial cellulose-producing bacteria mainly belongs to genera Acetobacter (acid-producing bacteria). In order to screen cellulose-producing Acetobacter, the isolated cultures from vinegar/rotten fruits were inoculated in Hestrin-Schramm (HS) medium containing ethanol and CaCO3. After the desired incubation, the positive cultures form a zone, which is observed around the bacterial growth, resulted from the solubilization of CaCO3 by acetic acid produced from the oxidation of ethanol during fermentation. However, in this method, the clarity of the solubilized zone is not very sharp and distinct. In the present, investigation, an improved method for screening, of the microorganisms producing acetic acid has been developed. In this method, methyl red (MR) is incorporated as a pH indicator in HS medium containing ethanol and CaCO3. Plates containing MR at alkaline pH are yellow and turn dark red at acidic pH. Thus, a distinctive, clear zone is formed around bacterial colonies producing acetic acid and is easy to differentiate between acid producers and non-producers. The present method is more rapid, accurate, and sensitive and can be successfully be used for the detection of acetic acid-producing bacteria particularly for the screening of potent cellulose producer Acetobacter sp.
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