Article
Microbiology
Kristyna Dufkova, Matej Bezdicek, Kristina Cuprova, Dagmar Pantuckova, Marketa Nykrynova, Eva Brhelova, Iva Kocmanova, Silvie Hodova, Marketa Hanslianova, Tomas Juren, Bretislav Lipovy, Jiri Mayer, Martina Lengerova
Summary: In this study, a novel molecular typing approach for Staphylococcus aureus was established, combining high-resolution melting analysis and multilocus sequence typing. This approach is rapid, reproducible, and cost-efficient, and has great potential for improving epidemiological investigations of S. aureus.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Han Zhang, Liyun Qin, Caiping Jin, Huidong Ju, Ruiping Jiang, Lijie Li, Hong Zhang, Weili Gao, Xiuping Wei, Hongyan Dong, Fei Lu, Guoping Lv
Summary: This study investigated the sequence typing and resistance profiles of Staphylococcus aureus isolates from patient and food samples in Shijiazhuang, China. The results showed a correlation between the isolates from patients and food samples, and Staphylococcus aureus exhibited resistance to multiple antibiotics.
Article
Biotechnology & Applied Microbiology
Heng Li, Taya Tang, Marc Stegger, Anders Dalsgaard, Tianlong Liu, Jorgen J. Leisner
Summary: The study found the presence of antimicrobial-resistant Staphylococcus aureus in sushi and pork products in Beijing, China, with some isolates originating from human sources. The transmission potential of antimicrobial-resistant S. aureus through foods was highlighted, emphasizing the importance of public health concerns. Geographical variation in ST profiles, antimicrobial resistance, and virulence genes of S. aureus between isolates from Beijing and Copenhagen, Denmark, was also demonstrated.
Article
Microbiology
Menghuan Yu, Haimei Shi, Hao Shen, Xueqin Chen, Li Zhang, Jianhua Zhu, Guoqing Qian, Bin Feng, Shaoning Yu
Summary: Oxacillin sodium salt combined with Gram staining and machine vision (MV) analysis is a new, simple and rapid method for identification of MRSA, which significantly reduces the time to detect resistance.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Tyler B. Clay, Kara W. Orwig, Raice A. Stevens, Emily P. Davis, Timothy M. Jennings, Timothy E. Long, Brittany L. Riley, Michael D. Hambuchen
Summary: MRSA PCR wound swabs showed high sensitivity, specificity, and predictive values in SSTIs, demonstrating their potential as a valuable tool.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2021)
Article
Pediatrics
Cortney Foster, Dayanand Bagdure, Jason Custer, Adrian Holloway, Peter Rycus, Jenni Day, Adnan Bhutta
Summary: In this study of 308 patients, there were no significant differences in outcomes for children receiving ECMO support with Staphylococcus aureus sepsis according to microbial methicillin sensitivity, with no increase in survival among patients with MRSA and MSSA infections.
FRONTIERS IN PEDIATRICS
(2021)
Article
Infectious Diseases
Beatriz Muller Nunes Souza, Juliana Guerra Pinto, Andre Henrique Correia Pereira, Alejandro Guillermo Minan, Juliana Ferreira-Strixino
Summary: The study found that the use of PDZ for antimicrobial photodynamic therapy has a significant bactericidal effect on Staphylococcus aureus, including complete inactivation of MRSA strains.
Article
Veterinary Sciences
Ting Gan, Gang Shu, Hualin Fu, Qigui Yan, Wei Zhang, Huaqiao Tang, Lizi Yin, Ling Zhao, Juchun Lin
Summary: S. aureus isolates from food animals in Sichuan province of China demonstrate severe antimicrobial resistance with various resistance genes, especially MRSA isolates. The genetic pool of MRSA isolates is diverse and complex, indicating the need for further investigation.
BMC VETERINARY RESEARCH
(2021)
Article
Microbiology
Aya Abd El Rahman, Yasmine El Kholy, Rania Y. Shash
Summary: The study revealed that methicillin-resistant Staphylococcus aureus (MRSA) is more proficient in biofilm-formation compared to methicillin-sensitive Staphylococcus aureus (MSSA). Furthermore, the expression of mazEF was significantly correlated to methicillin resistance in Staphylococcus aureus.
Article
Biochemistry & Molecular Biology
Fabien Lamret, Jennifer Varin-Simon, Melodie Six, Lea Thoraval, Julie Chevrier, Cloe Adam, Christine Guillaume, Frederic Velard, Sophie C. Gangloff, Fany Reffuveille
Summary: Osteoblasts and Staphylococcus aureus interactions can lead to impaired bone homeostasis. This study found that human osteoblast culture supernatant has a moderate effect on S. aureus biofilm formation, but does not significantly change the biofilm matrix or regulation of biofilm-related genes. Additionally, the sensitivity of S. aureus to bone cell secretions is strain-dependent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Morgan R. Bridwell, Sonal Bajaj, Todd W. Gress, Michael D. Hambuchen, Tyler B. Clay
Summary: MRSA PCR wound swab assays can rapidly detect MRSA and play a significant role in antibiotic usage reduction in skin infections.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2022)
Article
Infectious Diseases
Arianna Pani, Valeria Lucini, Silvana Dugnani, Francesco Scaglione
Summary: This study evaluated the effects of erdosteine in combination with different antibiotics against Staphylococcus aureus biofilms. The results showed that erdosteine enhanced the activity of antibiotics and reduced biofilm mass and bacterial viability. This suggests that erdosteine has potential clinical value in treating biofilm-related infections.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Review
Infectious Diseases
Daniele Roberto Giacobbe, Silvia Dettori, Silvia Corcione, Antonio Vena, Chiara Sepulcri, Alberto Enrico Maraolo, Francesco Giuseppe De Rosa, Matteo Bassetti
Summary: Staphylococcus aureus is an important human pathogen with a high mortality rate, and distinguishing between methicillin-susceptible and methicillin-resistant strains is crucial for treatment choices. The availability of approved agents with anti-MRSA activity allows for personalized treatment based on patient characteristics and evidence from controlled trials. In the treatment of S. aureus bloodstream infections, the development of new agents and the need for evidence on combination therapy are important considerations.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Clinical Neurology
Rodrigo Saad Berreta, Helen Zhang, Daniel Alsoof, Christopher L. McDonald, Bassel G. Diebo, Eren Kuris, Alan H. Daniels
Summary: This study investigated the epidemiological and microbiological landscape of spinal osteomyelitis and spondylodiscitis (SD), as well as the medical and surgical challenges in treating these infections. It found that more than 50% of SD cases were associated with Staphylococcus aureus, and there was an increasing prevalence of methicillin-resistant S. aureus (MRSA) in these infections. Substance abuse, such as alcohol, tobacco, and drug use, was a strong predictor for surgical intervention in SD cases.
JOURNAL OF NEUROSURGERY-SPINE
(2023)
Article
Immunology
Mehmet Demirci, Akin Yigin, Cemil Demir
Summary: This study investigated the antimicrobial and biofilm efficacy of the antimicrobial peptide LL-37 against biofilm-positive methicillin-susceptible S. aureus (MSSA) strains and biofilm-positive methicillin-resistant S. aureus (MRSA) strains, as well as its effects on gene expression levels. The results showed that LL-37 had inhibitory effects on biofilm formation in both MRSA and MSSA strains, and significantly affected gene expression levels in MRSA strains. The required antimicrobial concentration of LL-37 was high, but the antibiofilm concentration may be acceptable for use in humans.
MICROBIAL PATHOGENESIS
(2022)