Article
Chemistry, Multidisciplinary
Yuqiu He, Qing Wang, Chen Hong, Ruomeng Li, Jinhua Shang, Shanshan Yu, Xiaoqing Liu, Fuan Wang
Summary: Synthetic catalytic DNA circuits have potential for sensitive intracellular imaging, but their selectivity and efficiency are limited by off-site signal leakage and inefficient on-site activation. An endogenously activated DNAzyme strategy was integrated with a catalytic DNA circuit for selective and efficient microRNA imaging in vivo. The on-site modulation strategy allows these circuits to be expanded in biological systems.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Yuan Zhang, Chunmeng Ma, Chen Ma, Yueci Xiang, Shuai Mu, Zhixiang Zheng, Xiaoyan Liu, Haixia Zhang
Summary: The study utilized manganese dioxide nanosheets as carriers to deliver DNAzyme probes into cells, where intracellular glutathione reduced the nanosheets to release probes for miRNA detection. The newly proposed method offers a rapid and sensitive approach for imaging miRNA-155 in cancer cells, contributing to cancer diagnosis and biological research.
Article
Chemistry, Multidisciplinary
Qingqing Zhang, Shanshan Yu, Jinhua Shang, Shizhen He, Xiaoqing Liu, Fuan Wang
Summary: To improve the sensing performance of DNAzymes in live cells, a compact and robust Zn@DDz nanoplatform was developed. This platform enables amplified intracellular imaging of microRNA by controlling the disassembly of Zn@DDz nanoparticles. This work provides a promising approach for DNAzyme-based biosensing in living cells.
Review
Chemistry, Multidisciplinary
Mengyuan Li, Lele Li
Summary: With the programmability of DNA, sensors can be developed for specific disease cells and spatially controlled molecular imaging. By combining DNA with enzymes, the limitations of DNA-based sensors can be overcome, allowing for improved cell specificity and signal-to-background ratio.
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Chemistry, Analytical
Yu-Wen Zhang, Shan Li, Shu-Min Wang, Xiao-Qiong Li, Mei-Rong Cui, Bin Kang, Hong-Yuan Chen, Jing-Juan Xu
Summary: We developed a miRNA-responsive DNAzyme cascaded catalytic (MDCC) nanomachine for amplified intracellular miRNA imaging and miRNA-guided efficient gene silencing. The intelligent MDCC nanomachine consists of multiple DNAzyme subunit-encoded catalyzed hairpin assembly (CHA) reactants sustained by pH-responsive Zeolitic imidazolate framework-8 (ZIF-8) nanoparticles. Through a catalytic hairpin assembly (CHA) reaction triggered by the presence of miRNA-21, a large number of Y-shaped fluorescent DNA constructs containing three DNAzyme modules are generated for gene silencing. This strategy provides a promising platform for highly sensitive determination of biomolecules and accurate gene therapy of cancer cells.
Article
Chemistry, Multidisciplinary
Yanlei Hu, Xia Chu
Summary: DNA walkers, imitating protein motors, are a type of nucleic acid nanodevice that can move on cell membranes and be used for cell membrane target sensing, showing great potential for applications in materials and biotechnology.
Article
Chemistry, Analytical
Ting Zhou, Mengqi Huang, Jinqiong Lin, Ru Huang, Da Xing
Summary: Exploring the trans-cleavage ability of Cas13a and developing a specific and sensitive visual detection system for miRNA, called vCas, with a detection limit of 1 fM and single-base specificity, suggests a promising application in clinical molecular diagnosis.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Qing Wang, Zeyue Wang, Yuqiu He, Bin Xiong, Yingfu Li, Fuan Wang
Summary: DNAzyme is a single-stranded catalytic DNA with various physical and chemical features. Efforts have been made to incorporate RNA-cleaving DNAzyme (RCD) into biosensors and biomedical research, but its in vivo application is limited by off-target activation. Therefore, there is a need to develop spatiotemporally controlled DNAzyme. This paper provides an overview of various methods for specific stimulation/regulation of RCD activities and reviews examples of stimuli-responsive biosensing and biomedical research using chemically and/or structurally caged RCD within the past five years.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Huimin Wang, Yuqiu He, Jie Wei, Hong Wang, Kang Ma, Yangjie Zhou, Xiaoqing Liu, Xiang Zhou, Fuan Wang
Summary: This study developed a versatile autocatalytic DNA circuit for high-performance in vivo bioanalysis. The circuit showed sensitive and selective detection of analytes, and enabled robust miRNA imaging in living cells and mice. These findings expand the toolbox for accurate diagnosis and programmable therapeutics.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Jie Wei, Mengdi Yu, Kaiyue Tan, Jinhua Shang, Shizhen He, Chenxia Xie, Xiaoqing Liu, Fuan Wang
Summary: Trace analyte detection in complex intracellular environment requires the development of simple yet robust self-sufficient molecular circuits with high signal-gain and anti-interference features. A minimal non-enzymatic self-replicate DNA circuitry (SDC) system with high-signal-gain for highly efficient biosensing in living cells is proposed. The SDC amplifier enables the high-contrast and accurate visualization of microRNA (miRNA), offering a promising strategy for monitoring clinically significant analytes.
Article
Chemistry, Analytical
Yunfeng Bai, Huilin Zhang, Lu Zhao, Yuzhen Wang, Xiaoliang Chen, Hong Zhai, Maozhong Tian, Ruirui Zhao, Tao Wang, Hui Xu, Feng Feng
Summary: This study developed a rationally designed aptasensing platform based on HCR and G-quadruplex DNAzyme for the fluorescence detection of CEA. The platform showed high selectivity for CEA and was successfully applied in diluted human serum samples, demonstrating promising potential in biochemical analysis and clinic application.
Article
Chemistry, Multidisciplinary
Jiayao Xu, Yuxin Qin, Qiuyi Liang, Xiaohong Zhong, Li Hou, Yong Huang, Shulin Zhao, Hong Liang
Summary: A novel MnOx-nucleic acid nanoprobe was developed for catalytic imaging of microRNA in living cells by combining catalytic hairpin assembly, hybridization chain reaction, and DNAzyme amplification. The nanoprobe exhibited ultrahigh sensitivity and specificity, and could differentiate tumor cells from normal cells through live cell microRNA imaging.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Applied
Yahui Guo, Panting Sang, Gang Lu, Xue Yang, Yunfei Xie, Zhigang Hu, He Qian, Weirong Yao
Summary: An artificial deoxyribozyme was used as a substitute for horseradish peroxidase (or alkaline phosphatase) in ELISA for generating amplified signals. The feasibility of the proposed deoxyribozyme-based ELISA (DLISA) was demonstrated in the detection of a forbidden veterinary drug, chloramphenicol. With a detection limit of 0.1 ng/L and a wide linear range from 0.000001 μg/mL to 1.0 μg/mL, DLISA showed excellent practical performances. The easy connection of antibody and DNAzyme through gold nanoparticles in DLISA made it worth popularizing.
Article
Chemistry, Multidisciplinary
Yu Shang, Yingying Chen, Qing Wang, Yuqiu He, Shizhen He, Shanshan Yu, Xiaoqing Liu, Fuan Wang
Summary: This study developed an endogenously and sequentially activated DNA circuit for in vivo imaging of tumor cells with high precision and reliability. The smart DNA circuit, engineered using a specific enzymatic regulation strategy, ensured the specific reaction and sensitive imaging, providing a powerful tool for cancer diagnosis and treatment.
Article
Spectroscopy
Jiaqi Xu, Xi Zhou, Hailun He, Shanni Li, Changbei Ma
Summary: In this study, a novel fluorescence strategy for hypochlorous acid (HClO) detection was developed based on nucleic acid oxidative cleavage and Pb2+-dependent DNAzyme. Monitoring HClO concentration is highly significant in healthcare and pathophysiology research. The method demonstrated exceptional performance and showed great potential for use in clinical diagnosis and medical research.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Nanoscience & Nanotechnology
Tianhui Shi, Jialing Hu, Wenxiao Wang, Qunying Jiang, Zhen Xu, Shuyi Yu, Fuan Wang, Xiaoqing Liu
Summary: A Met-targeted nanoinhibitor was developed to efficiently suppress metastasis by multiple blockading HGF/Met signaling pathway. This method showed remarkable inhibition of tumor metastasis in an orthotopic breast tumor model.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Nanoscience & Nanotechnology
Yuhui Gao, Yingying Chen, Jinhua Shang, Shanshan Yu, Shizhen He, Ran Cui, Fuan Wang
Summary: Aptsensors with high specificity have great potential for clinical diagnosis and prognosis. However, their applications in intracellular molecular imaging are limited. In this study, a robust enzyme-free autocatalysis-driven feedback DNA circuit was developed for amplified aptasensing with significantly improved sensitivity in living cells.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Wenqian Yu, Junlin Sun, Xiuyuan Wang, Shuyi Yu, Mingzhu Yan, Fuan Wang, Xiaoqing Liu
Summary: This study reports a convenient tumor-specific A2AR inhibition strategy to improve antitumor immune responses using a photo-modulated nanoreactor for spatiotemporally controlled oxygen supply. This nanoreactor not only relieves A2AR-mediated immunosuppression, but also enhances antitumor immunity through photothermal ablation of the tumor and immunogenic cell deaths.
ADVANCED MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Huimin Wang, Yuqiu He, Jie Wei, Hong Wang, Kang Ma, Yangjie Zhou, Xiaoqing Liu, Xiang Zhou, Fuan Wang
Summary: This study developed a versatile autocatalytic DNA circuit for high-performance in vivo bioanalysis. The circuit showed sensitive and selective detection of analytes, and enabled robust miRNA imaging in living cells and mice. These findings expand the toolbox for accurate diagnosis and programmable therapeutics.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Yun Zhao, Ruomeng Li, Junlin Sun, Zhiqiao Zou, Fuan Wang, Xiaoqing Liu
Summary: This study developed a versatile theranostic agent to combat metastatic tumors. By delivering a specific DNAzyme, it effectively inhibits the invasion of cancer cells through gene silencing. The combined gene/chemodynamic therapy is achieved by self-supplied DNAzyme cofactors and efficient glutathione depletion. Additionally, the photothermal stimuli further promote the therapeutic strategy and accelerate the release of DNAzyme, resulting in effective tumor elimination and metastasis blockage.
Article
Chemistry, Analytical
Fengzhe Li, Yingying Chen, Jinhua Shang, Qing Wang, Shizhen He, Xiwen Xing, Fuan Wang
Summary: This paper describes an AHR circuit for the sensitive detection of MTase, which combines the CHA converter with the HCR amplifier. By introducing an auxiliary hairpin, the assay of MTase can be mediated through the AHR circuit. This method has the ability to sensitively, robustly, and versatilely analyze MTase, holding great potential for biomedical analysis and clinical diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Huiran Yang, Benrui Weng, Sijia Liu, Nana Kang, Jiabing Ran, Zhangshuang Deng, Huimin Wang, Changying Yang, Fuan Wang
Summary: In this study, we constructed an acid-improved DNAzyme-based isothermal enzyme-free concatenated DNA circuit for miRNA detection. The method achieved significantly reduced background and improved signal-to-noise ratio through catalyzed hairpin assembly (CHA) and hybridization chain reaction (HCR).
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Qiong Wu, Lei Yang, Lingling Xie, Jinhua Shang, Shizhen He, Jing Liu, Fuan Wang
Summary: This research presents a multilayer nonenzymatic catalytic DNA circuits-based aptasensing system, which enables in situ imaging of bioactive molecules in living mice. The aptasensing system shows high sensitivity, accuracy, and adaptability, making it suitable for analyzing various bioactive molecules.
Article
Nanoscience & Nanotechnology
Yushi Wang, Yingying Chen, Yeqing Wan, Chen Hong, Jinhua Shang, Fengzhe Li, Xiaoqing Liu, Fuan Wang
Summary: This study proposes an autocatalytic hybridization system that combines hybridization chain assembly and catalytic DNA assembly for highly efficient positive feedback amplification. The system allows sensitive detection of polynucleotide kinase and has the potential for accurate monitoring and screening in vitro and in biological samples.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Shanshan Yu, Jinhua Shang, Shizhen He, Qing Wang, Ruomeng Li, Yingying Chen, Xiaoqing Liu, Fuan Wang
Summary: In this study, a high-contrast in vivo imaging of microRNA (miRNA) was achieved by integrating an entropy-driven catalytic DNA amplification machine with on-site amplified substrate exposure procedure. The undesired signal leakage was eliminated by grafting substrate subunits into an auxiliary DNA polymerization amplification accessory. The intact machine-propelling functions were restored by cell-specific mRNA, ensuring the high specificity of on-site amplified miRNA imaging. This intelligent machinery shows great potential in biomedical diagnosis.
Article
Engineering, Environmental
Jialing Hu, Zhiqiao Zou, Fengye Mo, Xue Lin, Yun Zhao, Tianhui Shi, Fuan Wang, Xiaoqing Liu
Summary: A DNA prodrug-laden metallo-nanoassembly has been developed for sensitive activation of DNAzymes to regulate disease genes, with enhanced gene cleavage activity and improved drug stability.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Chemistry, Multidisciplinary
Wei Xu, Feifei Hu, Jiajing Li, Jinhua Shang, Xiaoqing Liu, Yan Zeng, Qiong Wu, Fuan Wang
Summary: This article provides an overview of recent advances in stimuli-responsive DNA devices that can respond to a variety of external triggers. These triggers include physical stimuli, chemical stimuli, and biological stimuli, and the DNA devices have controllable nanofabrication and biomedical applications.
SCIENCE CHINA-CHEMISTRY
(2023)
Review
Chemistry, Multidisciplinary
Siyuan Wang, Jinhua Shang, Bingyue Zhao, Huimin Wang, Changying Yang, Xiaoqing Liu, Fuan Wang
Summary: This Review summarizes the important role of isothermal enzyme-free nucleic acid amplification in biosensing, describes the mechanisms of entropy-driven reaction, hybridization chain reaction, catalytic hairpin assembly, and DNAzyme, and comprehensively discusses their recent applications as biosensors. It also discusses the current problems and developments in these DNA circuits.
Review
Chemistry, Analytical
Siyuan Wang, Yaqi Liu, Jinhua Shang, Huimin Wang, Changying Yang, Xiaoqing Liu, Fuan Wang
Summary: This article provides a comprehensive review of DNAzymes, including their in vitro selection methods and activity regulation strategies. The recent advances in DNAzyme-based biosensors and therapeutic applications are summarized, along with discussion on current challenges and future development.
ANALYSIS & SENSING
(2023)
Article
Chemistry, Multidisciplinary
Dan Luo, Xue Lin, Yun Zhao, Jialing Hu, Fengye Mo, Gege Song, Zhiqiao Zou, Fuan Wang, Xiaoqing Liu
Summary: DNAzyme-mediated gene regulation and photodynamic cancer therapy achieved using dynamic DNA nanomaterials with self-enhanced efficacy, providing a potential therapeutic approach for gene modulation.
