期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 59, 期 10, 页码 3956-3960出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201915555
关键词
CRISPR-Cas; DNA nanotechnology; DNA origami; molecular devices; self-assembly
资金
- National Institutes of Health (NIH) Director's New Innovator Award [GM114830]
- NIH [GM132114]
- Yale University
- Agency for Science, Technology and Research Graduate Scholarship (Singapore)
- China Scholarship Council fellowship
- National Science Foundation Graduate Fellowship
Customizable nanostructures built through the DNA-origami technique hold tremendous promise in nanomaterial fabrication and biotechnology. Despite the cutting-edge tools for DNA-origami design and preparation, it remains challenging to separate structural components of an architecture built from-thus held together by-a continuous scaffold strand, which in turn limits the modularity and function of the DNA-origami devices. To address this challenge, here we present an enzymatic method to clean up and reconfigure DNA-origami structures. We target single-stranded (ss) regions of DNA-origami structures and remove them with CRISPR-Cas12a, a hyper-active ssDNA endonuclease without sequence specificity. We demonstrate the utility of this facile, selective post-processing method on DNA structures with various geometrical and mechanical properties, realizing intricate structures and structural transformations that were previously difficult to engineer. Given the biocompatibility of Cas12a-like enzymes, this versatile tool may be programmed in the future to operate functional nanodevices in cells.
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