期刊
ANALYTICAL CHEMISTRY
卷 92, 期 5, 页码 4101-4107出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b05771
关键词
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资金
- National Natural Science Foundation of China [21675105, 21535004, 91753111, 21927811]
- Key Research and Development Program of Shandong Province [2018YFJH0502]
- National Major Scientific and Technological Special Project for Significant New Drugs Development [2017ZX09301030004]
- Natural Science Foundation of Shandong Province of China [ZR2017ZC0225]
Depression is immensely attributed to the over-activation of N-methyl-D-aspartic acid (NMDA) receptor in the brains. As regulatory binding partners of NMDA receptor, both Zn2+ and H+ are intimately interrelated to NMDA receptor's activity. Therefore, exploring synergistic changes on the levels of Zn2+ and H+ in brains will promote the knowledge and treatment of depression. However, the lack of efficient, appropriate imaging tools limits simultaneously tracking Zn2+ and H+ in living mouse brains. Thus, a well-designed dual-color fluorescent probe (DNP) was fabricated for the simultaneous monitoring of Zn2+ and H+ in the brains of mice with depression. Encountering Zn2+, the probe evoked bright blue fluorescence at 460 nm. Meanwhile, the red fluorescence at 680 nm was decreased with H+ addition. With blue/red dual fluorescence signal of DNP, we observed the synchronous increased Zn2+ and H+ in PC12 cells under oxidative stress. Notably, in vivo imaging for the first time revealed the simultaneous reduction of Zn2+ and pH in brains of mice with depression-like behaviors. Further results implied that the NMDA receptor might be responsible for the coinstantaneous fluctuation of Zn2+ and H+ during depression. Altogether, this work is conducive to the knowledge of neural signal transduction mechanisms, advancing our understanding of the pathogenesis in depression.
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