期刊
ANALYTICAL CHEMISTRY
卷 92, 期 1, 页码 1611-1617出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b04927
关键词
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资金
- National Natural Science Foundation of China [81973050, 31871881]
- National Key Research and Development program of China [2017YFC1200903]
- Key Research and Development Program of Tianjin [417 18YFZCNC01260]
- Jiangsu Agriculture Science and Technology Innovation Fund (JASTIF) [CX(18)2025]
- S&T Support Program of Jiangsu Province [BE2017623]
- National First-class Discipline Program of Food Science and Technology [JUFSTR20180303]
- Distinguished Professor Program of Jiangsu Province
Self-assembled polydiacetylene (PDA) vesicles, with the distinct advantages of low-cost materials, simple preparation, and excellent chromatic properties, can be perfectly combined with a colorimetric strip for on-site inspection. Herein, without involving expensive reagents and instruments, a visual colorimetric strip based on well-prepared PDA vesicles was developed to analyze and monitor histamine in deep-sea fish and its canned food. The standard calorimetric card for semiquantitative detection of histamine was successfully prepared and the quantitative detection can be further realized by analyzing the gray value using ImageJ and Color Grab in a smart phone. After optimizing the assembly conditions, this assay exhibited a linear response to histamine within the range from 70 to 2240 ppm. With excellent stability and sensitivity, this strip can be used to monitor the quality change of canned fish at different temperatures, so that people can avoid suffering from histamine poisoning, suggesting that it holds great potential in the intelligent system for on-site detection and real-time monitoring.
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