期刊
ANALYTICAL CHEMISTRY
卷 92, 期 4, 页码 2972-2978出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b03677
关键词
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资金
- National Natural Science Foundation of China [81672570, 81772593]
In this work, the reduced metal-organic frameworks (MOFs) that can function as a three-dimensional catalytic nanoreactor for copper-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction have been fabricated for development of the immunoassay. In our design, MOFs with well-defined porosity are accessible to the substrates of CuAAC, while the substrates can diffuse in the pore channel. Moreover, the high-ordered structural alignments of MOFs are beneficial for integration of copper ions into the three-dimensional lattice. Therefore, upon exposure to reduction, the copper nodes in MOFs are transformed from Cu-II to Cu-I in situ to achieve spatiotemporal control, eliminating the primarily dissolution procedure. Furthermore, the reduced MOFs can serve as a powerful catalyst to facilitate the CuAAC click reaction, which can greatly reduce time and improve the reaction efficiency. In addition, the proposed immunoassay shows high analytical performance for hepatitis B virus surface antigen (HBsAg) quantification in the linear range of 0.03-2.0 ng mL(-1) with a detection limit down to 11.2 pg mL(-1). The new immunoassay has also been challenged in detection of clinical serum sample, and a good consistency can be found with the time-resolved fluorescence immunoassay (TRFIA) in clinics, signifying its promising potential in clinical diagnosis.
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