Article
Biochemistry & Molecular Biology
Marcel Zamocky, Jana Harichova
Summary: We reconstructed the molecular phylogeny of heme containing peroxygenases, revealing high diversity and conserved sequence motifs. The genes coding for these peroxygenases were detected mainly in fungi, but also in other lower eukaryotes. The phylogenetic analysis can be useful for designing modified peroxygenases for future biotech applications.
Article
Biochemistry & Molecular Biology
Andrej Poljovka, Milos Musil, David Bednar, Katarina Chovanova, Vladena Bauerova-Hlinkova, Jana Bellova, Lenka Kohutova, Peter Barath, Marcel Zamocky
Summary: The study found that the catalase-peroxidases from thermophilic and mesophilic fungi have similar active center structures and peroxidase activity, while the thermophilic variant CthedisKatG showed enhanced manganese peroxidase activity at higher temperatures. Two constructed mutations completely abolished catalatic activity and altered the peroxidase activity, suggesting the potential biotech applications of bifunctional heme enzymes with variable reactivity.
Review
Chemistry, Multidisciplinary
Namrata Singh, G. R. Sherin, Govindasamy Mugesh
Summary: Nanozymes, nanomaterials with enzyme-mimicking activity, have attracted significant interest in biomedical applications, particularly in oxidative stress-mediated disorders. The challenge lies in designing nanozymes that can selectively differentiate between physiological oxidative stress and pathological oxidative stress and mediate appropriate redox reactions in cells, tissues, and organs. This review summarizes the advancements in redox-active nanozymes and their therapeutic significance in various disease models, such as cancer, neurodegeneration, and cardiovascular diseases. It also discusses the future prospects and challenges associated with the biomedical applications of nanozymes.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biology
Marcel Zamocky, Milos Musil, Maksym Danchenko, Peter Ferianc, Katarina Chovanova, Peter Barath, Andrej Poljovka, David Bednar
Summary: This study presents a detailed bioinformatics analysis of Hybrid B heme peroxidases in the fungal family Sclerotiniaceae. The research reveals the evolutionary pattern of these peroxidases in phytopathogens and identifies similar peroxidase sequences in different fungal species. The study also highlights minor yet important modifications in the active center architecture of these peroxidases.
Article
Horticulture
Mohammad Pourebrahimi, Saeid Eshghi, Asghar Ramezanian, Shiva Faghih
Summary: High salinity levels can lead to various adverse effects on plants, including osmotic stress and nutritional disorders. In this study, the combined use of hydrogen sulfide and selenium compounds was found to alleviate the negative effects of salt stress on strawberry yield and enhance the antioxidant and nutritional quality of the fruits.
SCIENTIA HORTICULTURAE
(2023)
Review
Biochemistry & Molecular Biology
Pavlina Hemerkova, Martin Valis
Summary: ALS is a neurodegenerative disease that affects motor neurons and currently has no cure. Free oxygen radicals are known to play a role in the pathogenesis of ALS, while antioxidant enzymes like SOD1 are crucial for antioxidant protection.
Review
Biochemistry & Molecular Biology
Yu Ah Hong, Cheol Whee Park
Summary: Reactive oxygen and nitrogen species play crucial roles in kidney injuries, making antioxidant agents, especially catalytic antioxidants that mimic antioxidant enzymes, effective in combating oxidative stress-related kidney diseases. Research has shown the effectiveness of several catalytic antioxidants in treating disease models associated with oxidative stress.
Review
Biochemistry & Molecular Biology
Diana Ofelia Lopez-Cantu, Reyna Berenice Gonzalez-Gonzalez, Elda M. Melchor-Martinez, Saul Antonio Hernandez Martinez, Rafael G. Araujo, Lizeth Parra-Arroyo, Juan Eduardo Sosa-Hernandez, Roberto Parra-Saldivar, Hafiz M. N. Iqbal
Summary: Nanozymes, artificial enzymes based on engineered nanomaterials, have been developed to overcome inherent drawbacks of natural enzymes, showing great potential for various applications. Carbon dots have garnered significant attention for their high catalytic activity and biocompatibility, serving as possible alternatives in biomedical and environmental fields. Studies have shown that carbon dots can mimic different oxidoreductases, exhibiting multiple enzyme-like activities.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Veterinary Sciences
Rejane Santos Sousa, Caroline Santos Sousa, Francisco Leonardo Costa Oliveira, Paulo Ricardo Firmino, Isadora Karolina Freitas Sousa, Valeria Veras Paula, Nohora Mercado Caruso, Enrico Lippi Ortolani, Antonio Humberto Hamad Minervino, Raimundo Alves Barreto-Junior
Summary: Blood loss in sheep can cause clinical, hematological, blood gas, and biochemical alterations, leading to oxidative stress and reduced antioxidant activity.
VETERINARY SCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Zi-Yang Liao, Wei-Wei Gao, Ning-Ning Shao, Jia-Min Zuo, Tao Wang, Meng-Zhen Xu, Feng-Xiu Zhang, Ya-Mu Xia
Summary: FePO4-HG, a new antibacterial agent, has demonstrated excellent antibacterial ability against antibiotic-resistant pathogens and can promote wound healing.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Biochemistry & Molecular Biology
Angelo Zinellu, Arduino A. Mangoni
Summary: The systematic review and meta-analysis showed that statins significantly increased the concentrations of glutathione peroxidase and superoxide dismutase in the blood, indicating an antioxidant effect. However, the impact on catalase was minimal.
Article
Biology
Aehsan ul Haq, Mohammad Lateef Lone, Sumira Farooq, Shazia Parveen, Foziya Altaf, Inayatullah Tahir, Prashant Kaushik, Hamed A. El-Serehy
Summary: Recent studies have found that salicylic acid (SA) can reduce postharvest losses in various flower systems. This study focused on the role of SA in enhancing physiological and biochemical responses to mitigate postharvest senescence in cut spikes of Consolida ajacis. The results showed that SA treatment increased the postharvest longevity of the cut spikes, as well as the solution uptake, sugar, and protein content of the tepal tissues. SA also enhanced the antioxidant system and reduced lipoxygenase activity, preserving membrane integrity and preventing solute leakage from the tepal tissues.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Nanoscience & Nanotechnology
Polina Ivanova, Konrad Dziegielewski, Marcin Drozd, Sandra Skorupska, Ilona Grabowska-Jadach, Mariusz Pietrzak
Summary: Reactive oxygen species (ROS) are important in physiological processes but can pose a threat to health with increased concentrations. Different defense systems in plants, invertebrates, and vertebrates including humans combat ROS, but environmental conditions and lifestyle can interfere with enzyme activity. Therefore, finding alternative ROS scavengers is crucial, with nanozymes being a proposed solution to mimic essential enzyme activities and prevent excessive ROS activity.
Article
Environmental Sciences
Yue-Yue Zhou, You-Shao Wang, Aniefiok Ini Inyang
Summary: The study found that protein concentrations were significantly higher in Bruguiera gymnorrhiza, Kandelia obovata, and Rhizophora stylosa under heavy metal stress conditions, while Avicennia marina had remarkably high MDA content. Superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) levels were significantly elevated in A. marina, A. corniculatum, and B. gymnorrhiza. Principal component analysis indicated that POD, SOD, and MDA were major indices for response to heavy metal stress, with A. marina demonstrating higher tolerance compared to other species.
MARINE POLLUTION BULLETIN
(2021)
Review
Chemistry, Analytical
Nguyen Thi My Thao, Hoang Dang Khoa Do, Nguyen Nhat Nam, Nguyen Khoi Song Tran, Thach Thi Dan, Kieu The Loan Trinh
Summary: Antioxidant nanozymes have emerged as promising materials to replace natural antioxidant enzymes due to their stability, cost savings, and flexible design. This review discusses the mechanisms and manipulation strategies of antioxidant nanozymes, as well as their potential applications in medicine and healthcare. Overall, this review provides useful information for the further development and application of antioxidant nanozymes.
Article
Biochemistry & Molecular Biology
Stefan Hofbauer, Marcello Pignataro, Marco Borsari, Carlo Augusto Bortolotti, Giulia Di Rocco, Gianina Ravenscroft, Paul G. Furtmuller, Christian Obinger, Marco Sola, Gianantonio Battistuzzi
Summary: The autosomal dominant striated muscle disease myoglobinopathy is caused by a single point mutation in human myoglobin, leading to increased reactivity towards hydrogen peroxide, enhanced tendency to form high-molecular-weight aggregates, and accelerated heme bleaching. These effects contribute to impaired oxygen binding capacity and faster heme dissociation, ultimately impacting the pathological symptoms of myoglobinopathy.
Editorial Material
Biochemistry & Molecular Biology
Christian Obinger
Summary: This paper presents the first purification and characterization of a manganese peroxidase, demonstrating its ability to oxidize various compounds using hydrogen peroxide and Mn(II). This discovery in 1985 laid the foundation for extensive research on the structure-function relationships, ecological roles, and industrial applications of manganese peroxidases.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Biology
Marcel Zamocky, Milos Musil, Maksym Danchenko, Peter Ferianc, Katarina Chovanova, Peter Barath, Andrej Poljovka, David Bednar
Summary: This study presents a detailed bioinformatics analysis of Hybrid B heme peroxidases in the fungal family Sclerotiniaceae. The research reveals the evolutionary pattern of these peroxidases in phytopathogens and identifies similar peroxidase sequences in different fungal species. The study also highlights minor yet important modifications in the active center architecture of these peroxidases.
Article
Biochemistry & Molecular Biology
Douglas Jardim-Messeder, Marcel Zamocky, Gilberto Sachetto-Martins, Marcia Margis-Pinheiro
Summary: This article analyzes and discusses the origin, evolution, and function of the APX family. The study found that APX originated as a soluble enzyme and evolved to have targeting sequences for chloroplasts and mitochondria. Independent gene duplications and neofunctionalization events in certain angiosperm groups resulted in the development of APX isoforms localized in different subcellular compartments. The complexity of APX isoforms contributes to the adaptation of diverse plant species to changing environmental conditions.
Review
Gastroenterology & Hepatology
Monika Kvakova, Anna Kamlarova, Jana Stofilova, Veronika Benetinova, Izabela Bertkova
Summary: Colorectal cancer is a major cause of death worldwide. Conventional anticancer therapy is not always effective, leading to growing interest in drug-free cancer treatments or interventions that improve the efficacy of established therapy. Supplementing patient treatment with probiotics, next-generation probiotics, or postbiotics can restore gut microbiota composition and/or signaling to the host, providing an effective and accessible complementary anticancer strategy.
WORLD JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anja Wagner, Edgar Galicia-Andres, Magdalena Teufl, Lukas Gold, Christian Obinger, Peter Sykacek, Chris Oostenbrink, Michael W. Traxlmayr
Summary: By screening a mutated EGFR library, previously unknown activating mutations in EGFR were discovered. These mutations are not only located in the kinase domain of EGFR, but also in other regions, including the extracellular and transmembrane domains.
Review
Biochemistry & Molecular Biology
Jana Stofilova, Monika Kvakova, Anna Kamlarova, Emilia Hijova, Izabela Bertkova, Zuzana Gul'asova
Summary: There is a need for new therapies for ulcerative colitis, as many patients are unresponsive to available treatments. Strategies targeting dysbiosis of gut microbiota have been tested, but the effectiveness of conventional probiotics varies. New candidates for next-generation probiotics could provide more targeted treatment options.
Article
Biochemistry & Molecular Biology
Daniel Schmidt, Nikolaus Falb, Ilenia Serra, Marzia Bellei, Vera Pfanzagl, Stefan Hofbauer, Sabine Van Doorslaer, Gianantonio Battistuzzi, Paul G. Furtmuller, Christian Obinger
Article
Biochemistry & Molecular Biology
Daniel R. Ramos, Paul G. Furtmueller, Christian Obinger, Angeles Pena-Gallego, Ignacio Perez-Juste, J. Arturo Santaballa
Summary: Electronic structure calculations using DFT were conducted to examine the influence of water molecules and protonation on the heme group of peroxidases in different redox and spin states. The study discusses shared geometries, spectroscopic properties, and thermodynamics of peroxidases. Computed Gibbs free energies suggest that the corresponding aquo complexes are not thermodynamically stable, supporting the five-coordinate Fe(III) center in native ferric peroxidases with a non-bonding water molecule. Protonation of the ferryl oxygen of compound II is found to be necessary and computed Gibbs free energies reveal pK(a) values of approximately 8.5-9.0 for compound II.
Article
Biochemistry & Molecular Biology
Douglas Jardim-Messeder, Andreia Caverzan, Natalia Balbinott, Paloma K. Menguer, Ana L. S. Paiva, Moaciria Lemos, Juliana R. Cunha, Marcos L. Gaeta, Miguel Costa, Marcel Zamocky, Nelson J. M. Saibo, Joaquim A. G. Silveira, Rogerio Margis, Marcia Margis-Pinheiro
Summary: This study found that silencing the OsAPX7 gene in rice does not affect plant growth, redox state, or photosynthesis parameters. However, silenced plants (APX7i) show higher tolerance to drought stress, with a higher ability to maintain stomata aperture and photosynthesis performance compared to non-transformed plants. RNA-seq analyses indicate that the silencing of OsAPX7 does not alter the global expression of genes related to reactive oxygen species metabolism. Additionally, the drought-induced regulation of genes related to proteasome pathway, nitrogen metabolism, and carotenoid metabolism is impaired in APX7i plants. On the other hand, genes associated with flavonoid and tyrosine metabolism are upregulated, while genes related to phytohormone signal transduction and nicotinate and nicotinamide metabolism are downregulated in APX7i during drought stress. These results suggest that OsAPX7 may be involved in signaling transduction pathways related to drought stress response in rice, providing insights into the physiological role of chloroplast APX isoforms.
Editorial Material
Biochemistry & Molecular Biology
Marcel Zamocky
Article
Biology
Douglas Jardim-Messeder, Ygor de Souza-Vieira, Lucas Correa Lavaquial, Daniela Cassol, Vanessa Galhego, Gabriel Afonso Bastos, Thais Felix-Cordeiro, Regis Lopes Correa, Marcel Zamocky, Marcia Margis-Pinheiro, Gilberto Sachetto-Martins
Summary: Climate changes threaten agriculture and plants need to cope with different environmental conditions, including reactive oxygen species (ROS). The ascorbate-glutathione cycle, composed of APX, MDAR, DHAR, and GR enzymes, is the main antioxidant pathway in plants. This study identified and classified these genes in multiple plant species, predicted their subcellular localization, and contributed to understanding their evolutionary history. The expression pattern of these genes in response to drought stress in castor bean was also analyzed. Overall, this study provides important insights into the regulation of ROS metabolism in drought tolerance.
Article
Biochemistry & Molecular Biology
Zuzana Farkas, Andrea Puskarova, Alena Opalkova Siskova, Andrej Poljovka, Marcel Zamocky, Eva Vadkerti, Martin Urik, Bence Farkas, Maria Buckova, Lucia Krakova, Domenico Pangallo
Summary: Two different biocleaning techniques were investigated for stamp removal from handmade and machine-made paper samples. The cellulose content in handmade paper and the lignin content in machine-made paper were analyzed. The effectiveness of the biocleaning methods was determined using spectrophotometer measurements and microstructural analysis. The study found that the EEM-based approaches were the most suitable for stamp removal, as they were less time-consuming and easy to produce.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Andrej Poljovka, Milos Musil, David Bednar, Katarina Chovanova, Vladena Bauerova-Hlinkova, Jana Bellova, Lenka Kohutova, Peter Barath, Marcel Zamocky
Summary: The study found that the catalase-peroxidases from thermophilic and mesophilic fungi have similar active center structures and peroxidase activity, while the thermophilic variant CthedisKatG showed enhanced manganese peroxidase activity at higher temperatures. Two constructed mutations completely abolished catalatic activity and altered the peroxidase activity, suggesting the potential biotech applications of bifunctional heme enzymes with variable reactivity.
Article
Biochemistry & Molecular Biology
Marcel Zamocky, Peter Ferianc
Summary: This study demonstrates the deep evolutionary origin of the conserved animal serum amyloid A (SAA) apolipoprotein family, which also leads to the occurrence of highly similar SAA-like sequences in certain bacterial genomes. Horizontal gene transfer of corresponding genes between gut bacteria and non-vertebrate animals was discovered in the phylogenetic tree analysis. The study discusses the conserved sequence motifs and structural fold of the SAA protein family and their potential biological functions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
