Article
Cell Biology
Albin A. Bernardin, Sarah Colombani, Antoine Rousselot, Virginie Andry, Yannick Goumon, Helene Delanoe-Ayari, Come Pasqualin, Bernard Brugg, Etienne D. Jacotot, Jean-Luc Pasquie, Alain Lacampagne, Albano C. Meli
Summary: This study presents an innovative neuro-cardiac organ-on-chip model to study the neuro-cardiac junction (NCJ) and understand the mechanisms involved in its establishment. The model successfully recapitulates the structural and functional properties of the NCJ and allows for the evaluation of the interaction between the heart and brain, as well as the impact of drugs on a reconstructed human neuro-cardiac system.
Article
Biochemistry & Molecular Biology
Maria G. Otero, Shaughn Bell, Alexander H. Laperle, George Lawless, Zachary Myers, Marian A. Castro, Jaquelyn M. Villalba, Clive N. Svendsen
Summary: Conventional tissue culture platforms limit the study of cell biology due to lack of maturity, while organ-chip microfluidic systems provide a more physiological environment for cell growth. In this study, organ-chip cultures showed a higher proportion and homogeneity of mature dopamine neurons compared to multi-well cultures. These organ-chips are ideal for studying the biology of mature dopamine neurons in health and neurological disorders.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
Kornel Kistamas, Anna Muller, Suchitra Muenthaisong, Federica Lamberto, Melinda Zana, Martin Dulac, Filipa Leal, Ali Maziz, Pedro Costa, Eiva Bernotiene, Christian Bergaud, Andras Dinnyes
Summary: Cardiovascular diseases are the leading cause of death worldwide. Various strategies have been developed to treat or prevent cardiac disorders, including small molecules, gene therapy, surgeries, and cardiac rehabilitation. The application of induced pluripotent stem cell-derived human cardiomyocytes shows promise, but their clinical use is hindered by their immature characteristics. Researchers have developed multifactorial approaches that combine biotechnology, life sciences, and material sciences toolsets to induce proper maturation of these cardiomyocytes.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Cell Biology
Mingchong Yang, Ji-Dong Fu, Jizhong Zou, Divya Sridharan, Ming-Tao Zhao, Harpreet Singh, Judith Krigman, Mahmood Khan, Gang Xin, Nuo Sun
Summary: This article discusses how defective mitophagy contributes to normal aging, as well as neurodegenerative and cardiovascular diseases. By studying mitophagy in hiPSCs, valuable insights can be gained to bridge the gap between animal models and human physiology, and hiPSC-CMs can serve as important resources for disease modeling and drug screening.
Article
Biochemical Research Methods
Kyla Bourque, Cara Hawey, Jace Jones-Tabah, Darlaine Petrin, Ryan D. Martin, Yi Ling Sun, Terence E. Hebert
Summary: In left ventricular hypertrophy, the heart thickens its walls to maintain normal cardiac output and function in the presence of coronary artery disease or hypertension. This adaptation initially serves as a protective mechanism but can impair blood distribution as the walls become stiffer. Various model systems, including immortalized cardiomyocyte cell lines and animal models, have been used to study the molecular drivers of hypertrophy. Induced pluripotent stem cell-derived cardiomyocytes provide access to human samples that are directly relevant to hypertrophic cardiomyopathies. This review provides an overview of in vitro and in vivo models used to study hypertrophy and detailed methods for isolating primary neonatal rat cardiomyocytes and generating cardiomyocytes from human iPSCs.
Article
Cell Biology
Antonia T. L. Zech, Maksymilian Prondzynski, Sonia R. Singh, Niels Pietsch, Ellen Orthey, Erda Alizoti, Josefine Busch, Alexandra Madsen, Charlotta S. Behrens, Moritz Meyer-Jens, Giulia Mearini, Marc D. Lemoine, Elisabeth Kraemer, Diogo Mosqueira, Sanamjeet Virdi, Daniela Indenbirken, Maren Depke, Manuela Gesell Salazar, Uwe Voelker, Ingke Braren, William T. Pu, Thomas Eschenhagen, Elke Hammer, Saskia Schlossarek, Lucie Carrier
Summary: This study evaluated the impact of ACTN2 gene variants on cardiomyocyte structure and function. The results showed that the variants led to abnormal cellular phenotypes, including multinucleation, protein aggregation, hypertrophy, and myofibrillar disarray. Additionally, the variants activated proteolytic systems and impaired cardiac force. This study highlights the cellular pathology caused by ACTN2 gene variants and its importance in understanding ACTN2-associated cardiomyopathies.
Article
Cell & Tissue Engineering
Antonio Rampoldi, Parvin Forghani, Dong Li, Hyun Hwang, Lawrence Christian Armand, Jordan Fite, Gene Boland, Joshua Maxwell, Kevin Maher, Chunhui Xu
Summary: In this study, the response of human cardiac progenitors to space microgravity was evaluated. It was found that space microgravity increased the proliferation of hiPSC-cardiomyocytes and improved their structure and function.
Article
Nanoscience & Nanotechnology
Syed Baseeruddin Alvi, Divya Sridharan, Shridhar K. Sanghvi, Muhamad Mergaye, Uzair Ahmed, Sarah K. Mikula, Harpreet Singh, Mahmood Khan
Summary: This study demonstrates the potential of polydopamine nanoparticles (PDA-NPs) as a bioactive agent to enhance contractility and beat propagation of cardiomyocytes. PDA-NPs improve mitochondrial bioenergetics and have cardiotonic effects on the heart. In addition, they can modulate the genetic expression of cardiomyocytes.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Medicinal
Mark Johnson, Jorge Gomez-Galeno, Daniel Ryan, Karl Okolotowicz, Wesley L. McKeithan, Kevin J. Sampson, Robert S. Kass, Mark Mercola, John R. Cashman
Summary: In the United States, approximately one million individuals are hospitalized every year for arrhythmias, making arrhythmias one of the top causes of healthcare expenditures. Mexiletine is currently used as an antiarrhythmic drug but has limitations. The purpose of this work was to identify an analog of mexiletine with superior drug-like properties using normal and Long QT syndrome Type 3 (LQTS3) patient-derived human induced pluripotent stem cell (iPSC)-derived cardiomyocytes. The optimization of substituted racemic pyridyl phenyl mexiletine analogs resulted in a more potent sodium channel inhibitor with greater selectivity for the sodium over the potassium channel and for late over peak sodium current.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Multidisciplinary Sciences
Kenji Miki, Kohei Deguchi, Misato Nakanishi-Koakutsu, Antonio Lucena-Cacace, Shigeru Kondo, Yuya Fujiwara, Takeshi Hatani, Masako Sasaki, Yuki Naka, Chikako Okubo, Megumi Narita, Ikue Takei, Stephanie C. Napier, Tsukasa Sugo, Sachiko Imaichi, Taku Monjo, Tatsuya Ando, Norihisa Tamura, Kenichi Imahashi, Tomoyuki Nishimoto, Yoshinori Yoshida
Summary: The study reveals that an estrogen-related receptor gamma (ERR gamma) agonist and an S-phase kinase-associated protein 2 inhibitor enhance the maturation of cardiomyocytes (CMs) derived from human induced pluripotent stem cells (hiPSCs) by promoting larger cell size, longer sarcomere length, the presence of transverse tubules, and improved metabolic, contractile, and electrical properties.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Ching Ying Huang, Martin W. Nicholson, Jyun Yuan Wang, Chien Yu Ting, Ming Heng Tsai, Yu Che Cheng, Chun Lin Liu, Darien Z. H. Chan, Yi Chan Lee, Ching Chuan Hsu, Yu Hung Hsu, Chiou Fong Yang, Cindy M. C. Chang, Shu Chian Ruan, Po Ju Lin, Jen Hao Lin, Li Lun Chen, Marvin L. Hsieh, Yuan Yuan Cheng, Wan Tseng Hsu, Yi Ling Lin, Chien Hsiun Chen, Yu Hsiang Hsu, Ying Ta Wu, Timothy A. Hacker, Joseph C. Wu, Timothy J. Kamp, Patrick C. H. Hsieh
Summary: In this study, a population-based hiPSC drug screening platform is established to assess toxicity. Thirteen HLA-homozygous super donors are identified after recruiting 1,000 healthy donors and screening for high-frequency HLA haplotypes, representing at least 477,611,135 of the global population. The differentiated hiPSCs into cardiomyocytes and neurons demonstrate their utility in a high-throughput toxicity screen. Dose-dependent toxicity and functional modulation of hit compounds are validated, and reproducible in vivo drug toxicity results using mouse models are shown. This study shows the feasibility of using a population-based hiPSC drug screening platform to study inter population differences in drug toxicity and adverse drug reactions.
Article
Biochemical Research Methods
Sophie Girardin, Blandine Clement, Stephan J. Ihle, Sean Weaver, Jana B. Petr, Jose C. Mateus, Jens Duru, Csaba Forro, Tobias Ruff, Isabelle Fruh, Matthias Mueller, Janos Voeroes, Magdalena
Summary: Building small and controlled circuits of human induced pluripotent stem cell-derived neurons holds great promise in understanding the fundamental principles of information processing and storing in the brain.
Article
Biotechnology & Applied Microbiology
Hannah Agten, Inge Van Hoven, Samuel R. Viseu, Jasper Van Hoorick, Sandra Van Vlierberghe, Frank P. Luyten, Veerle Bloemen
Summary: This study investigates the regenerative potential of human-induced pluripotent stem cell (iPSC)-derived chondrocytes in gelatin methacryloyl (GelMA) hydrogel for hyaline cartilage production. The study demonstrates that GelMA hydrogel supports the chondrocyte phenotype and that encapsulated iPSC-derived chondrocytes can preserve their matrix formation capacity. The study also shows that differentiation medium can promote the formation of hyaline cartilage-like tissue.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Noemi Toth, Xiao-Hua Zhang, Alexandra Zamaro, Martin Morad
Summary: Mutations in the S4938F-RyR2 gene have been found to cause abnormal Ca2+ signaling and arrhythmia in cardiomyocytes, which may lead to sudden cardiac death or heart failure. The mutations result in smaller L-type Ca2+ currents and activated Ca2+ transients, despite larger caffeine-triggered Ca2+ release signals. This study suggests that the mutations compromise the normal function of Ca2+ release channels and increase the risk of arrhythmia.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Rui Wang, Hao Xu, Bin Tan, Qin Yi, Yanting Sun, Han Xiang, Tangtian Chen, Huiwen Liu, Qiumin Xie, Li Wang, Jie Tian, Jing Zhu
Summary: This study investigated the role and regulatory mechanism of SIRT3 in energy metabolism in HiPSC-CMs. SIRT3 expression was found to increase during the differentiation and maturation of HiPSC-CMs. Knocking down SIRT3 impaired mitochondrial structure, respiration capacity, and fatty acid oxidation, while enhancing glycolysis. On the other hand, pharmacological activation of SIRT3 using honokiol improved mitochondrial ultrastructure and energetics, and promoted oxidative phosphorylation in HiPSC-CMs. Furthermore, SIRT3 regulated energy metabolism by targeting the OPA1-controlled mitochondrial dynamics in HiPSC-CMs.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Review
Engineering, Biomedical
Berivan Cecen, Ayca Bal-Ozturk, Gokcen Yasayan, Emine Alarcin, Polen Kocak, Rumeysa Tutar, Leyla Didem Kozaci, Su Ryon Shin, Amir K. Miri
Summary: This article reviews the natural-based biomaterials used in micro-tissue models of organ-on-a-chip devices, and discusses their characteristics, preparation methods, modification methods, and characterization methods. The article also summarizes recent reports on the applications of these biomaterials in the design and fabrication of micro-tissue models. This information will be helpful for bioengineers in selecting the appropriate biomaterials to meet clinical expectations and improve patient outcomes.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Review
Engineering, Biomedical
Mahmoud A. Sakr, Kabilan Sakthivel, Towsif Hossain, Su Ryon Shin, Sumi Siddiqua, Jaehwan Kim, Keekyoung Kim
Summary: Gelatin methacryloyl (GelMA) is a commonly used hydrogel in tissue engineering and drug delivery due to its excellent functionality and versatile mechanical properties. However, its weak mechanical strength can be improved by blending it with other natural and synthetic biomaterials to enhance its physical properties.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Review
Biochemistry & Molecular Biology
Jinghang Li, Xin Yu, Eduardo Enciso Martinez, Jiaqing Zhu, Ting Wang, Shengwei Shi, Su Ryon Shin, Shabir Hassan, Chengchen Guo
Summary: The development of bioadhesives using naturally derived biopolymers has addressed challenges and made significant progress, leading to their wide applications in healthcare and biomedical fields.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Engineering, Biomedical
Shreya Mehrotra, Bruna Alice Gomes de Melo, Mario Miscuglio, Kiavash Kiaee, Su Ryon Shin, Biman B. Mandal
Summary: In vitro maturation of cardiomyocytes can be achieved through silk-based cardiac tissue and a dynamic perfusion-based mechanical stimulation platform. The silk scaffolds provide a native tissue-like structure and excellent mechanical properties, while the application of gradient compression strains influences cardiomyocyte maturation and gene expression.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Immunology
Liwei Jiang, Mine Yilmaz, Mayuko Uehara, Cecilia B. Cavazzoni, Vivek Kasinath, Jing Zhao, Said Movahedi Naini, Xiaofei Li, Naima Banouni, Paolo Fiorina, Su Ryon Shin, Stefan G. Tullius, Jonathan S. Bromberg, Peter T. Sage, Reza Abdi
Summary: Lymph node-resident stromal cells, particularly the leptin receptor-expressing cells, play a crucial role in immune response regulation. Leptin signaling through the JAK/STAT pathway supports the survival and anti-inflammatory properties of these stromal cells. Deficiency in leptin signaling leads to increased inflammatory responses and lymphatic abnormalities. Restoration of leptin signaling improves LN microarchitecture, reduces intra-LN inflammation, and corrects metabolic abnormalities.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Gyan Prakash, Ahmed Shokr, Niels Willemen, Showkeen Muzamil Bashir, Su Ryon Shin, Shabir Hassan
Summary: Gene therapy has emerged as a potential platform for treating dreaded and rare diseases. Viral vectors, due to their efficient nucleic acid delivery ability, have been widely explored in gene therapy. However, their lack of precision in delivery has led to off-target toxicities. As an alternative, non-viral gene delivery vehicles have been developed and are currently used in various therapies, including the SARS-CoV-2 vaccine.
ADVANCED DRUG DELIVERY REVIEWS
(2022)
Article
Materials Science, Biomaterials
Saptarshi Biswas, Bibhas K. Bhunia, G. Janani, Biman B. Mandal
Summary: In this study, silk fibroin fiber-based formulations were developed and evaluated as a cost-effective potential hemostatic agent with shortened clotting time. The alkaline hydrolyzed silk microfibers showed reduced blood clotting time, prothrombin time, and activated partial thromboplastin time in in vitro experiments, indicating their potential as a promising material for hemostasis.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Engineering, Biomedical
Yogendra Pratap Singh, Joseph Christakiran Moses, Ashutosh Bandyopadhyay, Biman B. Mandal
Summary: The study investigates the potential of using 3D bioprinted inflamed human osteochondral units to screen anti-inflammatory drugs for osteoarthritis. The biomimetic model created through bioprinting accurately mimics the inflammation in the early stages of osteoarthritis, providing a potential high-throughput platform for screening novel anti-inflammatory drugs.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Materials Science, Biomaterials
Rashmi Ramakrishnan, Dimple Chouhan, Harikrishnan Vijayakumar Sreelatha, Sabareeswaran Arumugam, Biman B. Mandal, Lissy K. Krishnan
Summary: Millions of people are affected by full-thickness skin injuries, and researchers have developed a bioactive wound healing matrix to promote skin regeneration. The matrix has been validated through in vitro and in vivo experiments, showing its ability to enhance cell adhesion and proliferation, regulate extracellular matrix synthesis, and promote the formation of keratinized layers during the healing process.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Editorial Material
Engineering, Biomedical
Biman B. Mandal, Chitta R. Patra, Subhas C. Kundu
BIOMEDICAL MATERIALS
(2022)
Review
Engineering, Biomedical
Souradeep Dey, Chitra Jaiswal, Sayanti Shome, Bibrita Bhar, Ashutosh Bandyopadhyay, Kodieswaran Manikumar, Rajat Dadheech, Biman B. Mandal
Summary: Photocrosslinkable biopolymers, especially silk, have become highly valuable in formulating biomedical applications due to their ease of processing, applicability, and tunability. This review article provides a comprehensive overview of the synthesis, formulation, and advancements made in the application of silk-based photocrosslinkable blends, emphasizing their potential in tissue engineering and other healthcare applications.
REGENERATIVE ENGINEERING AND TRANSLATIONAL MEDICINE
(2023)
Article
Materials Science, Biomaterials
Swatilekha Hazra, Souradeep Dey, Biman B. Mandal, Charanya Ramachandran
Summary: Silk films have potential as substrates for culturing human corneal endothelial cells. Despite their higher tensile strength compared to native basement membrane, the expression of extracellular matrix proteins and integrins on silk films is comparable to native tissue, confirming their suitability for engineering corneal endothelial cells for transplantation.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Khadimul Islam, Bibhas K. Bhunia, Gargi Mandal, Bedabara Nag, Chitra Jaiswal, Biman B. Mandal, Akshai Kumar
Article
Nanoscience & Nanotechnology
Samiran Morang, Ashutosh Bandyopadhyay, Biman B. Mandal, Niranjan Karak
Summary: A transparent, self-healable, and highly tough waterborne polyurethane elastomer with amazing mechanical properties has been developed by molecular engineering. The elastomer shows excellent shape recovery, high fracture energy, and good hemocompatibility. Additionally, it retains its mechanical strength during melt re-processability and can be biodegraded with the help of microbes. This material holds great potential as a smart biomaterial and coating for biomedical devices.
ACS APPLIED BIO MATERIALS
(2023)