Review
Biotechnology & Applied Microbiology
Erin LaMontagne, Alysson R. Muotri, Adam J. Engler
Summary: Tissue engineering and disease modeling fields have recognized the importance of creating complex and mature structures in vitro to mimic the in vivo niche. However, current efforts in neural applications, particularly human brain cortical organoids (COs), only achieve fetal functionality and lack vascularization. Recent studies have focused on vascularizing cortical and other organoid types to address these limitations.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Cell Biology
Anderson K. Santos, Sergio Scalzo, Raysa T. V. de Souza, Pedro H. G. Santana, Bruno L. Marques, Lucas F. Oliveira, M. Filho Daniel, Alexandre Hiroaki Kihara, Helton da Costa Santiago, Ricardo C. Parreira, Alexander Birbrair, Henning Ulrich, Rodrigo R. Resende
Summary: Organoid development and organ-on-a-chip are technologies that utilize stem cells to create 3D multicellular structures resembling organs and tissues in vivo. They can be strategically employed for disease modeling, drug screening, and host-pathogen studies.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Chrysanthi-Maria Moysidou, Chiara Barberio, Roisin Meabh Owens
Summary: Research in cell biology heavily relies on in vitro assays and models, with the recent advancement in 3D cell biology and engineering providing more physiologically relevant culture platforms. These platforms, such as scaffold-based systems and organs-on-chips, show great potential in bridging the gap between conventional animal studies and human physiology by allowing for more accurate modeling of specific processes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Chemistry, Analytical
Stian Kogler, Kristina Saeterdal Komurcu, Christine Olsen, Jun-ya Shoji, Froydis Sved Skottvoll, Stefan Krauss, Steven Ray Wilson, Hanne Roberg-Larsen
Summary: Organoids and organ-on-a-chip devices are important tools for studying various aspects of organs. This review focuses on the application of mass spectrometry in studying organoids and organ-on-a-chip-derived material. It discusses the use of liquid chromatography-mass spectrometry (LC-MS) for proteomics, metabolomics/lipidomics, and hormones, as well as mass spectrometry imaging (MSI) of organoids. The analysis has provided insights into the development and disease of organs, potentially replacing or complementing animal models.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Alican Ozkan, Danielle L. Stolley, Erik N. K. Cressman, Matthew McMillin, Thomas E. Yankeelov, Marissa Nichole Rylander
Summary: Understanding the impact of inflammation and cirrhosis on drug metabolism regulation is crucial for personalized treatment strategies in hepatocellular carcinoma (HCC). A novel 3D vascularized HCC on chips (HCCoCs) model is developed to mimic the stiffness of normal or cirrhotic liver and study the effects of inflammation and cytochrome P450-3A4 (CYP3A4) on drug efficacy. The model demonstrates that upregulation of metabolic activity, incorporation of cirrhosis and inflammation, leads to increased vascular permeability and significant variability in chemotherapy response.
Article
Pharmacology & Pharmacy
Zhongyu Li, Jiwen Li, Mei Sun, Lei Men, Enhua Wang, Yiran Zhao, Keke Li, Xiaojie Gong
Summary: In vivo, drug metabolism plays a crucial role in altering drug composition and determining the pharmacological properties of oral drugs. However, existing in vitro models lack the ability to replicate the complexity of drug metabolism, resulting in poor predictive power. The development of organs-on-chip-based microfluidics system offers a new in vitro drug screening platform that can mimic the metabolic process and pharmacological activity of natural products. This study utilized an improved microfluidic device to establish an in vitro co-culture model, which successfully examined the metabolism of ginsenosides and their effectiveness against tumors. The results demonstrated the validity and controllability of this model, highlighting the essential role of hepatic metabolism in determining ginsenosides efficacy.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Engineering, Biomedical
Ya Ren, Xue Yang, Zhengjiang Ma, Xin Sun, Yuxin Zhang, Wentao Li, Han Yang, Lei Qiang, Zezheng Yang, Yihao Liu, Changxu Deng, Liang Zhou, Tianchang Wang, Jingsheng Lin, Tao Li, Tao Wu, Jinwu Wang
Summary: Organoids developed from pluripotent stem cells or adult stem cells are three-dimensional cell cultures that mimic certain biological developmental processes of organs, with promising applications in drug screening, disease modeling, and regenerative repair. The challenges facing organoid construction include breakthroughs in scale size, vascularization, reproducibility, and precise architecture. Bioprinting techniques, with the application of bioinks, have accelerated the process of organoid construction, and a multidisciplinary combination of developmental biology, disease pathology, cell biology, and materials science will help overcome obstacles to further develop real organs.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2021)
Review
Biotechnology & Applied Microbiology
Nehar Celikkin, Dario Presutti, Fabio Maiullari, Ersilia Fornetti, Tarun Agarwal, Alessia Paradiso, Marina Volpi, Wojciech Swieszkowski, Claudia Bearzi, Andrea Barbetta, Yu Shrike Zhang, Cesare Gargioli, Roberto Rizzi, Marco Costantini
Summary: Biomedical research has seen significant growth in academia and industry in the past few decades and is expected to continue growing rapidly in the future. The multidisciplinary nature of biomedical research makes in-depth analysis challenging. Technological advances, such as biofabrication and organ-on-a-chip, have played key roles in enabling this progress.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Engineering, Biomedical
Astha Khanna, Beu P. Oropeza, Ngan F. Huang
Summary: Cardiovascular organ-on-a-chip devices consist of engineered or native functional tissues cultured under controlled microenvironments. These systems use microfabrication and tissue engineering techniques to mimic human physiology. This review discusses the applications of human organ-on-a-chip systems in modeling cardiovascular diseases, drug screening, and personalized medicine, as well as the challenges in achieving large-scale utilization for drug development and personalized therapy.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2023)
Review
Pharmacology & Pharmacy
Jingyu Fu, Hailong Qiu, Cherie S. Tan
Summary: Drug discovery is a costly and complex process, and efficient methods are needed to screen potential compounds and remove toxic substances. The liver plays a crucial role in determining drug efficacy and potential side effects. The liver-on-a-chip platform based on microfluidic technology has gained attention for its ability to predict drug metabolism and hepatotoxicity, as well as study pharmacokinetics and pharmacodynamics when combined with other organ-on-chip systems.
Review
Biotechnology & Applied Microbiology
Hyeon Ryoo, Hannah Kimmel, Evi Rondo, Gregory H. Underhill
Summary: Cellular phenotypes and functional responses are regulated by signals present in their microenvironment. High throughput culture platforms can better recapitulate and analyze these complex signals. Various techniques such as microplates, microarrays, and microfluidic cell culture systems have been developed for high throughput cell culture. Current applications include stem cell sourcing, drug discovery, and personalized medicine.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Materials Science, Biomaterials
Wookyeom Kim, Yunji Lee, Dayoon Kang, Taejeong Kwak, Hwa-Rim Lee, Sungjune Jung
Summary: A physiologically relevant human alveolar lung-on-a-chip model was developed using inkjet bioprinting technology, allowing layer-by-layer culture of mammalian lung tissues on a microfluidic chip. The model maintained the critical properties of a lung barrier and can be applied to various organ models.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Review
Materials Science, Biomaterials
Maedeh Rahimnejad, Fariba Rasouli, Sepideh Jahangiri, Sepideh Ahmadi, Navid Rabiee, Marzieh Ramezani Farani, Omid Akhavan, Mohsen Asadnia, Yousef Fatahi, Sanghoon Hong, Jungho Lee, Junmin Lee, Sei Kwang Hahn
Summary: This article reviews the preparation and characterization of biomimetic membranes and discusses their applications in organ-on-a-chip systems. Biomimetic membranes are considered as crucial components for developing controllable biomimetic bioanalysis systems.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Review
Biochemistry & Molecular Biology
Jong Hwan Sung, Jae Jung Kim
Summary: The skin is the largest organ that provides protection and interacts with other organs in the body, making it important for studying various diseases. In vitro skin models, including organ-on-a-chip technology, offer valuable tools for pharmaceutical, cosmetics, and food industries to understand drug metabolism, dermatological diseases, and the interaction of the skin with other organs.
EXPERT OPINION ON DRUG METABOLISM & TOXICOLOGY
(2023)
Article
Engineering, Biomedical
Drew E. Glaser, Matthew B. Curtis, Peter A. Sariano, Zachary A. Rollins, Bhupinder S. Shergill, Aravind Anand, Alyssa M. Deely, Venktesh S. Shirure, Leif Anderson, Jeremy M. Lowen, Natalie R. Ng, Katherine Weilbaecher, Daniel C. Link, Steven C. George
Summary: A 3D in vitro model of human bone marrow containing perivascular and endosteal niches has been developed using microfluidic and stem cell technologies. This model can replicate in vivo bone marrow function and study dynamic events at the niche level, providing opportunities to advance our understanding of human bone marrow function and drug response.
Article
Oncology
Konstantinos I. Votanopoulos, Andrea Mazzocchi, Hemamylammal Sivakumar, Steven Forsythe, Julio Aleman, Edward A. Levine, Aleksander Skardal
ANNALS OF SURGICAL ONCOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Julio Aleman, Aleksander Skardal
BIOTECHNOLOGY AND BIOENGINEERING
(2019)
Article
Chemistry, Multidisciplinary
Julio Aleman, Sunil K. George, Samuel Herberg, Mahesh Devarasetty, Christopher D. Porada, Aleksander Skardal, Graca Almeida-Porada
Article
Engineering, Biomedical
Kevin Enck, Shiny Priya Rajan, Julio Aleman, Simone Castagno, Emily Long, Fatma Khalil, Adam R. Hall, Emmanuel C. Opara
ANNALS OF BIOMEDICAL ENGINEERING
(2020)
Article
Oncology
Konstantinos Votanopoulos, Steven Forsythe, Hemamylammal Sivakumar, Andrea Mazzocchi, Julio Aleman, Lance Miller, Edward Levine, Pierre Triozzi, Aleksander Skardal
ANNALS OF SURGICAL ONCOLOGY
(2020)
Article
Chemistry, Analytical
Erin Maloney, Casey Clark, Hemamylammal Sivakumar, KyungMin Yoo, Julio Aleman, Shiny A. P. Rajan, Steven Forsythe, Andrea Mazzocchi, Adrian W. Laxton, Stephen B. Tatter, Roy E. Strowd, Konstantinos Votanopoulos, Aleksander Skardal
Article
Engineering, Biomedical
Zuyan Lu, Shiny Amala Priya Rajan, Qianqian Song, Yu Zhao, Meimei Wan, Julio Aleman, Aleksander Skardal, Colin Bishop, Anthony Atala, Baisong Lu
Summary: Generating human induced hepatocytes from fibroblasts provides a potential solution for the shortage of primary hepatocytes, enabling personalized drug prediction. Combining 3D scaffold-free microliver spheroids with a microfluidic system allows for drug evaluation and interaction studies between liver cells and tumor cells.
Article
Biotechnology & Applied Microbiology
Brady Trevisan, Alshaimaa Morsi, Julio Aleman, Martin Rodriguez, Jordan Shields, Diane Meares, Andrew M. Farland, Christopher B. Doering, H. Trent Spencer, Anthony Atala, Aleks Skardal, Christopher D. Porada, Graca Almeida-Porada
Summary: The study utilized microfluidic technology to simulate physiological shear stress in liver sinusoids and investigate the effects of shear stress on an innovative biological approach for treating Hemophilia A. The research revealed novel pathways through which endogenous FVIII and vWF are affected by shear stress.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemical Research Methods
Julio Aleman, Tugba Kilic, Luis S. Mille, Su Ryon Shin, Yu Shrike Zhang
Summary: This article details the integration of sensors on organ-on-chips for continuous monitoring of biomarkers, along with the fabrication and utilization of related technologies.
Article
Cell & Tissue Engineering
Ashkan Shafiee, Elham Ghadiri, Jareer Kassis, Nima Pourhabibi Zarandi, Anthony Atala
CURRENT STEM CELL REPORTS
(2018)
Meeting Abstract
Cell & Tissue Engineering
N. Pourhabibi Zarandi, G. Galdon, H. Sadri-Ardekani, A. Atala
TISSUE ENGINEERING PART A
(2017)
Article
Engineering, Biomedical
Aleksander Skardal, Julio Aleman, Steven Forsythe, Shiny Rajan, Sean Murphy, Mahesh Devarasetty, Nima Pourhabibi Zarandi, Goodwell Nzou, Robert Wicks, Hooman Sadri-Ardekani, Colin Bishop, Shay Soker, Adam Hall, Thomas Shupe, Anthony Atala