Article
Chemistry, Multidisciplinary
Yan Zhang, Lei Kang, Ivy H. M. Wong, Weixing Dai, Xiufeng Li, Ronald C. K. Chan, Michael K. Y. Hsin, Terence T. W. Wong
Summary: CHAMP is a promising histological imaging method that enables high-throughput and label-free imaging of tissues with high resolution and processing speed. By unsupervised learning, CHAMP images can be transformed into virtually stained histological images, extracting significant cellular features with high accuracy.
Article
Multidisciplinary Sciences
Chenxi Qian, Kun Miao, Li-En Lin, Xinhong Chen, Jiajun Du, Lu Wei
Summary: VISTA is a label-free superresolution method combining sample expansion and vibrational imaging, enabling high-resolution interrogation of protein-rich biological structures in cells and tissues, with a resolution down to 78 nm.
NATURE COMMUNICATIONS
(2021)
Review
Optics
Natan T. Shaked, Stephen A. Boppart, Lihong V. Wang, Juergen Popp
Summary: This Review provides a comprehensive overview and comparison of different label-free imaging modalities, aiming to facilitate collaboration, technological advancements, and biophysical discoveries, as well as new avenues in clinical detection, diagnosis, and monitoring of diseases.
Article
Chemistry, Multidisciplinary
Yi-Teng Hsiao, Chia-Ni Tsai, Te-Hsin Chen, Chia-Lung Hsieh
Summary: Chromatin is a densely packed DNA-protein complex in the cell nucleus, with nanoscale chromatin compaction playing critical roles in cell nuclear processes. Using DYNAMICS imaging allows for mapping chromatin organization in live cell nuclei, detecting and quantifying chromatin compaction level at nanoscale, and effectively observing chromatin condensation and decondensation processes. This label-free imaging technique offers the opportunity to investigate chromatin conformational dynamics and their significance in gene activities.
Article
Chemistry, Multidisciplinary
Yi-Teng Hsiao, Chia-Ni Tsai, Te-Hsin Chen, Chia-Lung Hsieh
Summary: Chromatin, a DNA-protein complex densely packed in the cell nucleus, plays critical roles in cell nuclear processes. DYNAMICS imaging is a new strategy for mapping chromatin organization in live cell nuclei by analyzing the dynamic scattering signal of molecular fluctuations. This technique allows for accurate measurement of chromatin compaction levels in live cells and effective detection of chromatin condensation and decondensation processes.
Article
Biochemistry & Molecular Biology
Raluca Borlan, Olga Soritau, Dana Maniu, Alexandru-Milentie Hada, Adrian Florea, Simion Astilean, Monica Focsan
Summary: This paper reports easy-to-use and cost-effective nano-sized sensors for the selective and quantitative detection of copper ion based on fluorescence quenching. Glutaraldehyde cross-linked albumin nanoparticles with tunable ultraviolet-to-red autofluorescence emissions are developed as dual-agents for sensing and imaging. These albumin nanoparticles show great selectivity towards copper ion and a low limit of detection, making them suitable for sensing applications in clinical diagnosis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Tetyana Ignatova, Sajedeh Pourianejad, Xinyi Li, Kirby Schmidt, Frederick Aryeetey, Shyam Aravamudhan, Slava Rotkin
Summary: This study developed a multidimensional optical imaging technique to map the distribution of doping and strain in two-dimensional materials. Vertical heterostructures were used for label-free drug detection, and the non-uniform broadening of multimodal signal components was found to be correlated with the local optical properties of the heterostructure.
Article
Cell Biology
Ghazal Naseri Kouzehgarani, Mikhail E. Kandel, Masayoshi Sakakura, Joshua S. Dupaty, Gabriel Popescu, Martha U. Gillette
Summary: The dynamic nature of astrocyte morphology plays an important role in hippocampal learning and memory, particularly in the dentate gyrus. Traditional microscopy techniques lack sufficient contrast to detect changes in astrocyte structure and function, but gradient light interference microscopy allows for quantitative measurements of single-cell dry mass and volume. It has been discovered that the volume of astrocytes decreases by 25% during the nocturnal cycle, primarily through exchange with the aqueous medium in the environment.
Review
Chemistry, Multidisciplinary
Andrei V. Kabashin, Vasyl G. Kravets, Alexander N. Grigorenko
Summary: Label-free optical biosensing has great potential in biomedical diagnostics, environmental and food safety, and security. However, it lacks the sensitivity of traditional labelling methods. This article explores ways to overcome the sensitivity barriers and achieve superior sensitivity in label-free biosensing through novel structural architectures, artificial materials, and the use of optical phase as a sensing parameter.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Multidisciplinary Sciences
Yeon Ui Lee, Shilong Li, G. Bimananda M. Wisna, Junxiang Zhao, Yuan Zeng, Andrea R. Tao, Zhaowei Liu
Summary: The authors demonstrate a label-free superresolution imaging method using a hyperbolic material for tailored light-matter interactions. By enhancing scattering and utilizing dark-field detection, the resolution is improved by 5.5 times beyond the diffraction limit. This method provides capabilities for super-resolution imaging where fluorescence is not available or challenging to apply.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Bjorn Altenburger, Carl Andersson, Sune Levin, Fredrik Westerlund, Joachim Fritzsche, Christoph Langhammer
Summary: This study introduces nanofluidic scattering microscopy to measure the activity of single nanoparticles during catalytic reactions. By detecting minuscule refractive index changes in the liquid, the concentration changes of reactants can be accurately measured. The turnover frequency of the particles is derived from the growth rate of gas bubbles formed during the reaction.
Review
Optics
Vasily N. Astratov, Yair Ben Sahel, Yonina C. Eldar, Luzhe Huang, Aydogan Ozcan, Nikolay Zheludev, Junxiang Zhao, Zachary Burns, Zhaowei Liu, Evgenii Narimanov, Neha Goswami, Gabriel Popescu, Emanuel Pfitzner, Philipp Kukura, Yi-Teng Hsiao, Chia-Lung Hsieh, Brian Abbey, Alberto Diaspro, Aymeric LeGratiet, Paolo Bianchini, Natan T. Shaked, Bertrand Simon, Nicolas Verrier, Matthieu Debailleul, Olivier Haeberle, Sheng Wang, Mengkun Liu, Yeran Bai, Ji-Xin Cheng, Behjat S. Kariman, Katsumasa Fujita, Moshe Sinvani, Zeev Zalevsky, Xiangping Li, Guan-Jie Huang, Shi-Wei Chu, Omer Tzang, Dror Hershkovitz, Ori Cheshnovsky, Mikko J. Huttunen, Stefan G. Stanciu, Vera N. Smolyaninova, Igor I. Smolyaninov, Ulf Leonhardt, Sahar Sahebdivan, Zengbo Wang, Boris Luk'yanchuk, Limin Wu, Alexey V. Maslov, Boya Jin, Constantin R. Simovski, Stephane Perrin, Paul Montgomery, Sylvain Lecler
Summary: This article presents the current state of development in label-free super-resolution imaging, discusses the limitations and challenges in breaking the classical diffraction limit, and proposes new techniques and methods. It brings together researchers from the physics and biomedical optics communities to provide a vision for the future development of label-free super-resolution imaging.
LASER & PHOTONICS REVIEWS
(2023)
Article
Biology
Yulia Beloglazova, Aleksandr Nikitiuk, Anna Voronina, Olga Gagarskikh, Yuriy Bayandin, Oleg Naimark, Victoria Grishko
Summary: This paper introduces a new label-free method for cell viability assessment based on laser interference microscopy, which can monitor the viability of single cells in real-time. By analyzing the viability of attached and suspended cells using various spectral techniques, universal parameters for assessing cell condition have been selected.
Article
Nanoscience & Nanotechnology
Gianluigi Zito, Gennaro Sanita, Bryan Guilcapi Alulema, Sofia N. Lara Yepez, Vittorino Lanzio, Fabrizio Riminucci, Stefano Cabrini, Maria Moccia, Concetta Avitabile, Annalisa Lamberti, Vito Mocella, Ivo Rendina, Silvia Romano
Summary: Research into utilizing all-dielectric metasurfaces sustaining photonic bound states in the continuum for biosensing, combining the potential of reactive sensing principle and topological decoupling for numerical study. Achieved high sensitivity PNA/DNA selective biosensing using quasi-BIC resonator with real-time monitoring of binding events.
Article
Nanoscience & Nanotechnology
Sayan Chandra, Jared Cozart, Aritra Biswas, Sang Lee, Debashis Chanda
Summary: Early detection of IgG is crucial, and the T-MOKE technique shows significantly superior sensitivity and resolving power compared to conventional methods, with a detection limit down to 10 ng/mL. This technique has the potential to be extended to detect biologically/chemically relevant molecules at lower concentrations for early biomedical diagnosis and therapy.
Article
Immunology
Brittany A. Petros, Jacquelyn Turcinovic, Nicole L. Welch, Laura F. White, Eric D. Kolaczyk, Matthew R. Bauer, Michael Cleary, Sabrina T. Dobbins, Lynn Doucette-Stamm, Mitch Gore, Parvathy Nair, Tien G. Nguyen, Scott Rose, Bradford P. Taylor, Daniel Tsang, Erik Wendlandt, Michele Hope, Judy T. Platt, Karen R. Jacobson, Tara Bouton, Seyho Yune, Jared R. Auclair, Lena Landaverde, Catherine M. Klapperich, Davidson H. Hamer, William P. Hanage, Bronwyn L. MacInnis, Pardis C. Sabeti, John H. Connor, Michael Springer
Summary: This study describes the rapid fixation of the Omicron variant in universities and highlights its high transmissibility in small populations. It emphasizes the importance of asymptomatic surveillance programs in early detection of pathogen dynamics.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Immunology
Tara C. Bouton, Joseph Atarere, Jacquelyn Turcinovic, Scott Seitz, Cole Sher-Jan, Madison Gilbert, Laura White, Zhenwei Zhou, Mohammad M. Hossain, Victoria Overbeck, Lynn Doucette-Stamm, Judy Platt, Hannah E. Landsberg, Davidson H. Hamer, Catherine Klapperich, Karen R. Jacobson, John H. Connor
Summary: The majority of Delta- and Omicron-infected cohort culture-converted by day 6, with no further impact of booster vaccination on sterilization or cycle threshold decay. Rapid antigen testing may provide reassurance of lack of infectiousness, though guidance to mask for days 6-10 is supported by our finding that 17% of participants remained culture-positive after isolation.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Multidisciplinary Sciences
Da-Yuan Chen, Chue Vin Chin, Devin Kenney, Alexander H. Tavares, Nazimuddin Khan, Hasahn L. Conway, GuanQun Liu, Manish C. Choudhary, Hans P. Gertje, Aoife K. O'Connell, Scott Adams, Darrell N. Kotton, Alexandra Herrmann, Armin Ensser, John H. Connor, Markus Bosmann, Jonathan Z. Li, Michaela U. Gack, Susan C. Baker, Robert N. Kirchdoerfer, Yachana Kataria, Nicholas A. Crossland, Florian Douam, Mohsan Saeed
Summary: The Omicron variant of SARS-CoV-2 is highly immune evasive but less virulent compared to other major viral variants. It has a large number of mutations in the spike protein, which is the main driver of these traits. The virus bearing Omicron S can efficiently escape vaccine-induced immunity, replicate in cells, and cause less severe disease, but its pathogenicity is determined by mutations both in and outside of the spike protein.
Article
Optics
Alex Matlock, Jiabei Zhu, Lei Tian
Summary: Recovering the 3D phase features of complex biological samples has traditionally involved sacrificing computational efficiency and processing time for physical model accuracy and reconstruction quality. However, this study introduces an approximant-guided deep learning framework that overcomes this challenge in a high-speed intensity diffraction tomography system. By training the network on natural image datasets using a physics model simulator-based learning strategy, complex 3D biological samples can be robustly reconstructed. This framework utilizes a lightweight 2D network structure with a multi-channel input to encode axial information, achieving highly efficient training and prediction.
Article
Virology
RuthMabel Boytz, Scott Seitz, Emily Gaudiano, J. J. Patten, Patrick T. T. Keiser, John H. H. Connor, Arlene H. H. Sharpe, Robert A. A. Davey
Summary: Reliable inactivation methods are essential for virus research and development of diagnostics, vaccines, and therapeutics. This study validates the use of gamma irradiation to inactivate culture supernatants and cell pellets of SARS-CoV-2 and Ebola virus. The required doses for virus inactivation are reported, and the method allows for analysis of protein-based host responses to infection.
Article
Multidisciplinary Sciences
Elif Seymour, M. Selim Unlu, John H. Connor
Summary: Using a single-particle interferometric reflectance imaging sensor (SP-IRIS), we screened 30 monoclonal antibodies (mAbs) against Ebola, Sudan, and Lassa viruses to detect whole viruses. We also developed a model to map the antibody epitopes on the viral surface glycoproteins (GPs). The SP-IRIS results showed that mAbs binding to the mucin-like domain or glycan cap of the GP had the highest signal for Ebola detection. For Lassa virus, the 8.9F antibody performed the best. Additionally, we demonstrated a novel antibody competition assay using SP-IRIS to explain the higher efficacy of the ZMapp cocktail compared to other cocktails against Ebola infection.
SCIENTIFIC REPORTS
(2023)
Article
Biochemical Research Methods
Jelena Platisa, Xin Ye, Allison M. Ahrens, Chang Liu, Ichun Anderson Chen, Ian G. Davison, Lei Tian, Vincent A. Pieribone, Jerry L. Chen
Summary: Monitoring spiking activity in large neuronal populations is crucial for understanding neural circuit function. Voltage imaging provides a new approach for this, but it faces challenges such as reduced fluorescence detection and limited imaging duration. This study developed improved voltage indicators, a high-speed two-photon microscope, and denoising software, enabling simultaneous high-speed deep-tissue imaging of more than 100 labeled neurons over 1 hour. This scalable approach offers a way to image voltage activity across increasing neuronal populations.
Article
Optics
Hao Wang, Jiabei Zhu, Jangwoon Sung, Guorong Hu, Joseph Greene, Yunzhe LI, Seungbeom Park, Wookrae Kim, Myungjun Lee, Yusin Yang, Lei Tian
Summary: Topography measurement is crucial for surface characterization and inspection applications. This study presents a novel topography technique called Fourier ptychographic topography (FPT), which combines a computational microscope and a phase retrieval algorithm to achieve wide-field-of-view and high-resolution topography reconstruction with nanoscale accuracy. FPT has important implications for surface characterization, semiconductor metrology, and inspection applications.
Article
Veterinary Sciences
Nermin Isik-Uslu, Ozlem Derinbay-Ekici, Oguzhan Avci
Summary: This study reports potential causes of diarrhea in neonatal calves in Turkey and identifies the presence of Cryptosporidium spp., rotavirus, coronavirus, and Escherichia coli in fecal samples collected from three provinces.
REVISTA CIENTIFICA-FACULTAD DE CIENCIAS VETERINARIAS
(2023)
Article
Pathology
Anna E. Tseng, Mariano Carossino, Hans P. Gertje, Aoife K. O'Connell, Suryaram Gummuluru, Vijaya B. Kolachalama, Udeni B. R. Balasuriya, John H. Connor, Richard S. Bennett, David X. Liu, Lisa E. Hensley, Nicholas A. Crossland
Summary: The liver in rhesus macaques infected with Ebola virus shows altered myeloid phenotypes, increased neutrophil influx, and activation of interferon responses. These findings provide insights into potential therapeutic strategies for modulating the host's inflammatory response and normalizing hepatic myeloid functionality.
VETERINARY PATHOLOGY
(2023)
Article
Optics
Jian Zhao, Lulu Jiang, Alex Matlock, Yihong Xu, Jiabei Zhu, Hongbo Zhu, Lei Tian, Benjamin Wolozin, Ji-Xin Cheng
Summary: Researchers developed a computational chemical microscope, FBS-IDT, which can extract molecular structure information of amyloid proteins in their native cellular environment. This technology enables label-free volumetric chemical imaging and 3D visualization of amyloid protein aggregates, as well as depth-resolved mid-infrared fingerprint spectroscopy for protein secondary structure analysis. It provides a new approach to study the relationship between neurodegenerative diseases and amyloid proteins.
LIGHT-SCIENCE & APPLICATIONS
(2023)
Article
Immunology
Ludy R. Carmola, Jacquelyn Turcinovic, Garrison Draper, David Webner, Margot Putukian, Holly Silvers-Granelli, Andrei Bombin, Bradley A. Connor, Kristina M. Angelo, Phyllis Kozarsky, Michael Libman, Ralph Huits, Davidson H. Hamer, Jessica K. Fairley, John H. Connor, Anne Piantadosi, Daniel L. Bourque
Summary: MLS used SARS-CoV-2 testing protocol to identify individuals with coronavirus disease 2019. Despite the majority of players being vaccinated, several players still tested positive for SARS-CoV-2, with a family member visiting from England identified as a potential index case. Whole genome sequencing was used to track transmission patterns and identify potential sources of infection.
OPEN FORUM INFECTIOUS DISEASES
(2023)
Article
Multidisciplinary Sciences
Da-Yuan Chen, Jacquelyn Turcinovic, Shuchen Feng, Devin J. Kenney, Chue Vin Chin, Manish C. Choudhary, Hasahn L. Conway, Marc Semaan, Brianna J. Close, Alexander H. Tavares, Scott Seitz, Nazimuddin Khan, Sebastian Kapell, Nicholas A. Crossland, Jonathan Z. Li, Florian Douam, Susan C. Baker, John H. Connor, Mohsan Saeed
Summary: A simple and robust cell culture system is crucial for generating authentic SARSpounds and preparing inactivated vaccines. Vero E6, a commonly used cell line for growing SARS-CoV-2, is not efficient in supporting viral propagation of new variants and leads to rapid cell culture adaptation of the virus. We created a panel of 17 human cell lines overexpressing SARS-CoV-2 entry factors and tested their ability to support viral infection, with two cell lines yielding highly concentrated virus stocks. Interestingly, these cell lines were more sensitive than Vero E6 cells in recovering SARS-CoV-2 from clinical specimens.
Article
Nanoscience & Nanotechnology
Jianing Liu, Hao Wang, Yuyu Li, Lei Tian, Roberto Paiella
Summary: This paper introduces a new type of image sensor that can directly visualize phase objects without additional optical elements. It is particularly significant for applications involving space-constrained and portable setups, and is applicable to surface profiling and biomedical microscopy.